Vector Lab代理Peanut Agglutinin (PNA), CY®3

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Peanut Agglutinin (PNA), CY®3

CL-1073-1

SKU Unit Size Price Qty
CL-1073-1 1 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Peanut Agglutinin (PNA), CY®3

Description
Specifications
Documents
Related Products
Technical Information

Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

Cy3 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation: 552 nm
  • Emission: 565 nm
  • Color: Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 552 nm
Inhibiting and/or Eluting Sugar Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Maximum Emission 565 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
Concentration 1 mg active conjugate/ml
Conjugate Cy3
Color of Fluorescence Red
Sugar Specificity Galactose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Related Products

Peanut Agglutinin (PNA), CY®3

Peanut Agglutinin (PNA), Rhodamine Catalog #: RL-1072-5

Peanut Agglutinin (PNA), CY®3

Galactose Catalog #: S-9003

Peanut Agglutinin (PNA), CY®3

Peanut Agglutinin (PNA), Biotinylated Catalog #: B-1075-5

Technical Information

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM galactose

Home Peanut agglutinin (PNA) Peanut Agglutinin (PNA), CY®3

Vector Lab代理Peanut Agglutinin (PNA), CY®5

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Peanut Agglutinin (PNA), CY®5

CL-1075-1

SKU Unit Size Price Qty
CL-1075-1 1 mg

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Peanut Agglutinin (PNA), CY®5

Description
Specifications
Documents
Related Products
Technical Information

Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

Cy5 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 650 nm
Inhibiting and/or Eluting Sugar Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer.
Concentration 1 mg active conjugate/ml
Conjugate Cy5
Color of Fluorescence Far Red
Sugar Specificity Galactose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Related Products

Peanut Agglutinin (PNA), CY®5

Peanut Agglutinin (PNA), Rhodamine Catalog #: RL-1072-5

Peanut Agglutinin (PNA), CY®5

Galactose Catalog #: S-9003

Peanut Agglutinin (PNA), CY®5

Peanut Agglutinin (PNA), CY®3 Catalog #: CL-1073-1

Peanut Agglutinin (PNA), CY®5

Peanut Agglutinin (PNA), Biotinylated Catalog #: B-1075-5

Technical Information

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM galactose

Home Peanut agglutinin (PNA) Peanut Agglutinin (PNA), CY®5

Vector Lab代理Sambucus Nigra Lectin (SNA, EBL), CY®5

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Sambucus Nigra Lectin (SNA, EBL), CY®5

CL-1305-1

SKU Unit Size Price Qty
CL-1305-1 1 mg

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  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Sambucus Nigra Lectin (SNA, EBL), CY®5

Description
Specifications
Documents
Related Products
Technical Information

Description

Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.

Cy5 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 650 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer
Concentration 1 mg active conjugate/ml
Conjugate Cy5
Color of Fluorescence Far Red
Sugar Specificity Sialic Acid

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Related Products

Sambucus Nigra Lectin (SNA, EBL), CY®5

Sambucus Nigra Lectin (SNA, EBL), Fluorescein Catalog #: FL-1301-2

Sambucus Nigra Lectin (SNA, EBL), CY®5

Sambucus Nigra Lectin (SNA, EBL), Unconjugated Catalog #: L-1300-5

Sambucus Nigra Lectin (SNA, EBL), CY®5

Lactose Catalog #: S-9004

Sambucus Nigra Lectin (SNA, EBL), CY®5

Sambucus Nigra Lectin (SNA, EBL), CY®3 Catalog #: CL-1303-1

Sambucus Nigra Lectin (SNA, EBL), CY®5

Sambucus Nigra Lectin (SNA, EBL), Biotinylated Catalog #: B-1305-2

Sambucus Nigra Lectin (SNA, EBL), CY®5

Sambucus Nigra Lectin (SNA, EBL), Agarose bound Catalog #: AL-1303-2

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 
Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid

Home Sambucus nigra agglutinin (SNA) Sambucus Nigra Lectin (SNA, EBL), CY®5

Vector Lab代理Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Ulex Europaeus Agglutinin I (UEA I), Fluorescein

FL-1061

SKU Unit Size Price Qty
FL-1061-2 2 mg

FL-1061-5 5 mg

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  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
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Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Description
Specifications
Documents
Related Products
Citations
Technical Information

Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Fluorescein labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

More Information
Unit Size 2 mg, 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration FL-1061-2 (2 mg active conjugate/ml)
FL-1061-5 (5 mg active conjugate/ml)
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Fucose, Arabinose

Documents

  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Ulex Europaeus Agglutinin I (UEA I), Rhodamine Catalog #: RL-1062-2

Ulex Europaeus Agglutinin I (UEA I), Fluorescein

L-fucose Catalog #: S-9007

Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Ulex Europaeus Agglutinin I (UEA I), Biotinylated Catalog #: B-1065-2

Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Ulex Europaeus Agglutinin I (UEA I), Agarose bound Catalog #: AL-1063-2

Citations

Ulex Europaeus Agglutinin I (UEA I), Fluorescein Powered by Bioz See more details on Bioz

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose

Home Ulex europaeus I (UEA I) Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Vector Lab代理Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

DL-1208-.5

SKU Unit Size Price Qty
DL-1208-.5 0.5 mg

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  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
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Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Description
Specifications
Documents
Product FAQs
Related Products
Technical Information

Description

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. 

DyLight® 649 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight® 649 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

  • Excitation maximum:  646 nm
  • emission maximum:  674 nm
  • Color:  Red

Specifications

More Information
Unit Size 0.5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 655 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 649
Color of Fluorescence Far Red
Sugar Specificity Galactose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

Can the biotinylated Lycopersicon esculentum (tomato) lectin be used for in vivo perfusion studies to trace blood vessels in mice?

The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

Related Products

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Fluorescein Catalog #: FL-1201-.5

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Galactose Catalog #: S-9003

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Unconjugated Catalog #: L-1104-1

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 594 Catalog #: DL-1207-.5

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, Biotinylated Catalog #: B-1205-.5

Technical Information

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

Home Griffonia simplicifolia I isolectin B4 (GSL I IB4) Griffonia Simplicifolia Lectin I (GSL I) isolectin B4, DyLight® 649

Vector Lab代理ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

MP-7724

SKU Unit Size Price Qty
MP-7724-15 1 kit

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

Description
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Documents
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Technical Information

Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Mouse IgG and ImmPRESS AP Anti-Rabbit IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

 

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

  • Safety Data Sheet
  • User Guide
  • ImmPRESS Polymer & Automated Staining App. Note
  • Download CoA
  • Datasheet

Related Products

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® Vector® Red Substrate, Alkaline Phosphatase (AP) Catalog #: SK-5105

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent Catalog #: MP-6402-15

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) Catalog #: MP-7714

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPRESS® HRP Goat Anti-Rat IgG Polymer Detection Kit, Peroxidase Catalog #: MP-7404-50

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPRESS® HRP Horse Anti-Mouse IgG, Rat adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7422-15

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPRESS® HRP Goat Anti-Rat IgG, Mouse adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7444-15

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

Normal Horse Serum Blocking Solution, 2.5% Catalog #: S-2012-50

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® SG Substrate, Peroxidase (HRP) Catalog #: SK-4705

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® NovaRED® Substrate, Peroxidase (HRP) Catalog #: SK-4805

ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

ImmPACT® AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit. These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

 

Learn more about the ImmPRESS Polymer Detection Systems in double labeling.

Product review on Biocompare.com.

 

 

Home What's New ImmPRESS Duet Double Staining Polymer Kits ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta)

Vector Lab代理Streptavidin, CY®3

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Streptavidin, CY®3

SA-1300-1

SKU Unit Size Price Qty
SA-1300-1 1 mg

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Streptavidin, CY®3

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 550 nm
Maximum Emission 570 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy3
Color of Fluorescence Red
Format Concentrate

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

Streptavidin, CY®3

Anti-Streptavidin Antibody, Biotinylated Catalog #: BA-0500-.5

Streptavidin, CY®3

Streptavidin/Biotin Blocking Kit Catalog #: SP-2002

Streptavidin, CY®3

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

Streptavidin, CY®3

VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10

Streptavidin, CY®3

VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10

Streptavidin, CY®3

VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10

Streptavidin, CY®3

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Streptavidin, CY®3

VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10

Citations

Streptavidin, CY®3 Powered by Bioz See more details on Bioz

Home What's New Fluorophore Conjugated Avidin/Streptavidin Streptavidin, CY®3

Vector Lab代理Animal-Free Blocker® and Diluent, R.T.U.

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

Animal-Free Blocker® and Diluent, R.T.U.

SP-5035-100

SKU Unit Size Price Qty
SP-5035-100 100 ml

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Animal-Free Blocker® and Diluent, R.T.U.

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Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

Specifications

More Information
Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications.This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

Documents

  • Safety Data Sheet
  • User Guide
  • Download CoA
  • Datasheet

Product FAQs

What is the difference between the R.T.U. Animal Free Block and Diluent (SP-5035) and the 5x Animal Free Blocker® (SP-5030)?

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Related Products

Animal-Free Blocker® and Diluent, R.T.U.

Carbo-Free Blocking Solution (10x Concentrate) Catalog #: SP-5040-125

Animal-Free Blocker® and Diluent, R.T.U.

Bovine Serum Albumin (BSA), Immunohistochemical Grade Catalog #: SP-5050-500

Animal-Free Blocker® and Diluent, R.T.U.

10x Casein Solution Catalog #: SP-5020-250

Animal-Free Blocker® and Diluent, R.T.U.

Animal-Free Blocker®, 5X Concentrate Catalog #: SP-5030-250

Technical Information

This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. 

Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 

Home What's New Protein Blocking Solutions Animal-Free Blocker® and Diluent, R.T.U.

Vector Lab代理ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

MP-7405

SKU Unit Size Price Qty
MP-7405-15 15 ml

MP-7405-50 50 ml

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ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

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Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced staining of nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Goat IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

More Information
Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Goat
Format Ready-to-Use

Documents

  • ImmPRESS Polymer & Automated Staining App. Note
  • ImmPRESS Troubleshooting Guide
  • User Guide
  • Safety Data Sheet
  • Download CoA
  • Datasheet

Product FAQs

What are the advantages of using ImmPRESS® Excel kits compared with one-step peroxidase polymer detection systems?

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Our research lab has an open automated staining platform. Do you know if the ImmPRESS reagents can be applied to an automated system, and if so, do you have any guidelines or procedures?

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://info.vectorlabs.com/l/360741/2019-01-11/2hv9mw

Related Products

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® DAB Substrate, Peroxidase (HRP) Catalog #: SK-4105

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® NovaRED® Substrate, Peroxidase (HRP) Catalog #: SK-4805

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® VIP Substrate, Peroxidase (HRP) Catalog #: SK-4605

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® SG Substrate, Peroxidase (HRP) Catalog #: SK-4705

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® AEC Substrate, Peroxidase (HRP) Catalog #: SK-4205

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

ImmPACT® AMEC Red Substrate, Peroxidase (HRP) Catalog #: SK-4285

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Vector® NovaRED® Substrate Kit, Peroxidase (HRP) Catalog #: SK-4800

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine) Catalog #: SK-4100

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

TMB Substrate Kit, Peroxidase (HRP), (3,3', 5,5'-tetramethylbenzidine) Catalog #: SK-4400

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Vector® SG Substrate Kit, Peroxidase (HRP) Catalog #: SK-4700

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Vector® VIP Substrate Kit, Peroxidase (HRP) Catalog #: SK-4600

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

AEC Substrate Kit, Peroxidase (HRP), (3-amino-9-ethylcarbazole) Catalog #: SK-4200

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Normal Horse Serum Blocking Solution, 2.5% Catalog #: S-2012-50

Citations

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase Powered by Bioz See more details on Bioz

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

 

Learn more about the ImmPRESS Polymer Detection Systems in double labeling.

Product review on Biocompare.com.

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Vector Lab代理VectaCell Trolox Antifade Reagent for Live Cell Imaging

Vector Labs代理,欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司,七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命,该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展,以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商,并提供大量的新产品,如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。

VectaCell Trolox Antifade Reagent for Live Cell Imaging

CB-1000-2

SKU Unit Size Price Qty
CB-1000-2 2 ml

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VectaCell Trolox Antifade Reagent for Live Cell Imaging

Description
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Technical Information

Description

VectaCell Trolox is an antifading additive for live cell imaging. It is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of various reactive oxygen species, such as singlet oxygen (1O2), superoxide anion (O2) or hydrogen peroxide (H2O2).

Specifications

More Information
Unit Size 2 ml
Applications Immunofluorescence, Cellular Imaging
Concentration 100 mM (in ethanol)
Recommended Usage Instructions for use: Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. Notes: The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay.
Recommended Storage 2-8 °C
Microscopy Fluorescence
Antifade Yes

Documents

  • Safety Data Sheet
  • Download CoA
  • Datasheet

Related Products

VectaCell Trolox Antifade Reagent for Live Cell Imaging

VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10

VectaCell Trolox Antifade Reagent for Live Cell Imaging

VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10

Technical Information

VectaCell Trolox contains both Trolox and its oxidized form Trolox-quinone. This system reduces photo-bleaching and blinking during live cell imaging,1, 2 and has also been shown to have a cytoprotective effect and low cytotoxicity for different cell lines.3

Trolox has been used to reduce fluorophore bleaching1 as well as the fluorophore blinking2 during the observation of fluorescent labels. Although the mechanism is not completely understood, it has been attributed to the combination of its oxygen-scavenger capacity that prevents oxidative bleaching and the complementary reduction reaction that helps prevent blinking.

VectaCell Trolox is supplied as a convenient 100 mM stock solution.

References:

  1. Rasnik et al. Nonblinking and long-lasting single-molecule fourorescence imaging. (2006) Nat. Meth. 3:891-893
  2. Cordes et al. On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent. (2009) J. Am. Chem. Soc. 131:5018-5019
  3. Wu et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. (1990) Biochem. Cell Biol. 68: 1189-1194

Home Live Cell Imaging Solutions VectaCell Trolox Antifade Reagent for Live Cell Imaging