Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)

Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)

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Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)

中文名称:刀豆球蛋白A

英文名:Concanavalin A (Con A)

货号:ML-CONA-1000MG

规格:1 g

High purity Concanavalin A (Con A) lectin has a highly specific carbohydrate binding affinity, for research and in vitro diagnostic analysis.

Affinity purified, lyophilised powder. Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca2+ and Mn2+ for activity.

高纯度刀豆蛋白A(ConA)凝集素具有高度特异性的碳水化合物结合亲和力,用于研究和离体 诊断分析

亲和纯化冻干粉末。Con A不具有血型特异性,对末端α-D-甘露糖和α-D-葡萄糖残基具有亲和力,需要钙的存在。2+2+为了活动。

DESCRIPTION
(1) FORM
Affinity purified, off-white lyophilised powder.
(2) BIOCHEMICAL / PHYSIOLOGICAL PROPERTIES
Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca2+ and Mn2+ for activity. Con A exists in dimeric (pH < 5.6), tetrameric (pH between 5.6 and 7.0) and aggregate (pH > 7.0) forms. An active dimer above pH 5.6 can be generated by succinylation. Con A exhibits mitogenic activity which is dependent upon its degree of aggregation.

(3) PROPERTIES
Activity: 20 μg/mL
Electrophoretic purity: Electrophoresis was preformed using a 14% acrylamide gel.
UV absorbance: In 100 mM NaCl; λmax = 275.8; E1% = 13.7.
Solubility: Slightly hazy, colourless solution at 10 mg/mL in water.
Activity note: Activity is determined using a twofold serial dilution of 1 mg/mL solution of
Con A in PBS* (8.0 g NaCl, 0.3 g KCl, 0.2 g KH2PO4/L; pH 7.2) containing 1
mM Ca2+ and 1 mM Mn2+. The activity is expressed as the lowest concentra-
tion to give agglutination of a 2% suspension of human red blood cells (type O)
in PBS* after 1 hr incubation at room temperature.
(4) STORAGE / STABILITY / SAFETY
Storage temperature: -20ºC
Shelf life: > 3 years at -20ºC
Safety statement: 22-24 / 25

Reference 参考文献:
Entlicher, G.J., Kostir, V. and Kocourek, J. (1971) Studies on phytohemmagglutinins.
8. Isoelectric point and multiplicity of purified concanavalin A. Biochim Biophys Acta 236(3): 795-7