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Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent
Description
Mouse on Mouse (M.O.M.) Biotinylated Anti-Mouse Ig Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector® M.O.M. Immunodetection Kit components. This reagent is supplied as 0.1 ml stock solution sufficient to make 25 ml of working solution.
Features of the M.O.M. Immunodetection system:
- Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
- Clear, crisp specific staining of antigens of interest
- Based on VECTASTAIN® ABC or ImmPRESS® reagent protocols
- Procedures are simple and easy to follow
- No tedious calculations or antibody prebinding steps required
Specifications
Unit Size | 0.1 ml |
---|---|
Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications |
Recommended Storage | 2-8 °C |
Target Species | Mouse |
Conjugate | Biotinylated |
Host Species | Horse |
Format | Concentrate |
Documents
- M.O.M. (Mouse on Mouse) Troubleshooting Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
M.O.M.® (Mouse on Mouse) Blocking Reagent Catalog #: MKB-2213-1
M.O.M.® (Mouse on Mouse) Immunodetection Kit, Basic Catalog #: BMK-2202
VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Avidin/Biotin Blocking Kit Catalog #: SP-2001
Streptavidin, DyLight® 594 Catalog #: SA-5594-1
Streptavidin, DyLight® 488 Catalog #: SA-5488-1
Citations
Technical Information
This is the same reagent contained in the Vector M.O.M. Kits (Cat. Nos. PK-2200, FMK-2201, and BMK-2202).
The Vector M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector M.O.M. Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.
The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.
The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, "Discovery Through Color: A Guide to Multiple Antigen Labeling", available on our website.
Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.