VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection)
AK-6000
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Description
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Description
The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
Features:
Uses biotin/streptavidin technology
Can detect as little as 1 pg target protein when used with DuoLuX Substrate
Standard Kit contains Reagent A and Reagent B only
Sufficient reagents to develop twenty 100 cm2 blots
Specifications
More Information
Unit Size
1 kit
Applications
Blotting Applications, Elispot
Technology
ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s)
Alkaline Phosphatase
Target Species
No antibody included
Format
Concentrate
Kit Components
Kit Contents:
0.5 ml Reagent A and 0.5 ml Reagent B
Documents
User Guide
Troubleshooting Guide for VECTASTAIN ABC-AmP Kits
Safety Data Sheet
Download CoA
Datasheet
Related Products
ImmPACT® Vector® Red Substrate, Alkaline Phosphatase (AP) Catalog #: SK-5105
Vector® Black Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5200
Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300
Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.
Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits for Western Blot Detection VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection)
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Description
The VECTASTAIN ABC Reagent can be used to detect any biotinylated molecule. This is the original avidin/biotin ABC complex formation with biotinylated anti-rat IgG secondary antibody.
Features:
Good sensitivity
Low background
Kit contains sufficient reagents to stain approximately 1000-2000 sections
We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.
Should the VECTASTAIN ABC kit only be stored at 4 degrees C? Can it be stored at -20 degrees C?
We do not recommend freezing the ABC kits. In particular, the ABC reagents exhibit a decrease in enzyme activity and lose some sensitivity when stored at – 20 °C for extended periods.
With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
Kit Contents:
3 ml normal rabbit serum
1 ml biotinylated rabbit anti-rat IgG secondary antibody
2 ml each of concentrated A and B reagents
Product review on Biocompare.com.
The Avidin Biotin Complex Method
The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.
The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:
Tissue staining
Multiple labeling (Multiplex IHC)
Western blotting
Southern and northern blotting
In situ hybridization detection (ISH)
Enzyme immunoassays (ELISA)
Neuronal tracing
All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN ABC system.
Choosing a VECTASTAIN ABC Kit
1) Choose an enzyme system:
The VECTASTAINABC Kits are available with a choice of two different enzyme systems
Peroxidase (ABC, Elite® ABC, RTU or Kits)
Alkaline Phosphatase (ABC-AP)
2) Choose the biotinylated secondary antibody:
To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.
To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN ABC Kit designated “Rabbit IgG”.
For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
3) Choose a convenient format:
Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.
Consider Species Cross-Reactivity
When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:
Biotinylate the primary antibody and detect with a Standard VECTASTAIN Elite ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
Use the M.O.M.® Immunodetection System for applications of mouse primary antibodies on mouse tissue.
Substrates
After choosing the VECTASTAIN ABC Kit for your application, select a substrate that matches the enzyme system of the kit.
Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits (HRP) VECTASTAIN Original ABC Kits (Peroxidase) VECTASTAIN® ABC-HRP Kit, Peroxidase (Rat IgG)
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Description
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Description
The VECTASTAIN Universal Quick HRP Kit can detect any biotinylated molecule. Staining can be completed in less than 20 minutes following primary antibody incubation.
Features:
Rapid protocol: Working solutions can be used immediately after dilution.
High sensitivity, low background
Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*
Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN Universal Kit.
*Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
Sufficient reagents to stain approximately 250-500 sections.
Specifications
More Information
Unit Size
50 ml
Applications
Immunohistochemistry / Immunocytochemistry
Technology
ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s)
Peroxidase
Target Species
Universal (Mouse/Rabbit/Goat)
Format
Ready-to-Use
Kit Components
Kit Contents:
Each of the stabilized, prediluted reagents are supplied in dropper bottles.
50 ml 2.5% normal horse serum
50 ml prediluted, biotinylated, universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
50 ml prediluted peroxidase-streptavidin complex
Documents
Avidin/Biotin Troubleshooting Guide
User Guide
Safety Data Sheet
Download CoA
Datasheet
Product FAQs
How do I choose which VECTASTAIN ABC Kit to use?
We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.
With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
VECTASTAIN Universal Quick Kits
The VECTASTAIN Universal Quick Kit is designed for rapid immunohistochemical staining of tissue sections. The kit relies on a proprietary preformed peroxidase-streptavidin complex to achieve outstanding sensitivity with short incubation times.
Kit Contents:
Each of the stabilized, prediluted reagents are supplied in dropper bottles.
50 ml 2.5% normal horse serum
50 ml prediluted, biotinylated, universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
50 ml prediluted peroxidase-streptavidin complex
The Avidin Biotin Complex Method
The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.
The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:
Tissue staining
Multiple labeling (Multiplex IHC)
Western blotting
Southern and northern blotting
In situ hybridization detection (ISH)
Enzyme immunoassays (ELISA)
Neuronal tracing
All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN ABC system.
Choosing a VECTASTAIN ABC Kit
1) Choose an enzyme system:
The VECTASTAIN ABC Kits are available with a choice of two different enzyme systems
Peroxidase (ABC, Elite ABC, RTU or Kits)
Alkaline Phosphatase (ABC-AP)
2) Choose the biotinylated secondary antibody:
To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.
To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN ABC Kit designated “Rabbit IgG”.
For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
3) Choose a convenient format:
Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.
Consider Species Cross-Reactivity
When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:
Biotinylate the primary antibody and detect with a Standard VECTASTAIN Elite ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
Use the M.O.M.® Immunodetection System for applications of mouse primary antibodies on mouse tissue.
Substrates
After choosing the VECTASTAIN ABC Kit for your application, select a substrate that matches the enzyme system of the kit.
Reconstitute by adding 0.5 ml water. The resulting solution will have the following composition: 10 mM phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide.
The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.
Unconjugated Goat Anti-Human IgG Antibody is supplied in carrier-free, lyophilized form. This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations. It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.
Home All Products Unconjugated Secondary Antibodies Goat Anti-Human IgG Antibody (H+L), Unconjugated
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Description
Unconjugated Goat Anti-Human Kappa Chain Antibody binds to human primary antibodies for immunohistochemistry and many other applications. The unlabeled form can be used as a capture antibody in enzyme immunoassays or radioactively labeled for radioimmunoassays. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.
The goat anti-human Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.
Unconjugated Goat Anti-Human Kappa Chain Antibody is supplied in solution. This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations. It can also be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.
This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies.
Home All Products Unconjugated Secondary Antibodies Goat Anti-Human Antibody, Kappa Chain, Unconjugated
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The goat anti-hamster IgG antibody recognizes IgG from both Syrian and Armenian hamsters. It can be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins.
Features:
Recognizes both heavy and light chains (H+L)
Ready for iodination, fluorochrome labeling, or enzyme conjugations
The goat anti-hamster IgG antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.
This ultrapure, high affinity antibody has been thoroughly adsorbed against serum and immunoglobulins from potentially interfering species and is ready for iodination, fluorochrome labeling, or enzyme conjugations.
Home All Products Unconjugated Secondary Antibodies Goat Anti-Hamster IgG Antibody (H+L), Unconjugated
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The biotinylated goat anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
Recognizes both heavy and light chains (H+L)
Cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is at the same concentration as that provided in the VECTASTAIN® ABC kits.
Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
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The biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide. Immunohistochemical grade bovine serum albumin has been added for stabilization.*
Recommended Usage
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This is not the same antibody as that which is provided in the VECTASTAIN® ABC kits.
Vector Laboratories affinity-purified antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.
With some exceptions the recommended dilution for most applications is 1:200.
Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications. This antibody can be detected with the VECTASTAIN ABC systems or other (strept)avidin reagents.
Home All Products Biotinylated Secondary Antibodies Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated
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This biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Features:
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
Can be used for tissue and cell staining, ELISAs, and blots
Included in the VECTASTAIN® ABC kits
Supplied as solution
Recommended dilution for most applications is 1:50
This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN® Elite ABC Kit (Universal; PK-6200) and VECTASTAIN® ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN®Universal Quick Kit, PK-8800.
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage
The recommended concentration range for use is 10-20 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This product is the same solution as that provided in the VECTASTAIN® Universal ABC Elite® kit (cat. no. PK-6200) and VECTASTAIN® Universal ABC-AP kit (cat. no. AK-5200).
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.
Home All Products Biotinylated Secondary Antibodies Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated
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The biotinylated rabbit anti-sheep IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
Suitable for use with goat, sheep, and bovine IgG primary antibodies
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-sheep IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product is at the same concentration as that provided in the VECTASTAIN® ABC kits.
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody.
With some exceptions the recommended dilution for most applications is 1:200.
The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.
Home All Products Biotinylated Secondary Antibodies Rabbit Anti-Sheep IgG Antibody (H+L), Biotinylated
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