DEAE-纤维素 DE-52 使用说明

DEAE-纤维素 DE-52 使用说明
DEAE-纤维素(52)
使用说明书
简介
DEAE-纤维素,它采用平均粒径为50μm的颗粒型亲水高分子聚合物,表面又用大
分子糖链接枝,使它有更高的比表面积和更好的生物兼容性,它在高流水下保持
更高载量,同时又具有更好的分辨率。由于比表面积大,平衡和洗脱的时间也更.
短。它经过接枝即使是纯化病毒,质粒等超大分子的物质,载量基本保持不变。

关于DEAE52纤维素的处理只有简单的几步,如下:

1.先将干粉的纤维素浸泡在蒸馏水中,一段时间大约3小时左右,我偏爱这个时间,去除杂质,最好抽干一下;
2.再用0.5mol/l的HCl溶液浸泡2小时,用去离子水洗净至PH中性,并抽干;
3.将抽干的纤维素再浸泡在0.5mol/l的NaOH溶液中2小时,用去离子水洗至中性,抽干。即可用了
对于用过的纤维素,可以重复利用多次,但需要经过再生处理后才可以使用。再生处理可先用高浓度NaCl (1-2 mol/L)过柱冲洗柱床,以除去DEAE-52阴离子交换纤维所吸附的成份。然后,再用0.5 mol/L的HCl和NaOH处理,处理方法与预处理完全相同。

DEAE-纤维素的处理及装柱
(1)处理
本实验采用的是DEAE-纤维素DE 52 是弱酸型阴离子交换剂,具体处理方法为:先将DE52阴离子交换剂干粉浸泡于蒸馏水中,去除杂质;再在0.5N的HCL溶液中浸泡1-2h,再用无离子水或蒸馏水洗至pH值中性或PH4以上,并将其在抽滤漏斗中抽干;将抽干的离子交换剂浸泡在0.5N的NaOH溶液中1-2h,再用无离子水或蒸馏水将其洗至中性。
(2)装柱
将层析柱清洗干净垂直固定到层析架上,加1/3体积的无离子水,打开下出液口,水流畅通,即刻将小烧杯中装有适宜浓度的柱材,轻轻倒入层析柱中,凝胶自然慢慢沉降再层析柱底部,凝胶沉积直到离层析柱上端1.5-2cm处,停止装柱。层析柱上端进液口连接恒流泵,下出口连接蛋白质监测仪,待层析柱的平衡。
(3)平衡

在柱层析上样前必须对层析柱进行平衡,所谓平衡就是将层析柱中的溶液用层析过程的缓冲液(洗脱液)置换出来,使层析柱中的缓冲系统与柱层析过程中的系统一致。其方法是:利用层析柱上端的恒流泵将平衡缓冲液泵入到层析柱内,打开层析柱下端的出口,平衡液流速在0.5-1ml/min,当下出口流出液的PH值与平衡缓冲液的PH值一致时,层析柱达到了平衡。在本实验中,用0.002M Tris-HCL缓冲液PH7.4(内含0.0001M EDTA),预先将DE-52柱进行平衡。

DEAE—纤维素的活化

称取IgDEAE32或52,放入5ml量筒中,加蒸馏水浸泡过夜,观察溶胀后DEAE的体积。根据所需层析柱的柱床体积计算所需DEAE的用量,称取所需DEAE用蒸馏水浸泡过夜,其间换几次水,每次除去细小颗粒。抽干,改用0.5ml/LNaOH溶液浸泡1h以上,抽干(可用布氏漏斗),用无离子水漂洗,使pH至8左右(用pH试纸检查)。再改用0.5ml/LHCl溶液浸泡1h以上,去酸溶液,用无离子水洗至pH6左右。本实验中在用前应以0.0175mol/L,pH6.7磷酸盐缓冲液,浸泡平衡后使用。

DEAE—纤维素的再生
用过的离子交换剂可以反复使用,使其恢复原状的方法俗称“再生”。再生并非每次用酸、碱反复处理,通常只要“转型”处理即可。所谓转型就是使交换剂带上所希望的某种离子,如希望阳离子交换剂带上NH+4则可用NH4OH浸泡,如希望阴离子交换剂带上Cl—则用NaCl溶液处理。本实验中DEAE—纤维素在酸碱处理后,用0.0175mol/L,pH6.7磷酸盐缓冲溶液浸泡即可转型,以HPO4取代DEAE中的OH—。一般由于DEAE—纤维素使用后因带有大量杂蛋白,所以再生时,先用0.5ml/L NaOH浸洗,用去离子水洗至pH8左右,再转型,即可再使用。

三、应用的注意事项:
1)色谱柱装填
1、所需要用到的材料的温度要与色谱操作的温度一样,液体最好做脱气处理。
填料可直接称量需要的量用缓冲液溶涨一小时装柱即可.
2、在柱子下端加入20%乙醇,以除去柱子中的空气,关闭柱子出口,在柱
内保留少量的20%乙醇。20%乙醇容易产生气泡,可以在里面加1%吐温避免气泡产生。也可以换成纯水装柱子,但是需要把填料中的20%乙醇也换成纯水,具体
的方法取需要体积的填料在抽滤漏斗上进行,也可以小心倾去填料上的20%乙
醇,再换成5倍体积的纯水,反复沉淀去上清,5次左右就可以用于装柱子。
3、此填料颗粒比较细,所以一定 要注意柱子要选择合适的筛网,不能漏,
也可以取点填料加到筛网.上试试,如果没有问题再将填料连续倒入柱子时,要用
玻璃棒的紧靠柱子内壁引流,以减少气泡的产生,让填料先自然沉降到填料体积
不再变化,而填料和上面的液体很好分层,上层溶液完全澄清,就可以开泵用适
当的流速压柱子,填料体积不再变化后,再把转换头紧顶在填料上就可以平衡柱
子使用。使用的流速要小于装柱子的流速。
4、在装柱子前,填料从冰箱中取出至少要室温放置2-3个小时,这样避免
装柱子时由于温度变化而使柱子中产生气泡。
2)蛋白的结合
样品的盐浓度和pH要尽量和平衡柱子的缓冲液-致,盐浓度过高或者pH带
低也许挂不上,所以要根据自己的样品做适当调整。
3)蛋白的洗脱
这个填料如果采用线性梯度洗脱,柱子的直径和高度比最好是大于10,数值
越大越有利于分离,而且样品最好别上太多,可以按约10mg/ml.上样,如果采用
阶段洗脱的方法,装短粗柱子就可以,上样量也没有限制。阶段洗脱容易放大,
重复性好,如果洗脱条件好完全可以得到和线性梯度一样或者更好。采用什么方
法完全根据自己需要。
四、再生清洗
1、每次用完最好用含2M NaCl洗5个床体积,再用水洗5个柱床体积,然后用
20%乙醇保存,使用3-5次后在水洗之后再用70%的乙醇或30%异丙醇都含1%吐
温洗5个柱床体积,最后20%的乙醇流洗5个柱床体积。
2、有机溶剂和水混合很容易产生气泡,为了避免这样情况,可以把配好的有
机溶剂在室温放置过夜,再使用,这样可以避免气泡进柱子而导致柱子不能正常
使用。
五、保存
在20%乙醇中,4’C下长期保存。

63410-01-多聚赖氨酸包被载玻片

63410-01-多聚赖氨酸包被载玻片

1.  多聚赖氨酸包被载玻片poly-L-lysine coated slides

包被载玻片所用的多聚赖氨酸的分子量在150000-300000之间。首先配制0.1%的多聚赖氨酸水溶液,贮存于-20℃。包被时,取出溶液由载玻片的一段滴上,使其慢慢印迹,直至完全覆盖玻片。多聚L赖氨酸包被的膜一定要确保很薄,经过风干即可使用。在一般免疫组化实验中,多聚L赖氨酸不影响实验的结果。但是在免疫胶体金-银染的实验中,一定要谨慎,可能会引起沉淀反应。

货号 产品描述 规格
63410-01 Poly-L-Lysine Coated Slides 72/bx
63410-02 Poly-L-Lysine Coated Slides 144/bx

圆形盖玻片,德国玻璃(Round Cover Slips, German Glass)

1.用于神经细胞培养 2. 用于难贴壁细胞培养(会显出很好的贴壁效果)

3. #1的厚度大约0.13-0.16mm

4. 每包装重28克

5. 6孔细胞培养板,以及12孔、24孔细胞培养板,均有适宜规格的圆形盖玻片。

6. 适用于细胞培养板的圆形玻璃片,由美国公司精心打造,满足您实验中的一切需求!

品牌 货号 直径 厚度 片数
Electron Microscopy Sciences 72196-12 12 mm #1 (0.13 to 0.16mm) 650片/箱
Electron Microscopy Sciences 72196-15 15 mm #1 (0.13 to 0.16mm) 378片/箱
Electron Microscopy Sciences 72196-25 25 mm #1 (0.13 to 0.16mm) 160片/箱

Plastic and Glass Microscope Slides

Including Plastic, Glass, Quartz, Permanox™, Polystyrene, PTFE Coated, Poly-L-Lysine, and Silane Treated

Millennia 2.0 Adhesion Slidesarrow13Millennia™ 2.0 Adhesion Slides, 75 x 25 mm

Millennia™ 2.0 Slides have a higher density of binding site, which significantly increases the adhesive forces between the slide and the anchored tissue section; Millennia™ 2.0 Slides are ideal for immunohistochemistry, can tolerate harsh chemicals with no undesirable background staining, tissue artifact, or specimen loss.

71863-01 Millennia 2.0 Adhesion Slide, White 144/pk 59.00 Add to Cart
71863-02 Millennia 2.0 Adhesion Slide, Aqua 144/pk 59.00 Add to Cart
71863-03 Millennia 2.0 Adhesion Slide, Blue 144/pk 59.00 Add to Cart
71863-04 Millennia 2.0 Adhesion Slide, Burnt Orange 144/pk 59.00 Add to Cart
71863-05 Millennia 2.0 Adhesion Slide, Green 144/pk 59.00 Add to Cart
71863-06 Millennia 2.0 Adhesion Slide, Lilac 144/pk 59.00 Add to Cart
71863-07 Millennia 2.0 Adhesion Slide, Pink 144/pk 59.00 Add to Cart
71863-08 Millennia 2.0 Adhesion Slide, Tan 144/pk 59.00 Add to Cart
71863-09 Millennia 2.0 Adhesion Slide, Yellow 144/pk 59.00 Add to Cart

Millennia 2.0 Adhesion Slidesarrow13Diamond Etch™ Adhesion Slides, 90°

Slides which offer optimized etched labeling resolution. White glass, designed with top/bottom contrasting colors, specifically for slide etching instruments. Exhibits higher visibility with contrasting background.

71865-01 Diamond Etch™ Adhesion Slide, White 144/pk 56.00 Add to Cart
71865-02 Diamond Etch™ Adhesion Slide, Blue 144/pk 56.00 Add to Cart
71865-03 Diamond Etch™ Adhesion Slide, Green 144/pk 56.00 Add to Cart
71865-04 Diamond Etch™ Adhesion Slide, Lilac 144/pk 56.00 Add to Cart
71865-05 Diamond Etch™ Adhesion Slide, Pink 144/pk 56.00 Add to Cart
71865-06 Diamond Etch™ Adhesion Slide, Yellow 144/pk 56.00 Add to Cart
71865-07 Diamond Etch™ Non-Adhesion Slide, White 144/pk 38.00 Add to Cart
71865-08 Diamond Etch™ Non-Adhesion Slide, Blue 144/pk 38.00 Add to Cart
71865-11 Diamond Etch™ Non-Adhesion Slide, Pink 144/pk 38.00 Add to Cart
71865-12 Diamond Etch™ Non-Adhesion Slide, Yellow 144/pk 38.00 Add to Cart

arrow13InkPro™ Slides

White glass, specifically designed and optimized for use with ink jet slide printing instruments. Print quality exhibits high resolution bar codes and alphanumerical characters for efficient and error-free scanning.

71866-01 InkPro™ Slide, White 144/pk 23.00 Add to Cart
71866-02 InkPro™ Slide, Aqua 144/pk 23.00 Add to Cart
71866-03 InkPro™ Slide, Blue 144/pk 23.00 Add to Cart
71866-04 InkPro™ Slide, Green 144/pk 23.00 Add to Cart
71866-05 InkPro™ Slide, Lilac 144/pk 23.00 Add to Cart
71866-06 InkPro™ Slide, Pink 144/pk 23.00 Add to Cart
71866-07 InkPro™ Slide, Yellow 144/pk 23.00 Add to Cart

Positive Charge Microsope Slidesarrow13Positive Charge Microscope Slides

These new slides have a positive charge that makes the surface ideal for cell, tissue and general sample adhesion. Typical samples have a negative charge and are therefore attracted to the positively charged slide surface, significantly reducing sample “float”.

Slides are made of high clarity glass, 25 x 75 mm and are 1.1 mm thick (compatible with most printers). Frosted marking surface on one end. Packed 72 pieces per plastic box/1440 per case (20 packs).

71873-01 45° corners positive charged, frosted end 72/pk 20.00 Add to Cart
71873-02 90° corners positive charged, frosted end 72/pk 20.00 Add to Cart

Control Slides Helicobacter Humanarrow13Control Slides Helicobacter Human

Tissue Control Slides; one slide in every pack has a serial section stained using the helicobacter human staining procedure.

71871 Control Slides Helicobacter Human 25/pk 125.00 Add to Cart

HiQa™ Microscope Slidesarrow10HiQa™ Microscope Slides

HiQa™ Microscope Slides are high quality assured. They have 90 degree ground edges for safety and are available as Plain or Twinfrosted, and in Mega and SupaMega sizes.

These high quality slides are cellophane wrapped, pre-cleaned, ready to use, and suitable for use with automatic coverslipping machines.

71881-30 HiQa™ Supa Mega Twinfrost Slide, 1.0-1.2 mm. Sq ground edge 100/pk 43.00 Add to Cart
71881-40 HiQa™ Supa Mega Plain Slide, 1.0-1.2 mm. Sq ground edge 100/pk 39.00 Add to Cart
71881-50 HiQa™ Mega Twinfrost Slide, 1.0-1.2 mm. Sq ground edge 100/pk 37.00 Add to Cart

Histobond® + Supa Mega Slidesarrow10Histobond® + Supa Mega Slides

Histobond® + Supa Mega Slides are designed for mounting large sections of neuro and breast tissue. Manufactured from the highest quality white glass, with an innovative positive charge coating, Histobond® + Supa Mega slides offer exceptional adhesive properties and provide a superior coverage of reagents across the slide.

These slides have a ‘Right Side’ indicator (RS) to help ensure users correctly orientate the slide, as well as a 7 mm marking area across the width of the slide in order to maximize the working area.

71881-60 Histobond® + Supa Mega Slides 75.2 x 50.4 1 mm, White 50/pk 79.00 Add to Cart

HiQa™ Mega and Supa Mega Cover Slipsarrow10HiQa™ Supa Mega Cover Slips

HiQa™ Supa Mega Cover Slips are made from transparent hydrolytic class 1, flaw-free glass.

They are guaranteed to be of the highest quality.

71881-90 HiQa™ Supa Mega Cover Slips No. 1.0, 50 x 64 mm 100/pk 33.00 Add to Cart

Marienfeld Superior Uncoated Soda Lime Glass Microscope Slidesarrow10Marienfeld Superior™ Uncoated Soda Lime Glass Microscope Slides

EMS introduces the Marienfeld Superior Uncoated Soda Lime Glass Microscope Slides. They are used for the preparation and archiving of specimens, as well as for microscopic investigation.

These slides measure 76 mm x 26 mm x 1 mm thick. They are wettable, made from soda lime glass, pre-cleaned, and ready for use.

71872 Soda Lime Glass Slides 50/pk 12.00 Add to Cart

Microscope Glass Slides - Escoarrow13Microscope Glass Slides

Our microscope glass slides are manufactured with reliability and quality to be the best.

  • Made from the highest quality glass sheets
  • The dimensions of the slides have been standardized to millimeters according to the U.S. government Specifications
  • They are packed with care to assure cleanliness
  • The glass is fully annealed to minimize chipping

Our microscope glass slides are precleaned and are made from clear-white glass. They are individually inspected and free from moisture, dirt, and film. All of our slides are flat, uniform, durable, and cellophane wrapped.

Chemical Makeup

Green (%) White (%)
Silicon Dioxide 72.2 72.15
Sodium Oxide 14.5 14.25
Calcium Oxide 6.5 6.25
Magnesium Oxide 4.4 4.1
Aluminum Oxide 1.5 1.12
Potassium Oxide 0.3 1.15
Sulfur Trioxide 0.3 0.3
Iron Oxide 0.1 0.3
Titanium Oxide 0.05 0.05

Physical Properties

Glass Density 2.4024 g/cm3
Softening Point 724°C
Annealing Point 545°C
Strain Point 511°C
Dispersion Value (Nu Value) 64
Dimensional Variance ±1.5000
Thermal Expansion (0-300°C) 8.36 x 106/ °C
Dielectric Constant @ 20°C (68°F) (1 MHz):ET = 6.7
Young’s Modulus E = 10,000,000 lbs/sq in.
Torsion Modulus G = 4,000,000 lbs/sq in.
Poisson’s Ratio µ = 0.2
Luminous Transmittance @ 0.040: 91.8%

arrow13Microscope Slide, Plain End

Catalog # Thickness Size, mm Pack Price
71880-01 1.2 mm 25 x 75 Gross 38.50 Add to Cart
71880-10 1.2 mm 25 x 75 10 Gross 370.00 Add to Cart
71880-20 1.2 mm 25 x 75 20 Gross 699.00 Add to Cart

arrow13Microscope Slide, Frosted End

These slides have 3/4″ frosting on one end which allows for easy marking.

Catalog # Thickness Size,mm Pack Price
71870-01 1.2 mm 25 x 75 Gross 44.00 Add to Cart
71870-10 1.2 mm 25 x 75 10 Gross 430.00 Add to Cart
71870-20 1.2 mm 25 x 75 20 Gross 750.00 Add to Cart

arrow10Microscope Slide, Sandblasted, Frosted End

These slides have a 3/4″ sandblasted, frosted end.

25 x 75 mm x 1 mm (T)

Catalog # Thickness Size,mm Pack Price
71877-19 1 mm 25 x 75 Gross 43.00 Add to Cart
71877-20 1 mm 25 x 75 10 Gross 398.00 Add to Cart

Microscope Slide, SUPERFROST® Endarrow13Microscope Slide, SUPERFROST® End

With a superfrosted end. These slides have one side which is frosted with a brilliant white opaque coating that will take and retain all pen markings. They offer superior readability and they are permanent.

Catalog # Thickness Size,mm Pack Price
71867-01 1 mm 25 x 75 Gross 37.75 Add to Cart
71867-10 1 mm 25 x 75 10 Gross 342.00 Add to Cart
71868-01 1.2 mm 25 x 75 Gross 47.00 Add to Cart
71868-10 1.2 mm 25 x 75 10 Gross 420.00 Add to Cart

Microscope Slide, SUPERFROST® PLUS - Adhesion Slidearrow13SUPERFROST® PLUS – Adhesion Slide

These Superfrost Plus(+) glass slides are made by a new process which offers the following features

  • They place a permanent positive charge in the microscope slide
  • They electrostatically attract frozen tissue sections and cytology preparations, binding them to the slide
  • They form a bridge so that covalent bonds develop between formalin fixed sections and the glass
  • Tissue sections and cytological preparations adhere better to the Plus glass slides without the need for special adhesives or protein coatings

Benefits

  • Plus glass puts an end to background staining
  • It eliminates blue or red background staining which is often encountered in hematoxylin and eosin staining of tissue sections mounted on albuminized glass
  • No brown background staining occurs when immuno-peroxidase or in situ DNA procedures are performed on our Plus glass. A higher degree of enzyme digestion can be performed on plus glass without the loss of tissue sections
  • Cytological preparations placed on Plus glass resist cell loss during enzyme digestion, DNA denaturation and RNA hybridization
  • Plus glass improves tissue adherence in microwave staining
  • Chromic acid washes are redundant for molecular hybridization because the slides are RNAse-free
  • Plus slides are Color coded to help keep track of specimens during processing as well as filing

The slides measure: 25 x 75 mm, 1 mm thick.

71869-10 Superfrost Plus Slide, White 1 Gross 83.00 Add to Cart
71869-11 Superfrost Plus Slide, White 10 Gross 795.00 Add to Cart
71869-20 Superfrost Plus Slide, Blue 1 Gross 85.00 Add to Cart
71869-21 Superfrost Plus Slide, Blue 10 Gross 830.00 Add to Cart
71869-30 Superfrost Plus Slide, Green 1 Gross 85.00 Add to Cart
71869-31 Superfrost Plus Slide, Green 10 Gross 830.00 Add to Cart
71869-40 Superfrost Plus Slide, Pink 1 Gross 85.00 Add to Cart
71869-41 Superfrost Plus Slide, Pink 10 Gross 830.00 Add to Cart
71869-50 Superfrost Plus Slide, Yellow 1 Gross 85.00 Add to Cart
71869-51 Superfrost Plus Slide, Yellow 10 Gross 830.00 Add to Cart

technical dataSuperfrost® Plus Gold Slidesarrow13SUPERFROST® PLUS Gold Slides

These slides are specially designed for the application of fresh or formalin fixed frozen tissue. They are inherently tissue binding so there is no need to add sprays or adhesives to the slides.

Features

  • Securely attach frozen breast, brain, skin and other normally difficult to bond tissue sections to the slide
  • Ideal when special stains, immunocytochemical and in situ DNA hybridization techniques are applied on fresh and fixed frozen tissue
  • Assist tissue adherence during immunocytochemical analysis of estrogen and progesterone receptors in fresh frozen breast tissue
  • They are compatible with both toluidine blue and hematoxylin and eosin rapid frozen section stains
71864-01 Superfrost Plus Gold Slides 25/bx 38.00 Add to Cart
71864-10 Superfrost Plus Gold Slides 10bx/cs (250) 360.00 Add to Cart

Colormark™ Plus Glass Slidearrow13Colormark™ Plus Glass Slide

Specimen Slide identification is VITAL. Automated etched slide labeling instruments ensure specimen slide integrity but are difficult to read on ordinary slides. To clearly read diamond scribed slide labels against any surface, you need the only slide designed to be used with your automated slide labeling instrument. You need COLORMARK slides.These brightly printed slides greatly improve etched labeling visibility and offer all the advantages of Superfrost¨ slides. A bright contrasting color has been printed on the back of the Superfrost end so that patient or specimen information etched through the painted end on the front is clearly visible. Colormark Plus slides are adhesive coated therefore ensuring the adhesion of the section to the slide during staining procedures.

63400-10 White Frosted End Front, Black frosted End Back 1 gross 82.00 Add to Cart
63400-11 White Frosted End Front, Black frosted End Back 10 gross 775.00 Add to Cart
63400-20 Blue Frosted End Front, Blue Frosted End Back 1 gross 83.00 Add to Cart
63400-21 Blue Frosted End Front, Blue Frosted End Back 10 gross 778.00 Add to Cart
63400-30 Green Frosted End Front, Green Frosted End Back 1 gross 84.00 Add to Cart
63400-31 Green Frosted End Front, Green Frosted End Back 10 gross 798.00 Add to Cart
63400-40 Pink Frosted End Front, Red Frosted End Back 1 gross 85.00 Add to Cart
63400-41 Pink Frosted End Front, Red Frosted End Back 10 gross 805.00 Add to Cart
63400-50 Lavender Frosted End Front, Red Frosted End Back 1 gross 84.00 Add to Cart
63400-51 Lavender Frosted End Front, Red Frosted End Back 10 gross 798.00 Add to Cart
63400-60 Lavender Frosted End Front, Black Frosted End Back 1 gross 84.00 Add to Cart
63400-61 Lavender Frosted End Front, Black Frosted End Back 10 gross 798.00 Add to Cart

arrow13Control Slides

Use our Histology Control Slides and you will always know for sure which specimen is the Patients and which is the Control.

Features

  • Patient and Control tissue samples on the same slide
  • Positive identification of control and patient samples
  • Proof of correct staining procedures
  • Utmost adhesion of samples to slide throughout staining procedure
  • Easy and permanent identification of slides

Available in two configurations: Style #329+ with ¾ inch of frosted area; style 334+ with 1 inch frosted area.

63448-10 Control Slide 329+ 144/pk 78.00 Add to Cart
63448-20 Control Slide 334+ 144/pk 82.00 Add to Cart

Cytology Slidearrow13Special Glass Slides

arrow13Cytology Slide: 1 x 3″ (25 x 75 mm), 1 mm Thick

Superfrost letters VCE and lines on the same side as the Superfrost end.

71874-01 Cyto-Slide Letters VCE & Lines 1 gross 42.00 Add to Cart
71874-10 Cyto-Slide Letters VCE & Lines 10 gross 395.00 Add to Cart

Superfrost letters VC and lines on the same side as the Superfrost end.

71875-01 Cyto-Slide Letters VC & Lines 1 gross 42.00 Add to Cart
71875-10 Cyto-Slide Letters VC & Lines 10 gross 395.00 Add to Cart

71876-01arrow13Full-Frosted Cytology Microscope Slides

Full frosted cytology microscope slides. 75 mm (L) x 25 mm (W), 1 mm thick.

  • Surface frosting over one entire side of slide.
  • Frosting disappears when coverslip is mounted.
  • Does not interfere with viewing.
71876-01 Full-Frosted Cytology Slide 144/pk 48.00 Add to Cart
71876-10 Full-Frosted Cytology Slide 10/pk/case 430.00 Add to Cart

arrow13Round Corner Microslides

71887-152 Round Corner Microslides 144/pk 38.00 Add to Cart

Fluorescent Antibody Slides with Etched Ringsarrow13Slides with Etched Rings

Useful for Fluorescent Antibody Examination. The size of the slide is 3″ x 1″, approximately 1 mm thick, with an etched frosted end. Pre-cleaned. Packed 72/box.

71879-10 Two Etched 10 mm Circle Slide Gross 40.00 Add to Cart
71879-11 Two Etched 10 mm Circle Slide 10 Gross 385.00 Add to Cart
71879-15 Two Etched 15 mm Circle Slide Gross 40.25 Add to Cart
71879-16 Two Etched 15 mm Circle Slide 10 Gross 392.00 Add to Cart

uperFrost™ Clipped Corner Slidearrow13SuperFrost™ Clipped Corner Slide

Designed specially for the Sysmex SP-100 slide prep unit and recommended for the Diagnostics. The 45 degree clipped corner slide offers exceptional cleanliness and resistance to sticking. The tight tolerances for the length and width help to reduce accumulation of glass chips within the instrument. The pre-cleaned slide is 25 x 75 mm and approximately 1 mm thick. Ideal for technicians, who routinely prepare large number of slides.

71882-01 SuperFrost™ Clipped Corner Slide 1 gross 45.00 Add to Cart
71882-10 SuperFrost™ Clipped Corner Slide 10 gross 430.00 Add to Cart

Large Glass Slidesarrow13Large Glass Slides

In addition to our comprehensive collection of glass slides manufactured by ESCO we now carry large glass slides which are available in 75 x 38 mm and 75 x 51 mm. These slides are precleaned, individually selected and are suitable for large specimen sections, such as, brain tissue.

71860-01 75 x 38 mm Glass Slide, 1 mm Thick Gross 58.00 Add to Cart
71862-01 75 x 51 mm Glass Slide, 1.2 mm Thick Gross 80.00 Add to Cart

arrow13Glass Slides

Our square microscope glass slides are made from clear, white, pre-cleaned glass. They are moisture, dirt, and film free. All of our slides are flat, consistent, and stand up to repetitive use. They are made from the highest quality glass sheets. The slides’ dimensions meet U.S. Government standards. They are carefully packed to assure cleanliness and the glass is fully annealed to minimize chipping. Glass thickness is 1 mm.

71881-10 Glass Slide, 25 x 25 mm, Ground Edges 36/pk 72.00 Add to Cart
71881-20 Glass Slide, 76 x 100 mm, Plain Cut Edges 36/pk 81.00 Add to Cart

arrow13ClariTex Slides and Coverslips IFU

These ready-to-use slides are manufactured from super grade glass with uniform thickness, fulfilling optical requirements for light microscopy.

71887-201arrow13ClariTex Plain Slides

Edges are ground with 90° corners. Available in packs of 1000.

  • Plain slide for routine use.
  • Pre-cleaned and ready to use.
  • Dimensions: 26.0 (W) x (76.0 (D) mm.
71887-201 ClariTex Microscope Slides, Plain 1000/pk 60.00 Add to Cart

71887-202arrow13ClariTex Singlefrost Slides

These slides have a 20 mm frosted end on one side. Edges are ground with 90° corners. Available in packs of 1000.

  • Frosted section at one end.
  • Pre-cleaned and ready for use.
  • Dimensions: 26.0 (W) x 76.0 (D) mm.
71887-202 ClariTex Singlefrost Microscope Slides 1000/pk 48.00 Add to Cart

71887-203arrow13ClariTex Twinfrost Slides

These slides have a 20 mm frosted end on both sides. Edges are ground with 90° corners. Available in packs of 1000.

  • Frosted section on both sides.
  • Pre-cleaned and ready for use.
  • Dimensions: 26.0 (W) x 76.0 (D) mm.
71887-203 ClariTex Twinfrost Microscope Slides 1000/pk 58.00 Add to Cart

arrow13ClariTex ColourCoat Slides

These slides are recommended for labeling or marking with a pencil or pen. Their 20 mm frosted area is resistant to all routine chemicals and stains used in laboratories. WhiteCoat slides are available with 45° clipped corners.

71887-20471887-20671887-20771887-20871887-20971887-21071887-211

  • 20 mm silky color frosted area.
  • Available in six colors.
  • Pre-cleaned and ready to use.
  • Dimensions: 26.0 mm (w) x 76.0 mm (l).
  • Thickness: 1.1 ± 0.1 mm.
71887-204 ClariTex WhiteCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-205 ClariTex WhiteCoat Slides, Clipped Corner 1000/pk 80.00 Add to Cart
71887-206 ClariTex GreenCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-207 ClariTex BlueCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-208 ClariTex OrangeCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-209 ClariTex PinkCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-210 ClariTex YellowCoat Microscope Slides 1000/pk 77.00 Add to Cart
71887-211 ClariTex PurpleCoat Microscope Slides 1000/pk 77.00 Add to Cart

71887-212arrow13ClariTex Supa Mega Plain Slides

These double-width slides allow sections to be much larger. Pathologists can spend less time switching between slides that hold smaller sections. Edges are ground with 90° corners. Available in packs of 100.

  • Pre-cleaned and ready to use.
  • Dimensions: 52.0 (W) x 76.0 (D).
71887-212 ClariTex Supa Mega Plain Microscope Slides 100/pk 39.00 Add to Cart

72887-213arrow13ClariTex Supa Mega Twinfrost Slides

These double-width slides have a 10 mm frosted end on both sides. They can be marked with a pencil or pen. Edges are ground with 90° corners. Available in packs of 100.

  • Pre-cleaned and ready to use.
  • Dimensions: 52.0 (W) x 76.0 (D) mm.
71887-213 ClariTex Supa Mega Twinfrost Microscope Slides 100/pk 40.00 Add to Cart

arrow13ClariTex Coverslips

ClariTex Coverslips are for in vitro diagnostic applications. They ensure that specimens are secure and protected. Their flat, smooth surface will maintain optical quality even when archived for several years.

  • Manufactured from green soda-lime glass.
  • Uniform size and flatness.
  • Sealed in aluminum tropical packs.

ClariTex Coverslips No. 1.0 Thickness

71887-214 24 x 50 mm 1000/pk 58.00 Add to Cart
71887-215 22 x 50 mm 1000/pk 54.00 Add to Cart
71887-216 22 x 22 mm 1000/pk 21.00 Add to Cart
71887-217 Supa Mega, 50 x 64 mm 100/pk 33.00 Add to Cart

ClariTex Coverslips No. 1.5 Thickness

71887-218 24 x 50 mm 1000/pk 40.00 Add to Cart
71887-219 22 x 50 mm 1000/pk 21.00 Add to Cart
71887-220 22 x 22 mm 1000/pk 20.00 Add to Cart
71887-221 Supa Mega, 50 x 64 mm 100/pk 30.00 Add to Cart

Microscope Slide – Gold Sealarrow13Microscope Slide – Gold Seal®

We proudly introduce GOLD SEAL® microscope slides for your convenience.

GOLDSEAL® slides are made from pure white glass (sodalime) and at accurately cut sizes. To preserve the highest quality from manufacturer to ultimate user, all GOLD SEAL® slides are hermetically sealed in our Moisture-Barrier™ system, providing a dry-slide environments during shipment and storage.

UltraStick™ (adhesion coating: APTES, 3-Aminopropyl Triethoxysilane) – Adhesion coated micro slides- one of the best from GOLD SEAL® are ideal for histological and cytological applications demanding superior specimen-to-slide adhesion. The enhanced silane coating eliminates tedious procedures and special additives to your water bath.

Procedures such as immunoperoxidase, in situ DNA hybridization, enzyme digestion and frozen tissue processing will benefit from the amplified adhesion characteristics of this product. Overall cost savings and assured quality of your prepared specimen will be recognized with UltraStick™ from Gold Seal®.

arrow13Plain Slides
Catalog # Gold Seal Description Pack Price
63710-05 3011 3 x 1″, 1 mm Thick 1 gross 43.00 Add to Cart
63710-25 3011 3 x 1″, 1 mm Thick 25 gross 830.00 Add to Cart
63711-05 3048 3 x 1″, 1.2 mm Thick 1 gross 45.00 Add to Cart
63711-25 3048 3 x 1″, 1.2 mm Thick 25 gross 860.00 Add to Cart
63712-05 3010-P 3 x 1″, 1 mm Thick – Cytologentics processing 1 gross 43.00 Add to Cart
63712-25 3010-P 3 x 1″, 1 mm Thick – Cytologentics processing 25 gross 850.00 Add to Cart
arrow13Patented Moisture-Barrier™ enclosing 5 gross inner packages
Catalog # Gold Seal Description Pack Price
63718-05 3054 25 x 75 mm, 1 mm Thick, Plain 5 gross 236.00 Add to Cart
63718-25 3054 25 x 75 mm, 1 mm Thick, Plain 25 gross 875.00 Add to Cart
63719-05 3055 25 x 75 mm, 1 mm Thick, White Rite-On™ Frost 5 gross 310.00 Add to Cart
63719-25 3055 25 x 75 mm, 1 mm Thick, White Rite-On™ Frost 25 gross 1,215.00 Add to Cart
arrow13Rite-On™ Frosted Slides with brilliant white epoxy ink
Catalog # Gold Seal Description Pack Price
63721-05 3050 25 x 75 mm, 1 mm Thick, Frosted One End, One Side 1 gross 55.00 Add to Cart
63721-25 *** 25 x 75 mm, 1 mm Thick, Frosted One End, One Side 25 gross 1,075.00 Add to Cart
63722-05 3050-P 25 x 75 mm, 1 mm Thick, Frosted End, One Side, Cytogentics Processing 1 gross 55.00 Add to Cart
63722-25 3050-P 25 x 75 mm, 1 mm Thick, Frosted End, One Side, Cytogentics Processing 25 gross 1,075.00 Add to Cart
63723-05 3049 3 x 1″, 1.2 mm Thick, Frosted One End, One Side 1 gross 64.00 Add to Cart
63723-25 3049 3 x 1″, 1.2 mm Thick, Frosted One End, One Side 25 gross 1,350.00 Add to Cart

***Gold Seal equivalent product offered

arrow13UltraFrost™ Frosted Slides, white epoxy ink frosted one end, one side
Catalog # Gold Seal Description Pack Price
63728-05 3063 25 x 75 mm, 1 mm Thick, Frosted one End with Gold Seal Logo 1 gross 58.00 Add to Cart
63728-25 3063 25 x 75 mm, 1 mm Thick, Frosted one End with Gold Seal Logo 25 gross 1,090.00 Add to Cart
63729-05 3064 25 x 75 mm, 1 mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal® Logo 1 gross 60.00 Add to Cart
63729-25 3064 25 x 75 mm, 1 mm Thick, Frosted one End with Pre-Print Patient Information Label and Gold Seal® Logo 25 gross 1,245.00 Add to Cart
arrow13Fluorescent Antibody Slides
Catalog # Gold Seal Description Pack Price
63732-05 3032 3 x 1″, 1.2 mm Thick with Two 10 mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 1 gross 84.00 Add to Cart
63732-02 3032 3 x 1″, 1.2 mm Thick with Two 10 mm diameter circles, Frosted one Side, one End with White Epoxy Ink, including Circles 25 gross 1,720.00 Add to Cart
arrow13UltraStick™/UltraFrost™ Adhesion Slides frosted one end, one side with epoxy ink
Catalog # Gold Seal Description Pack Price
63734-01 3039 25 x 75 mm, 1 mm Thick, Surface Coated with APTES for Adhesion 1 gross 73.00 Add to Cart
63734-10 3039 25 x 75 mm, 1 mm Thick, Surface Coated with APTES for Adhesion 10 gross 690.00 Add to Cart

technical dataQuartz Microscope Slides and Coverslipsarrow13Quartz Microscope Slides and Coverslips

There are many applications that require slides and coverslips that have absolute UV transparency, and high temperature properties. EMS now is introducing the highest quality, clear fused quartz microscope slides and coverslips to meet all of these requirements.

  • The window of highest transparency to UV is between 0.15µ to 5µ
  • Typical flatness measured 2 to 4″ bands per inch
  • Soften point is about 1250°C (2282°F)
  • Standard microscope slide thickness is 1 mm
  • Standard coverslip thickness is 0.2 mm
arrow13Quartz Microscope Slides
72250-01 Slide 1″ x 1″ (25 x 25 mm), 1 mm thick each 14.00 Add to Cart
72250-02 Slide 2″ x 1″ (50 x 25 mm), 1 mm thick each 21.50 Add to Cart
72250-03 Slide 3″ x 1″ (75 x 25 mm), 1 mm thick each 24.00 Add to Cart
72250-04 Slide 2″ x 3″ (50 x 76 mm), 1 mm thick each 75.00 Add to Cart
72250-05 Slide 10 x 10 mm, 1 mm thick each 16.00 Add to Cart

These slides have all of the same properties as the ones above; the only difference is these are 0.5 mm thick.

72250-06 Slide 1″ x 1″ (25 x 25 mm), 0.5 mm thick each 28.00 Add to Cart
72250-07 Slide 2″ x 1″ (50 x 25 mm), 0.5 mm thick each 32.00 Add to Cart
72250-08 Slide 3″ x 1″ (75 x 25 mm), 0.5 mm thick each 34.00 Add to Cart
arrow13Quartz Coverslips
72255-02 Coverslip Rd 1″ (25 mm), dia. each 29.00 Add to Cart
72256-02 Coverslip Sq 1″ x 1″ (25 x 25 mm) each 29.00 Add to Cart
72256-03 Coverslip Rd 18 mm (0.71″) x 0.2 mm Thick (#2) each 35.00 Add to Cart
72256-04 Coverslip Rd 25.4 mm (1″) x 0.2 mm Thick (#2) each 29.00 Add to Cart
72256-05 Coverslip Rd 33.02 mm (13.0″) x 0.2 mm thick (#2) each 39.00 Add to Cart
72256-06 Coverslip Rd 25 mm (0.984″) x 0.15-0.18 mm thick (#1) each 46.00 Add to Cart
72256-07 Coverslip Sq 25.4 mm (1″) x 0.2 mm thick (#2) thick each 46.00 Add to Cart
72256-08 Coverslip Sq 25.4 mm (1″) x 0.15-0.18 mm thick (#1) each 62.00 Add to Cart

Permanox™ and Polystyrene Microscope Slidesarrow13Permanox™ and Polystyrene Microscope Slides

Permanox™ is a strong, biologically inert material resistant to non-chlorinated hydrocarbons, acetone, and other EM reagents. Permanox™slides allow you to do cell fixation and staining on the slide; they can be cut with scissors. Permanox™ has not been shown to be autofluorescent. All of the microscope slides are 1″ x 3″.

NOTE: Long exposure to solvents will cause the slide to warp. Xylene and Toluene based mounting media should be avoided. Glycerol, gelatin, and other aqueous based media are recommended.

71885 Permanox™ Microscope Slide 20/pk 80.00 Add to Cart
71886 Permanox™ Microscope Slide 100/pk 338.00 Add to Cart
71888 Polystyrene Microscope Slide 20/pk 76.00 Add to Cart
71889 Polystyrene Microscope Slide 100/pk 311.00 Add to Cart

Disposable Rinzl Plastic Micro Slidesarrow11Disposable Rinzl Plastic Micro Slides

  • Made from optical clear vinyl with a refractive index of glass
  • Ideal for wet mount, urines, etc
  • Economical, they can be used and discarded
  • Available standard size 3 x 1″ and packaged 144 per box
  • Will not break, scratch, or cut fingers
  • Not auto-fluorescent
  • Thickness 0.0180 to 0.022″ or 0.46 to 0.56 mm
71890-12 Rinzl Plastic Micro Slides (A) 12/box 4.25 Add to Cart
71890-01 Rinzl Plastic Micro Slides (B) 144/box 13.00 Add to Cart
71890-10 Rinzl Plastic Micro Slides (B) 10 x 144/box 105.00 Add to Cart

Poly-L-Lysine Coated and Silane Treated Microscope Slidearrow13Poly-L-Lysine

Our premium glass slides with a frosted end are now available pre-coated with either Poly-L-Lysine or Silane (aminoalkysilane). These slides improve the adhesion of tissues to the slide.

63410-01 Poly-L-Lysine Coated Slides 72/bx 56.00 Add to Cart
63410-02 Poly-L-Lysine Coated Slides 144/bx 101.00 Add to Cart
63411-01 Silane Coated Slides 72/bx 56.00 Add to Cart
63411-02 Silane Coated Slides 144/bx 101.00 Add to Cart

Technical Tip

Poly-L-Lysine Coating of Slides

Polysine™ Microscope Adhesion Slidearrow13Polysine™ Microscope Adhesion Slide

The permanent bio-adhesive material on our Polysine™ slide attracts frozen and paraffin embedded sections, centrifuge preparation and cytology smears.

63412-01 Polysine™ Adhesion Slide 72/bx 60.00 Add to Cart
63412-02 Polysine™ Adhesion Slide 2 x 72/bx 105.00 Add to Cart

Excell™ Adhesion Slidesarrow13Excell™ Adhesion Slides

The Excell slide was developed with superior adhesion and wettability properties, and has been shown to perform very well in most applications

  • Excell™ Adhesion Surface for Heat Induced Epitope Retrieval – Excell™ was developed for use in the HIER methods that require high pH antigen retrieval solutions, including EDTA. Formalin fixed paraffin embedded tissue demonstrated more consistent attachment when performing these procedures on the Excell™ surface
  • Automated Staining – Excell™ has a uniform hydrophilic surface that works extremely well with automated staining equipment
  • Cell Culture – Excell™ surface technology conditions the surface to enhance the attachment of anchorage dependent cell lines, including many fastidious lines, such as primary neuronal cells. Attachment and proliferation is increased so cells achieve a confluent monolayer sooner
  • Plastic – Excell™ works well for both Epon and Methyl Methacrylate sections. Its uniformly hydrophilic surface allows the sections to spread well during initial attachment. Section adherence is maintained throughout lengthy staining procedure
  • Laser Capture Microdissection – Excell™ gives you the advantage of excellent tissue adherence of all tissue types, yet it easily releases those area of interest identified for Laser Capture Microdissection.
64370-01 Excell™ Adhesion Slide 144/pk 112.00 Add to Cart

arrow13Adhesion Slide – TOMO®

These TOMO hydrophilic adhesion slides offer superior tissue adhesion, staining performance and slide surface fluidics. Proprietary slide coating ensures consistent performance even after exposure to heat stress and humid environmental conditions. Optimized for use with thermal printers.

These slides are recommended for use on Ventana® Benchmark staining platform and automated IHC platforms.

These slides are exceptionally flat, with low fluorescence glass, and finished with 90° and clipped corners.

The slides are packaged 100 per box, 10 boxes per case (1000 slides) and they measure 3″ x 1″ (75 x 25 mm).

Catalog# Description Qty. Price
63705-01 TOMO Adhesion Slide, White, 90° 100/pk 42.00 Add to Cart
63705-02 TOMO Adhesion Slide, White, 90° 1000/cs 390.00 Add to Cart
63705-03 TOMO Adhesion Slide, White, 45° 100/pk 42.00 Add to Cart
63705-04 TOMO Adhesion Slide, White, 45° 1000/cs 390.00 Add to Cart
63705-05 TOMO Adhesion Slide, Blue, 45° 100/pk 42.00 Add to Cart
63705-06 TOMO Adhesion Slide, Blue, 45° 1000/cs 390.00 Add to Cart
63705-07 TOMO Adhesion Slide, Green, 45° 100/pk 42.00 Add to Cart
63705-08 TOMO Adhesion Slide, Green, 45° 1000/cs 390.00 Add to Cart
63705-09 TOMO Adhesion Slide, Pink, 45° 100/pk 42.00 Add to Cart
63705-10 TOMO Adhesion Slide, Pink, 45° 1000/cs 390.00 Add to Cart
63705-11 TOMO Adhesion Slide, Yellow, 45° 100/pk 42.00 Add to Cart
63705-12 TOMO Adhesion Slide, Yellow, 45° 1000/cs 390.00 Add to Cart

Text Box:arrow13Adhesion Slide – TruBond™

Adhesive slides with proven performance under high temperature and pH conditions

These adhesive slides, including the hydrophilic TruBond™ 360, work well with automatic IHC applications. EMS is confident that the quality of these slides will exceed your expectations. For problems with tissue loss try our TruBond™ 380.

Available colors: White, Blue, Green and Yellow.

 

TruBond™ 380 TruBond™ 360
MAS-GP Adhesion Slides
TruBond™ 200
APS Adhesion Slide
Glass Type High Clarity, Low
Fluorescence White
High Clarity, Low Fluorescence White High Clarity, Low Fluorescence White
Surface Wettability Hydrophilic Hydrophilic Hydrophobic
Adhesion Very Strong Strong Strong
Edge Treatment Ground Corner Ground Corner Ground Corner
For Manual IHC Yes Yes Yes
For Automatic IHC Yes Yes Yes
For Fluorescence Yes Yes Yes
Recommended Applications – HIER processes when tissue type require extra strong adhesion
– Epitope enhancement & DNA probe procedures
– Autopsy & brain sectioning
– All IHC applications
– HIER pH 6 & pH 9 protocols
– Routine histology
– Frozen section
– All IHC applications
– Routine histology

Ordering

TruBond™ is packaged 100 slides per box, 10 boxes to a case.

Item# Description Color 100 slides/bx 10 boxes/cs
63700-Wx TruBond™ 380 White 40.00 Add to Cart 352.00 Add to Cart
63700-Bx TruBond™ 380 Blue 40.00 Add to Cart 352.00 Add to Cart
63700-Gx TruBond™ 380 Green 40.00 Add to Cart 352.00 Add to Cart
63700-Yx TruBond™ 380 Yellow 40.00 Add to Cart 352.00 Add to Cart
63701-Wx TruBond™ 360 White 40.00 Add to Cart 352.00 Add to Cart
63701-Bx TruBond™ 360 Blue 40.00 Add to Cart 352.00 Add to Cart
63701-Gx TruBond™ 360 Green 40.00 Add to Cart 352.00 Add to Cart
63701-Yx TruBond™ 360 Yellow 40.00 Add to Cart 352.00 Add to Cart
63702-Wx TruBond™ 200 White 40.00 Add to Cart 352.00 Add to Cart
63702-Bx TruBond™ 200 Blue 40.00 Add to Cart 352.00 Add to Cart
63702-Gx TruBond™ 200 Green 40.00 Add to Cart 352.00 Add to Cart
63702-Yx TruBond™ 200 Yellow 40.00 Add to Cart 352.00 Add to Cart
63703-Bx TruView™ Fine Blue 20.00 Add to Cart 104.00 Add to Cart
63703-Gx TruView™ Fine Green 20.00 Add to Cart 104.00 Add to Cart
63703-Yx TruView™ Fine Yellow 20.00 Add to Cart 104.00 Add to Cart

For Trubond Coverslips – click here

arrow13LiveSlide® Living Slides

LiveSlide
LiveSlide

These innovative, optically clear microscope slides have proven to unmask rarely seen features of microorganisms.

A shallow reservoir confines them to a liquid depth not much larger than the depth of field of the microscope, yet deep enough to allow the microscopic animals to move about freely.

View more videos

LiveSlide® Features

  • Uniform depth of large viewing well is 0.009″
  • Large viewing area has partitions to keep specimens in viewing area
  • Optically clear
  • Made of Makrolon® 2558 polycarbonate
  • Includes two reusable coverslips made of Makrofol®, 0.005″ and 0.010″
  • Excellent impact resistance for safety indoors and outdoors
  • Both the base and the coverslips are reusable
  • Can be used as a traditional slide simply by flipping the base over
  • Can be used with traditional and digital microscopes
  • 100% made in the USA
71877-01 LiveSlide each 3.75 Add to Cart
71877-05 LiveSlides 5/pk 17.50 Add to Cart
71877-10 LiveSlides 10/pk 34.00 Add to Cart
71877-50 LiveSlides 50/pk 160.00 Add to Cart

BrightSlide Darrow12BrightSlide™ D

Fluorescence Enhancing Slides and Versatile, (bio)-functionalized coatings for Glass, Metal Oxides and ITO

Fluorescence systems (e.g. microscopes) are today widely used in the life science research. For many applications an increased sensitivity compared to existing glass slides is required. The BrightSlide™ D technology uses a proprietary dielectric coating to significantly enhance the signal intensity of fluorescence molecules from the surface. It can be read out with all systems that illuminate and detect from the top. The technology makes use of purely optical principles and substitutes conventional glass substrates.

The BrightSlide™ D (Dielectric) comes standard as a bare SiO2 Surface.

BrightSlide D
Enhancement of the fluorescence on BrightSlide™ image of 150nm thick section of biological materialBrightSlide D
Fluorescence signal on standard, glass slide image of 150nm thick section of biological material

Benefits

  • Better signal to noise ratio
  • Less sample material can be used
  • More genes can be detected
  • Existing instrument platform can be used
  • Purely optical amplification of fluorescence signal
  • Dielectric, high quality SiO2 surface ready to apply customer bio-functional coating (use your own bio-functional coating, no change of chemical protocol required)

Applications

  • Universal detection platform for fluorescence detection on planar surfaces, e.g. fluorescence detection of ultra thin samples
  • Differential gene expression using Cy3 and Cy5 labels simultaneously
  • Gene expression using Cy3 or Cy5 labels independently

Specifications

Optimization 488 nm and 590 nm excitation wavelengths (other wavelengths on request)
Polarization Both s- and p-polarizations can be used for excitation and emission
Excitation Light 0° – 3° Angle of Incidence
Background Ultralow Fluorescence
Label Durable, chemically resistant with a unique serial number
Compatibility Suitable for most of commercially available fluorescence microscopes with illumination and detection from the top
Substrate Material D263T eco (other materials on request)
Substrate Format 75 ±0.05 mm x 25 ±0.05 mm x 1.1 ±0.1 mm (other formats on request)
Active Area slide: 63 mm x 22 mm
optical coating: 5/2 x 0.4; C2 x 0.4; E1.0 according to DIN ISO 10110
Chemical Composition (top surface) Clean, dielectric, SiO2 surface (ITO coating on request)
Environmental Stability (optical coating only) Stable against temperatures up to 200°C. All strongly oxidizing cleaning protocols can be used. All organic solvents can be used. Chemical reagents which etch SiO2(HF, NaOH, KOH) can destroy the enhancement effect of the BrightSlide™
Storage Store in clean environment at room temperature

Ordering Information

63426-D BrightSlide™ D each. 400.00 Add to Cart
63426-D-10 BrightSlide™ D 10/pk 3,900.00 Add to Cart
63426-D-100 BrightSlide™ D 100/pk 24,000.00 Add to Cart

Borosilicate Capillary Micro Glass Slidearrow12Borosilicate Capillary Micro Glass Slide

Made from Corning® 7740 borosilicate, low expansion, type I glass.

Micro glass slides are precision rectangular glass capillary tubes for use in microscopic viewing of liquid samples. Capillary action instantly collects the sample and the thin glass walls minimize evaporation. The design of these cells eliminates the needs for cover glass and the hanging drop method. These micro glass slides can withstand heat up to 500°C. Samples can be retained for future reference by sealing or cementing open ends. Micro glass slide are packaged in plastic vials.

I.D. tolerance ± 10%, Wall tolerance ± 20%

Catalog# Dimensions ID mm
Path x width
Wall
Thickness
Length mm Qty. Price
63820-05 0.02 x 0.2 0.020 50 30/vial 40.00 Add to Cart
63820-10 0.02 x 0.2 0.020 100 30/vial 64.00 Add to Cart
63821-05 0.03 x 0.3 0.025 50 33/vial 43.00 Add to Cart
63821-10 0.03 x 0.3 0.025 100 33/vial 75.00 Add to Cart
63822-05 0.04 x 0.4 0.030 50 36/vial 44.00 Add to Cart
63822-10 0.04 x 0.4 0.030 100 36/vial 78.00 Add to Cart
63823-05 0.05 x 0.5 0.040 50 44/vial 62.00 Add to Cart
63823-10 0.05 x 0.5 0.040 100 44/vial 108.00 Add to Cart
63824-05 0.10 x 1.0 0.090 50 40/vial 53.00 Add to Cart
63824-10 0.10 x 1.0 0.090 100 40/vial 92.00 Add to Cart
63825-05 0.20 x 2.0 0.150 50 36/vial 49.00 Add to Cart
63825-10 0.20 x 2.0 0.150 100 36/vial 88.00 Add to Cart
63826-05 0.30 x 3.0 0.300 50 31/vial 43.00 Add to Cart
63826-10 0.30 x 3.0 0.300 100 31/vial 71.00 Add to Cart
63827-05 0.40 x 4.0 0.400 50 28/vial 62.00 Add to Cart
63827-10 0.40 x 4.0 0.400 100 28/vial 96.00 Add to Cart
63828-10 0.50 x 5.0 0.300 100 14/vial 68.00 Add to Cart
63829-10 0.60 x 6.0 0.400 100 12/vial 57.00 Add to Cart
63830-10 0.70 x 7.0 0.460 100 9/vial 44.00 Add to Cart
63831-10 0.80 x 8.0 0.540 100 7/vial 59.00 Add to Cart
63832-10 0.90 x 9.0 0.600 100 6/vial 47.00 Add to Cart
63833-10 1.00 x 10.0 0.670 100 4/vial 56.00 Add to Cart
63840-05 0.05 x 1.0 0.030 50 50/vial 59.00 Add to Cart
63840-10 0.05 x 1.0 0.030 100 50/vial 114.00 Add to Cart
63841-05 0.10 x 2.0 0.100 50 45/vial 62.00 Add to Cart
63841-10 0.10 x 2.0 0.100 100 45/vial 102.00 Add to Cart
63842-05 0.20 x 4.0 0.150 50 36/vial 52.00 Add to Cart
63842-10 0.20 x 4.0 0.150 100 36/vial 85.00 Add to Cart
63843-05 0.30 x 6.0 0.300 50 24/vial 44.00 Add to Cart
63843-10 0.30 x 6.0 0.300 100 24/vial 79.00 Add to Cart
63844-05 0.40 x 8.0 0.400 50 18/vial 44.00 Add to Cart
63844-10 0.40 x 8.0 0.400 100 18/vial 79.00 Add to Cart

twin adhesive well stickers (1cm diameter) firmly adhere to microscope slidesarrow13Microscope Slide-Wells

Disposable Liquid-Holding Wells

Convenient Pre-cut, twin adhesive well stickers (1 cm diameter) firmly adhere to microscope slides. Examine specimens with or without cover slips. Available in depths of 0.25 mm and 0.50 mm.

Multiple ApplicationsExamine or count tissue culture cells, yeast, bacteria and other microorganisms. Piggy-back with Microscope Slide-Grids to facilitate counting and sizing.

Versatile – Compatible with different styles of microscopes (conventional, inverted or dissecting).

Chemically Inert – Made of polyester with an acrylic adhesive. Microscope Slide-Wells are inert and stable under most laboratory conditions.

70366-16 Slide Wells, 0.25 mm thick 25/pk 44.00 Add to Cart
70366-13 Slide Wells, 0.50 mm thick 25/pk 56.00 Add to Cart

Disposable Adhesive Gridsarrow13Microscope Slide-Grids

Disposable Adhesive Grids

Convenient – Easy-to adhere stickers having side-by-side twin grids (20 mm x 20 mm each with 1 mm line spacing) for microscope slides. Count or measure cells and estimate the size or length of microscopic or semi-micro objects

Versatile – Compatible with different styles of microscopes (conventional, inverted or dissecting). Virtually all cells and specimens placed on top of Slide-Grids can be counted and/or sized.

Dry Or Wet – Use with dry specimens or suspended specimens such as cells held in buffer under cover slips. Separate cover slips can be placed over each Slide-Grid.

Chemically Inert – Made of polyester with an acrylic adhesive. Slide-Grids are inert and stable under most laboratory conditions.

70366-14 Slide Grids, 20 mm x 20 mm 40/pk 30.00 Add to Cart

Quick-Read Microscope Slides with Gridsarrow13Quick-Read™ Microscope Slide with Grid

These slides are especially manufactured to cater to the Microscopic Urinalysis testing. They are made from clear acrylic with 10 chambers, each chamber has 81 pre-measured wells that hold a determined volume of urinary sediment, giving fast and convenient microscopic examination of each specimen.

64633-01 Quick-Read Microscope Slide with Grids 100/pk 160.00 Add to Cart

Quick-Read Microscope Slidesarrow13Quick-Read™ Microscope Slide

These slides are especially manufactured to cater to the Microscopic Urinalysis testing. They are made from clear acrylic with 10 chambers, each chamber has 18 pre-measured circles that hold a determined volume of urinary sediment, giving convenient and easy microscopic examination of cellular elements in 1 ml of urine. The circles are able to be seen as a group at 100x magnification.

64633-02 Quick-Read Microscope Slide 100/pk 160.00 Add to Cart

Gridded Microscope Slidesarrow13Gridded Microscope Slides

These 3 x 1″ slides have an indexed grid on the reverse side of the frosted end. The ink is resistant to stains and common laboratory solvents. Each square grid fills one low-powered-field (approximately 100x magnification). These slides make it easy to locate an organism or cell every time. There are sixty-four squares in total. The slides are available with coatings of Poly-L-Lysine or they can be purchased plain (uncoated).

The grid measures 2 mm square-16 mm total. Numbers 1-8 and alpha A-H.

arrow131. Gridded Microscope Slides without hydrophobic coating.
63405-01 Grid Slides, Uncoated 72/pk 75.00 Add to Cart
63405-02 Grid Slides, Uncoated 144/pk 139.00 Add to Cart
63406-01 Grid Slides, Poly-L-Lysine 72/pk 82.00 Add to Cart
63406-02 Grid Slides, Poly-L-Lysine 144/pk 147.00 Add to Cart
arrow132. Gridded Microscope Slides with hydrophobic coating.
63408-01 Grid Slides, Round well 72/pk 98.00 Add to Cart
63409-02 Grid Slides, Square well 72/pk 98.00 Add to Cart

arrow13Cardboard “Well” Slides

EMS introduces these unique cardboard “well” slides. Complete with glass covered slide and aluminum holder, these slides are less prone to static as compared to similar plastic slides.

arrow131. Microscope slides made from cardboard housed in aluminum holders complete with glass coverslips.

Slides measure 76 mm (L) x 27.2 mm (W) x 4.5 mm (H)

Cardboard Slide
63515-01
Cardboard Slide
63515-02
Cardboard Slide
63515-03
Cardboard Slide
63515-04
Cardboard Slide
63515-05
Cardboard Slide
63515-06
Cardboard Slide
63515-07
Cardboard Slide
63515-08
Cardboard Slide
63515-09
Cardboard Slide
63515-10
Cardboard Slide
63515-11
Cardboard Slide
63515-12
arrow132. Double depth slides with paper top and acetate or glass cover slip.

Slides measure 74.7 mm (L) x 25.3 mm (W) x 3.6 mm (H)

Cardboard Slide
63515-13
Cardboard Slide
63515-14

Specifications

Cat. # Number of Wells Space/Well Dimensions Color
63515-01 1 Well, 64 Numbered Spaces 2.5 x 4.5 mm Black
63515-02 1 Well, 64 Numbered Spaces 2.5 x 4.5 mm White
63515-03 1 Well, 32 Numbered Spaces 4 x 4 mm Black
63515-04 1 Well, 32 Numbered Spaces 4 x 4 mm White
63515-05 10 Wells 5 mm dia. Black
63515-06 10 Wells 5 mm dia. White
63515-07 4 Wells 12 mm dia. Black
63515-08 4 Wells 12 mm dia. White
63515-09 2 Wells 15 mm dia. Black
63515-10 2 Wells 15 mm dia. White
63515-11 1 Well 15 mm dia. Black
63515-12 1 Well 15 mm dia. White
63515-13 1 Double Depth Well 15 mm dia. Black
63515-14 1 Double Depth Well 15 mm dia. White

Ordering Information

63515-01 Slide in Holder w/Glass Coverslip, 64 Spaces, Black 25/pk 90.00 Add to Cart
63515-02 Slide in Holder w/Glass Coverslip, 64 Spaces, White 25/pk 90.00 Add to Cart
63515-03 Slide in Holder w/Glass Coverslip, 32 Spaces, Black 25/pk 90.00 Add to Cart
63515-04 Slide in Holder w/Glass Coverslip, 32 Spaces, White 25/pk 90.00 Add to Cart
63515-05 Slide in Holder w/Glass Coverslip, 10 Wells, Black 25/pk 90.00 Add to Cart
63515-06 Slide in Holder w/Glass Coverslip, 10 Wells, White 25/pk 90.00 Add to Cart
63515-07 Slide in Holder w/Glass Coverslip, 4 Wells, Black 25/pk 90.00 Add to Cart
63515-08 Slide in Holder w/Glass Coverslip, 4 Wells, White 25/pk 90.00 Add to Cart
63515-09 Slide in Holder w/Glass Coverslip, 2 Wells, Black 25/pk 90.00 Add to Cart
63515-10 Slide in Holder w/Glass Coverslip, 2 Wells, White 25/pk 90.00 Add to Cart
63515-11 Slide in Holder w/Glass Coverslip, 1 Well, Black 25/pk 90.00 Add to Cart
63515-12 Slide in Holder w/Glass Coverslip, 1 Well, White 25/pk 90.00 Add to Cart
63515-13 Slide, Paper Top w/ Coverslip, 1 Double Depth Well, Black 25/pk 90.00 Add to Cart
63515-14 Slide, Paper Top w/ Coverslip, 1 Double Depth Well, White 25/pk 90.00 Add to Cart

PTFE Printed Slidesarrow13

PTFE Printed Slides

PTFE Printed Slides, are extremely hydrophobic, acetone proof, chemical resistant and autoclavable. They are perfect for controlling cross-contamination in staining, immunolabeling and immunofluorescence tests. All printed slides are precleaned. The well surface is 100% wettable, which enhances cell attachment and increases its spreading capabilities within the well. These slides minimize the use of expensive purified antigens and antibodies. The slides measure 3 x 1″ (76 x 26 x 1 mm).

EMS Printed Slides are manufactured to order offering maximum shelf life. The shelf life of PTFE Printed Slides range from 18 months to two years. However, it is recommended that all glass slides (Printed or un-printed) be used in a timely manner to avoid glass “weathering”, (slides becoming cloudy or hazy even with proper storage. All of our Printed Slides are autoclavable and have a frosted end. They are intended for one time use only.

Features

  • Super Hydrophobic Mask
  • Clean, Wettable Wells
  • A Bioadhesive Surface Treatment to Enhance Cell and Tissue Attachment
Catalog No. Number of Well Well Size Pack Price
63414-13 1 Ring 13 mm Dia. 72 69.00 Add to Cart
63414-10 Square 10.7 x 10.7 mm 72 62.00 Add to Cart
63415-12 1 Round 12 mm Dia. 72 89.00 Add to Cart
63415-32 1 Oval 24.4 x 16.7 mm 72 72.00 Add to Cart
63415-08 1 Round 8 mm Dia. 100/bx 96.00 Add to Cart
63416-08 2 Round 8 mm Dia. 72 96.00 Add to Cart
63416-15 2 Round 15 mm Dia. 72 82.00 Add to Cart
63417-13 2 Ring 13 mm Dia. 72 79.00 Add to Cart
63418-11 3 Ring 11.28 mm Dia. 72 79.00 Add to Cart
63419-08 3 Round 8 mm Dia. 72 97.00 Add to Cart
63419-12 3 Round 12 mm Dia. 72 79.00 Add to Cart
63419-14 3 Round 14 mm Dia. 72 79.00 Add to Cart
63419-VIR 3 Round TGH VIROLOGY 72 79.00 Add to Cart
63420-05 4 Round 5 mm Dia. 72 79.00 Add to Cart
63420-08 4 Round 8 mm Dia. 72 79.00 Add to Cart
63420-11 4 Ring 11.43 mm Dia. 72 79.00 Add to Cart
63421-07 5 Round 7 mm Dia. 72 79.00 Add to Cart
63421-10 6 Round 10 mm Dia. 72 79.00 Add to Cart
63421-11 Etched 5 Round 11.43 mm Dia. 72 79.00 Add to Cart
63423-05 6 Round 5 mm Dia 72 79.00 Add to Cart
63423-08 6 Round 8 mm Dia. 72 79.00 Add to Cart
63422-06 8 Round 6 mm Dia. 72 79.00 Add to Cart
63422-11 8 Round 11 mm Dia. 72 79.00 Add to Cart
63424-06 10 Round 6 mm Dia. 72 79.00 Add to Cart
63425-05 12 Round 5 mm Dia. 72 79.00 Add to Cart
63429-04 21 Round 4 mm Dia. 72 79.00 Add to Cart
63430-04 24 Round 4 mm Dia. 72 79.00 Add to Cart
63434-02 30 Round 2 mm Dia. 72 79.00 Add to Cart

PTFE Printed Slidesarrow13

PTFE Printed Slides

PTFE Printed Slides which are extremely hydrophobic and acetone proof, chemically resistant and autoclavable. The slides measure 3×1″ (76 x 26 x 1 mm), 72 slides per pack.

PTFE Printed Slides

Features

  • Super Hydrophobic Blue Mask
  • Clean Wettable Wells
Catalog Glass Size, Configuration Well/Ring
size mm
Price
63412-15 3″ x 1″, 2 ring, blue 15 90.00 Add to Cart

Macro/Latex Agglutinationarrow13

Macro/Latex Agglutination

25 to a pack

Catalog Glass Size, Configuration Well/Ring
size mm
Price
63450-31 4″ x 3″, 3 ring, yellow 26.4 198.00 Add to Cart
63450-32 4.25″ x 2″, 3 ring, white 26.4 96.00 Add to Cart
63453-61 4.4″ x 2.1″, 6, white oval 17.4 x 26 202.00 Add to Cart
63453-63 4.215″ x 2″, 6 white oval 17.4 x 26 202.00 Add to Cart
63456-15 4.331″ x 2.165″, 10 ring white 15 99.00 Add to Cart
63459-15 4.75″ x 3.5″, 15 ring white 15 202.00 Add to Cart
63460-15 3″x 2″ x 1.2 mm, 15 well 11.28 etched 202.00 Add to Cart

Lovins Micro-Slide Field Finderarrow13

Lovins Micro-Slide Field Finder

Marking a microscope slide to identify a particular field of interest for future reference has always been a difficult problem for many microscopists.

The Field Finder has a precision rectangular-coordinate grid pattern, which is accurately positioned on a special microscope slide. The slide has been carefully edge ground to provide straight edges and square corners to ensure repeatable results.

Lovins Micros-Slide Field Finder helps you to index points of interest on a microscope slide the same way you find them on a road map. A standard 75 x 25 mm glass slide has a photo etched grid pattern of 1 mm squares, subdivided into 0.1 mm intervals. Each square is marked with a letter and a number. You center the detail in the field of the microscope, replace the specimen slide with the field finder, and read the coordinates. Note reading on slide, and you can find that detail immediately by reversing procedure.

The pattern is imaged in vacuum deposited chrome for durability.

72266-01 Micro-Slide Field Finder, 75 x 25 mm each 95.00 Add to Cart

arrow13Retriever Tissue Slides

Retriever Tissue Slides are high adhesive glass microscope slides for paraffin-embedded tissue section, including tissue arrays. The slides were designed and optimized for the EMS Antigen Retriever (62700 series) to preserve the section attachment and tissue morphology during heat-induced epitope recovery in Retriever in pH 6 (Low), pH 9 (High), or Universal Retriever buffer.

Slides can also be successfully used in any other epitope-recovery technology (autoclave, microwave, proteolitic) and show superior performance over positively charged or polylysine-coated slides, especially in high pH buffers and recovery buffers containing EDTA.

These slides are ideal for both IHC and immunofluorescent applications, as well as for all antigen recovery systems.

Each of the slides have a custom imprinted frosted colored end, each color representing a different glass surface.

General Properties

  • Retriever Tissue Slides are made from soda lime glass
  • Dimensions approximately 76 x 26 mm, Thickness 1.0 mm (± 0.05 mm)
  • 90° ground edges
  • Colored or white marking area 20 mm, at one end, on one side
  • Pre-cleaned, ready for use
  • Autoclavable
  • Supplied in plastic boxes of 100 pieces
  • Certified for in-vitro-diagnostic (IVD) application

Specifications

71880-50 HistoBond® + F, Blue Both surfaces of these slides bond tissue sections adhesively with their positive charge. No additional adhesives are required. The reliable anchoring prevents tissue sections to float during washing and cooking. Reliable for routine IHC with FF/PE sections that involve antigen retrieval.
71880-50-CS HistoBond® + F, Blue
71890-50 HistoBond® + R, Orange Superior type of adhesion slide with a considerably higher positive charge than other regular charged slides. This results in strong adhesion of the tissue, even under alkaline conditions of EDTA/EGTA-containing buffers. IHC, IF, in-situ, hybridization, grade slides.
71890-50-CS HistoBond® + R, Orange
71890-51 HistoBond® + X, White Tissue sections anchor covalently on the glass surface. Even a non-polar tissue, e.g. very fatty (breast, brain, etc.) that does not cause a charge difference between the adhesive layer and the section, reliably bonds chemically to the glass.
71890-51-CS HistoBond® + X, White

Ordering

71880-50 Retriever Tissue Slides, HistoBond® + F, Blue 100/pk 90.00
71880-50-CS Retriever Tissue Slides, HistoBond® + F, Blue 20 pk/cs 1,350.00
71890-50 Retriever Tissue Slides, HistoBond® + R, Orange 100/pk 60.00
71890-50-CS Retriever Tissue Slides, HistoBond® + R, Orange 20 pk/cs 1,011.00
71890-51 Retriever Tissue Slides, HistoBond® + X, White 100/pk 64.00
71890-51-CS Retriever Tissue Slides, HistoBond® + X, White 20 pk/cs 1,081.00 Add to Cart

arrow13Petrographic Microscope Slides

Petrographic Slides

Glass slides made from fine white glass, with ground and polished edges. 72 slides to a box.

71534-01 Petrographic Microscope Slides, 46 x 27 x 1.2 mm 72/pk 60.00 Add to Cart

For Petrographic Slide Boxes, click here.