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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Vector® Red Substrate Kit, Alkaline Phosphatase (AP)
Description
Vector® Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector® Red substrate can be dehydrated, cleared, and permanently mounted.
Features:
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Ideal for single and multiple labeling
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- One year expiry date
- Sufficient reagents to produce 200 ml of working solution
- Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
- Emission: Above 560 nm
- Color: Red
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Magenta |
| Color of Fluorescence | Red |
| Microscopy | Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 4 ml Vector Red Reagent 1
- 4 ml Vector Red Reagent 2
- 4 ml Vector Red Reagent 3
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I have Vector Red AP substrate kit, SK-5100. The instructions for use suggest making a working solution with 100-200 mM Tris buffer. Can I use a 50 mM Tris buffer instead?
Related Products
BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Hematoxylin Counterstain Catalog #: H-3401-500
Methyl Green Counterstain Catalog #: H-3402-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Levamisole Solution Catalog #: SP-5000-18
Citations
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This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector® Red can be used in IHC/ISH double labeling applications.
The bright red to orange fluorescence of the Vector Red reaction product can be visualized using DyLight® 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.
This kit contains stock solutions in convenient dropper bottles.
