Harlan AIN diet formulas

Harlan AIN diet formulas

Harlan Teklad动物饲料

Harlan代理

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These formulas are purified diets with commonly used refined ingredients. These basic formulas are often modified for a specific research purpose, such as altering the fat source, a vitamin or mineral level, or adding a compound. See the FAQ section to learn more about the history of these formulas, AIN-76A, AIN-93G, and AIN-93M.

  • CA.170481 AIN-76A purified diet
  • TD.94045 AIN-93G purified diet
  • TD.94048 AIN-93M purified diet

These formulas (as well as other purified diets) do not contain alfalfa and can be used to reduce background autofluorescence in certain imaging applications. Our Global Rodent Diets are also alfalfa-free and are suitable for imaging work. Please contact us for further information about these formulas or modifications.

Other related product codes:

  • TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased)
  • TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased)
  • TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased)
  • TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil.

Harlan AIN diet formulas

这些配方是含有常用精制成分的纯正饮食。这些基本配方通常是为特定的研究目的而修改的,例如改变脂肪来源、维生素或矿物质水平,或添加一种化合物。见常见问题了解更多关于这些公式的历史的章节,AIN-76A,AIN-93g,和AIN-93m。

  • CA.170481AIN-76A纯饲料
  • TD.94045AIN-93g纯饲料
  • TD.9404893米纯饲料

这些配方(以及其他纯化饲料)不含苜蓿,可用于减少背景自荧光在某些成像应用中。我们的全球啮齿动物饮食也是无苜蓿的,适合成像工作.有关这些公式或修改的进一步信息,请与我们联系。

其他相关产品代码:

  • TD.94096适合辐照的AIN-76A版本(维生素含量增加)
  • TD.97184适合辐照的an-93g版本(维生素含量增加)
  • TD.00102AIN-93m适合辐照的版本(维生素含量增加)
  • TD.95092用玉米油代替大豆油的n-93g改性。

Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

A staple of atherosclerosis research

More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model.

With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.

Formula g/Kg
Casein 195.0
DL-Methionine 3.0
Sucrose 341.46
Corn Starch 150.0
Andydrous Milkfat 210.0
Cholesterol 1.5
Cellulose 50.0
Mineral Mix, AIN-76 (170915) 35.0
Calcium Carbonate 4.0
Vitamin Mix, Teklad (40060) 10.0
Ethoxyquin 0.04

Selected nutrient information1

  % By weight % kcal from
     
Protein 17.3 15.2
Carbohydrate 48.5 42.7
Fat 21.2 42.0
     
kcal/g 4.5  
Cholesterol2 0.2%  
  • Values are calculated from ingredient analysis or manufacturer data.
  • 15% added, 0.05% from fat source.

Critical dietary features of TD.88137 related to atherosclerosis development include:

++Cholesterol (0.2% total cholesterol)

++Total fat (21% by weight; 42% kcal from fat)

++High in saturated fatty acids

(>60% of total fatty acids)

++High sucrose (34% by weight)

Typical fatty acid profile of TD.88137

Typical fatty acid analysis, Mean SD  
% of diet1  
     
Total 20.7 1.5  
Saturated fat 12.8 0.8  
Monounsaturated fat 5.6 0.5  
Polyunsaturated fat 1.0 0.2  
Unknown2 1.3 0.3  
       
Typical fatty acid profile, Mean SD  
% of total fatty acids1  
     
Saturated fat 61.8 2.0  
Monounsaturated fat 27.3 2.1  
Polyunsaturated fat 4.7 0.8  
4:0 2.1 1.1  
6:0 1.5 0.7  
8:0 1.1 0.3  
10:0 2.6 0.5  
12:0 3.3 0.5  
14:0 10.6 0.9  
16:0 28.9 1.3  
16:1 1.5 0.2  
18:0 12.5 0.8  
18:1 (Oleic) 20.9 2.6  
18:1 Isomers3 4.0 1.2  
18:2 (Linoleic) 2.3 1.0  
18:2 Isomers4 1.3 0.5  
18:3 (Linolenic) 0.7 0.2  
  • n = 21, analysis conducted by two independent laboratories.
  • Unidentified fatty acids and those contributing on average less than 0.5% of total fatty acids.
  • Includes trans isomers elaidic and vaccenic acid and unidentified cis isomers.
  • Includes trans isomers.

Key points from the literature

TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:

++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks (2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).

++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic arch (6, 7).

For further information about TD.88137, or if you are interested in learning more about other atherogenic or high fat diets contact us at

With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis, hypertension and metabolic syndrome. Contact us for a more extensive reference list.

Control diet options for TD.88137

Natural ingredient diets

++Also referred to as standard diets or chow

++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared to TD.88137

++Limits inferences to differences in dietary pattern versus a specific dietary component

Ingredient matched, low fat diets

++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients

++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at

Suggested ingredient matched, low fat controls

Diet kcal/g Fat, % by % kcal Fat sources, Sucrose, %
    weight from fat % by weight by weight
        3.7% milk fat,  
TD.05230 3.7 5.2 12.6 1.3% soybean 34.1
        oil  
           
        3.7% milk fat,  
TD.08485 3.6 5.2 13.0 1.3% soybean 12.0
        oil  
           

Additional controls are available. Contact a nutritionist at

Key planning information:

  • Store diet refrigerated and plan to use within six months. Diet should be replaced at minimum once per week when fed on cage tops.
  • Diets available as a soft ½” pellet or as a crumbly powder.
  • Three kg minimum order quantity. For planning purposes, estimates for diet uses (including feed intake and diet waste) are 5 g of diet per mouse and 30 g of diet per rat per day.
  • Two-day lead time for orders less than 10 kg. Two-week lead time for larger quantity orders.
  • Lead time for irradiation adds two weeks for any quantity of diet and must be requested at the time you place your order. Changes in texture and browning may occur with irradiation.
  • Shipping can affect pellet quality. Vacuum packaging can offer protection of the pellets during shipping. Two-day shipping is recommended during warmer months.

Contact us to place an order,

References

  1. Plump, A.S., et al.,Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell, 1992. 71(2): p. 343-53.
  2. Nakashima, Y., et al.,Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994. 14(1): p. 133-40.
  3. Febbraio, M., et al.,Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.

J Clin Invest, 2000. 105(8): p. 1049-56.

  1. Nakashima, Y., et al.,Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb

Vasc Biol, 1998. 18(5): p. 842-51.

  1. Towler, D.A., et al.,Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.

J Biol Chem, 1998. 273(46): p. 30427-34.

  1. Tsuchiya, K., et al.,FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
  2. Huszar, D., et al.,Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
  3. Yang, B., et al.,Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
  4. Schafer, K., et al.,Leptin promotes vascular remodeling and neointimal growth in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
  5. Lijnen, H.R., et al.,Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc Biol, 2003. 23(1): p. 78-84.
  6. Maquoi, E., et al.,Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002. 51(4): p. 1093-101.
  7. VanSaun MN, et al. High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol 175:355-64.
  8. Dixon LJ, et al. Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One 8:e56100.

Harlan Teklad动物饲料分类

Harlan Teklad动物饲料分类

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Standard natural ingredient diets  标准天然成分饮食

  • Rodent diets  啮齿类食物
  • Dog diets  狗粮
  • Rabbit diets   兔饲料
  • Guinea pig diets    豚鼠日粮
  • Primate diets  灵长类饮食
  • Swine diets  猪日粮
  • Other diets

Custom research diets  定制研究饮食

  • Ordering and services  订购和服务
  • NAFLD and NASH    NAFLD和NASH
  • AIN diet formulas   AIN饮食配方
  • Atherogenic    动脉粥样硬化

Atherogenic

Diet can be a useful tool to induce or accelerate atherosclerosis in laboratory animal models. Key dietary features used to induce atherosclerosis in rodents vary depending on the research model, desired endpoint, and length of feeding. While formulations of atherogenic diets continue to evolve, the options that are well-described in the literature are summarized below. For more information on each diet option and literature references see the expandable tabs following the diet table.

Research use Key dietary features Examples
“Western” purified atherogenic diet
Accelerated hypercholesterolemia and plaque formation in genetically modified models such as Apoe and Ldlr deficient mice.

Used for diet induced obesity in a variety of rodent models.

  • High fat diet (20 – 23% by weight; 40 – 45% kcal from fat)
  • Saturated fatty acids (SFA >60% of total fatty acids)
  • Milkfat/butterfat
  • Sucrose (34% by weight)
  • Cholesterol (0.2% total)
TD.88137
TD.10885
“Western” purified atherogenic diet with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

  • High fat diet (15 – 20% by weight; 34 – 45% kcal from fat)
  • Saturated fatty acids (SFA >55% of total fatty acids)
  • Milkfat/butterfat, cocoa butter
  • Sucrose (30-50% by weight)
  • Cholesterol (1 – 1.25%)
  • Cholate Source (0.5%)*
TD.02028
TD.09237
Hybrid high fat diets with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

  • 75% rodent breeder diet; 25% purified ingredients
  • High fat (~15% by weight; 37% kcal from fat)
  • Saturated fatty acids (SFA >45% of total fatty acids)
  • Cholesterol (1.25%)
  • Cholate source (0.5%)*
TD.88051
TD.90221
Standard diets with added cholesterol
Induce hypercholesterolemia in genetically modified and wild type models without promoting obesity.
  • Standard, grain-based rodent diet
  • Minimal/moderate phytoestrogen diets recommended
  • Cholesterol (1 – 4%)
TD.120097
TD.07841
TD.01383

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available.

  • Diet induced obesity    饮食诱导肥胖
  • Fat/lipid adjusted   脂肪/脂调节
  • Carbohydrate adjusted    碳水化合物调节
  • Protein adjusted diets  蛋白质调节饮食
  • Vitamin adjusted diets  维生素调整饮食
  • Mineral adjusted diets  矿物调节饮食
  • NaCl adjusted (natural ingredient)  盐调节(天然成分)
  • Amino acid defined  氨基酸定义
  • Doxycycline diets  多西环素饮食
  • Tamoxifen diets  他莫昔芬饮食
  • Customer supplied ingredients  客户供应的配料
  • Basal mixes  基混合体
  • Isoflavone adjusted  异黄酮调节
  • Rabbit, swine and other species  兔子、猪和其他物种
  • Diet ingredients  饮食成分

Medicated diets  药物饮食

Teklad medicated lab animal diets are manufactured either at a subsidiary plant or at our custom research diet facility. Contact customer service or your local Envigo account representative for pricing and availability of stocked diets. Contact a nutritionist to discuss customized versions of these or other medicated diets.

Medicated diets must be used as directed by a veterinarian.

Stocked medicated rodent diets
Primary Purpose Product Code Medication Concentration Base diet Packaging
Fur mite treatment TD.130755 Ivermectin 12 ppm 2018 25 lb bag- irradiated
Pinworm treatment TD.01432 Fenbendazole 150 ppm 2018S 25 lb bag- irradiated*
50 lb bag- autoclavable
Pneumocystis pneumonia
prevention
TD.06596 Uniprim® 275 ppm trimethoprim
1365 ppm sulfadiazine
2018 25 lb bag- irradiate

Harlan Teklad TD.120240 Doxycycline饲料 40 ppm Doxy Diet (2018, B)

Harlan Teklad TD.120240 Doxycycline饲料 40 ppm Doxy Diet (2018, B)

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.704
Doxycycline Hyclate 0.046
Blue Food Color 0.25

Key Features

  • Standard Diet Base
  • 2018
  • Doxycycline
  • Color Coded Blue

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week  More frequent replacement may be advised
  • Lead time:    2 weeks non-irradiated     4 weeks irradiated

Footnote

Modification of TD.10483 to add blue coloring. Designed to add 40 mg doxycyline/kg diet using the Teklad 2018 Global Rodent Diet as the base. Doxycycline hyclate contains approximately 87% doxycycline.

Selected Nutrient Information1

  % by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Options (Fees Will Apply)

  • Rush order (pending availability)
  • Irradiation (see Product Specific Information)
  • Vacuum packaging (1 and 2 Kg)

Harlan Teklad Medicated diets 药物饲料

Harlan Teklad Medicated diets 药物饲料

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Teklad medicated lab animal diets are manufactured either at a subsidiary plant or at our custom research diet facility. Contact customer service or your local Envigo account representative for pricing and availability of stocked diets. Contact a nutritionist to discuss customized versions of these or other medicated diets.

特克拉德药物实验室动物饮食是在一个附属工厂或在我们的定制研究饮食设施生产。与客户服务部门或当地Envigo客户代表联系,了解库存饮食的定价和供应情况。联系营养师讨论这些或其他药物饮食的定制版本。

Medicated diets must be used as directed by a veterinarian.

Stocked medicated rodent diets
Primary Purpose Product Code Medication Concentration Base diet Packaging
Fur mite treatment TD.130755 Ivermectin 12 ppm 2018 25 lb bag- irradiated
Pinworm treatment TD.01432 Fenbendazole 150 ppm 2018S 25 lb bag- irradiated*
50 lb bag- autoclavable
Pneumocystis pneumonia
prevention
TD.06596 Uniprim® 275 ppm trimethoprim
1365 ppm sulfadiazine
2018 25 lb bag- irradiated*
COLLAPSE ALL TABS

  • Testing shows very little medication loss due to manufacturing
  • Recommended storage at or below 70 degrees fahrenheit with humidity at or below 65%
  • Durable cylindrical pellets are visually distinct from flattened oval or extruded pellets (see picture)
  • 2018 supports growth and reproduction
  • 2018 is closely related to other Global Rodent Diets, and shares some commonality with traditional rodent diets

The anthelmintic ivermectin is used for the treatment of fur mites. TD.130755 (Irradiated 2018, 12 ppm Ivermectin) is stocked in 25 lb bags. Contact customer service regarding availability of TD.130755.

The anthelmintic fenbendazole is used for treatment of pinworms. TD.01432 (2018S, 150 ppm fenbendazole) is stocked in 25 lb bags that are irradiated or in 50 lb bags that can be autoclaved (note autoclaving reduces fenbendazole concentration and is only recommended if using to treat mice). Contact customer service regarding availability ofTD.01432.

Uniprim® contains trimethoprim and sulfadiazine, used to prevent occurrences of Pneumocystis pneumonia in immunocompromised mice and rats. TD.06596 (Irradiated 2018, 4100 ppm Uniprim®) is stocked in 25 lb bags. Contact customer service regarding availability of TD.06596.

Custom diets with Ivermectin, Uniprim®, and Fenbendazole are available. Modifications include: different base diet, coloring with food dyes, and other dosing concentrations based on your unique requirements. Contact a nutritionist to discuss your needs.

Other medications and compounds such as doxycycline and tamoxifen may be added to diets at our custom research diet facility. Visit our custom research diets site or contact us

药物饮食必须按照兽医的指示使用。

含药啮齿动物饲料
主要目的 产品代码 药物治疗 浓度 基础饮食 包装
毛螨处理 TD.130755 伊维菌素 百万分之十二 2018 25磅包-辐照
粉螨治疗 TD.01432 芬苯达唑 百万分之150 2008 S 25磅包-辐照*
50磅包-高压釜
肺孢子虫p肺炎
预防
TD.06596 Uniprim® 275 ppm甲氧苄啶
1365 ppm磺胺嘧啶
2018 25磅包-辐照*
COLLAPSE ALL TABS

  • 测试显示制造过程中药物损失很小。
  • 建议贮存温度在华氏70度或以下,湿度在65%或以下
  • 耐用的圆柱形球团在视觉上有别于扁平的椭圆形或挤压球团(见图)
  • 2018支持生长和繁殖
  • 2018与其他全球啮齿动物饮食密切相关,并与传统的啮齿动物饮食有一些共同之处

这个驱虫药伊维菌素用于治疗毛皮螨。TD.130755(辐照2018年,12 ppm伊维菌素)储存在25磅袋。与客户服务部门联系,了解TD.130755.

本发明为驱虫药芬苯达唑。TD.01432(2018S,150 ppm芬苯达唑)储存在25磅经辐照的袋子中,或装在50磅可被压的袋子中(注意,高压灭菌可以降低芬苯达唑的浓度,只有当用于治疗小鼠时才推荐使用)。与客户服务部门联系,了解TD.01432.

Uniprim®含有甲氧苄啶和磺胺嘧啶,用于预防免疫受损小鼠和大鼠肺炎肺孢子虫肺炎的发生。TD.06596(2018年辐照,4100 ppm Uniprim)®)装在25磅重的袋子里。与客户服务部门联系,了解TD.06596.

伊维菌素定制饮食®,芬苯达唑是可行的。修改包括:不同的基础饮食,染色与食物染料,以及其他剂量的浓度,根据您的独特要求。联系营养师来讨论你的需求。

其他药物和化合物,如强力霉素和他莫昔芬可以添加到我们的定制研究饮食设施的饮食。访问我们的定制研究饮食网站或联系我们以获得更多信息。

Harlan 强力霉素饲料 Doxycycline diets

Harlan 强力霉素饲料 Doxycycline diets

Harlan代理,Harlan饲料代理,欢迎访问Harlan官网或者咨询我们获取更多相关产品信息。

Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1 – 2 days (non-irradiated) or 1 – 2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don’t find a suitable option below.

EnvigoTeclad饮食使各种多西环素包含饮食为您的Tet调节系统。TD.01306以625毫克/千克的强力霉素添加到2018年全球啮齿动物饲料中,通常可在1至2天(未辐照)或1-2周(辐照)内装运。饮食很容易定制。定制规格包括:基础饮食,多西环素浓度和食用色素。联系我们如果你在下面找不到合适的选择的话,来讨论你的需求。

Formula examples with Teklad global 2018 base diet
Doxycycline hyclate1 USP (mg/kg diet) Natural color Alternatives with food coloring
mg per kg diet
46 TD.10483 TD.180327 Red
TD.120240 Blue
100 TD.04233 TD.130840 Blue
200 TD.00502 TD.180625 Red
TD.150431 Blue
TD.04104 Green
625 TD.01306
stocked2
TD.08541 Red
TD.120769 Blue
TD.09628 Green
1000 TD.05298 TD.06294 Red
TD.120658 Blue
2000 TD.05512 TD.140011 Red
TD.09633 Green
6000 TD.01533

1 Doxycycline hyclate is ~87% doxycycline
2 TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Considerations when choosing a base diet

    • Teklad Global Rodent Diets® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
    • Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.
Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate1 at 625 mg/kg diet
Base diet Natural color Alternatives with food coloring
mg per kg diet
2014 TD.00426 TD.07382 Red
TD.07573 Yellow
2016 TD.02503 TD.140263 Blue
TD.170364 Red
2019 TD.09651 TD.09761 Green
2020 TD.110720 TD.130141 Red

1 Doxycycline hyclate is ~87% doxycycline

Advantages of diet delivery of doxycycline

  • Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
  • Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
  • Diet is typically changed once per week. Water may need to be changed every few days
  • Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

Key planning information

  • Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
    • TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation
  • Irradiation (20 – 50 kGy) is optional, and must be requested at time of order

Safe handling of doxycycline diets

  • Doxycycline is a tetracycline class antibiotic.
  • Usual dose for therapeutic effects in humans is 100-200 mg/day. Doxycycline diets for rodents typically contain ~2 mg/pellet.
  • Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

多西环素饮食给药的优势

  • 典型的多西环素浓度可以用于饮食和水。低浓度的多西环素可能足够,625毫克/千克的饮食是最常见的浓度。
  • 饮食能保护身体免受光线伤害。水可能必须用深色或铝箔包装的瓶子浇水。
  • 饮食通常每周改变一次。水可能需要每隔几天更换一次。
  • 饮食供应也可以减少脱水的风险,并避免需要给予蔗糖(考索恩等)。艾尔。(2007年)

关键规划信息

  • 最小订货量为3公斤,足以喂养20只小鼠一个月。
  • 储存冷冻饮食,并计划在六个月内使用。
  • 典型的准备时间为两周(如果经过辐照,则为四周)
    • TD.01306通常在未经辐照的2天内或在辐照的2周内装运。
  • 辐射(20-50 kGy)是可选的,必须按顺序要求。

多西环素饮食的安全处理

  • 强力霉素是四环素类抗生素。
  • 人体治疗效果的一般剂量为每天100至200毫克。用于啮齿动物的多西环素饮食通常含有~2毫克/粒。
  • 当处理饮食时,使用典型的实验室预防措施,如实验室外套、手套和口罩,可以将多西环素意外暴露量降到最低。
  • 您的化学安全部门应联系,以获得额外的机构特定的指导方针,以处理和处置多西环素的饮食。

Harlan TD.95027低钙饲料(0.4%P)

Harlan TD.95027低钙饲料(0.4%P)

Harlan货号:TD.95027

英文名称:Low Calcium Diet (0.4% P)

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula                                                                     g/Kg

Casein                                                                    200.0

Cystine                                                                     3.0

Sucrose                                                            342.188

Corn Starch                                                         320.0

Soybean Oil                                                          60.0

Cellulose                                                              40.0

Mineral Mix, Ca-P Deficient (79055)                    13.37

Potassium Phosphate, monobasic                     11.43

Vitamin Mix, Teklad (40060)                                10.0

Ethoxyquin, antioxidant                                       0.012

Key Features

+ Purified Diet

+ Calcium

+ Phosphorus

Key Planning Information

+ Products are made fresh to order

+Store product at 4°C or lower

+Use within 6 months (applicable to most diets)

+Box labeled with product name, manufacturing date, and lot number
+ Replace diet at minimum once per week More frequent replacement may be advised
+ Lead time:
· 2 weeks non-irradiated
· 4 weeks irradiated

上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫诊断试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

上海金畔生物科技有限公司

服务热线:18301939375   QQ:3258089810    3259632176

Email:  info@jinpanbio.com

官网:http://www.jinpanbio.com

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Atherogenic

Research use Key dietary features Examples
“Western” purified atherogenic diet
Accelerated hypercholesterolemia and plaque formation in genetically modified models such as Apoe and Ldlr deficient mice.

Used for diet induced obesity in a variety of rodent models.

High fat diet (20 – 23% by weight; 40 – 45% kcal from fat)

Saturated fatty acids (SFA >60% of total fatty acids)

Milkfat/butterfat

Sucrose (34% by weight)

Cholesterol (0.2% total)

TD.88137
TD.10885
“Western” purified atherogenic diet with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

High fat diet (15 – 20% by weight; 34 – 45% kcal from fat)

Saturated fatty acids (SFA >55% of total fatty acids)

Milkfat/butterfat, cocoa butter

Sucrose (30-50% by weight)

Cholesterol (1 – 1.25%)

Cholate Source (0.5%)*

TD.02028
TD.09237
Hybrid high fat diets with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

75% rodent breeder diet; 25% purified ingredients

High fat (~15% by weight; 37% kcal from fat)

Saturated fatty acids (SFA >45% of total fatty acids)

Cholesterol (1.25%)

Cholate source (0.5%)*

TD.88051
TD.90221
Standard diets with added cholesterol
Induce hypercholesterolemia in genetically modified and wild type models without promoting obesity. Standard, grain-based rodent diet

Minimal/moderate phytoestrogen diets recommended

Cholesterol (1 – 4%)

TD.120097
TD.07841
TD.01383

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.

在实验动物模型中,饮食是诱导或加速动脉粥样硬化的有用工具。用于诱发啮齿类动物动脉粥样硬化的主要饮食特征因研究模型、所需终点和喂养时间的不同而不同。尽管动脉粥样硬化饮食的配方在不断发展,但文献中详细描述的备选方案概述如下。有关每个饮食选项和文献参考的更多信息,请参见节目表后面的可扩展选项卡。

研究用途 主要饮食特征 实例
“西方”纯正致动脉粥样硬化饮食
加速高胆固醇血症和斑块形成的转基因模型,如APOE和Ldlr缺乏的小鼠。

用于饮食诱导肥胖的各种啮齿动物模型。

  • 高脂饮食(按体重计算占20-23%;脂肪占40-45%)
  • 饱和脂肪酸(SFA>总脂肪酸的60%)
  • 乳脂/乳脂
  • 蔗糖(按重量计占34%)
  • 胆固醇(总数0.2%)
TD.88137
TD.10885
添加胆固醇和胆酸来源的“西方”纯动脉粥样硬化饮食*
在野生型小鼠和大鼠中诱导高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

  • 高脂饮食(体重15-20%;脂肪34-45千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的55%)
  • 乳脂/黄油、可可脂
  • 蔗糖(重量占30%-50%)
  • 胆固醇(1-1.25%)
  • 巧克力来源(0.5%)*
TD.02028
TD.09237
添加胆固醇和胆酸盐的混合高脂饮食*
在野生型小鼠和大鼠中诱导高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

也用于造石(胆石)啮齿动物的研究。

  • 75%的啮齿动物饲养员;25%的纯化成分
  • 高脂肪(体重约15%;脂肪37%千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的45%)
  • 胆固醇(1.25%)
  • 巧克力源(0.5%)*
TD.88051
TD.90221
添加胆固醇的标准饮食
在转基因和野生型模型中诱导高胆固醇血症而不促进肥胖。
  • 标准的、以谷物为基础的啮齿动物饮食
  • 最小/中度植物雌激素饮食建议
  • 胆固醇(1-4%)
TD.120097
TD.07841
TD.01383

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。

“Western” purified atherogenic diet

“Western” style diets are fed to genetically-modified cardiovascular models, such as Apoe and Ldlr deficient mice, to accelerate and enhance hypercholesterolemia and plaque formation and to elicit phenotypes commonly associated with metabolic syndrome.Within the atherogenic literature, a “Western” diet typically is described as a purified rodent diet with 20-23% milkfat/butterfat, 0.2% total cholesterol, and 34% sucrose by weight.TD.88137is an example of a “Western” style diet that was originally designed to characterize and enhance atherosclerosis development in a newly generated Apoe-deficient mouse model.Contact us for more information about “Western” style diets, modifications, or possible control diets.

Examples:

TD.88137Adjusted calories diet (42% from fat, 0.2% total cholesterol)

TD.1088545% fat Kcal diet (0.2% total cholesterol)

Research use:

Accelerated hypercholesterolemia and plaque formation in genetically-modified models, such as Apoe and Ldlr deficient mice.

Used for diet-induced obesity in a variety of rodent models.

Key dietary features:

High Fat Diet (20-23% by weight; 40 – 45% kcal from fat)

Saturated fatty acids (SFA >60% of total fatty acids)

Milkfat/butterfat

Sucrose (34% by weight)

Cholesterol (0.2% total)

References:

1.Febbraio, M., et al., Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.J Clin Invest, 2000.105(8): p.1049-56.

2.Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1.Arterioscler Thromb Vasc Biol, 2000.20(4): p.1068-73.

3.Nakashima, Y., et al., ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree.Arterioscler Thromb, 1994.14(1): p.133-40.

4.Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse.Arterioscler Thromb Vasc Biol, 1998.18(5): p.842-51.

5.Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells.Cell, 1992.71(2): p.343-53.

6.Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.J Biol Chem, 1998.273(46): p.30427-34.

7,Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis.Cell Metab, 2012.15(3): p.372-81.

“Western” purified atherogenic diet with added cholesterol and cholate source*

Wild type mice and rats generally are resistant to atherosclerosis, requiring more extreme dietary manipulation to modify lipoprotein profiles and develop mild atherosclerosis (foam cells, fatty streaks).Modern formulations are made completely of purified ingredients because this more refined approach has been reported to decrease the incidence of gallstones and liver damage associated with less refined and more traditional dietary approaches.To induce mild atherosclerosis in wild type animals, the “Western” purified diet can be modified to increase cholesterol (1-1.25%) and add a bile salt such as sodium cholate or cholic acid.Contact us for more information, modifications, or possible control diets.

Examples of purified high fat diets with added cholesterol and cholate source*:

TD.0202821% milkfat (1.25% cholesterol, 0.5% cholic acid)

TD.0923715% milkfat diet (1% cholesterol, 0.5% sodium cholate)

Examples of purified high fat diets with added cholesterol (without cholate source):

TD.9612121% milkfat (1.25% cholesterol)

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Key dietary features:

High fat diet (15-20% by weight; 34 – 45% kcal from fat)

Saturated fatty acids (SFA >55% of total fatty acids)

Milkfat/butterfat, cocoa butter

Sucrose (30-50% by weight)

Cholesterol (1 – 1.25%)

Cholate source (0.5%)*

References:

1.Bernal, C., et al., Lipid biomarkers and metabolic effects of lycopene from tomato juice on liver of rats with induced hepatic steatosis.J Nutr Biochem, 2013.24(11): p.1870-81.

2.Gao, Q., et al., Atherogenic diets exacerbate colitis in mice deficient in glutathione peroxidase.Inflamm Bowel Dis, 2010.16(12): p.2043-54.

3.Lichtman, A.H., et al., Hyperlipidemia and atherosclerotic lesion development in LDL receptor-deficient mice fed defined semipurified diets with and without cholate.Arterioscler Thromb Vasc Biol, 1999.19(8): p.1938-44.

4.Marcondes, M.C., et al., Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta.Brain Behav Immun, 2011.25(8): p.1649-57.

5.Nishina, P.M., et al., Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice.J Lipid Res, 1993.34(8): p.1413-22.

6.Nishina, P.M., et al., Atherosclerosis and plasma and liver lipids in nine inbred strains of mice.Lipids, 1993.28(7): p.599-605.

7.Yue, P., et al., Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice.PLoS One, 2010.5(3): p.e9906.

8.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.

Hybrid high fat diets with added cholesterol and cholate source*

Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet.The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet).The resulting mixture recreated inTD.88051/TD.90221(same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate.This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage.Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases.Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats.Contact us for more information, modifications, or possible control diets.

Examples of hybrid high-fat diets with added cholesterol and cholate source*:

TD.88051andTD.90221(same formula) are Teklad product codes for hybrid atherogenic diets

Example of hybrid high-fat diet with added cholesterol (without cholate source):

TD.94059

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

Key dietary features:

75% rodent breeder diet; 25% purified ingredients

High fat (~15% by weight; 37% kcal from fat)

Saturated fatty acids (SFA >45% of total fatty acids)

Cholesterol (1.25%)

Cholate source (0.5%)*

References:

1.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.

2.Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis.J Lipid Res, 2000.41(4): p.521-31.

3.George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65.Arterioscler Thromb Vasc Biol, 1999.19(3): p.505-10.

4.Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice.Arterioscler Thromb Vasc Biol, 2002.22(1): p.121-6.

5.Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice.Atherosclerosis, 1987.68(3): p.231-40.

6.Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis.Atherosclerosis, 1998.136(1): p.17-24.

7.Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression.J Biol Chem, 2003.278(44): p.42774-84.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.

Standard diets with added cholesterol

Standard, natural ingredient diets with cholesterol added are fed to induce hypercholesterolemia.Various levels of cholesterol, fat, and/or bile salts can be added to one of the numerous standard rodent diets stocked by Envigo Teklad.For many applications, adding these components to Envigo’s minimal-to-moderate phytoestrogen global rodent diets is recommended.Our minimal phytoestrogen global rodent diets are soybean meal free, limiting the effect of phytoestrogens on your research outcomes.Soybean meal, a common dietary source of phytoestrogens, has been shown to decrease aortic fatty streak development and modify plasma cholesterol, which may reduce the risk of developing atherosclerosis.Limiting dietary soybean meal may reduce confounding variables within your dietary-induced atherosclerosis model.Contact a nutritionist to discuss additional diet options.

Examples of minimal and moderate phytoestrogen rodent diets with added cholesterol:

TD.1200971% cholesterol diet (2020 – minimal phytoestrogens)

TD.078412% cholesterol diet (2016 – minimal phytoestrogens)

TD.013832% cholesterol (2018 – Moderate phytoestrogens)

Research use:

Induce hypercholesterolemia in genetically-modified and wild type models without promoting obesity.

Key dietary features:

Standard, grain-based rodent diet

Minimal/moderate phytoestrogen diets recommended

Cholesterol (1 – 4%)

References:

1.Belch, J.J., et al., Longitudinal assessment of endothelial function in the microvasculature of mice in-vivo.Microvasc Res, 2013.85: p.86-92.

2.Hartvigsen, K., et al., A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome.Arterioscler Thromb Vasc Biol, 2007.27(4): p.878-85.

Diets for additional animal models of atherosclerosis

Rabbits, hamsters, and swine are common models of atherosclerosis.Contact a nutritionist for information and formula examples.Seerabbit, swine and other speciesfor information and formula examples.

“西方”纯正致动脉粥样硬化饮食

“西式”饮食被喂给基因修饰的心血管模型,如APOE和Ldlr缺乏的小鼠,以加速和促进高胆固醇血症和斑块形成,并引出通常与代谢综合征相关的表型。在动脉粥样硬化的文献中,“西方”饮食通常被描述为含有20-23%乳脂/蝴蝶脂肪、0.2%总胆固醇和34%蔗糖的纯啮齿动物饮食。TD.88137这是一种“西式”饮食的例子,最初的目的是在一种新生成的APOE缺陷小鼠模型中描述和促进动脉粥样硬化的发展。联系我们,以获得更多关于“西方”风格的饮食,修改,或可能的控制饮食。

例子:

  • TD.88137调整卡路里饮食(42%来自脂肪,0.2%总胆固醇)
  • TD.1088545%脂肪KCAL饮食(总胆固醇0.2%)

研究用途:

加速高胆固醇血症和斑块形成的转基因模型,如APOE和Ldlr缺乏的小鼠。

用于饮食诱导肥胖的各种啮齿动物模型。

主要饮食特征:

  • 高脂肪饮食(按体重计算占20-23%;脂肪占40-45%)
  • 饱和脂肪酸(SFA>总脂肪酸的60%)
  • 乳脂/乳脂
  • 蔗糖(按重量计占34%)
  • 胆固醇(总数0.2%)

野生型小鼠和大鼠一般对动脉粥样硬化有抵抗力,需要更极端的饮食操作来改变脂蛋白谱,形成轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。现代配方完全是由纯化成分制成的,因为据报道,这种更精细的方法可以减少较少精制和较传统饮食方法引起的胆结石和肝脏损害的发生率。为了在野生型动物中诱导轻度动脉粥样硬化,可对“西方”纯化饲料进行改良,以提高胆固醇(1-1.25%),并添加胆酸钠或胆酸等胆盐。联系我们获得更多的信息,修改,或可能的控制饮食。

添加胆固醇和胆酸盐来源的纯化高脂饮食的例子*:

  • TD.0202821%乳脂(1.25%胆固醇,0.5%胆酸)
  • TD.0923715%乳脂饮食(1%胆固醇,0.5%胆酸钠)

添加胆固醇的纯化高脂饮食的例子(没有胆酸来源):

  • TD.9612121%乳脂(1.25%胆固醇)

研究用途:

主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

主要饮食特征:

  • 高脂饮食(体重15-20%;脂肪34-45千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的55%)
  • 乳脂/黄油、可可脂
  • 蔗糖(重量占30%-50%)
  • 胆固醇(1-1.25%)
  • 巧克力源(0.5%)*

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系,了解其他选项。

贝弗利·派根和他的同事首先通过喂养一种混合性动脉粥样硬化饮食来描述C57BL/6小鼠动脉粥样硬化的发展。这种混合饲料是通过将一种天然成分的老鼠饲料按3:1的比例与浓缩的纯化饲料(含5%胆固醇和2%胆酸钠;称为Thoms-Hartroft饮食)混合而成的。产生的混合物在TD.88051/TD.90221(同配方)含有~15.8%脂肪、1.25%胆固醇和0.5%胆酸钠。这组人后来将混合动脉粥样硬化饮食方法与更现代的“西方”纯化动脉粥样硬化饮食方法进行了比较,并添加了胆固醇和胆酸盐,发现混合动脉粥样硬化饮食会导致更多的胆结石和肝脏损伤。混合饮食含有多种未精制的成分,这些成分可能会改变血脂代谢和动脉粥样硬化的发生,并且不允许对成分和营养物进行精确控制,以用于慢性病的研究。虽然已经开发出了更精细的饮食,但混合动脉粥样硬化饮食仍然是野生型小鼠和大鼠诱发轻度动脉粥样硬化和胆结石的流行食品。联系我们获得更多的信息,修改,或可能的控制饮食。

添加胆固醇和胆酸盐的混合高脂饮食实例*:

  • TD.88051和TD.90221(相同的配方)是特克拉德混合动脉粥样硬化饮食的产品代码。

添加胆固醇的混合高脂饮食的例子(没有胆酸来源):

  • TD.94059

研究用途:

主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

也用于造石(胆石)啮齿动物的研究。

主要饮食特征:

  • 75%的啮齿动物饲养员;25%的纯化成分
  • 高脂肪(体重约15%;脂肪37%千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的45%)
  • 胆固醇(1.25%)
  • 巧克力源(0.5%)*

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系,了解其他选项。

标准的,添加胆固醇的天然成分饮食是用来诱导高胆固醇血症的。不同水平的胆固醇,脂肪和/或胆汁酸盐可以添加到众多的标准啮齿动物饮食之一,由EnvigoTeclad。在许多应用中,建议将这些成分添加到Envigo的最小到中等植物雌激素的全球啮齿动物饮食中。我们最小的植物雌激素全球啮齿动物饮食是不含豆粕的,限制了植物雌激素对你的研究结果的影响。豆粕是一种常见的植物雌激素来源,它可以减少主动脉脂肪条纹的发育,改善血浆胆固醇,从而降低动脉粥样硬化的风险。限制饮食豆粕可能会减少饮食诱导动脉粥样硬化模型中的混杂变量。联系营养学家讨论其他饮食选择。

添加胆固醇的最小和中度植物雌激素啮齿动物饮食的例子:

  • TD.120097  1%胆固醇饮食(2020年-最小植物雌激素)
  • TD.07841  2%胆固醇饮食(2016年-最小植物雌激素)
  • TD.01383  2%胆固醇(2018年-中度植物雌激素)

研究用途:

在转基因和野生型模型中诱导高胆固醇血症而不促进肥胖。

主要饮食特征:

  • 标准的、以谷物为基础的啮齿动物饮食
  • 最小/中度植物雌激素饮食建议
  • 胆固醇(1-4%)

兔子、仓鼠和猪是动脉粥样硬化的常见模型。请与营养师联系,了解有关信息和公式示例。看见兔子、猪和其他物种有关信息和公式示例。

Harlan蛋白质调节饲料 Protein adjusted diets

Harlan蛋白质调节饲料 Protein adjusted diets

Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Protein adjusted diets

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan蛋白质调节饲料 Protein adjusted diets

Protein adjusted diets | Envigo

A few protein adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the protein type or content of a diet.

Formula examples:

TD.93328      “Protein free” diet

TD.90016      6% Protein diet

TD.91352       20% Protein diet

TD.90018      40% Protein diet

These diet examples are from a series of diets with the following features: casein-based, isocaloric (3.8 kcal/g) and matched for fat (5.5%), calcium (0.7%), and phosphorus (0.54%).  There are other diets available from this series of diets, as well as other unique protein adjusted diets.

Casein is the most widely used refined protein source, but it is also possible to use other refined protein sources, such as “vitamin-free” test casein (alcohol extracted), lactalbumin, isolated soy protein, and egg white solids. These particular protein sources are usually used to achieve a specific research objective, rather than for routine use. For instance, vitamin-free test casein is most appropriately used in vitamin deficient diets, and egg white solids is most suitable for zinc or biotin deficient diets.

Adjusted protein diets usually range from “protein-free” (trace) to 60% protein, depending on the research.

一些蛋白质调整的公式如下所示。请与我们联系,以获得更多的这种性质的配方,或更多有关改变蛋白质类型或饮食内容的信息。

公式示例:

TD.93328“无蛋白质”饮食

TD.90016  6%蛋白质饮食

TD.91352  20%蛋白质饮食

TD.90018  40%蛋白质饮食

这些饮食的例子来自一系列的饮食,有以下特点:酪蛋白为基础,等热量(3.8千卡/克),与脂肪(5.5%),钙(0.7%)和磷(0.54%)匹配。从这一系列的饮食中可以得到其他的饮食,以及其他独特的蛋白质调整的饮食。

酪蛋白是最广泛使用的精制蛋白质来源,但也可以使用其他精制蛋白质来源,如“无维生素”测试酪蛋白(醇提),乳清蛋白,分离大豆蛋白和蛋清固体。这些特定的蛋白质来源通常用于实现特定的研究目标,而不是用于常规用途。例如,无维生素测试酪蛋白最适合用于缺乏维生素的饮食,而蛋清固体最适合于锌或生物素缺乏的饮食。

根据研究结果,调整后的蛋白质饮食通常从“无蛋白质”(微量)到60%的蛋白质。

上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫诊断试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

上海金畔生物科技有限公司

服务热线:18301939375   QQ:3258089810    3259632176

Email:  info@jinpanbio.com

官网:http://www.jinpanbio.com

Harlan 维生素调整饲料 Vitamin adjusted diets

Harlan 维生素调整饲料 Vitamin adjusted diets

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

A few vitamin adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the vitamin profile of a diet, and please read below to learn more.

Formula Examples:

TD.86143 Vitamin A deficient diet

TD.89123 Vitamin D deficient diet

TD.88163 Vitamin E deficient diet

TD.95247 Folic acid deficient diet

Most vitamin deficient diets use vitamin-free test casein (VFT casein) as the protein source. VFT casein is casein that has been extracted with denatured alcohol to reduce the fat content from about 1% to 0.1%. In the process the content of a number of vitamins (present at low or trace levels in casein) is further reduced, making this an ideal protein source for these types of diets. Envigo produces our own VFT in-house, and we also offer this as an ingredient for those mixing their own diets.

From this vitamin deficient base, various levels of vitamins can be added back. Many formulas adjust multiple vitamins, and other nutrients.

If use of an intact protein source is not advisable, an amino acid defined diet can be used.

一些维生素调整公式如下所示。请与我们联系,以获得更多的这种性质的配方或更多的信息,以改变维生素的饮食概况,并请阅读下面来了解更多。

公式示例:

TD.86143维生素A缺乏饮食

TD.89123维生素D缺乏饮食

TD.88163维生素E缺乏饮食

TD.95247叶酸缺乏饮食

大多数缺乏维生素的饮食使用无维生素的测试酪蛋白(VFT酪蛋白)作为蛋白质来源。VFT酪蛋白是用变性醇提取的酪蛋白,使脂肪含量从1%左右降至0.1%左右。在这个过程中,一些维生素的含量(在低水平或微量的酪蛋白)被进一步减少,使之成为这些类型的饮食的理想的蛋白质来源。Envigo生产我们自己的VFT内部产品,我们也为那些混合他们自己的饮食的人提供这种材料。

从这个缺乏维生素的基础,不同水平的维生素可以补充回来。许多配方调整多种维生素和其他营养。

如果使用完整的蛋白质源是不可取的,氨基酸限定饮食可以使用。

上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫诊断试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

上海金畔生物科技有限公司

服务热线:18301939375   QQ:3258089810    3259632176

Email:  info@jinpanbio.com

官网:http://www.jinpanbio.com