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Peanut Agglutinin (PNA), CY®3
Description
Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.
Cy3 labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
- Excitation: 552 nm
- Emission: 565 nm
- Color: Red
Specifications
Unit Size | 1 mg |
---|---|
Applications | Immunofluorescence, Glycobiology |
Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
Recommended Storage | 2-8 °C |
Maximum Excitation | 552 nm |
Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: 200 mM galactose (S-9003). |
Maximum Emission | 565 nm |
Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer. |
Concentration | 1 mg active conjugate/ml |
Conjugate | Cy3 |
Color of Fluorescence | Red |
Sugar Specificity | Galactose |
Documents
- Safety Data Sheet
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Download CoA
- Datasheet
Related Products

Peanut Agglutinin (PNA), Rhodamine Catalog #: RL-1072-5

Galactose Catalog #: S-9003

Peanut Agglutinin (PNA), Biotinylated Catalog #: B-1075-5
Technical Information
A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.
Inhibiting/Eluting Sugar: 200 mM galactose