美国Bio-rad伯乐PROTEAN II xi/XL 垂直电泳槽

美国Bio-rad伯乐PROTEAN II xi/XL 垂直电泳槽

PROTEAN II xi 系统组件

  1. 电泳槽及盖(Tank and lid)
  2. 中央制冷芯(Central cooling core)
  3. 托架(黑色) (Latch (black)
  4. 灌胶架(Casting stand)
  5. 三明治夹(Sandwich clamps)
  6. 胶板对位卡(Alignment card)
  7. 电泳梳(Combs)

PROTEAN II xi 电泳槽和PROTEAN II XL 宽式电泳 槽功能灵活,可用于多种分离,包括SDS-PAGE、第 一和第二向双向电泳、非变性、制备、梯度和高分辨 率核酸琼脂糖电泳。单向电泳( 1-D) 可使用 PROTEAN II xi 电泳槽进行16 x 16 cm 和16 x 20 cm 凝胶电泳。双向电泳(2-D),使用PROTEAN II XL 电 泳槽电泳可容纳17和18 cm IPG 胶条的第二向凝 胶(约18.5 x 20 cm)。

  • 灌胶架上专利的单螺纹夹*可在全长玻板上均 匀施加压力,防漏密封设计,无需封脂类或 琼脂糖
  • 中央制冷芯可外接水循环或一个冷却再循环 水浴,或灌装冷却剂作为散热器使用。只需 要1.5 L 缓冲液就可以保证凝胶结果不会出现 “微笑”现象。
  • 可选择两种长度的凝胶:16 或20 cm,根据 所选的凝胶长度选择相应的玻板、封边垫条和三 明治夹(所有其他组件均要与相应的凝胶长度匹配)
  • 使用495 型梯度生成仪、PROTEAN II 多 板凝胶灌胶器和合适的对数活塞可灌制多种梯度胶;容量为7-50 ml 的475 型梯度生成系统与PROTEAN II 板式凝胶灌 胶架是灌制单一梯度凝胶的理想选择
  • 使用带有凹口的玻板(notched plates),可同时进行4 块16 x 16 cm 或16 x 20 cm 手灌胶电泳,可增加一次电泳的样品数(不推荐用于双向电泳)
  • 可选择磨砂玻板(frosted glass)进行高分辨率的垂直琼脂糖核酸电泳;电泳 梳转换螺纹夹(comb conversion screws) 可把任何PROTEAN II xi 电泳梳从标 准的25 mm 孔深转变为10 mm 孔深,并方便的将电泳梳从易碎琼脂糖凝胶中 取出

应用于双向电泳的PROTEAN II IPG 转换组合套件
PROTEAN II IPG 转换组合套件可把经典的PROTEAN II xi 电泳槽和多板电泳槽规格扩大为XL 格式,以适用于 IPG 胶条的第二向电泳

  • 凝胶宽度增加到18.5 cm,适合17 cm 及18 cm ReadyStrip IPG 胶条
  • 转换组合套件包括凝胶夹(gel clamps)、封边垫条(Spacers)、2-D电泳梳(2-D combs)、密封衬垫(gaskets)和玻板(glass plates),适合1.0 mm、1.5 mm或2.0 mm厚度的凝胶
  • PROTEAN II XL 电泳槽包括PROTEAN II IPG 转换组合套件

Specifications for PROTEAN II xi XL 垂直电泳槽

PROTEAN II xi
(16 cm)
PROTEAN II xi
(20 cm)
PROTEAN II XL
(20 cm)
凝胶大小(W x L) 16 x 16 cm 16 x 20 cm 18.5 x 20 cm
玻板规格(W x L)
  内板 16 x 20 cm 20 x 20 cm 20 x 20 cm
  外板 18.3 x 20 cm 22.3 x 20 cm 22.3 x 20 cm
封边垫条长度 18.3 cm 22.3 cm 22.3 cm
典型上层缓冲液容积 350 ml 350 ml 350 ml
典型下层缓冲液容积 1.8 L 1.2 L 1.2 L
典型SDS-PAGE电泳时间*
  无冷却 4 小时 5 小时 5 小时
  带冷却 2.5 小时 3.5 小时 3.5 小时

定购信息

目录 描述
PROTEAN II xi Cells
165-1801 PROTEAN II xi Cell, 16 cm, without spacers and combs
165-1802 PROTEAN II xi Cell, 16 cm, 1.5 mm spacers (4), 15-well combs (2)
165-1803 PROTEAN II xi Cell, 16 cm, 1.0 mm spacers (4), 15-well combs (2)
165-1804 PROTEAN II xi Cell, 16 cm, 0.75 mm spacers (4), 15-well combs (2)
165-1811 PROTEAN II xi Cell, 20 cm, without spacers and combs
165-1812 PROTEAN II xi Cell, 20 cm, 1.5 mm spacers (4), 15-well combs (2)
165-1813 PROTEAN II xi Cell, 20 cm, 1.0 mm spacers (4), 15-well combs (2)
165-1814 PROTEAN II xi Cell, 20 cm, 0.75 mm spacers (4), 15-well combs (2)
165-1815 PROTEAN II xi Cell 2-D Conversion Kit, includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets, grommets, stoppers
165-1834 PROTEAN II xi Basic Unit With Casting Stand, includes central cooling core, lower buffer chamber, lid with cables, leveling bubble; combine with an IPG conversion kit for a complete 18.5 cm wide-format system
PROTEAN II XL Cells Compatible With IPG Strips
165-3188 PROTEAN II XL Cell, wide format, 1.0 mm, includes PROTEAN II xi basic unit (catalog #165-1834) and 1.0 mm IPG conversion kit (catalog #165-3183)
165-3189 PROTEAN II XL Cell, wide format, 1.5 mm, includes PROTEAN II xi basic unit (catalog #165-1834) and 1.5 mm IPG conversion kit (catalog #165-3186)
165-3190 PROTEAN II XL Cell, wide format, 2.0 mm, includes PROTEAN II xi basic unit (catalog #165-1834) and 2.0 mm IPG conversion kit (catalog #165-3184)
IPG Conversion Kits
165-3183 PROTEAN II xi Cell IPG Conversion Kit, 1.0 mm, 18.5 x 20 cm, includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs, 2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card
165-3184 PROTEAN II xi Cell IPG Conversion Kit, 2.0 mm, 18.5 x 20 cm, includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs, 2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card
165-3186 PROTEAN II xi Cell IPG Conversion Kit, 1.5 mm, 18.5 x 20 cm, includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG 2-D combs, 2 IPG central cooling core gaskets, 2 casting stand gaskets, alignment card