AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1

AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1
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AppliChem A2132 Aprotinin BioChemica CAS 9087-70-1

Physical Description:  Solid

Product Code: A2132

Product Name:  Aprotinin BioChemica

Headline Comment:

® registered trademark of Bayer AG

Specifications:

Assay (protein): min. 90 %
Activity (KIU/mg): min. 5500
pH (1 %; H2O): 5.0 – 7.5
Water (K.F.): max. 6 %

Comment:

Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.Stability\: Aprotinin may be stored indefinite at \+4°C in lyophilized form. Dissolved in saline or buffer solutions (e. g. 10 mg/ml) at pH 5 – 8, it may be stored aliquoted at \+4°C at least for one month or frozen at -20°C for several years. Thawed aliquots of the stock solutions should not be frozen again, because repeated freeze-thawing will lead to aggregation of the polypeptide aprotinin. Solutions with a pH value below 4 or above 9 should be used immediately. Protect from direct sunlight, UV light and reducing agents. It is stable in water, 70 % methanol, 70 % ethanol or 50 % acetone, too (2, 3).Unit-Definition: One Trypsin Inhibitor Unit (TIU) will decrease the activity of 2 trypsin units by 50 %, where one trypsin unit will hydrolyze 1.0 μmole Nα-benzoyl-DL-arginine p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (Trypsin Inhibitor Unit) corresponds to approx. 1300 KIU (Kallikrein Inhibitor Units) and 1 TIU corresponds to one Ph. Eur. Unit.

Bibliography:

(1)Kassell, B. & Laskowski, Sr., M. (1965) Biochem. Biophys. Res. Com. 20, 463-468.The basic Trypsin inhibitor of bovine pancreas\: V. The disulfide linkages. (2)Kassell, B. (1970) Methods Enzymol. 19, 844-852Bovine Trypsin-Kallikrein inhibitor (Kunitz Inhibitor, Basic Pancreatic Trypsin Inhibitor, Polyvalent Inhibitor from Bovine Organs). (3)Fritz, H. & Wunderer, G. (1983) Drug Res. 33, 479-494Biochemistry and application of Aprotinin, the Kallikrein inhibitor from bovine organs.

Storage:  2-8°C

产品名称:Aprotinin BioChemica
规格:10mg
货号:A2132_0010
厂商:AppliChem
产品介绍:
Storage: 2-8°C
LGK: 10 – 13
Formula C284H432N84O79S7
M 6511.52 g/mol
CAS-No.: 9087-70-1
HS-No.: 35040090
origin from bovine lung
Specification
Assay (Protein) min. 90 %
Activity (KIU/mg) min. 5500
pH (1 %; H2O) 5.0 – 7.5
Water (K.F.) max. 6 %
Aprotinin was isolated independently from two laboratories and originally named Bovine Pancreatic Trypsin Inhibitor (BPTI) and Trypsin-kallikrein Inhibitor (TKI). It is a strongly basic protein (58 amino acids; ref. 1, 2). Aprotinin inhibits trypsin, chymotrypsin, kallikrein from different sources and plasmin. Its recommended working concentration is 2 – 10 μg/ml (approx. 1 μM). The working concentration given in ref. 3 (500 KIU/ml; approx. 10 μM) seems to be very high.
AppliChem  A2132_0010 Aprotinin BioChemica 10mg 543
AppliChem  A2132_0025 Aprotinin BioChemica 25mg   1117
AppliChem  A2132_0100 Aprotinin BioChemica 100mg  3936

AppliChem Myco系列支原体污染清除试剂

AppliChem Myco系列支原体污染清除试剂

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Myco-1|A5222_0010 AppliChem

产品名称:Myco-1
规格:10ml
货号:A5222_0010
厂商:AppliChem
产品介绍:
Storage: -20°C
Shipment: wet ice
Synonym Tiamulin
usage sterile solution
HS-No.: 38220000
for the treatment of Mycoplasma-infected cells.
Application in combination with Myco-2 (A5233).
100X concentrated antibiotic solution.
based on the antibiotic Tiamulin
AppliChem A5222_0010 Myco-1 10ml 929
AppliChem A5222_0020 Myco-1 20ml 1614

Myco-2|A5233_0010 AppliChem

产品名称:Myco-2
规格:10ml
货号:A5233_0010
厂商:AppliChem
产品介绍:
Synonym Minocycline
usage sterile solution
HS-No.: 38220000
Storage: -20°C
Shipment: wet ice
for the treatment of Mycoplasma-infected cells.
Application in combination with Myco-1 (A5222).
100X concentrated antibiotic solution.
based on the antibiotic Minocycline
The contamination of cells with mycoplasma is a very common problem, even though it often goes unnoticed since no cloudiness appears in the cell culture. Nevertheless the contamination often causes biochemical changes as well as changes in the immunological properties of the cells. Since mycoplasma-infected cells cannot always be discarded, many complicated methods have been suggested for the elimination of the mycoplasma. AppliChem offers a combination of antibiotics, which have been shown to be effective in the elimination of mycoplasma species that account for 90 % of the contamination found in cell cultures.
AppliChem A5233_0010 Myco-2 10ml 731
AppliChem A5233_0020 Myco-2 20ml 1104

Myco-3|A5240_0010 AppliChem

产品名称:Myco-3
规格:10ml
货号:A5240_0010
厂商:AppliChem
产品介绍:
Lagerung: -20°C
HS-Nr.: 38220000
für die Behandlung Mycoplasmen-infizierter Zellen.
Anwendung ohne zusätzliche Antibiotika.
100X konzentrierte Antibiotika-Lösung.
basiert auf dem Antibiotikum Ciprofloxacin
AppliChem A5240_0010 Myco-3 10ml 514
AppliChem A5240_0020 Myco-3 20ml 929
AppliChem A5240_0100 Myco-3 100ml 3737

德国 Applichem A7089 DNA-ExitusPlus 核酸污染清除试剂

德国 Applichem A7089 DNA-ExitusPlus 核酸污染清除试剂

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Product Code:  A7089

Product Name: DNA-ExitusPlus™

Specifications:

  • removes DNA and RNA contaminations from surfaces
    • for cleaning of PCR work stations and equipment
    • for cleaning of electrophoresis equipment, pipetts, reaction tubes etc.
    • delivered as spray bottles or as refill bottles (RF)
    ♦ The special features
    • non-enzymatic degradation of DNA and RNA by catalytic and cooperative effects of the components
    • All components of DNA-ExitusPlus™ are readily biologically degradable and not harmful or toxic for humans.
    • Doesn’t contain aggressive mineralic acids or alkaline substances
    • No toxic fumes. Contains low concentration of alcohol.
    Functionality (Strand breakage test): passes test
    Efficacy (ExitusPlus™ activity test): passes test

Comment:

DNA amplification is one of the most commonly used techniques in the modern research laboratory. The presence of contaminating DNA in and around PCR workstations can lead to unwanted artifacts during amplification. Principally, there are two ways to make DNA not amplifiable:1. by degradation of the DNA (e. g. by the addition of DNases or chemical destruction), or2. by modification of bases – leaving the DNA strand intact, but blocked for reading by polymerases. Using a DNA strand break assay (designed by multiBIND biotec GmbH, Germany), it has been shown that not all DNA decontamination solutions on the market totally degrade DNA! DNA-ExitusPlus™ is an improvement over those products and causes both strand breakages as well as degradation. When used properly at the work area, it totally eliminates the amplification of non-target DNA. DNA-ExitusPlus™ is a non-alkaline, non-corrosive and non-carcinogenic cleansing solution which is effective on all surfaces.A severe disadvantage of conventional decontamination reagents is revealed in a new test for the corrosive potential of their components. For this purpose, different metal plates were incubated for 20 minutes with identical aliquots of the reagents. The selected metals are representative for equipment and materials found in laboratories. The result of this corrosion test show that all known commercial products contain aggressive chemical substances with corrosive, harmful or even toxic effects. These conventional reagents are known to contain azides, mineralic acids like phosphoric acid or hydrochloric acid, aggressive peroxides or strong alkaline substances like sodium hydroxide. Even after an incubation of only 20 minutes, irreversible damages of the metal surfaces are observed in many cases. The newly developed solution DNA-ExitusPlus™ exhibits its unique characteristics especially in this corrosion test. For all metal surfaces tested, no damage or corrosion is observed after treatment. DNA-ExitusPlus™ was also tested under identical conditions on many different plastic surfaces without any indications of damage.DNA-ExitusPlus™ is a registered trademark of AppliChem GmbH.

Bibliography:

(1)Esser, K.-H. et al. (2006) Nature Methods 3(2), 151DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents.

Storage: RT   protected from light

Nature Methods推荐PCR污染清除方法——德国PanReac AppliChem DNA-ExitusPlus
货号Cat.No.: A7089,0100(100ml)     A7089,0500(500ml)      A7089,1000(1L)

聚合酶链式反应(Polymerase Chain Reaction)简称PCR,是一种选择性体外扩增核酸片段的方法,经过循环复制,扩增产量是指数上升,所以具有极高的灵敏度,被广泛应用于生物和医学的科研,临床各种研究中,是目前应用最为广泛的分子生物学方法之一。
但也正是由于PCR具有极高的灵敏度,微量的核酸污染便会导致假阳性扩增结果而导致实验结果不准确,这也是广大实验者最头痛的问题,如果形成气溶胶污染扩散,则可引起整个PCR实验室的污染,处理起来非常棘手,严重的甚至要关闭实验室。环境中的核酸是主要的污染源,常见的污染源有:
1. 模板提取时真空抽干装置
2. 超净台
3. 电泳装置
4. 紫外分析仪
5. 剪刀、镊子、刀片、移液器等器械
6. 离心机
7. 冰箱门把手,冷冻架,门把手或实验台面等
8. 气溶胶
9. 高压灭菌装置

传统的PCR污染清除方法主要有:稀酸处理法、紫外照射法、尿嘧啶糖苷酶(UNG)法、酶解变性、酒精沉淀等几种方式,但仍然存在无法彻底破坏DNA分子、试剂具有腐蚀性或者毒性、小片段核酸无法清除等不足。

德国AppliChem独家研发的DNA-ExitusPlus是一种无腐蚀、无致癌性的核酸污染清除溶液,该产品可将裸露的核酸完全降解至无法作为扩增模板的小片段,无法被扩增,且无法恢复,从而彻底消除了PCR过程中由于环境污染而导致的假阳性扩增。

DNA-ExitusPlusTM特点:
1. 产品为非酶类试剂;
2. 产品中各组分协同作用,快速、非序列特异性的降解核酸污染;
3. 所有组分可生物降解、无毒无害;
4. 不含有机溶剂或者挥发性物质,不含无机酸或者碱性物质,不会对金属形成腐蚀;
5. 使用简单,喷洒或浸泡处理15分钟即可完成清除作用;
6. 升温至50度可提高反应速度。

AppliChem A5219二氧化碳培养箱水源消毒剂 Incuwater-Clean

AppliChem A5219二氧化碳培养箱水源消毒剂 Incuwater-Clean

AppliChem品牌试剂代理-上海金畔生物科技有限公司
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AppliChem A5219二氧化碳培养箱水源消毒剂 Incuwater-Clean

Product Code: A5219

Product Name:  Incuwater-Clean™

Headline Comment:

  • Incuwater-Clean™ is a registered trademark of AppliChem GmbH.

Specifications:

  • Disinfectant solution for CO2-incubator water baths.
    • 100X concentrated solution. Use 50 ml per 5 liter of incubator water bath.
    • for the prevention of microbial growth in incubator water baths.

Comment:

The water required to create the humidity is a source of contamination which disperses in the incubator. In order to disinfect the water we recommend Incuwater-Clean™ solution, which contains a disinfectant that does not cause damage to the stainless steel tray, is non-toxic, non-volatile, and extremely effective. The water should be replaced with sterile water every two to four weeks, adding 50 ml of Incuwater-Clean™ per 5 liters of water. This product can be applied in normal water baths too.

Storage: 2-8°C

【详细说明】

实验室里,细胞培养接触水源是支原体,细菌,真菌和病毒zui容易传播和交叉污染的地方,也是实验室消毒zui难处理的地方,包括细胞培养箱产生的气雾、培养箱内专用水源以及水浴锅等设备,易污染、难清除、危害大等问题是威胁细胞正常生长和表达的重要因素。

来自德国素有”小Sigma”之称的AppliChem,独创Myco支原体清除系列,能有效的清除细胞培养过程中的支原体污染,为广大科研工作者解决了困扰许久的难题,同时,也推出系列预防产品,以防止失而复得的细胞再次遭受污染之灾。德国AppliChem独创专门针对水池抗支原体污染的系列产品。产品应用于容器表面的消毒, 以及二氧化碳培养箱蓄水槽, 各种水箱, 及清洗用水池的消毒。

Incuwater-Clean 主要用于细胞培养箱内的水源消毒,为100X浓缩液,直接加入灭菌水中,使用方便,该试剂高效无毒,无挥发性,并且不会腐蚀金属表面。建议培养箱中每2-4周更换一次灭菌水,并加入Incuwater-Clean。

订购信息:

品牌 产品货号 品名 规格  
AppliChem A5219 Incuwater-Clean 100ml  

另外还有用于CO2培养箱消毒剂Incubator-Clean喷雾消毒剂和水浴锅消毒剂Aquabator-Clean,消毒彻底,安全无毒,使用方便,这一系列预防污染产品全方位为您的细胞保驾护航。

AppliChem A1117 BCIP BioChemica CAS:6578-06-9

AppliChem A1117 BCIP BioChemica CAS:6578-06-9

AppliChem品牌试剂代理-上海金畔生物科技有限公司
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Code: A1117
CAS :6578-06-9
Molecular Formula : C8H6BrClNO4P · C7H9N
Molar mass :433.64 g/mol
中文名称:5-溴-4-氯-3-吲哚磷酸
英文名称:BCIP BioChemica
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
厂商:AppliChem
产品介绍:
Storage: -20°C  protected from light
Solubility (20°C) 20 mg/ml (DMF)
Formula C8H6BrClNO4P · C7H9N
M 433.64 g/mol
CAS-No.: 6578-06-9
HS-No.: 29339980
EC-No.: 229-506-1
Specification
λmax. (buffer pH 7.0) 286 – 294 nm
E 1 %,1 cm, λmax. min. 5800 (pH 7.0)
Water (K.F.) max. 1 %
Synonym 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, X-Phos p-toluidine salt, X-Phos-pT
AppliChem A1117_0001 BCIP BioChemica 1g 1929
AppliChem A1117_0005 BCIP BioChemica 5g 7515
AppliChem A1117_0250 BCIP BioChemica 250mg 660
AppliChem A1117_0500 BCIP BioChemica 500mg 1119

德国AppliChem A3778 DNase I 脱氧核糖–上海金畔生物代理

德国AppliChem A3778 DNase I 脱氧核糖–上海金畔生物代理

AppliChem品牌试剂代理-上海金畔生物科技有限公司
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DNase I,即Deoxyribonuclease I,中文名称为脱氧核糖核酸酶I,是一种可以消化单链或双链DNA产生单脱氧核苷酸或单链或双链的寡脱氧核苷酸的核酸内切酶。DNase I水解单链或双链DNA后的产物,5’端为磷酸基团,3’端为羟基。
DNase I活性依赖于钙离子,并能被镁离子或二价锰离子激活。镁离子存在条件下,DNase I可随机剪切双链DNA的任意位点;二价锰离子存在条件下,DNase I可在同一位点剪切DNA双链,形成平末端,或1-2个核苷酸突出的粘末端。

Physical Description:   Solid

Product Code:  A3778

Product Name:   DNase I

Short Description:   delivery form: salt-free, freeze-dried powder, chromatographically prepared

Specifications:

Activity (Kunitz): min. 3000 U/mg

Comment:

Deoxyribonuclease I (DNase I) from beef pancreas is an endonuclease (glycoprotein), which preferentially cleaves the phosphodiester bond in the DNA behind pyrimidine nucleotides. This results in a polynucleotide with a 5′-phosphate and a free OH-group in position 3′. DNase I cleaves single-stranded and double-stranded DNA as well as chromatin. The specificity of the enzyme reaction (single-strand-‘Nicks’ versus double-strand breakes) is determined by the ions available. In the presence of Mg2\+ single-strand nicks are generated and in the presence of Mn2\+ double-strand breakes. The pH-optimum of DNase I is 7.8 and it is activated by divalent cations. Maximum activation requires the presence of Mg2\+ and additional Ca2\+. Calcium ions (5 mM) protect DNase I from proteolytic digest. Inhibition is achieved by citrate, if activation is done by magnesium, but not if manganese has been the activator. Besides it is inhibited by chelators such as EDTA and SDS or β-mercaptoethanol.The enzyme is used in molecular biology techniques like digestion of DNA, in the RNA purification (ref. 2 Suppl. 1 pp. 4.1.4) or generating “random nicks” for “nick translation” (ref. 2 Suppl. 9 pp. 3.5.4-6) or ‘footprint’-assays (ref. 2 Suppl. 7 chapter 12.4) or investigations on chromatin (ref. 2 Suppl. 48 chapter 21.4.1).Unit definition\: One unit is defined as that amount of enzyme which causes an increase of absorbance at 260 nm of 0.001 per minute at 25°C based on the method of Kunitz. DNase I is readily soluble in e. g. 0.15 M sodium chloride or in reaction buffer (e. g. 50 mM Tris · Cl, pH 7.5; 10 mM MgCl2 (single-strand ‘nicks’) and 10 mM MnCl2 (double-strand breakes), respecitvely; 50 μg/ml BSA; ref. 2 Suppl. 8 page 3.12.5). For storage dissolve DNase I in 50 % glycerol (w/v); 20 mM Tris · Cl, pH 7.5; 1 mM MgCl2. For stability reasons the concentrations should be at least 1 mg/ml (The maximum solubility is 10 %). This solution is stable for more than one year (ref. 2 Suppl. 8 page 3.12.5). The lyophilized form is stable for 2 – 5 years if kept at \+4°C. If a solution is protease-free, DNase I will not loose significant activity at pH 5 – 7 and 62°C for 5 hours. The enzyme may be heat-inactivated (10 minutes at 99°C).RNase-free DNase I: Dissolve DNase I at 1 mg/ml in 0.1 M iodoacetic acid plus 0.15 M sodium acetate at a final pH of 5.3. The solution is then heated 40 minutes at 55°C and cooled. Finally, 1 M CaCl2 is added to the solution to 5 mM. Store frozen in small aliquots (according to ref. 2 page 3.12.6 Supplement 8).

Bibliography:

(1)Sambrook, J. & Russell, D.W. (2001) Molecular Cloning\: A Laboratory Manual, 3rd Edition. pp. A4.40-42. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (2)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, N.Y. (3)McDonald, M.R. (1955) Methods Enzymol. 2, 437-447Deoxyribonucleases (4)Campbell, V.W. & Jackson, D.A. (1980) J. Biol. Chem. 255, 3726-3735The effect of bivalent cations on the mechanism of DNase I. (5)Meinkoth, J. & Wahl, G.M. (1987) Methods Enzymol. 152, 91-94Nick-Translation. (6)Cobianchi, F. & Wilson, S.H. (1987) Methods Enzymol. 152, 94-110Enzymes for modifying and labeling of DNA and RNA.

Storage: -20°C

Origin: from bovine pancreas

产品信息:

品牌 货号 品名 规格
AppliChem A3778,0050 DNase I 50mg
AppliChem A3778,0100 DNase I 100mg
AppliChem A3778,0500 DNase I 500mg
AppliChem A3778_0010 DNase I 10mg

保存温度:-20℃,室温运输。

应用:DNase I 是一种需二价阳离子的脱氧核糖核酸内切酶,可用于降解单链或双链DNA

用于无DNA污染的RNA的制备,逆转录及体外转录等实验。

产品信息:M=~31000g/mol;CAS-NO:9003-98-9

性状:冻干粉

配置方法:溶解在150 mM 5毫克/毫升生理盐水,工作液为无色透明状。

特点:不含RNase(RNase free),可以用于各种RNA样品的处理。
用途:制备不含DNA的RNA样品;RT-PCR反应前RNA样品中去除基因组DNA等可能的DNA污 染;体外T7, T3, SP6等RNA Polymerases催化的RNA转录后去除DNA模板; DNase I footprinting研究DNA-蛋白质相互作用;缺口平移(nick translatioin);产生DNA随机片断文库;细胞凋亡TUNEL检 测中部分剪切基因组DNA作为阳性对照。
来源:牛胰腺纯化得到。
分子量:约31kDa。
活性检测条件:40mM Tris-HCl(pH8.0),10mM MgSO4,1mM CaCl2,1μg of pBR322 DNA。
纯度:不含其它DNA内切酶和外切酶,不含RNA酶。
酶储存溶液:50mM Tris-acetate(pH7.5),10mM CaCl2,50%(v/v)glycerol。
Reaction Buffer(10X)100mM Tris-HCl(pH7.5 at 25℃),25mM MgCl2,1mM CaCl2
失活或抑制:加入EDTA至终浓度为2.5mM后,65℃加热10分钟可使DNase I失活。酚氯仿抽提也可以使DNase I失活。金属离子螯合剂,达到毫摩尔/升浓度的锌离子,0.1%的SDS,DTT、巯基乙醇等还原 剂,50-100mM以上盐浓度均对DNase I有显著抑制作用。

注意事项:
如需对酶进行稀释,可以用酶储存液(50mM Tris-acetate(pH 7.5),10mM CaCl2,50%(v/v)glycerol)进行稀释。
酶使用时宜存放在冰盒内或冰浴上,使用完毕后宜立即放置于-20℃保存。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

Applichem A3744 支原体PCR检测试剂盒 PCR Mycoplasma Test Kit

Applichem A3744 支原体PCR检测试剂盒 PCR Mycoplasma Test Kit

AppliChem品牌试剂代理-上海金畔生物科技有限公司
       上海金畔生物代理AppliChem品牌试剂,欢迎新老客户访问AppliChem官网或者咨询我们获取相关产品信息。
      德国艾普力(APPLICHEM)代理
      AppliChem一级代理

【简单介绍】
AppliChem推出的PCR Mycoplasma Kit(货号:A3744)是一个即用型支原体检测试剂盒,无需支原体培养,只需要从培养细胞液中取上清,离心后获得可能含有支原体的沉淀,用50ul Kit内提供的缓冲液重悬后,95℃热击3分钟后即可作为PCR模板,加入Kit内提供的PCR反应液,并配成50ul PCR反应体系后即可按照Protocal提供的PCR设置进行PCR反应(如左图所示)。同时该支原体检测Kit内提供阳性对照,可完成20次检测。

Product Code:  A3744

Product Name:  PCR Mycoplasma Test Kit

Specifications:

♦ Ready-to-use PCR Mix for the detection of mycoplasma in cell culture
♦ detects all of myoplasma species found in cell cultures
♦ for 20 tests
♦ Kit components
• Reaction mix
• Buffer solution
• Positive template control
• Internal control DNA template
• Internal control primers mix

Comment:

The PCR Mycoplasma Test Kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. Mycoplasma detection by the direct culture procedure is time-consuming and some mycoplasma species are difficult to cultivate. With PCR testing, results are obtained within a few hours, since the presence of contaminant mycoplasma can be easily detected simply by verifying the bands of amplified DNA fragments in electrophoresis. There is no need to prepare probes labeled with radioisotopes, or to calculate enzyme, dNTP’s or buffer concentrations. Instead, a ready-to-use, optimized PCR mix is supplied. The primer set allows detection of various mycoplasma species (M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, M. arthritidis, M. bovis, M. pneumoniae, M. pirum, M. capricolum) as well as Acholeplasma and Spiroplasma species, with high sensitivity and specificity.Principle\: rRNA gene sequences of prokaryotes, including mycoplasmas, are well conserved, whereas, the lengths and sequences of the spacer region in the rRNA operon (for example the region between 16S and 23S gene) differ from species to species. The detection procedure utilizing the PCR process with this primer set consists of1.) Amplification of a conserved and mycoplasma-specific 16S rRNA gene region using two primers.2.) Detection of the amplified fragment by agarose gel electrophoresis.This system does not allow the amplification of DNA originating from other sources, such as cultured cells or bacteria, which affect the detection result. Amplification of the gene sequence with PCR using this primer set enhances not only the sensitivity, but also the specificity of detection. Amplified products are then detected by agarose gel electrophoresis.

Bibliography:

(1)Rottem, S. & Barile, F.M. (1993) TIBTECH 11, 143-150

Storage:  -20°C
Avoid repeated changes in the reaction mix temperature
When in use, always keep the reaction mix on ice!

Shipment:   wet ice in Germany, dry ice to abroad

【详细说明】

针对支原体检测,有多种方案可供选择,包括直接培养法、DNA荧光染色法等。其实就支原体污染的检测,zui简单有效的方案不外乎是使用PCR方法检测。即以支原体16S-23S之间极为保守的rRNA序列设计引物,经由PCR反应扩增其16S rRNA片段。该法非常灵敏(e.g. 0.1-1.6 CFU/5 ul 样品) 。由于引物的特殊性,不会导致培养细胞或其他细菌的序列的假性扩增。方法便捷,只需不到一天时间,并且无需支原体或细胞的培养过程,避免了可能的污染。

供应AppliChem A3672 DMSO 二甲基亚砜

供应AppliChem A3672 DMSO 二甲基亚砜

AppliChem品牌试剂代理-上海金畔生物科技有限公司

上海金畔生物代理AppliChem品牌试剂,欢迎新老客户访问AppliChem官网或者咨询我们获取相关产品信息。

德国艾普力(APPLICHEM)代理

AppliChem一级代理

供应AppliChem A3672 DMSO 二甲基亚砜

供应AppliChem A3672 DMSO 二甲基亚砜

CAS:67-68-5

Molecular Formula :C2H6OS

Molar mass:78.13 g/mol

Product Code:  A3672

Product Name:   Dimethyl sulfoxide Cell culture grade

Specifications:

Pyrogen test: passes test
Assay (GC): min. 99.5 %
Free acid: max. 0.001 %
Non-volatile matter: max. 0.001 %
Total P: max. 0.00001 %
Total Si: max. 0.00002 %
Water (K.F.): max. 0.1 %
Ca: max. 0.0001 %
Cu: max. 0.00005 %
Fe: max. 0.0001 %
K: max. 0.00005 %
Mg: max. 0.00005 %
Na: max. 0.0002 %
Pb: max. 0.00002 %
Zn: max. 0.00002 %

Comment:

Freezing of cells for cell culture \:Dimethyl sulfoxide (DMSO) is widely used for the freezing of cells in cell culture instead of glycerol. DMSO is added to prevent the formation of ice crystals during the freezing process, otherwise cells would be destroyed. DMSO is commonly used at the concentration of 10%. Please note: DMSO is toxic to cells! Therefore it is advisable to use pre-chilled “freeze medium”. Usually, such freeze medium consists of standard culture medium supplemented with 10% DMSO. Some researchers use 90% serum supplemented with 10% DMSO as freeze medium, which enhances the viability in some cell types. Particular cell types cannot be transferred into liquid nitrogen directly, but are slowly frozen to -70°C in a refrigerator. For very slow cooling of these stocks, cells are frozen well insulated and subsequently transferred to liquid nitrogen. Thawing: For further cultivation thawing should be carried out as quickly as possible. After thawing we recommend to wash the cells in medium or to change the medium the day after plating.

Applichem支原体预防试剂–A9390 Aquabator-Clean

Applichem支原体预防–A9390 Aquabator-Clean

AppliChem品牌试剂代理-上海金畔生物科技有限公司
       上海金畔生物代理AppliChem品牌试剂,欢迎新老客户访问AppliChem官网或者咨询我们获取相关产品信息。
      德国艾普力(APPLICHEM)代理
      AppliChem一级代理

支原体感染发生后能改变细胞的DNA,RNA及蛋白表达,又不能通过可视法对其进行检测,而且它对细胞的生长率影响较小,不易引起注意。实验室里,细胞培养接触水源是支原体最容易传播和交叉污染的地方,也是实验室消毒最难处理的地方,这里包括细胞培养箱产生的气雾、培养箱内专用水源以及水浴锅等设备。德国的AppliChem公司独创了专业针对水源抗支原体污染的系列产品,用于容器表面的消毒, 以及二氧化碳培养箱蓄水槽, 各种水箱, 及清洗用水池的消毒,下面详细介绍一下细胞培养箱水源消毒剂—Aquabater-Clean(a9390)。

Aquabater-Clean可用于预防各种水浴锅中真菌、细菌污染,是一种新型的即用型的方便快捷100倍浓缩液,它的有效成分对人是安全的,当在推荐的浓度下使用时(使用时每升水中加10ml Aquabater-Clean即可)对人的皮肤不会引起任何的刺激,而且该试剂高效无毒,安全可降解,2-8°C避光储存。

Applichem支原体预防–A9390 Aquabator-Clean

Product Code:
A9390
Product Name:
Aquabator-Clean™ (100X)
Headline Comment:
• Aquabator-Clean™ is a registered trademark of AppliChem GmbH.
Specifications:
• Disinfectant solution for ordinary water baths (not for CO2-incubators!)
• for the prevention of microbial growth in water baths
• 100X concentrated solution (Use 10 ml per 1 liter of water.)
品牌 货号 品名 规格
AppliChem A9390 Aquabator-Clean  (100X) 250ml

AppliChem为我们的客户连续不断的提供了可靠的和最好的质量保证,产品都是经过国际ISO 9001认证的, 而且能够满足广大用户的满足和特殊要求。另外,AppliChem还提了供针对细胞培养过程中各种可能支原体的污染源进行消毒的产品,如CO2培养箱的喷雾消毒Incubator-Clean™ (A5230)—一种即用型培养箱喷雾消毒剂,能有效预防真菌(及孢子)、细菌(及杆菌孢子)、支原体以及病毒污染(包括 HIV和乙肝病毒),它的有效成分是一组混合液,安全高效,不包含甲醛、苯酚等有害物质,是生物可降解的,建议每一周喷一次;培养箱内的水源消毒溶液Incuwater-Clean™ (A5219)—用于细胞培养箱内的水源消毒,该支原体预防试剂为100X浓缩液,直接稀释进水源使用,能有效杀灭并预防水源内的支原体污染,该试剂高效无毒,无挥发性,并且不会腐蚀金属表面,使用时用无菌水稀释100倍,每2-4周更换新液。

德国艾普力 AppliChem A0585 胰酶Pancreatin

德国艾普力 AppliChem A0585 胰酶Pancreatin

品  牌:德国艾普力AppliChem
产品名称:Pancreatin
规格:100g
货号:A0585_0100
厂商:AppliChem
产品介绍:
Lagerung: 2-8°C
LGK: 10 – 13
CAS-No.: 8049-47-6
HS-No.: 35079090
EG-No.: 232-468-9
Hinweis
Konversion der Einheiten: 6000 FIP-Einheiten der Lipase entsprechen 1X USP-Einheit. Das gelieferte Produkt entspricht somit ca. 4X USP-Einheiten.