For research use only!

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₅H₂₃N₅O₁₆P₂

Molecular Weight: 591.32 g/mol

Exact Mass: 591.06 g/mol

Purity: ≥ 98 % (HPLC)

Form: solid

Color: white to off-white

Solubility: water, aqueous buffer such as PBS

Applications:
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 ß-GT)^[1-3]
The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice

• to introduce a (Desthio)Biotin group for subsequent purification tasks (via DBCO-containing (Desthio)Biotin)
• to introduce fluorescent group for subsequent microscopic imaging (via DBCO-containing fluorescent dyes)

0.5 mg UDP-6-azide-glucose are sufficient to prepare 283 μl of a 3 mM solution.应用：
5-羟甲基胞嘧啶 (5-hmC) 与 T4 ß-糖基转移酶 (T4 ß-GT) 的糖基化 [1-3]
生成的叠氮化物功能化、糖基化的 5-hmC 部分随后可以通过提供选择的无 Cu(I) 点击化学进行检测

为后续纯化任务引入 (Desthio)Biotin 基团（通过含 DBCO 的 (Desthio)Biotin）
为随后的显微成像引入荧光基团（通过含 DBCO 的荧光染料）
0.5 mg UDP-6-azide-glucose 足以制备 283 μl 的 3 mM 溶液。

Selected References: 参考文献
[1] Song et al. (2011) Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nature Biotech 29(1):68.
[2] Li et al. (2012) Selective Capture of 5-hydroxymethylcytosine from Genomic DNA. J. Vis. Exp. 68:e4441.
[3] Song et al. (2016) Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. PNAS 113(16):4339.