Megazyme K-PULLG6 普鲁兰酶/极限醍醐精检测试剂盒

Megazyme K-PULLG6 普鲁兰酶/极限醍醐精检测试剂盒

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Megazyme是一个全球领先的分析试剂、酶和分析工具。从我们的总部在布雷,Megazyme能够开发、制造、为世界各地的实验室和供应产品。我们的产品发现应用程序在各种不同的行业,包括食品和饮料,生物燃料,动物饲料,葡萄酒和酿酒,奶制品、谷物。

Megazyme K-PULLG6 普鲁兰酶/极限醍醐精检测试剂盒

中文名称:普鲁兰酶/极限醍醐精检测试剂盒

英文名:Pullulanase/Limit-Dextrinase Assay Kit (PULLG6 Method)

货号:K-PULLG6

规格:100 assays (manual)

PULLG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

PullG 6法测定普鲁兰酶采用水溶性底物,即4,6-O-苄基-4-硝基苯-63-α-D-麦芽糖-丙三糖(BPNPG3G3),与α-葡萄糖苷酶和β-葡萄糖苷酶结合.当底物在1,6-α-链上被普鲁兰酶或极限糊精酶水解后,释放出的4-硝基苯基-β-麦芽三糖苷在试剂混合物中α-葡萄糖苷酶和β-葡萄糖苷酶的协同作用下,立即水解成葡萄糖和4-硝基酚。反应结束后,加入稀碱,形成酚酸根离子。在400 nm处读取吸光度,得到的吸光度值与普鲁兰酶活性直接相关。

Colourimetric method for the determination of pullanase
or limit-dextrinase

Principle:
(pullulanase/limit-dextrinase)
(1) Benzylidene-G3-(α-1,6)-G3-β-PNP + H2O → Benzylidene-G3
+ G3-β-PNP

(thermostable α-glucosidase and β-glucosidase)
(2) G3-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)

Note: PNP = 4-nitrophenol

Kit size: 100 assays
Method: Spectrophotometric at 400 nm
Total assay time: 10 min for pullanase preparations
30 min for malt extracts containing
limit-dextrinase
Detection limit: 0.18 U/mL for pullulanase preparations
(50-fold dilution)
0.01 U/g for limit dextrinase in milled malt
Application examples: Assay of microbial pullulanase preparations
Measurement of limit-dextrinase in
malt extracts
Method recognition: Novel method

Advantages

  • High sensitivity
  • Suitable for manual and auto-analyser formats
  • No transglycosylation interference
  • Very cost effective
  • All reagents stable for > 1 year after preparation
  • Very specific
  • Simple format
  • Standard included

Megazyme是分析试剂,酶和检测试剂盒的全球供应商,Megazyme能够为世界各地的实验室开发,制造和供应产品。 我们的产品已应用于各种行业,包括食品和饮料,生物燃料,动物饲料,葡萄酒和酿造,乳制品和谷物。
详细产品列表:

产品 规格 货号
Available Carbohydrates/Dietary Fiber Assay Kit 100 assays of each component K-ACHDF
Integrated Total Dietary Fiber Assay Kit 100 assays per kit K-INTDF
Rapid Integrated Total Dietary Fiber Assay Kit 100 assays per kit K-RINTDF
Total Dietary Fiber Controls Sufficient for 6 Controls K-TDFC
Starch Damage Assay Kit 200 assays per kit K-SDAM
Resistant Starch Assay Kit 100 assays per kit K-RSTAR
Resistant Starch Control Flours 5 Controls per kit K-RSTCL
Total Starch HK Assay Kit 100 assays per kit K-TSHK
Total Starch Control Flours 5 Controls per kit K-TSCK
L-Arabinose/D-Galactose Assay Kit 115 assays (manual) / 1150 assays (microplate) K-ARGA
D-Fructose/D-Glucose Assay Kit 110 assays (manual) / 1100 assays (microplate) K-FRUGL
D-Fructose/D-Glucose Assay Kit (Liquid Ready Reagents) 1100 assays (microplate) K-FRGLQR
The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) 60 assays per kit K-FRGLMQ
L-Fucose Assay Kit 100 assays (manual) / 1000 assays (microplate) K-FUCOSE
D-Gluconic Acid/D-Glucono-δ-lactone Assay Kit 60 assays (manual) / 600 assays (microplate) K-GATE
D-Glucosamine Assay Kit 50 assays (manual) / 500 assays (microplate) K-GAMINE
D-Glucose Assay Kit (GOPOD Format) 660 assays per kit K-GLUC
α-Amylase SD Assay Kit (High Sensitivity Method) 160 / 320 assays (manual) / 640 assays (auto-analyser) K-AMYLSD
β-Amylase Assay (Betamyl-3) 100 / 200 assays per kit K-BETA3
Catalase Assay Kit 100 / 200 assays per kit K-CATAL
Cellulase Assay Kit (CellG3 Method) 180 / 360 assays per kit / 720 (auto-analyser) K-CellG3
Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) 100 assays (manual) / 400 assays (auto-analyser) K-MBG4
β-Glucanase Assay Kit (Malt and Microbial) 100 assays per kit K-MBGL
Glucose Oxidase Assay Kit 200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser) K-GLOX
α-Glucuronidase Assay Kit 50 assays (manual) / 200 assays (microplate) K-AGLUA
Malt Amylase Assay Kit 100 assays (50 of each) per kit K-MALTA
Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method) 100 / 200 assays per kit K-PullG6
Tubes for MegaQuant™ Wave Spectrophotometer (Chemistry Analyzer) 294 tubes (1 pack) D-SFTUBE
MegaQuant Colorimeter Tubes 24 tubes D-MQTUB
Kit Component Organiser 1 Unit D-KITORGANISER