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Texas Red® Avidin DCS, Cell Sorting Grade
Description
Avidin DCS, Texas Red® labeled, is a cell sorter grade of Texas Red® Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Texas Red® Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentration for staining of cells or tissues is 5-20 µg/ml. |
| Solution | 10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin |
| Maximum Excitation | 595-604 nm |
| Maximum Emission | 606-615 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 2.0 mg active conjugate/ml |
| Conjugate | Texas Red |
| Color of Fluorescence | Red |
| Format | Concentrate |
Documents
- Double Immunofluorescent Labeling Protocol
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
Anti-Avidin Antibody, Biotinylated Catalog #: BA-0300-.5
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
Citations
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- Excitation maximum: 595 nm
- Emission maximum: 615 nm
- Color: Red