GE 17-0851-01 Disposable PD-10脱盐层析柱

GE 17-0851-01 Disposable PD-10脱盐层析柱
产品名称: GE 17-0851-01
英文名称:Disposable PD-10脱盐层析柱
产品型号: 17-0851-01
代理商:上海金畔生物
简单介绍
GE 17-0851-01 Disposable PD-10脱盐层析柱PD-10脱盐层析柱与一个方便的直立层析柱架一起提供。使用LabMate缓冲液储液器使柱平衡过程更容易进行。 • 快速、高回收率的脱盐和缓冲液置换,也可用于除小分子化合物;预装Sephadex G-25填料。 • 操作灵活,可用离心或重力流。 • 容易使用,便于平衡、上样、洗脱。

Sephadex G-25 in PD-10 Desalting Columns

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

  • Flexible run protocols – perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
  • Flexible throughput – desalt one sample or multiple samples in parallel
  • Good recovery – in the range of 70% to > 95%
  • High desalting capacity – remove > 98% salt with gravity; > 90% with centrifugation
  • Minimal dilution – typically none when used as spin columns; 1.4× when used with gravity flow
  • Time savings compared to dialysis – inexpensive and convenient alternative to lengthy and troublesome dialysis procedures

What comes in the PD-10 column package?

Each product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.

Versatile for use with centrifugation or gravity flow

The product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Successful use of these prepacked desalting columns

The following publications demonstrate the utility of PD-10 columns:

  • desalting of membrane proteins after anion exchange chromatography
  • desalting of variants of an essential reductase involved in fatty acid biosynthesis
  • buffer exchange of an affinity-tagged acyl carrier protein

Accessories for PD-10 Desalting Columns

A LabMate buffer reservoir can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.

Other Sephadex G-25 columns from GE

GE Healthcare’s Life Sciences solutions for desalting include a wide range of columns containing Sephadex G-25 resin. Options are available to accommodate sample volumes from 100 µL to 15 mL and use with manual methods or a chromatography system. Let GE help you find the right desalting product.

GE 17-0851-01 Disposable PD-10脱盐层析柱的详细介绍

GE 17-0851-01 Disposable PD-10脱盐层析柱PD-10脱盐层析柱与一个方便的直立层析柱架一起提供。使用LabMate缓冲液储液器使柱平衡过程更容易进行。

•  快速、高回收率的脱盐和缓冲液置换,也可用于除小分子化合物;预装Sephadex G-25填料。

•  操作灵活,可用离心或重力流。

•  容易使用,便于平衡、上样、洗脱。

•  重力流方式样品量1-2.5ml;离心方式样品量1.75-2.5ml。平行处理多个待脱盐样品,可除去90%以上盐离子。

•  蛋白回收率在70%-95%。

•  LabMate Buffer Reservoir使得平衡PD-10层析柱更容易,更方便。

PD-10层析柱提供了一个经济、方便的选择,可取代冗长、繁琐的透析过程。几分钟内,完成生物样品脱盐、小分子可溶物的去除或替换,同时有极好的回收率。

Disposable PD-10 Desalting Columns经过改进设计,能应用于两种新操作方式:重力流和离心,更为灵活方便。重力流操作可选用一到数根柱子,平行操作多个样品进行除杂处理,而无需纯化设备。离心操作则能以标准的离心程序平行处理洗脱样品,将样品稀释降低到最少。

每个包装内含有四个接头,方便连接配置。与LabMate Buffer Reservoir(需另行购买)联合使用进行重力流操作时,清洗和平衡用的缓冲液能在同一步中加入。
如需要设计经济适用,通用性好的制备样品工艺,推荐购买PD-10 columns和批量包装的填料。

Disposable PD-10脱盐预装层析柱每盒有30根。每根柱均保存在含有0.15% Kathon CG保护剂的蒸馏水中。颗粒大小为20-85μm的填料填充在两个烧结的聚乙烯熔块中。熔块保护填料免于在重力缓冲液流下而干柱。层析柱的出口用可重复使用的帽封口。

重力流操作,从BSA中除去NaCl。样品回收率95%,除盐99%以上。

PD-10 Desalting Columns能以重力流或离心方式快速脱盐。蓝色接头用于离心操作

GE多模式层析预装柱

GE多模式层析预装柱

货号 名称
28926980 HiScreen Capto MMC
17548115 HiScreen Capto Core 700
17371620 HiScreen Capto MMC ImpRes
17371520 HiScreen Capto adhere ImpRes
28926981 HiScreen Capto Adhere
11003273 HITRAP CAPTO MMC, 5X1ML
17548151 HiTrap Capto Core 700, 5 x 1 ml
28405844 HiTrap Capto Adhere 5x1ml
17371610 HiTrap Capto MMC ImpRes 5×1 ml
17371510 HiTrap Capto adhere ImpRes 5×1 ml
11003275 HITRAP CAPTO MMC, 5X5ML
28405846 5×5 ml Hitrap Capto adhere
28908406 Capto MMC, XK 26/20

GE凝胶过滤预装柱

GE凝胶过滤预装柱

货号 名称
29048684 HITRAP DESALTING, 1X5ML
18117643 STORAGE/SHIPPING DEVICE
17140801 HITRAP DESALTING, 5X5ML
17036001 BLUE DEXTRAN 2000 10 G
28403841 GEL FILTRATION CAL KIT LMW
28403842 GEL FILTRATION CAL KIT HMW
17508701 HIPREP 26/10 DESALTING
17116501 HIPREP 16/60 SEPHACRYL S-100 HR
17116601 HIPREP 16/60 SEPHACRYL S-200 HR
17116701 HIPREP 16/60 SEPHACRYL S-300 HR
28935604 HiPrep 16/60 Sephacryl S-400 HR
28935606 HiPrep 16/60 Sephacryl S-500 HR
17145501 PRECISION COLUMN HOLDER
17119401 HIPREP 26/60 SEPHACRYL S-100 HR
17119501 HIPREP 26/60 SEPHACRYL S-200 HR
17119601 HIPREP 26/60 SEPHACRYL S-300 HR
28935605 HiPrep 26/60 Sephacryl S-400 HR
28935607 HiPrep 26/60 Sephacryl S-500 HR
28990944 Superdex 200 Increase 10/300 GL
28990945 Superdex 200 Increase 5/150 GL
29091596 Superose 6 Increase 10/300 GL
29091597 Superose 6 Increase 5/150 GL
29148721 Superdex 75 Increase 10/300 GL
29148722 Superdex 75 Increase 5/150 GL
29219757 Superdex 30 Increase 10/300 GL
17508702 HIPREP 26/10 DESALTING, 4 PACK
28990946 Superdex 200 Increase 3.2/300
29091598 Superose 6 Increase 3.2/300
29148723 Superdex 75 Increase 3.2/300
29219758 Superdex 30 Increase 3.2/300
28989331 HiLoad 16/600 Superdex 30 pg
28989333 HiLoad 16/600 Superdex 75 pg
28989335 HiLoad 16/600 Superdex 200 pg
28989332 HiLoad 26/600 Superdex 30 pg
28989334 HiLoad 26/600 Superdex 75 pg
28989336 HiLoad 26/600 Superdex 200 pg

GE其他亲和层析预装柱

GE其他亲和层析预装柱

货号 名称
17071701 HITRAP NHS-ACTIV HP, 1 X 5 ML
17041301 HITRAP BLUE HP, 1 X 5 ML
17071601 HITRAP NHS-ACTIV HP, 5 X 1ML
17514302 HITRAP BENZAMIDINE FF 2 X 1 ML
17040701 HITRAP HEPARIN HP,1 X 5 ML
17041201 HITRAP BLUE HP, 5 X 1 ML
28952085 HiTrap Con A 4B 5 x 1 ml
28978243 HiScreen Blue FF
28992474 HiScreen Capto Blue
17040601 HITRAP HEPARIN HP, 5 X 1 ML
17514401 HITRAP BENZAMIDINE FF 1 X 5 ML
17514301 HITRAP BENZAMIDINE FF 5 X 1ML
17511201 HITRAP STREPTAVIDIN HP5X1 ML
28948020 Albumin IgG Depletion SpinTrap
28952096 HiTrap Con A 4B 5 x 5 ml
28936549 HiPrep Heparin FF 16/10
17040703 HiTrap Heparin HP 5x5ml
17548911 HiTrap 5x1ml Capto Lentil Lectin
28946603 HiTrap Albumin IgG Depl,2×1 ml
28411212 HiTrap 1x5ml, AVB Sepharose HP
28411211 HiTrap 5×1 ml, AVB Sepharose HP
28908366 HiTrap 5x5ml, IgG Sepharose FF
28903131 Streptavidin HP MultiTrap
17548912 HiTrap 5×5 ml Capto Lentil Lectin

GE亲和层析抗体纯化预装柱

GE亲和层析抗体纯化预装柱

货号 名称
29048576 HITRAP PROTEIN A HP, 1 X 1 ML
29048581 HITRAP PROTEIN G HP, 1 X 1 ML
29048665 HiTrap Protein L, 1x1mL
29049104 HITRAP MABSELECT SURE, 1X1ML
17040203 HITRAP PROTEIN A HP,2 X 1 ML
17040403 HITRAP PROTEIN G HP, 2 X 1 ML
17507902 HITRAP RPROTEIN A FF, 2 X 1 ML
17112801 MABTRAP KIT
17040301 HITRAP PROTEIN A HP,1 X 5 ML
17040501 HITRAP PROTEIN G HP,1 X 5 ML
17040201 HITRAP PROTEIN A HP, 5 X 1 ML
17508001 HITRAP RPROTEIN A FF, 1X5ML
17511001 HITRAP IGM PURIFICATION 5X1ML
17511101 HITRAP IGY PURIFICATION 1X5 ML
28408347 Ab SpinTrap
17040401 HITRAP PROTEIN G HP, 5 X 1 ML
17547815 HiTrap Protein L, 1x5mL
28408255 HITRAP MABSELECT, 1 X 5 ML
28408260 HITRAP MABSELECT XTRA, 1X5ML
17547851 HiTrap Protein L, 5x1mL
17507901 HITRAP RPROTEIN A FF, 5X1ML
28408253 HITRAP MABSELECT, 5 X 1 ML
28408258 HITRAP MABSELECT XTRA, 5X1ML
17545812 HiTrap 1×5 ml, KappaSelect
17548212 HiTrap 1×5 ml, LambdaFabSelect
17549112 HiTrap 1x5ml MabSelect SuRe pcc
17545811 HiTrap 5×1 ml, KappaSelect
17548211 HiTrap 5×1 ml, LambdaFabSelect
28985254 rProtein A GraviTrap
28985255 Protein G GraviTrap
28985256 rProtein A/Protein G GraviTrap
11003494 HITRAP MABSELECT SURE, 1X5ML
17547814 HiScreen Capto L
28926973 HiScreen MabSelect
17549111 HiTrap 5×1 ml MabSelect SuRe pcc
28926976 HiScreen MabSelectXtra
11003493 HITRAP MABSELECT SURE, 5X1ML
28926977 HiScreen MabSelectSuRe
28411312 HiTrap 1x5ml, IgSelect
28411311 HiTrap 5x1ml, IgSelect
17040303 HITRAP PROTEIN A HP, 5 X 5 ML
17040503 HITRAP PROTEIN G HP, 5 X 5 ML
17547855 HiTrap Protein L, 5x5mL
17508002 HITRAP RPROTEIN A FF, 5 X 5 ML
28408256 HITRAP MABSELECT, 5 X 5 ML
28408261 HITRAP MABSELECT XTRA, 5X5ML
11003495 HITRAP MABSELECT SURE, 5X5ML

GE亲和层析标签蛋白纯化预装柱

GE亲和层析标签蛋白纯化预装柱

货号 名称
27152401 M13K07 WI 1 V
27084601 THROMBIN
11003400 HIS BUFFER KIT
27141001 5 PGEX SEQUENCING PRIMER
27141101 3 PGEX SEQUENCING PRIMER
11003399 HIS GRAVITRAP
29000594 His GraviTrap TALON
27084301 PRESCISSION PROTEASE
29000593 His SpinTrap TALON
27457701 ANTI-GST ANTIBODY
28952360 GST GraviTrap
28954648 PGEX-6P-1 VECTOR
28954651 PGEX-6P-3 VECTOR
28954653 PGEX-2T 25 UG WI/DI
28954549 PGEX-4T-1
28954552 PGEX-4T-3
27457001 GST Bulk Kit
29000596 His MultiTrap TALON
28918779 MBPTrap 1 x 5 mL
17513101 GSTRAP FF, 1X5ML
28918778 MBPTrap 5 x 1mL
17513001 GSTRAP FF, 5X1ML
28401747 GSTRAP 4B, 1 X 5 ML
28401745 GSTRAP 4B, 5 X 1 ML
17528201 GSTRAP HP 1 X 5 ML
17528101 GSTRAP HP 5 X 1 ML
28936552 HiPrep IMAC FF 16/10
28907547 StrepTrap HP 1 x 5mL
28936551 HisPrep FF 16/10
17092104 HITRAP IMAC FF, 5 X 5 ML
17525501 HISTRAP FF 5 X 5 ML
17524802 HISTRAP HP 5 X 5 ML
17092005 HITRAP IMAC HP, 5 X 5 ML
17040903 HITRAP CHELATING HP 5 X 5 ML
17528601 HISTRAP FF CRUDE 5X5 ML
28953767 HiTrap TALON crude, 5 x 5 ml
28936550 GSTPrep FF 16/10
17371206 HisTrap excel 5×5 ml
28907546 StrepTrap HP 5 x 1mL
90100480 HITRAP 5X5ML CHELATING SEPH FF
28918780 MBPTrap 5 x 5mL
17513102 GSTRAP FF 5 X 5 ML
28401748 GSTRAP 4B, 5 X 5 ML
17528202 GSTRAP HP 5 X 5 ML
28907548 StrepTrap HP 5 x 5mL

GE蛋白提取试剂和纯化配套缓冲液

GE蛋白提取试剂和纯化配套缓冲液

货号 名称
28941279 Mammalian Cell Lysis Buffer
80650142 NUCLEASE MIX
28944045 Yeast Buffer Kit
28943522 Extraction Buffer Trial Kit
80650123 PROTEASE INHIBITOR MIX
28913568 Streptavidin HP SpinTrap Buffer kit
80648451 2-D CLEAN-UP KIT
80648470 SDS-PAGE CLEAN-UP KIT
80648356 2-D QUANT KIT
80648337 SAMPLE GRINDING KIT

GE脱盐柱、超滤管和空柱

GE脱盐柱、超滤管和空柱

货号 名称
28923243 MiniSpin Adapter
28923244 MidiSpin Adapter
28923245 PD-10 Spin Adapter
18321603 LABMATE PD-10 BUFFER RESERVOIR
28403943 COLLECTION PLATE
28932218 Vivaspin 500, 3 kDa MWCO PES
28932223 Vivaspin 500, 5 kDa MWCO PES
28932225 Vivaspin 500, 10 kDa MWCO PES
28932235 Vivaspin 500, 30 kDa MWCO PES
28932236 Vivaspin 500, 50 kDa MWCO PES
28932237 Vivaspin 500, 100 kDa MWCO PES
28932240 Vivaspin 2, 3 kDa MWCO PES
28932245 Vivaspin 2, 5 kDa MWCO PES
28932247 Vivaspin 2, 10 kDa MWCO PES
28932248 Vivaspin 2, 30 kDa MWCO PES
28932257 Vivaspin 2, 50 kDa MWCO PES
28932258 Vivaspin 2, 100 kDa MWCO PES
28932358 Vivaspin 20, 3 kDa MWCO PES
28932359 Vivaspin 20, 5 kDa MWCO PES
28932360 Vivaspin 20, 10 kDa MWCO PES
28932361 Vivaspin 20, 30 kDa MWCO PES
28932362 Vivaspin 20, 50 kDa MWCO PES
28932363 Vivaspin 20, 100 kDa MWCO PES
28932293 Vivaspin 6, 3 kDa MWCO PES
28932294 Vivaspin 6, 5 kDa MWCO PES
28932296 Vivaspin 6, 10 kDa MWCO PES
28932317 Vivaspin 6, 30 kDa MWCO PES
28932318 Vivaspin 6, 50 kDa MWCO PES
28932319 Vivaspin 6, 100 kDa MWCO PES
17043501 COLUMN PD-10,EMPTY
17085101 COLUMN PD 10
28918004 PD SpinTrap G-25
80648375 MINI DIALYSIS KIT,1 KDA,250 UL
80648413 MINI DIALYSIS KIT,8 KDA,250 UL
80648432 MINI DIALYSIS KIT,8 KDA,2 ML
80648394 MINI DIALYSIS KIT,1 KDA,2 ML
28918008 PD MidiTrap G-25
28918010 PD MiniTrap G-10
28918007 PD MiniTrap G-25
28918011 PD MidiTrap G-10
28918006 PD MultiTrap G-25

GE多模式填料

GE多模式填料

货号 名称
17531710 CAPTO MMC, 25ML
17371601 Capto MMC ImpRes, 25 ml
17548101 Capto Core 700, 25 mL
17371501 Capto adhere ImpRes, 25 ml
17544410 CAPTO adhere 25 ML
17531702 Capto MMC 100ml
17548102 Capto Core 700, 100 mL
17371602 Capto MMC ImpRes, 100 ml
17544401 Capto adhere 100 ml
17371502 Capto adhere ImpRes, 100 ml