标签归档：GE

GE蛋白质手动纯化试剂

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GE蛋白质手动纯化试剂

货号 名称
27152401 M13K07 WI 1 V
27084601 THROMBIN
11003400 HIS BUFFER KIT
27141001 5 PGEX SEQUENCING PRIMER
27141101 3 PGEX SEQUENCING PRIMER
11003399 HIS GRAVITRAP
29000594 His GraviTrap TALON
27084301 PRESCISSION PROTEASE
29000593 His SpinTrap TALON
27457701 ANTI-GST ANTIBODY
28952360 GST GraviTrap
28954648 PGEX-6P-1 VECTOR
28954651 PGEX-6P-3 VECTOR
28954653 PGEX-2T 25 UG WI/DI
28954549 PGEX-4T-1
28954552 PGEX-4T-3
27457001 GST Bulk Kit
29000596 His MultiTrap TALON

GE蛋白提取试剂和纯化配套缓冲液

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GE蛋白提取试剂和纯化配套缓冲液

货号 名称
28941279 Mammalian Cell Lysis Buffer
80650142 NUCLEASE MIX
28944045 Yeast Buffer Kit
28943522 Extraction Buffer Trial Kit
80650123 PROTEASE INHIBITOR MIX
28913568 Streptavidin HP SpinTrap Buffer kit
80648451 2-D CLEAN-UP KIT
80648470 SDS-PAGE CLEAN-UP KIT
80648356 2-D QUANT KIT
80648337 SAMPLE GRINDING KIT

GE脱盐柱、超滤管和空柱

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GE脱盐柱、超滤管和空柱

货号 名称
28923243 MiniSpin Adapter
28923244 MidiSpin Adapter
28923245 PD-10 Spin Adapter
18321603 LABMATE PD-10 BUFFER RESERVOIR
28403943 COLLECTION PLATE
28932218 Vivaspin 500, 3 kDa MWCO PES
28932223 Vivaspin 500, 5 kDa MWCO PES
28932225 Vivaspin 500, 10 kDa MWCO PES
28932235 Vivaspin 500, 30 kDa MWCO PES
28932236 Vivaspin 500, 50 kDa MWCO PES
28932237 Vivaspin 500, 100 kDa MWCO PES
28932240 Vivaspin 2, 3 kDa MWCO PES
28932245 Vivaspin 2, 5 kDa MWCO PES
28932247 Vivaspin 2, 10 kDa MWCO PES
28932248 Vivaspin 2, 30 kDa MWCO PES
28932257 Vivaspin 2, 50 kDa MWCO PES
28932258 Vivaspin 2, 100 kDa MWCO PES
28932358 Vivaspin 20, 3 kDa MWCO PES
28932359 Vivaspin 20, 5 kDa MWCO PES
28932360 Vivaspin 20, 10 kDa MWCO PES
28932361 Vivaspin 20, 30 kDa MWCO PES
28932362 Vivaspin 20, 50 kDa MWCO PES
28932363 Vivaspin 20, 100 kDa MWCO PES
28932293 Vivaspin 6, 3 kDa MWCO PES
28932294 Vivaspin 6, 5 kDa MWCO PES
28932296 Vivaspin 6, 10 kDa MWCO PES
28932317 Vivaspin 6, 30 kDa MWCO PES
28932318 Vivaspin 6, 50 kDa MWCO PES
28932319 Vivaspin 6, 100 kDa MWCO PES
17043501 COLUMN PD-10,EMPTY
17085101 COLUMN PD 10
28918004 PD SpinTrap G-25
80648375 MINI DIALYSIS KIT,1 KDA,250 UL
80648413 MINI DIALYSIS KIT,8 KDA,250 UL
80648432 MINI DIALYSIS KIT,8 KDA,2 ML
80648394 MINI DIALYSIS KIT,1 KDA,2 ML
28918008 PD MidiTrap G-25
28918010 PD MiniTrap G-10
28918007 PD MiniTrap G-25
28918011 PD MidiTrap G-10
28918006 PD MultiTrap G-25

GE多模式填料

发表于由

GE多模式填料

货号 名称
17531710 CAPTO MMC, 25ML
17371601 Capto MMC ImpRes, 25 ml
17548101 Capto Core 700, 25 mL
17371501 Capto adhere ImpRes, 25 ml
17544410 CAPTO adhere 25 ML
17531702 Capto MMC 100ml
17548102 Capto Core 700, 100 mL
17371602 Capto MMC ImpRes, 100 ml
17544401 Capto adhere 100 ml
17371502 Capto adhere ImpRes, 100 ml

GE其他亲和层析填料

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GE其他亲和层析填料

货号 名称
17044003 CON-A SEPHAROSE 4B 5 ML
17544801 Capto Blue 25 ml
17095601 GELATIN-SEPHAROSE 4B, 25 ML
17090601 NHS ACT SEPHAROSE 4 FF 25 ML
17098101 CNBr Activated Sepharose 4FF,10G
17094801 BLUE SEPHAROSE 6 FAST FLOW, 50ML
17512310 BENZAMIDIN SEPH 4FF H-SUB 25ML
17043001 CNBR ACTIVATED SEPHAROSE 4B 15 G
17096901 IGG SEPHAROSE 6 FAST FLOW 10 ML
17056901 EAH-SEPHAROSE 4B, 50 ML
17052901 CALMODULIN-SEPHAROSE 4B, 10 ML
17511301 STREPTAVIDIN SEPHAROSE HP,5 ML
17099801 HEPARIN SEPHAROSE 6 FF,50 ML
17048001 EPOXY ACTIVATED SEPHAROSE 6B 15 G
17064001 ACT THIOL SEPHAROSE 4B 15 G
17042001 THIOPROPYL SEPHAROSE 6 B 15 G
17044401 LENTIL LECTIN-SEPHAROSE 4 B 25 ML
17044001 CON-A SEPHAROSE 4B 100 ML
17070001 2, 5 ADP-SEPHAROSE 4B 5 G
90100123 BLUE SEPHAROSE HP, 50 ML
90100019 HEPARIN SEPHAROSE HP 50 ML
90100400 NHS-ACT SEPHAROSE HP, 100 ML

GE疏水填料

发表于由

GE疏水填料

GE 疏水层析填料
生物分子表面大都含或强或弱的疏水区域，在不同环境下，与各种疏水介质产生不同强弱和结合。
高离子强度可加强疏水性。和离子交换刚刚相反，高盐吸附，低盐洗脱。经洗脱样品又可直接或稍加稀释后上
其它层析柱，做为连接下游和层析步骤的桥梁。并可完全取代传统的盐析沉淀技术，更符合工业生产要求。
比反相层析介质的配体密度低许多，无需有机溶液剂洗脱，保存生物活性。配体种类繁多，提供宽广的选择性。
疏水层析的分离效果受不同配体、缓冲液、盐浓度、温度等多种因素影响。哪种介质最适合很难预测，必须经过试验。疏水层析试盒[货号17-1349-01 ] 提供了五种不同疏水层析小预装柱，可快速筛选适合介质。
疏水填料选择指导指南
生物分子表面大都含或强或弱的疏水区域，在不同环境下，与各种疏水填料产生不同强弱和结合。
高离子强度可加强疏水性。和离子交换刚刚相反，高盐吸附，低盐洗脱。经洗脱样品又可直接或稍加稀释后上其它层析柱，做为连接下游和层析步骤的桥梁。并可完全取代传统的盐析沉淀技术，更符合工业生产要求。
比反相层析填料的配体密度低许多，无需有机溶液剂洗脱，保存生物活性。配体种类繁多，提供宽广的选择性。
疏水填料疏水性强弱表 （由大到小）
Butyl-S Sepharose 6 Fast Flow
Capto Octyl
Octyl Sepharose 4 Fast Flow
Phenyl Sepharose 6 Fast Flow (low sub)
Capto Butyl
Butyl Sepharose 4 Fast Flow
Phenyl Sepharose High Performance
Capto Phenyl ImpRes
Butyl Sepharose High Performance
Capto Butyl ImpRes
Phenyl Sepharose 6 Fast Flow (high sub)
Capto Phenyl (high sub)
货号 名称
17096510 PHENYL SEPHAROSE 6 FF / LS, 25 ML
17097310 PHENYL SEPHAROSE 6 FF /HS, 25 ML
17098010 BUTYL SEPHAROSE FAST FLOW, 25 ML
17545901 Capto Butyl 25 ml
17545101 Capto Phenyl High Sub 25 ml
17108201 PHENYL SEPHAROSE HIGH PERF. 75 ML
17081001 PHENYL-SEPHAROSE CL-4B 200 ML
17079001 OCTYL-SEPHAROSE CL-4B 200 ML
17094602 OCTYL SEPHAROSE 4 FAST FLOW 200 ML
17097305 PHENYL SEPHAROSE 6FF (HS) 200 ML
17096505 PHENYL SEPHAROSE 6 FF LS 200 ML
17098001 BUTYL SEPHAROSE 4 FAST FLOW 200 ML
17097802 BUTYL-S SEPHAROSE 6 FF, 200 ML
17543202 Butyl Sepharose HP, 200ml

一、预处理及中度纯化介质

货号  产品  包装  每毫升载量  颗粒 大小(μm)  每毫升 结合量  特性／应用  pH 稳定性 工作[清洗]  zui快流速(cm/h)
17-0980-10 17-0980-01 17-0980-02 17-0980-04 Butyl Sepharose 4 Fast Flow 25 ml 200 ml 500 ml 5 L 50 μ mole 正丁烷基n-Butyl 45-165 7 mg lgG 26 mg HSA 疏水性zui弱，适合含脂族(aliphatic ) 配体的生物分子 3-13 [2-14] 600
17-5226-04 Butyl Sepharose 4FF high sub 5 L 正丁烷基 45-165 同上 3-13 [2-14] 600
17-0946-10 17-0946-02 17-0946-03 17-0946-04 Octyl Sepharose 4 Fast Flow 25 ml 200 ml 1 L 5 L 50 μ mole 正辛烷基n-Octyl 45-165 26 mg lgG 7 mg HSA 疏水性中等，适合各种蛋白的分离和纯化 3-13 [2-14] 600
17-0965-10 17-0965-05 17-0965-03 17-0965-04 Phenyl Sepharose 6 Fast Flow (low sub) 25 ml 200 ml 1 L 5 L 20 μ mole 苯基Phenyl 45-165 10 mg lgG 24 mg HSA 适合含芳香族(aromatic ) 配体的蛋白，特别是单抗 3-13 [2-14] 600
17-0973-10 17-0973-05 17-0973-03 17-0973-04 Phenyl Sepharose 6 Fast Flow (high sub) 25 ml 200 ml 1 L 5 L 40 μ mole 苯基Phenyl 45-165 30 mg lgG 36 mg HSA 疏水性zui强，载量高，适合含芳香族(aromatic ) 配体的生物分子的预处理 3-13 [2-14] 600
17-5098-03 Phenyl Sepharose Big Beads 1 L 100-300 同上 3-14 [2-14] 1800
17-0978-10 17-0978-02 17-0978-03 Butyl-S Sepharose 6 Fast Flow 25 ml 200 ml 1 L 8.9-11.3 μ moles 90 疏水性zui弱的疏水填料 3-13 [2-14] 600
17-0978-04 5 L

二、中度分离及zui终纯化介质

货号  产品  包装  每毫升载量  颗粒 大小(μm)  每毫升 结合量  特性／应用  pH 稳定性 工作[清洗]  zui快流速(cm/h)
17-1082-01 17-1082-03 17-1082-04 Phenyl Sepharose High Performance 75 ml 1 L 5 L 25 μ mole 苯基Phenyl 24-44 20 mg lgG 24 mg HSA 以高分辨率纯化难以分离的重组蛋白天然形式和修饰变种，及各种单抗 3-12 [2-14] 150
17-5432-03 Butyl Sepharose HP 1 L 正丁烷基 24-44 高分辨率 3-12; [2-14]
三、经典疏水层析介质
17-0960-01 17-0960-03 Butyl Sepharose 4B 50 ml 500 ml 6-14 μ mole 正丁烷基 45-165 视不同样品而定 大分子，复合分离原理包括疏水性及离子性分离 50
17-0960-04 5 L
17-0790-01 17-0790-05 Octyl Sepharose CL-4B 200 ml 10 L 40 μ mole 正辛烷基n-Octyl 45-165 15-20 mg HSA 纯化疏水性较弱的蛋白或膜蛋白，因其经去污剂处理后仍有强疏水性 3-12 [2-14] 50
17-0810-01 17-0810-05 Phenyl Sepharose CL-4B 200 ml 10 L 40 μ mole 苯基Phenyl 45-165 15-20 mg HSA 疏水性较Octyl 弱，适用于分离和纯化对疏水性尚未了解的蛋白 3-12 [2-14] 50

STREAMLINE

货号  产品  包装  颗粒 大小(μm)  颗粒密度（g/ml)  每毫升 载量 建议流速4m/h  pH 稳定性 CIP操作 稳定性暂时
17-5075-05 17-5075-01 STREAMLINE Q XL 100 ml 300 ml 100-300 1.2 > 110 mg BSA/ml 300-500 2-12 2-14
17-5076-05 17-5076-01 STREAMLINE SP XL 100 ml 300 ml 100-300 1.2 > 140 mg 溶菌酶/ml 300-500 4-13 3-14
17-0993-05 17-0993-01 17-0993-02 STREAMLINE SP 100 ml 300 ml 7.5 L 100-300 1.2 60 mg 溶菌酶/ml 300-500 3-13 3-14
17-0994-05 17-0994-01 17-0994-02 STREAMLINE DEAE 100 ml 300 ml 7.5 L 100-300 1.2 40 mg BSA/ml 200-400 2-13 2-14
二］亲和
17-1280-01 STREAMLINE chelating 300 ml 100-300 1.2 40 μ molCu2+/ml 300 3-13 3-14
17-1284-06 17-1284-01 STREAMLINE Heparin 75 ml 300 ml 100-300 1.2 300 4-12 4-13
17-1281-01 17-1281-02 STREAMLINE r-Protein A 75 ml 300 ml 80-165 1.3 50 mg human IgG/ml 200-400 3-10 2-11
三］疏水
17-5121-01 17-5121-02 17-5121-03 STREAMLINE Phenyl 100 ml 300 ml 1 L 100-300 1.2 200-400 3-13 2-13
四］参数测定介质
17-5104-01 17-5104-02 STREAMLINE QUARTZ BASE 300 ml 7.5 L 100-300 1.2 – 200-300 ligand dependent

GE离子交换填料

发表于由

GE离子交换填料

货号 名称
17051010 Q SEPHAROSE FAST FLOW, 25 ML
17070910 DEAE SEPHAROSE FAST FLOW, 25 ML
17071910 CM SEPHAROSE FAST FLOW, 25 ML
17072910 SP SEPHAROSE FAST FLOW, 25 ML
17546810 Capto SP ImpRes, 25 mL
17547010 Capto Q ImpRes, 25 mL
17531610 CAPTO Q, 25 ML
17544110 CAPTO S, 25 ML
17371701 Capto S ImpAct, 25 ml
17128710 ANX SEPHAROSE FF HIGHSUB 25 ML
17101401 Q SEPHAROSE HIGH PERF. 75 ML
17108701 SP SEPHAROSE HIGH PERF. 75 ML
17531602 Capto Q 100 ml
17544101 CAPTO S, 100 ML
17544301 CAPTO DEAE, 100 ML
17094720 SOURCE 15Q, 10 ML
17094410 SOURCE 15S, 10 ML
17050001 DEAE-SEPHACEL 500 ML
17546802 Capto SP ImpRes, 100 mL
17547002 Capto Q ImpRes, 100 mL
17022001 CM-SEPHADEX C-50 100 G
17017001 DEAE-SEPHADEX A-25 100 G
17019001 QAE-SEPHADEX A-25 100 G
17020001 QAE-SEPHADEX A-50 100 G
17021001 CM-SEPHADEX C-25 100 G
17023001 SP-SEPHADEX C-25 100 G
17018001 DEAE-SEPHADEX A-50 100 G
17051001 Q SEPHAROSE FAST FLOW 300 ML
17072901 SP SEPHAROSE FAST FLOW, 300 ML
17371702 Capto S ImpAct, 100 ml
17070901 DEAE SEPHAROSE FAST FLOW 500 ML
17071901 CM SEPHAROSE FAST FLOW 500 ML
17507201 Q SEPHAROSE XL, 300 ML
17507301 SP SEPHAROSE XL, 300 ML
17128701 ANX SEPH 4 FF HIGH SUB 500ML
17094401 SOURCE 15S, 50 ML
17094701 SOURCE 15Q, 50 ML
17071001 DEAE-SEPHAROSE CL-6B 500 ML
17127301 SOURCE 30S, 50 ML
17127501 SOURCE 30Q, 50 ML
17017002 DEAE-SEPHADEX A-25 500 G
17018002 DEAE-SEPHADEX A-50 500 G
17021002 CM-SEPHADEX C-25 500 G
17022002 CM-SEPHADEX C-50 500 G

GE 凝胶过滤填料

发表于由

GE 凝胶过滤填料

货号 名称
17009010 SEPHADEX LH-20, 25 G
17058410 SEPHACRYL S-200 HR, 150 ML
17059910 SEPHACRYL S-300 HR, 150 ML
17060910 SEPHACRYL S-400 HR, 150 ML
17061210 SEPHACRYL S-100 HR, 150 ML
17061310 SEPHACRYL S-500 HR, 150 ML
17001001 SEPHADEX G-10 100 G
17003301 SEPHADEX G-25 MEDIUM 100 G
17003401 SEPHADEX G-25 COARSE 100 G
17003201 SEPHADEX G-25 FINE 100 G
17004201 SEPHADEX G-50 FINE 100 G
17002001 SEPHADEX G-15 100 G
17004301 SEPHADEX G-50 MEDIUM 100 G
17003101 SEPHADEX G-25 SUPERFINE 100 G
17004101 SEPHADEX G-50 SUPERFINE 100 G
17009001 SEPHADEX LH-20 100 G
17005101 SEPHADEX G-75 SUPERFINE 100 G
17048901 SUPEROSE 6 PREP GRADE 125 ML
17053601 SUPEROSE 12 PREP GRADE 125 ML
17006101 SEPHADEX G-100 SUPERFINE 100 G
17058401 SEPHACRYL S-200 HR 750 ML
17059901 SEPHACRYL S-300 HR, 750 ML
17060901 SEPHACRYL S-400 HR, 750ML
17061201 SEPHACRYL S-100 HR 750 ML
17061301 SEPHACRYL S-500 HR 750 ML
17090501 SUPERDEX 30 PREP GRADE 150 ML
17104301 SUPERDEX 200 PREP GRADE 150 ML
17104401 SUPERDEX 75 PREP GRADE 150 ML
17011001 SEPHAROSE 6B 1 L
17012001 SEPHAROSE 4B 1 L
17015001 SEPHAROSE CL-4B 1 L
17016001 SEPHAROSE CL-6B 1 L
17001002 SEPHADEX G-10 500 G
17003402 SEPHADEX G-25 COARSE 500 G
17003302 SEPHADEX G-25 MEDIUM 500 G
17014001 SEPHAROSE CL-2B 1 L
17003202 SEPHADEX G-25 FINE 500 G
17004202 SEPHADEX G-50 FINE 500 G
17004302 SEPHADEX G-50 MEDIUM 500 G
17009002 SEPHADEX LH-20 500 G

GE亲和层析抗体纯化填料

发表于由

GE亲和层析抗体纯化填料

货号 名称
17547806 Capto L, 5 mL
17513801 RMP PROT A SEPH FF 5 ML
17088501 GAMMABIND G SEPHAROSE, 5 ML
17519901 MABSELECT 25 ML
17547801 Capto L, 25 mL
17096303 PROTEIN A SEPHAROSE CL-4B, 25 ML
17528004 NPROTEIN A SEPHAROSE 4FF 25ML
17127902 RPROTEIN A SEPHAROSE FF 25 ML
17061802 PROTEIN G SEPHAROSE 4 FF, 25 ML
17088602 GAMMABIND PLUS SEPHAROSE, 25 ML
17088502 GAMMABIND G SEPHAROSE, 25 ML
17513802 RMP PROT A SEPH FF 25 ML
17543801 MABSELECT SURE 25 ML
17547401 MabSelect SuRe LX 25 ML
17526907 MABSELECT XTRA 25ML
90100093 PROTEIN G SEPHAROSE HP, 50 ML
17519902 MABSELECT 200 ML
17127903 R-PROTEIN A SEPHAROSE FF, 200 ML
17528002 NPROTEIN A SEPH 4 FF 200 ML
17513803 RMP PROT A SEP FF 200 ML
17547802 Capto L, 200 mL
17061805 PROTEIN G SEPHAROSE 4FF 200 ML
17543802 MABSELECT SURE 200 ML
17547402 MabSelect SuRe LX 200 ML
17526902 MABSELECT XTRA 200 ML
90100020 PROTEIN G SEPHAROSE HP, 200 ML
17088504 GAMMABIND G SEPHAROSE, 250 ML
17096302 PROTEIN A SEPHAROSE CL-4B 500ML
17088604 GAMMABIND PLUS SEPHAROSE, 500 ML

GE 亲和层析标签蛋白纯化填料

发表于由

GE 亲和层析标签蛋白纯化填料

货号 名称
28957499 TALON Superflow 10ml
17092107 IMAC SEPHAROSE 6 FF, 25 ML
17531801 NI SEPHAROSE 6FF, 25 ML
17526801 NI SEPHAROSE HP 25 ML
17371201 Ni Sepharose excel 25 ml
17057501 CHELATING SEPHAROSE FAST FLOW
17513201 GLUTATHIONE SEPHAROSE 4FF,25ML
17527901 GLUTATHIONE SEPHAROSE HP 25 ML
28957502 TALON Superflow 50 ml
28935599 StrepTactin Sepharose High Performance 10ml
17092108 IMAC SEPHAROSE 6 FF, 100 ML
28935597 Dextrin Sepharose High Performance 25 ml
17531802 NI SEPHAROSE 6FF, 100 ML
17092007 IMAC SEPHAROSE HP, 100 ML
17526802 NI SEPHAROSE HP 100 ML
17371202 Ni Sepharose excel 100 ml
90100124 CHELATING SEPHAROSE HP, 50 ML
17075605 GLUTATHIONE SEPHAROSE 4B, 100ML
17513202 GLUTATHIONE SEPH 4 FF, 100 ML
17527902 GLUTATHIONE SEPH HP 100 ML
28935598 Dextrin Sepharose High Performance 100 ml
28935600 StrepTactin Sepharose High Performance 50 ml
17075604 GLUTATHIONE SEPHAROSE 4B, 300 ML
17531803 NI SEPHAROSE 6FF, 500 ML
17371203 Ni Sepharose excel 500ml
17513203 GLUTATHIONE SEPH 4 FF, 500 ML
17526804 NI SEPHAROSE HP, 1 l