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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
VectaMount® Permanent Mounting Medium
Description
VectaMount Mounting Medium (H-5000) is an optically clear and odorless formula for permanently preserving histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations. VectaMount Mounting Medium contains no toluene or xylene.
Features:
- Permanent, non-aqueous mounting
- Low hazard
- Compatible with horseradish peroxidase, alkaline phosphatase, and glucose oxidase substrates
- Refractive index: 1.49 when dry
Specifications
| Unit Size | 60 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization |
| Recommended Usage | VectaMount Mounting Medium is a non-flammable, non-hazardous, and odorless formula for permanently preserving precipitable enzyme substrates in tissue sections or cell preparations. VectaMount Mounting Medium is compatible with most* clearing agents, including aliphatic hydrocarbon- (e.g. d-limonene) or aromatic hydrocarbon- (e.g. toluene or xylene) based clearing agents.
*For most xylene-free clearing agents such as Clear-Rite 3, incubation times of at least 3x 3-5 minutes is recommended. |
| Recommended Storage | Room Temperature |
| Mounting | Non-Aqueous (Permanent) |
| Antifade | No |
Documents
- User Guide
- In Situ Hybridization Detection Protocol
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
In some IHC detection reagent instructions, the last step states “dehydrate, clear and mount”. What does this mean?
In a manual IHC application, these steps involve submersion of the slides into a graded ethanol series, immediately followed by submersion into a miscible solution (xylene or alternative), followed by placement of a coverslip over the preparation with a suitable mounting medium. These steps preserve the specimen for subsequent visualization, imaging and archiving. From our experience, we suggest moving the slides in a slide rack through a series of dishes, commencing dehydration at 95% ethanol (2 changes for 2 min ea.), then into 100% ethanol (2x 2 min ea.), then into clearing solution (3x 2 min ea.), followed by mounting (coverslipping).
Related Products
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VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VectaMount® AQ Aqueous Mounting Medium Catalog #: H-5501-60
VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
Citations
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