德国 Applichem A7089 DNA-ExitusPlus 核酸污染清除试剂

德国 Applichem A7089 DNA-ExitusPlus 核酸污染清除试剂

AppliChem品牌试剂代理-上海金畔生物科技有限公司
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      德国艾普力(APPLICHEM)代理
      AppliChem一级代理

Product Code:  A7089

Product Name: DNA-ExitusPlus™

Specifications:

  • removes DNA and RNA contaminations from surfaces
    • for cleaning of PCR work stations and equipment
    • for cleaning of electrophoresis equipment, pipetts, reaction tubes etc.
    • delivered as spray bottles or as refill bottles (RF)
    ♦ The special features
    • non-enzymatic degradation of DNA and RNA by catalytic and cooperative effects of the components
    • All components of DNA-ExitusPlus™ are readily biologically degradable and not harmful or toxic for humans.
    • Doesn’t contain aggressive mineralic acids or alkaline substances
    • No toxic fumes. Contains low concentration of alcohol.
    Functionality (Strand breakage test): passes test
    Efficacy (ExitusPlus™ activity test): passes test

Comment:

DNA amplification is one of the most commonly used techniques in the modern research laboratory. The presence of contaminating DNA in and around PCR workstations can lead to unwanted artifacts during amplification. Principally, there are two ways to make DNA not amplifiable:1. by degradation of the DNA (e. g. by the addition of DNases or chemical destruction), or2. by modification of bases – leaving the DNA strand intact, but blocked for reading by polymerases. Using a DNA strand break assay (designed by multiBIND biotec GmbH, Germany), it has been shown that not all DNA decontamination solutions on the market totally degrade DNA! DNA-ExitusPlus™ is an improvement over those products and causes both strand breakages as well as degradation. When used properly at the work area, it totally eliminates the amplification of non-target DNA. DNA-ExitusPlus™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution which is effective on all surfaces.A severe disadvantage of conventional decontamination reagents is revealed in a new test for the corrosive potential of their components. For this purpose, different metal plates were incubated for 20 minutes with identical aliquots of the reagents. The selected metals are representative for equipment and materials found in laboratories. The result of this corrosion test show that all known commercial products contain aggressive chemical substances with corrosive, harmful or even toxic effects. These conventional reagents are known to contain azides, mineralic acids like phosphoric acid or hydrochloric acid, aggressive peroxides or strong alkaline substances like sodium hydroxide. Even after an incubation of only 20 minutes, irreversible damages of the metal surfaces are observed in many cases. The newly developed solution DNA-ExitusPlus™ exhibits its unique characteristics especially in this corrosion test. For all metal surfaces tested, no damage or corrosion is observed after treatment. DNA-ExitusPlus™ was also tested under identical conditions on many different plastic surfaces without any indications of damage.DNA-ExitusPlus™ is a registered trademark of AppliChem GmbH.

Bibliography:

(1)Esser, K.-H. et al. (2006) Nature Methods 3(2), 151DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents.

Storage: RT   protected from light

Nature Methods推荐PCR污染清除方法——德国PanReac AppliChem DNA-ExitusPlus
货号Cat.No.: A7089,0100(100ml)     A7089,0500(500ml)      A7089,1000(1L)

聚合酶链式反应(Polymerase Chain Reaction)简称PCR,是一种选择性体外扩增核酸片段的方法,经过循环复制,扩增产量是指数上升,所以具有极高的灵敏度,被广泛应用于生物和医学的科研,临床各种研究中,是目前应用最为广泛的分子生物学方法之一。
但也正是由于PCR具有极高的灵敏度,微量的核酸污染便会导致假阳性扩增结果而导致实验结果不准确,这也是广大实验者最头痛的问题,如果形成气溶胶污染扩散,则可引起整个PCR实验室的污染,处理起来非常棘手,严重的甚至要关闭实验室。环境中的核酸是主要的污染源,常见的污染源有:
1. 模板提取时真空抽干装置
2. 超净台
3. 电泳装置
4. 紫外分析仪
5. 剪刀、镊子、刀片、移液器等器械
6. 离心机
7. 冰箱门把手,冷冻架,门把手或实验台面等
8. 气溶胶
9. 高压灭菌装置

传统的PCR污染清除方法主要有:稀酸处理法、紫外照射法、尿嘧啶糖苷酶(UNG)法、酶解变性、酒精沉淀等几种方式,但仍然存在无法彻底破坏DNA分子、试剂具有腐蚀性或者毒性、小片段核酸无法清除等不足。

德国AppliChem独家研发的DNA-ExitusPlus是一种无腐蚀、无致癌性的核酸污染清除溶液,该产品可将裸露的核酸完全降解至无法作为扩增模板的小片段,无法被扩增,且无法恢复,从而彻底消除了PCR过程中由于环境污染而导致的假阳性扩增。

DNA-ExitusPlusTM特点:
1. 产品为非酶类试剂;
2. 产品中各组分协同作用,快速、非序列特异性的降解核酸污染;
3. 所有组分可生物降解、无毒无害;
4. 不含有机溶剂或者挥发性物质,不含无机酸或者碱性物质,不会对金属形成腐蚀;
5. 使用简单,喷洒或浸泡处理15分钟即可完成清除作用;
6. 升温至50度可提高反应速度。