Lumiprobe Cy系列荧光染料

Lumiprobe Cy系列荧光染料

Lumiprobe  的详细介绍

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,我公司生产并销售生命科学研究和诊断学应用的优质化学药品。我们的产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学和其它试剂。大部分产品我们都有库存,可以随时发到世界各个地方。

自2006年起,Lumiprobe公司就开始生产和销售生命科学研究及诊断用化学试剂产品,包括荧光染料,寡核苷酸分析用亚磷酰胺(phosphoramidites),点击化学(Click chemicals)及其他的试剂。大多数产品在美国备有现货,5个工作日左右时间即可发货到中国。鉴于我们高效的服务,快捷的供货流程,优质的产品,您会得到无比愉悦的购物体验。中国客户可以通过向我们中国代理商发出采购指令,即可查询产品的库存及到货周期,并可享受完善的售前和售后服务。

简单介绍

Lumiprobe公司 Lumiprobe代理 Lumiprobe中国代理

上海金畔生物是Lumiprobe授权签约代理,代理优势,所有产品货期5-7个工作日。

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,上海金畔生物Lumiprobe官网:http://lumiprobe.jinpanbio.com/可提供Lumiprobe的完整产品线,所有规格产品保证货期5-7工作日到货,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格极具竞争力,而且我们的货期仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

主要用途:
NHS ESTER 适合标记氨基酸
azide 适合标记寡核苷酸和DNA
maleimide 适合标记有半胱氨酸的蛋白和多肽以及其他巯基化分子
alkyne 适合标记寡核苷酸和DNA
hydrazide 适合标记醛和酮
carboxylic acid 非活化的

lumiprobe产品按照不同作用分类:

lumiprobe荧光染料介绍

Lumiprobe 水溶性cy染料

Lumiprobe-Cyanine3.5 NHS ester

Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS

Lumiprobe-Cyanine7 NHS ester

Lumiprobe磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide(水溶性)

Lumiprobe动物活体成像用染料|活体成像

Lumiprobe 炔烃染料 Dye alkynes

Lumiprobe 酰肼染料 Dye hydrazides

Lumiprobe羧酸类染料

Lumiprobe 羧酸染料 Carboxylic acid

Lumiprobe 马来酰亚胺染料 Dye maleimides

Lumiprobe 氨基染料 Amino dyes

Lumiprobe N-羟基琥珀酰亚胺酯染料 Dye NHS esters

Lumiprobe Cy5.5马来酰亚胺 Cyanine5.5 maleimide

Lumiprobe-Cyanine5.5 NHS ester

Lumiprobe荧光素的替代染料–菁染料

Lumiprobe SYBR Green I 染料

Lumiprobe“点击化学”用染料

Lumiproe蛋白质、多肽、核酸等生物分子标记染料

Lumiprobe蛋白质巯基标记用活性染料

lumiprobe蛋白质氨基标记用活性染料

Lumiprobe代理授权书

JinPanBio (上海金畔生物科技有限公司)

 

Lumiprobe  推荐产品

  • dsGreen Gel Staining Solution, 10000×

    dsGreen Gel Staining Solution, 10000×

  • Cyanine5 NHS ester

    Cyanine5 NHS ester

  • Cyanine3 NHS ester

    Cyanine3 NHS ester

  • Sulfo-Cyanine7 amine

    Sulfo-Cyanine7 amine

  • Sulfo-Cyanine3 carboxylic acid

    Sulfo-Cyanine3 carboxylic acid

  • Cyanine3 carboxylic acid

    Cyanine3 carboxylic acid

  • BDP TR

    BDP TR

  • BDP TMR

    BDP TMR

Lumiprobe  产品订购

活性染料Dye azidesDye alkynesDye maleimidesDye hydrazidesCarboxylic acidsAmino dyesNon-fluorescent alkynesNon-fluorescent azidesPhosphoramiditesModified triphosphatesProteomic reagentsqPCR/电泳CPG solid supportsAuxiliary reagents

Lumiprobe产品目录

点击化学(Click Chemistry)

叠氮化合物(Azides)

Azidobutyric acid NHS ester

Azido dye derivatives, 10 mM DMSO solutions

Cy3 azide, 10 mM in DMSO

Cy3.5 azide, 10 mM in DMSO

Cy5 azide, 10 mM in DMSO

Cy5.5 azide, 10 mM in DMSO

Cy7 azide, 10mM in DMSO

5-FAM azide, 10 mM in DMSO

6-FAM azide, 10 mM in DMSO

5-JOE azide, 10 mM in DMSO

5-ROX azide, 10 mM in DMSO

6-ROX azide, 10 mM in DMSO

5-TAMRA azide, 10 mM in DMSO

炔烃类染料(Alkynes )

Alkyne amidite, 5′-terminal

Alkyne amidite, hydroxyprolinol

Alkyne solid support for oligo synthesis

Pentynoic acid STP ester

荧光激活染料(Catalysts and activators)

Ascorbic acid

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

SYBR Green I

Gel staining solution, 10000x

For qPCR, 100x solution

改良三磷酸盐(Modified triphosphates)

Amino-11-ddUTP

Amino-11-dUTP

脂类活性染料(Dye activated esters)

Cy3 NHS ester

Cy3.5 NHS ester

Cy5 NHS ester

Cy5.5 NHS ester

2D DIGE荧光染料试剂盒(2D DIGE dye kits)-能与Ettan Dige (GE Healthcare)配合使用,或替代使用。

Phosporamidites

Alkyne amidite, 5′-terminal

Alkyne amidite, hydroxyprolinol

DMS(O)MT aminolink amidite

定制合成荧光双标记引物(Custom dual-labeled probes)

Lumiprobe  Cyanine染料介绍

Cyanine dyes are molecules containing polymethine bridge between two nitrogen atoms with a delocalized charge:

Generic structure of cyanine dyes

Due to their structure, cyanines have outstandingly high extinction coefficients often exceeding 100,000 Lmol-1cm-1. Different substituents allow to control properties of the chromophore, such as absorbance wavelength, photostability, and fluorescence. For example, absorbance and fluorescence wavelength can be controlled by a choice of polymethine bridge length: longer cyanines possess higher absorbance and emission wavelengths up to near infrared region.

A number of cyanine dyes have been used for life science applications. A series of thiazole and oxazole dyes have been used as DNA- and protein-binding dyes (like TOTO, YOYO, Stains All and others). But the most popular cyanine dyes for life science research were introduced by Alan Waggoner and colleagues of Carnegie Mellon University in early 1990s. The dyes were a modification of cyanine dye Indocyanine Green (ICG) which was used for angiography since 1970s, and they all contain two indolenine rings flanking polymethyne chain. The dyes were found to exhibit low non-specific binding to biomolecules, and have bright fluorescence owing to their huge extinction coefficients and good quantum yields. Once patented, these molecules are now in public domain after expiration of patents of CMU, and are available for purchase from Lumiprobe for research and commercial use as various reactive derivatives, such as NHS esters, maleimides, azides for Click chemistry, and other derivatives.

There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water. There are cases when one of the type of cyanines is desired (see Sulfonated vs non-sulfonated cyanines section below).

Non-sulfonated cyanines

Available non-sulfonated dyes incude Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy stands for ‘cyanine’, and first digit is number of carbon atoms between indolenine groups. Cy2 which is oxazole derivative rather than indolenin, is an exception from this rule. Suffix .5 is added for benzo-fused cyanines. Variation of the structures allows to change fluorescence properties of the molecules, and to cover most important part ot visible and NIR spectrum with several fluorophores.

Structures of non-sulfonated cyanine dyes from Lumiprobe

Most derivatives of non-sulfonated cyanines (except for hydrochlorides of hydrazides and amines) have low aqueous solubility. When these molecules are used for biomolecule labeling, use of organic co-solvent (5-20% of DMF or DMSO) is necessary for efficient reaction. Cyanine dye should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer. When conjugation takes place efficiently, the dye reacts before it precipitates.

Fluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding. Absorbance and fluorescence spectra of non-sulfonated cyanine dyes are plotted below.

Absorbance and emission spectra of non-sulfonated cyanine dyes

Sulfonated cyanines

Sulfonated cyanines include additional sulfo-groups which facilitate dissolution of dye molecules in aqueous phase. Charged sulfonate groups decrease aggregation of dye molecules and heavily labeled conjugates.

Currently available sulfonated cyanines include sulfo-Cy3, sulfo-Cy5, and sulfo-Cy7.

Structures of sulfonated cyanine dyes from Lumiprobe

Sulfonated cyanines are highly water soluble. No organic co-solvent is needed to perform labeling with these reagents.

Sulfonated vs non-sulfonated cyanines

Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. However, there are a few differences in labeling protocols that should be noticed. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, Cy5, Cy7, and 15% for .5 counterparts. Sulfo-Cy reagents can be used in purely aqueous conditions. There is also a difference in purification: when dialysis against water or aqueous buffer is used for purification, sulfo-Cy must be used to achieve efficient removal of unreacted dye material. Reactions with both sulfo- and non-sulfo cyanines can be purified by gel filtration, chromatography (HPLC, FPLC, ion exchange), or electrophoresis.

Comparison between Cy and sulfo-Cy dyes

Sulfonated and non-sulfonated cyanines are interchangeable for the labeling of many classes of targets including:

  • soluble proteins, which are tolerant to addition of organic co-solvent
  • antibodies (use 5-10% of DMSO/DMF)
  • DNA and oligonucleotides
  • peptides
  • many small molecules

Conjugates produced with similar sulfo- and non-sulfonated reagents (for example, sulfo-Cy5 and Cy5) are very similar in their fluorescent properties, and can be used with various fluorescence instrumentation.

Sulfonated cyanines must be used for:

  • sensitive proteins which are denatured by DMF or DMSO
  • protein conjugation when purification is done by dialysis
  • nanoparticles in aqueous soluitons
  • insoluble or hydrophobic proteins

Non-sulfonated cyanines must be used for:

  • reactions in organic media (dichloromethane, acetonitrile)

The following cyanine products are available from Lumiprobe.

 

Fluorophore Reactive form
sulfo-Cy3 NHS ester
azide
carboxylic acid
sulfo-Cy5 NHS ester
azide
alkyne
carboxylic acid
sulfo-Cy7 NHS ester
carboxylic acid
Cy3 NHS ester
maleimide
azide
alkyne
carboxylic acid
Cy3.5 NHS ester
azide
carboxylic acid
Cy5 NHS ester
maleimide
azide
alkyne
hydrazide
amine
carboxylic acid
Cy5.5 NHS ester
maleimide
azide
alkyne
carboxylic acid
Cy7 NHS ester
maleimide
azide
amine
carboxylic acid