Lumiprobe FAM azide, 5-isomer
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
| Cat. # | Quantity |
| 14130 | 100 uL, 10 mM/DMSO |
| 34130 | 500 uL, 10 mM/DMSO |
| 44130 | 1 mL, 10 mM/DMSO |
| A4130 | 1 mg |
| B4130 | 5 mg |
| C4130 | 10 mg |
| D4130 | 25 mg |
| E4130 | 50 mg |
| F4130 | 100 mg |
General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Product citations
- Su, H.; Liu, Z.; Liu, Y.; Ma, V.P.-Y.; Blanchard, A.; Zhao, J.; Galior, K.; Dyer, R.B.; Salaita, K. Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules. Nano Letters, 2018, 18(4), 2630–2636. doi:10.1021/acs.nanolett.8b00459
- Long, M.J.C.; Urul, D.A.; Chawla, S.; Lin, H.-Y.; Zhao, Y.; Haegele, J.A.; Wang, Y.; Aye, Y. Precision Electrophile Tagging inCaenorhabditis elegans. Biochemistry, 2018, 57(2), 216–220. doi: 10.1021/acs.biochem.7b00642
- Fatona, A.; Berry, R.M.; Brook, M.A.; Moran-Mirabal, J.M. Versatile Surface Modification of Cellulose Fibres and Cellulose Nanocrystals through Modular Triazinyl Chemistry. Chemistry of Materials, 2018, 30(7), 2424–2435. doi: 10.1021/acs.chemmater.8b00511
- Pink, M.; Verma, N.; Zerries, A.; Schmitz-Spanke, S. Dose-dependent response to 3-nitrobenzanthrone exposure in human urothelial cancer cells. Chemical Research in Toxicology, 2017, 30(10), 1855–1864. doi: 10.1021/acs.chemrestox.7b00174