Harlan Teklad LM-485 Mouse/Rat Sterilizable Diet

Harlan Teklad LM-485 Mouse/Rat Sterilizable Diet

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Home +Teklad diet, bedding and enrichment +Teklad laboratory animal diets +Standard natural ingredient diets +Rodent diets +Traditional rodent diets

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer Teklad Global Rodent Diet® line for your modern research needs.

传统的啮齿动物饮食是几十年前根据对啮齿动物营养,成分和当时饮食制造的了解而制定的。 尽管传统饮食可以满足实验动物的已知营养需求,但我们建议您考虑使用我们较新的Teklad Global RodentDiet®系列饮食来满足您的现代研究需求。

Products

TEKLAD TRADITIONAL RODENT DIETS NON-AUTOCLAVABLE FORM AUTOCLAVABLE IRRADIATED
Teklad 4% fat mouse/rat diet 7001 
Teklad S-2335 mouse breeder sterilizable diet 7004  7904 
Teklad LM-485 mouse/rat sterilizable diet 7012  7912 
NIH-31 Modified Open Formula Mouse/Rat sterilizable diet 7013  7913 
NIH-31 Open formula mouse/rat sterilizable diet 7017  7917 
NIH-07 Mouse/rat diet 7022  7922 
Teklad low salt mouse/rat sterilizable diet 7034 
Teklad rodent diet 8904 , 8604 , 8728C 
Teklad 22/5 rodent diet 8640 
Teklad sterilizable rodent diet 8656  7956 

Harlan Teklad 2018 global 18% protein rodent diets

Harlan Teklad 2018  global 18% protein rodent diets

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

2018年全球18%蛋白质啮齿类饲料

Home + Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Standard natural ingredient diets + Rodent diets + 2018 Teklad global 18% protein rodent diets

Product Features

  • Designed to support gestation, lactation, and growth
  • Formulated to reduce soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from 150 to 250 mg/kg
  • Exclusion of alfalfa reduces chlorophyll, greatly improving fluorescent optical imagingclarity
  • Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)
Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 18% protein 2018, 2018C 2018S, 2018SX 2918
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.

产品特点

  • 用于支持妊娠、哺乳和生长的
  • 减少豆粕的配方,从而尽量减少异黄酮的存在。异黄酮是大豆的主要类型。植物雌激素在实验室动物饮食中发现的
  • 典型的异黄酮浓度(大豆苷元+染料木素苷元当量)为150至250毫克/千克。
  • 紫花苜蓿的排斥降低了叶绿素含量,大大提高了叶绿素含量。荧光光学成像清晰性
  • 缺乏动物蛋白质和鱼粉可使亚硝胺(一种潜在的致癌物质)的存在减少到最低限度。
产品
特克勒德啮齿类食物 不可蒸压式 高压釜 辐照
Teklead全局18%蛋白 2018,2008 c 2008 S, 2018SX 2918
并不是所有的产品都是在当地库存的,可能需要延长交货期和额外的费用。
许多饮食都是由符合产品代码的“C”指定的认证格式提供的。当饮食被认证时,一个有代表性的样本会被测试一组污染物。如果没有经过认证的库存,可以根据要求提供认证。可适用最低订货量和附加费用。

Harlan Teklad 诱导动物NAFLD and NASH饲料

Harlan Teklad 诱导动物NAFLD and NASH饲料

Teklad diet, bedding and enrichment    Teklad laboratory animal diets   Custom research diets   NAFLD and NASH

诱导啮齿动物NAFLD/NASH的饮食方法可分为两大类:

  • 长期喂饲可诱发肥胖、代谢综合症及轻微纳什或
  • 短时间喂饲可诱发严重纳什的肝脏特征而不引起肥胖或胰岛素抵抗的饮食。

下表突出了上述两个类别的饮食选择。有关NAFLD/NASH模型的更完整描述,请参见表后面的下拉菜单。

Dietary methods to induce NAFLD/NASH in rodents can be split into two common categories:

  • diets fed for longer periods of time to induce obesity, metabolic syndrome, and mild NASH or
  • diets fed for short periods of time to induce hepatic features of severe NASH without inducing obesity or insulin resistance

This page provides further information on dietary methods to induce NAFLD/NASH. We’ve also prepared a downloadable NASH/NAFLD mini paper.

The tables below highlight diet options from both of the above categories. For more complete descriptions of NAFLD/NASH models see the drop down menus that follow the tables.

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH
Diet features Western/Fast Food ALIOS FPC diet
Product Code TD.88137 TD.06303 TD.160785 PWD dough

TD.190142 pellet

Fat, % Kcal 42 45 52
Fat Sources,
% by weight
21% milk fat 22% hydrogenated vegetable oil
1% soybean oil
19% hydrogenated vegetable oil
6% milk fat
4% palmitic acid
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
23% saturated
31% monounsaturated (cis)
12% polyunsaturated (cis)
34% trans
43% saturated
27% monounsaturated (cis)
7% polyunsaturated (cis)
23% trans
Sugars, % by weight 34.5% sucrose 22.4% sucrose 34.5% sucrose
Cholesterol, % by weight 0.2 0 1.25
Modifications TD.96121 1.25% cholesterol
TD.120528 Increased sucrose, 1.25% cholesterol
TD.120330 0.2% cholesterol
TD.130885 0.2% cholesterol, 27% sucrose
TD.140154 adds customer supplied palmitic acid

For high fat diet options to induce uncomplicated NAFLD see our Diet Induced Obesity page.

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome
Diet features High Fat, Cholesterol & Cholate Methionine/choline deficient (MCD)
Product Code TD.02028 TD.90262
Fat, % Kcal 42 22
Fat Sources,
% by weight
21% milk fat 10% corn oil
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
14% saturated
28% monounsaturated
58% polyunsaturated
Sugars, % by weight 33.3% sucrose 46% sucrose
Cholesterol, % by weight 1.25 0
Cholate Source, % by weight 0.5 0
Related diets TD.09237 15% milk fat, 1% cholesterol
TD.88051 Hybrid version
TD.94149 MCD control diet

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western or fast food style diets fed to induce NASH with metabolic syndrome contain 40 – 45% kcal from milkfat (a fat source high in palmitate) with added cholesterol (0.15 – 2%) and are high in sucrose (>30%). Dietary palmitate and cholesterol have both previously been associated with the progression from simple steatosis to NASH.

Examples:

  • TD.88137       Adjusted Calories Diet (42% from fat)
  • TD.96121       21% MF, 1.25% Chol. Diet
  • TD.120528     42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)

Research use:

These diets can induce obesity, metabolic syndrome, and simple steatosis within nine weeks of feeding. Increased hepatic inflammation has been observed after 12 weeks of feeding. NASH typically requires longer feeding with fibrosis developing within nine months and late stage fibrosis including hepatic ballooning occurring after 14 – 20 months of feeding. Increasing dietary sucrose (~41%) and cholesterol (~1.25%) accelerates the NASH phenotype with steatosis, inflammation and hepatocyte ballooning observed within 12 weeks. In addition to feeding a high fat diet, providing a glucose/fructose mixture in the drinking water may further promote NASH development.

Select References:

Charlton, M., et al., Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol, 2011. 301(5): p. G825-34. http://www.ncbi.nlm.nih.gov/pubmed/21836057

Gores, G., Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H. Am J Physiol Gastrointest Liver Physiol, 2015. 308: p. G159. http://ajpgi.physiology.org/content/308/2/G159

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Ioannou, G.N., et al., Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis. J Lipid Res, 2009. 54(5): p. 1326-34. http://www.ncbi.nlm.nih.gov/pubmed/23417738

Alkhouri, N., et al., Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis. J Biol Chem, 2010. 285(5): p. 3428-38. http://www.ncbi.nlm.nih.gov/pubmed/19940134

Dixon, L.J., et al., Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One, 2013. 8(2): p. e56100. http://www.ncbi.nlm.nih.gov/pubmed/23409132

DeLeve, L.D., et al., Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis. Am J Pathol, 2008. 173(4): p. 993-1001. http://www.ncbi.nlm.nih.gov/pubmed/18772330

VanSaun, M.N., et al., High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol, 2009. 175(1): p. 355-64. http://www.ncbi.nlm.nih.gov/pubmed/19541928

Asgharpour, A., et al., A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol, 2016. 65(3): p. 579-88. http://www.ncbi.nlm.nih.gov/pubmed/27261415

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Tsuchida, T., et al., A simple diet-and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer. Journal of hepatology, 2018. 69(2):385-395. https://www.ncbi.nlm.nih.gov/pubmed/29572095

The American Lifestyle-Induced Obesity Syndrome (ALIOS) model involves feeding the “American fast food” diet high in trans-fats and sugar. Dietary trans-fats from hydrogenated vegetable shortening (HVO) are associated with increased insulin resistance and hepatic inflammation in rodent NASH models. In addition to diet, a glucose/fructose solution is added to the drinking water and sedentary behavior promoted by removing the overhead cage feeders in this model.

Examples:

  • TD.06303       22% HVO Diet
  • TD.120330     22% HVO + 0.2% Cholesterol Diet
  • TD.130885     ALIOS with Added Sugar

Research use:

The ALIOS model develops obesity with insulin resistance, elevated ALT levels, and steatosis within 16 weeks. Increased inflammation and early development of fibrosis have been observed at 6 months. Severe steatosis with fibrosis and inflammation develops within 12 months of feeding with 50% of the mice reportedly developing hepatic neoplasms. Adding cholesterol (0.2%) to the American Fast Food diet may accelerate NASH phenotype development.

Select References:

Koppe, S.W., et al., Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol, 2009. 297(2): p. G378-84. http://www.ncbi.nlm.nih.gov/pubmed/19541924

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Mells, J.E., et al., Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol, 2012. 302(2): p. G225-35. http://www.ncbi.nlm.nih.gov/pubmed/22038829

Dowman, J.K, et al., Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle. Am J Pathol, 2014. 184(5):1550-1561. https://www.ncbi.nlm.nih.gov/pubmed/24650559 

Mells, J.E., et al., Saturated fat and cholesterol are critical to inducing murine metabolic syndrome with robust nonalcoholic steatohepatitis. J Nutr Biochem, 2014. 26(3): p. 285-92. http://www.ncbi.nlm.nih.gov/pubmed/25577467

The Fructose, Palmitate, Cholesterol and Trans-Fat (FPC) diet is a recent NASH diet that includes Western and ALIOS model diets to achieve both metabolic and hepatic NASH features within an accelerated time frame. Key features of the FPC diet include 1) a lower Met content than typical rodent diets by decreasing total protein without supplementing sulfur amino acids; 2) choline supplementation is lower than typical but is not considered deficient; 3) high in sucrose (~34% by weight); 4) 1.25% cholesterol; 5) 52% kcal from fat with fat sources including milkfat fat, palmitic acid and hydrogenated vegetable shortening to provide trans-fats. Like the ALIOS model, the FPC model also provides a glucose/fructose solution to the drinking water.

Examples:

  • TD.160785     52 kcal/Fat Diet (C16:0, HVO, AMF, Choline/Met)

Research use:

Male C57BL/6J mice fed the FPC diet and provided a glucose/fructose drinking solution developed insulin resistance and NAFLD with inflammation, hepatocyte death, and fibrosis within 16 weeks.

Select References:

Wang, X., et al., Hepatocyte TAZ/WWTR1 promotes inflammation and fibrosis in nonalcoholic steatohepatitis. Cell Metab, 2016. 24(6): p. 848-62. https://www.ncbi.nlm.nih.gov/pubmed/28068223  

Zhu, C., et al., Hepatocyte Notch activation induces liver fibrosis in nonalcoholic steatohepatitis. Sci Transl Med, 2018. 10(468). https://www.ncbi.nlm.nih.gov/pubmed/30463916

Common diets to induce obesity (DIO) can be fed to induce uncomplicated NAFLD. These high fat diets typically contain 40–60% kcal from fat without supplemented cholesterol or cholate. Simple sugars such as sucrose or fructose can also be supplemented via diet or water to progress the fatty liver phenotype. Diets can be in pellet or powder/dough form depending on the formula. Some models require limited physical activity and in those cases diets can be fed inside the cage. For more information see our Diet Induced Obesity page.

Examples:

  • TD.08811       45%kcal Fat Diet (21% MF, 2% SBO)
  • TD.06414       Adjusted Calories Diet (60/Fat)

Research use:

In susceptible rodent models, high fat diets are commonly used to induce NAFLD with obesity and insulin resistance common metabolic features associated with NASH in humans. However, the degree of NASH pathology (steatosis, inflammation, and fibrosis) is limited or mild and varies depending on the animal model, length of feeding, and dietary components.

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic

COLLAPSE ALL TABS

Originally formulated to induce mild atherosclerosis in wild-type rodents, high fat diets containing added cholesterol (1 – 1.25%) and cholate (0.5% as sodium cholate or cholic acid) have also been useful in inducing NASH. This diet option includes purified “Western” style diets with increased cholesterol and cholate and also hybrid diets. Hybrid diets were originally developed by Beverly Paigen and colleagues by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate) resulting in a diet containing ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Although a less refined approach, the hybrid diet is associated with increased gallstone formation and liver damage as compared to similar purified diets.

Examples:

  • TD.02028       Atherogenic Rodent Diet
  • TD.88051       Cocoa Butter Diet and Purina Mouse Chow
  • TD.09237       15% AMF Diet (1% Chol, 0.5% NaChol)

Research use:

Atherogenic diets are able to induce varied degrees of NASH with increased hepatic inflammation with early fibrosis observed after ten weeks of feeding. However, the metabolic profile typical in human NASH (obesity with insulin resistance) is not recapitulated in this model with animals typically maintaining similar body weights as control fed groups without the development of metabolic syndrome.

Select References:

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69. http://www.ncbi.nlm.nih.gov/pubmed/2380634

Kamari, Y., et al., Lack of interleukin-1alpha or interleukin-1beta inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice. J Hepatol, 2011. 55(5): p. 1086-94. http://www.ncbi.nlm.nih.gov/pubmed/21354232

Kim, D.G., et al., Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model. J Neuroinflammation, 2016. 13: p. 1. http://www.ncbi.nlm.nih.gov/pubmed/26728181

Madrigal-Perez, V.M., et al., Preclinical analysis of nonsteroidal anti-inflammatory drug usefulness for the simultaneous prevention of steatohepatitis, atherosclerosis and hyperlipidemia. Int J Clin Exp Med, 2015. 8(12): p. 22477-83. http://www.ncbi.nlm.nih.gov/pubmed/26885230

Savransky, V., et al., Chronic intermittent hypoxia causes hepatitis in a mouse model of diet-induced fatty liver. Am J Physiol Gastrointest Liver Physiol, 2007. 293(4): p. G871-7. http://www.ncbi.nlm.nih.gov/pubmed/17690174

Methionine and choline deficient (MCD) diets are amino acid defined rodent diets deficient in methionine and choline, high in sucrose (>40% by weight) with ~10% corn oil by weight. Methionine and choline deficiency decreases fat oxidation and export of fat from the liver. Dietary sucrose is necessary for hepatic lipid accumulation and oxidation. The polyunsaturated fat in corn oil promotes hepatic lipid oxidation.

Example:

  • TD.90262       Methionine/Choline Deficient Diet

Control:

  • TD.94149       Amino Acid Control Diet

Research use:

Steatosis, increased serum alanine aminotransferase (ALT), inflammation, and hepatic fat oxidation has been observed within three weeks of feeding the MCD diet with fibrosis development after six weeks. This dietary model does not produce metabolic syndrome (an aspect of NASH in human models) and progressive weight loss (up to 40%) is associated with the MCD diet feeding.

蛋氨酸/胆碱缺乏症(MCD)日粮
蛋氨酸和胆碱缺乏(MCD)饲料是一种氨基酸定义的啮齿动物饲料,缺乏蛋氨酸和胆碱,蔗糖含量高(体重>40%),玉米油含量约10%。蛋氨酸和胆碱缺乏减少脂肪氧化和从肝脏输出脂肪。日粮蔗糖对肝脏脂质的积累和氧化是必需的。玉米油中的多不饱和脂肪促进肝脏脂质氧化。

例子:TD.90262 蛋氨酸/胆碱缺乏症饮食
管制:TD.94149 氨基酸控制饮食

研究用途:

观察到脂肪变性、血清丙氨酸转氨酶(ALT)升高、炎症和肝脂肪氧化反应。这种饮食模式不会产生代谢综合症(在人类模型中是NASH的一个方面)和累进性减肥(高达40%)与MCD饮食喂养有关。

Select References:

Pickens, M.K., et al., Dietary sucrose is essential to the development of liver injury in the MCD model of steatohepatitis. J Lipid Res, 2009. 50(10):2072-82.  http://www.ncbi.nlm.nih.gov/pubmed/19295183

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Lee, G.S., et al., Polyunsaturated fat in the methionine-choline-deficient diet influences hepatic inflammation but not hepatocellular injury. J Lipid Res, 2007. 48(8): p. 1885-96. http://www.ncbi.nlm.nih.gov/pubmed/17526933

Vetelainen, R., A. van Vliet, and T.M. van Gulik, Essential pathogenic and metabolic differences in steatosis induced by choline or methione-choline deficient diets in a rat model. J Gastroenterol Hepatol, 2007. 22(9): p. 1526-33. http://www.ncbi.nlm.nih.gov/pubmed/17716355

Leclercq, I.A., et al., Intrahepatic insulin resistance in a murine model of steatohepatitis: effect of PPARgamma agonist pioglitazone. Lab Invest, 2007. 87(1): p. 56-65. http://www.ncbi.nlm.nih.gov/pubmed/17075577

Kashireddy, P.R. and M.S. Rao, Sex differences in choline-deficient diet-induced steatohepatitis in mice. Exp Biol Med (Maywood), 2004. 229(2): p. 158-62. http://www.ncbi.nlm.nih.gov/pubmed/14734794

Dixon, L.J., et al., Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest, 2012. 92(5): p. 713-23. http://www.ncbi.nlm.nih.gov/pubmed/22411067

Dietary models of NAFLD/NASH continue to evolve with the goal of more accurately recapitulating both the metabolic and hepatic symptoms of human disease. Commonly researchers are studying the synergistic effects of various NASH dietary features to accelerate progression of the model and severity of liver disease.

A Teklad nutritionist can work with you to formulate new diets in order to investigate novel dietary models of NAFLD/NASH.

The choice of control diet is dependent on the specific research goal. Many researchers choose to compare their NAFLD/NASH diet-fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diet or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens).

Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component. In some cases, such as those studies feeding amino acid defined diets like the MCD model, a matched control diet is recommended given the very different formulations and protein sources of grain-based diets.

When making inferences about specific nutrients within the diet an ingredient matched, low fat control diet may be necessary. There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined and digestible) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible).

A very basic purified control diet would be AIN-93M TD.94048 or AIN-93G TD.94045. AIN-93 diets have a moderate amount of sucrose at ~10% with fat from soybean oil providing a healthy fatty acid profile.

Contact a nutritionist for an additional information and control diet recommendations.

对照日粮
控制饮食的选择取决于具体的研究目标。许多研究人员选择比较他们的NAFLD/纳什饮食喂养的动物和喂养一种天然成分的动物,谷物为基础的饮食(也称为标准饮食或周食)。这些饮食在营养来源和水平以及非营养因素(如植酸盐或植物雌激素)存在的情况下存在差异。

根据您的主要比较,它可能适合作为您的对照/参考组谷物基础的饮食。然而,这样的比较限制了对饮食模式和特定饮食成分的推断。在某些情况下,如那些研究喂食氨基酸定义的饮食,如mcd模型,建议一个匹配的对照饮食,考虑到非常不同的配方和蛋白质来源的谷物为基础的饮食。

当对饮食中的特定营养成分做出相应的推断时,低脂控制饮食可能是必要的。除了不同类型的碳水化合物外,还有许多不同水平和类型的脂肪,从蔗糖(高精制和可消化)到玉米淀粉(精制,但更复杂),再到抗性淀粉(精制,但不能完全消化)。

一种非常基本的纯正控制饮食将是-9300万。TD.94048或者是93g TD.94045。AIN-93日粮中含有适量的蔗糖~10%,大豆油中的脂肪提供了健康的脂肪酸谱。

Need more information? A Teklad nutritionist will work with you to determine if existing diets will meet your needs or formulate new diets to help you investigate novel dietary models of NAFLD/NASH. Contact us for a diet consultation.

Harlan teklad饲料常见问题以及使用方法

更完整的名字是标准天然成分饮食。这些饮食大量生产(至少2吨),并储存在一个或多个恩维戈特克勒德配送中心。标准的天然成分饮食是现成的,一般可以在下订单后几天内交付。这些饮食主要含有谷物(玉米、小麦)、豆类(豆粕),以及较小程度上的食材,如鱼粉、肉和骨粉。标准的天然成分饮食包括全球和传统的饮食,并可获得辐照和/或认证。

Envigo公司采用美国和欧洲按照同样严格的规格购买的标准化固定配方和配料,其独特的Teclad全球饮食方案为国家和跨国公司提供营养一致性,供其在全球范围内进行研究,最大限度地实现全球研究的一致性。全球饮食在所有国家都有相同的产品名称。全球啮齿动物的饮食中蛋白质含量低于大多数传统饮食,并且配方中不含紫花苜蓿,不含豆粕或少量豆粕,从而减少了天然食物的存在。植物雌激素.

Envigo为普通实验动物提供了各种传统的特克乐饮食。这些固定的配方奶饮食是由高质量的配料制成的。虽然传统的饮食将提供已知的实验室动物的营养需求,但我们建议研究人员考虑从我们的全球饮食系列中使用一种饮食。传统的啮齿动物饮食是在几十年前根据对啮齿动物的营养、成分和当时的饮食制造的理解而制定的。对植物雌激素等非营养物质对实验结果的影响认识有限。紫花苜蓿粉和豆粕是实验室动物日粮中植物雌激素的主要来源。这些成分,特别是豆粕,是在大多数传统的啮齿动物饮食中发现的。

固定配方饮食是根据一种从一种生产到下一种生产保持不变的配方生产的饮食。结合严格的原材料质量标准,这一方法确保了质量和一致性,方法是最大限度地减少营养变化和饮食中其他植物化学物质的变异性,这可能会影响一项研究。

认证饮食这些饮食已经被测试为标准的环境污染物面板,已知这些污染物能够干扰一项研究。这些饮食有助于确保一项研究符合FDA的良好实验室实践条例,要求定期进行饲料分析。由营养师签署的Envigo分析证书提供给客户。

我们的首要目标是为客户提供优质和一致的产品。这首先要确保获得的原材料只有最高的一致性和质量。原材料只能从同意符合我们质量标准的认可供应商那里获得。供应商定期接受审核,他们的产品在进入我们的工厂之前经过质量测试。每种产品都有固定的配方和既定的制造程序,以确保产品与每一种产品的生产一致。在产品分发给客户之前,完成过程中和最终的产品测试.Envigo利用公司在美国和加拿大拥有和运营的分销中心网络,而不是使用外部分销商。

没有一个确定的点,人们能够预测什么时候一个特定的饮食会变质或变得缺乏一个或多个营养。6个月保质期的共同指导方针是以北美长期的实践为基础的。在欧洲和亚洲,当地做法和监管监督方面的差异导致特克拉德标准天然成分饮食被例行使用到9个月,有时甚至在生产后12个月。这种实践经验,随着时间的推移,以及文献支持和维生素测试,使我们相信这些饮食继续支持动物的健康和研究的完整性,至少9个月后生产。如果你不确定当地的政策,请向你的机构寻求指导。

建议的储存条件:

  • 凉爽和干燥;在华氏70度或以下,湿度低于50%的理想值,但高达65%是可以接受的。
  • 清洁无害虫
  • 在原始包装中或容器中,防止连续暴露于光线下,并将空气暴露在最小范围内。

Envigo提供辐照饮食,床上用品和浓缩物品。辐照通过使产品暴露于钴-60的伽马电离辐射,减少产品所含生物的数量。受伽马射线照射的生物体在分子水平上受到破坏,往往具有致命的影响。最终的结果是产品中可存活微生物数量的减少。Envigo以最低剂量2.0 mrad(20 KGy)和最大5.0mrad(50 KGy)照射所有Teclad产品。

高压灭菌会破坏饮食中补充的维生素和氨基酸。为了弥补这种破坏,蒸压饮食含有补充维生素和补充氨基酸。蒸压式饮食被包装在穿孔袋中,当饮食被压压在袋内时,允许蒸压器的蒸汽渗透到整个饮食中。

植物雌激素是植物与内源性雌激素相互作用的天然产物。与内源性雌激素相比,它们在分子结构上有一定的相似性,并在一定程度上与雌激素受体结合。植物雌激素已被证明影响哺乳动物生理,包括癌症生长、动脉粥样硬化、钙磷代谢和行为。。这是研究人员关注的问题,因为标准的天然成分饮食通常由含有大量植物雌激素的成分组成。豆粕是标准天然成分饮食中常见的一种成分,含有一种被称为异黄酮的植物雌激素。两种主要异黄酮为染料木素和大豆苷元。紫花苜蓿粉是标准天然成分饮食中常见的第二种成分。它含有植物雌激素联素。

“自由”一词意味着完全和完全不存在植物雌激素。这将排除任何由天然成分组成的饮食。唯一一种完全不含植物雌激素的饮食是不含大豆蛋白的纯化饮食。然而,Envigo提供了四种Teclad全球啮齿动物饮食,不包括已知含有大量植物雌激素的紫花苜蓿粉和豆粕等成分。2014、2016、2019和2020倍的饮食虽然不是完全“免费”,但最好用最小的植物雌激素饮食来形容。每季度对大豆异黄酮(大豆植物雌激素)的饮食进行测试时,通常会发现含量从不到可检测到的到百万分之20不等。从某种角度来看,大多数传统的啮齿动物饮食中含有200到500 ppm的异黄酮。植物雌激素最低限度饮食,如2014、2016、2019和2020 X,在植物雌激素引起关注的研究中通常是可以接受的。

定制研究饮食

COLLAPSE ALL TABS

定制研究饮食都是小批量生产的,一般没有库存。这些饮食通常是为特定类型的研究目标而制定的。这些饮食可以由精制成分(净化饮食)、天然成分(谷物和其他在标准饮食中发现的成分)组成,也可以利用标准饮食作为添加其他成分的基础。这些饮食类型中的任何一种都可以作为添加客户供应成分(试验物品、药品、食品提取物等)。我们的数据库中大约有20,000份食谱,所以你需要的配方可能已经被创建了。我们还利用这个数据库作为制定新饮食的资源。我们将很高兴为您提供一份完整的配方表,包括配料和包含率、宏营养元素值、关键功能以及计划和订购信息。

纯化的饮食使用精制成分,如酪蛋白、蔗糖、玉米淀粉和纤维素。这些人类食品级成分有相对简单的化学成分(主要是一种营养分类),这一特征对于为研究目的操纵个别营养物很重要。此外,大多数精制成分含有非常有限的可能具有生物活性的非营养成分。这与天然成分(玉米、小麦、豆粕等)形成鲜明对比。在标准饮食中使用,它们有相对复杂的化学成分以及各种可能与生理无关的植物化学物质。精制的原料是从可靠的人类级产品供应商那里获得的,我们每年都从同样的供应商那里获得原料。此外,主要的蛋白质成分被分析为一个特定的营养面板,以确保最小的变化。因此,纯化的饮食将是可重复的,可以很容易地修改。常见的净化饮食的例子包括AIN-76A(CA.170481)、AIN-93G(TD.94045)和AIN-93m(TD.94048).

美国营养学会(ASN)(前美国营养研究所,AIN)提倡为啮齿类动物使用营养充足的净化饮食,因为许多研究人员可能不知道潜在的营养变量。“an-76公式”于1977年出版,并成为AIN-76A1980年进行了轻微的修订(增加了维生素K)。这种饮食已经得到了广泛的应用,并提出了一些改进的建议。这导致1993年出版了两个公式,阿恩-93g(用于生长、繁殖和哺乳)和阿恩-9300万(供维修)。条款J.Nutr。123:1939-1951(1993)讨论了若干修订的理由。其中一些变化包括大豆油而不是玉米油、添加其他碳水化合物来源和降低蔗糖、降低磷、取代蛋氨酸的胱氨酸、包含几种微量元素和一些维生素调节。维持饮食含有较少的蛋白质和脂肪。

每个公式都有一个唯一的标识号,并且在我们文件中的数千个公式中保留了这个身份。这确保了即使几年后也会使用同样的公式。在生产时,分配一个六位或七位数的“Rx”批号,允许在饮食生产的所有阶段进行可追溯性。这些详细的生产记录是为每一个生产项目。质控样品保存9个月.对每批主要蛋白质成分进行分析,以确定每批营养物的均匀性。对于这些精制的原料,要谨慎地保留信誉良好的供应商。

是的,我们可以帮助你在饮食中添加一种测试化合物或成分。这通常是一种方便的方法来给研究动物投药。在饮食中添加一种化合物时,有一些重要的安全和稳定性考虑因素。请看客户供应的配料更详细的信息。

一个电话或电子邮件交换与我们的一名工作人员将增加您收集的信息,从网站或发表的文献,你更有可能得到的饮食,最适合你的特殊需要。我们会小心保密,如有需要,会签署正式协议。

建议我们为什么需要饮食,以及什么动物种类。尽可能详细地讨论营养物质或感兴趣的成分。请避免主观描述,如“低,正常,高”,因为这些术语有不同的含义对不同的研究人员。向我们提供任何现有公式的副本,以复制或修改,以及任何期刊文章,您的研究可能的基础。请告诉我们是否必须对饮食进行辐照。

我们定制研究饮食的最低订单是3公斤。这是特别方便,当喂食少量的老鼠,特别是饮食,其中包含你的测试化合物。维生素和矿物质混合物的最低含量为500克。

价格取决于配方的细节,成分组成,订单数量和形式(颗粒或粉末)。如果您知道产品代码,请联系我们。

标准包装包括一个小盒子(9×9×11英寸),最多可装5公斤;中型箱(10×10×15英寸),最多可装10公斤;更大的重型箱(13×13×20英寸),内嵌手柄最多可容纳20公斤。每个容器还包括一个三百万聚内衬,以保持您的研究饮食尽可能新鲜。真空包装和特定数量的包装也有额外的成本。真空包装通常会减少装在盒子里的饮食量。

饮食是定制的,以满足每一个订单。从订单到装运之间的典型的准备时间是十个工作日.这方面的例外是TD.88137,TD.06414,和TD.0130625公斤或更少的订单,通常在一周内就能买到。如果需要辐照(加10个工作日)。我们经常可以满足您的紧急生产需要,额外的费用。过境时间取决于所使用的运送方法的类型(固定地面、通宵等)。重量和交货方式影响运输成本,这通常是预付的,并添加到发票。

没有一个确定的点,人们能够预测什么时候一个特定的饮食会变质或变得缺乏一个或多个营养。定制的研究饮食是容易腐烂的,应该在4摄氏度(华氏40度)或更低的温度冷藏(见Fullerton等)。JNutr.112:567-573,1982年)。作为一般的指导方针,饮食可以以这种方式储存长达六个月之久。冻结饮食可能会减缓反应过程,但没有足够的信息推荐过去六个月的饮食作为一般指导方针。超过推荐使用日期的饮食的使用将受到您的机构的协议的约束。

定制的研究饮食在配方上有很大差异。饮食中可能有一些值得缩短保质期的特点。为了了解更多关于储存和使用建议,并帮助你如果你的饮食特点,可能需要更短的保质期,我们已经编制了一个指南。

屏障设施或某些动物模型需要无菌饮食。大多数自定义的研究饮食是经不起高压灭菌的,然而,我们可以安排合格的饮食被辐照。照射剂量范围为2~5次MRAD,即20~50 kGy.涉及象征性费用和额外时间。

营养学家可以帮助你确定一个配方是否适合辐照。辐照会导致选择性的维生素流失,我们的做法是增加某些维生素混合物的包含率,以帮助确保最终的饮食中含有足够的维生素。辐照饮食是双袋装的。真空包装有助于降低高脂饲料中辐照诱导的过氧化物生成。

有些配方奶是相当常见或通用的,使用它们的人可能不认为是机密的,特别是当作者提供产品编号时。如果没有产品编号,配方可能很难追踪,然后必须与营养师讨论。我们将审查每个公式的历史和保密。

给我们至少一个描述,最好是完整的公式,我们会给你一个我们的能力作出这个公式的评估。

造粒饮食是有限制的。脂肪的水平和类型、碳水化合物的组成以及脂肪和碳水化合物的相互作用将影响我们的饮食能力。

“无维生素”试验酪蛋白是从普通酪蛋白中提取的一种醇提酪蛋白,通常含有90%-91%的蛋白质(%Nx 6.38)、0.1%的脂肪和4-6%的水分。醇提物减少脂肪、脂溶性维生素和一些B族维生素。这种蛋白质来源最适合用于维生素缺乏饮食,当研究人员想最小化“背景脂肪”在节食中。我们已经准备了多年的这种特殊原料,并继续提供给许多实验室。我们将根据大量需求制定特殊价格。对于1,000公斤或以上的订单,建议提前通知。如果您需要这种成分,请考虑Envigo作为您的首选来源。

是。一些用于制作定制研究饮食的高质量成分也可以提供给那些自己准备饮食的人。看见饮食成分想了解更多信息。

当引用特克拉德饮食时,包括一个描述,和一个产品编号–标准的谷物饮食通常是四个数字,而定制饮食通常以“TD”开头,然后是五到六个数字。以下列例子作为指南:

2016年特克乐全球16%蛋白质啮齿动物饮食,美国威斯康星州麦迪逊,Envigo

美国威斯康星州麦迪逊州Envigo,以脂肪,主要是猪油,60%的千卡热量喂养高脂饮食TD.06414,导致肥胖。

美国威斯康星州麦迪逊市,Envigo,TD.89123,TD.89123。

每种饮食都有一个独特的产品编号,让其他人可以通过联系,快速、轻松地获取有关您在研究中使用的饮食的信息。

没有。根据NIH OACU关于在实验动物中使用非药物级化学品/化合物的指南(2008年),用于便利使用一种化合物的车辆与制剂中的活性化合物一样重要。饮食作为一种交通工具,无论是作为整体还是作为其部件的总和,都不符合本文件所定义的药品等级标准。

你可能是因为八号的一篇文章而被你的IACUC问到这个问题的。TH“实验室动物的护理和使用指南”(2011年),其中规定,在所有与动物有关的程序中,应酌情使用药剂级化学品和其他物质(第31页)。一般而言,偏离“指南”中的“必须”或“应”声明需要你的机构IACUC的理由和批准。

替代给药技术(灌胃,注射)可以在处理过程中带来压力,增加潜在伤害的风险。压力可以通过行为和生理上的改变来影响动物的健康以及科学的参数.通过饮食给药是一种既定的方法,用于向动物提供测试化合物,这是非侵入性的,减少压力和潜在的伤害处理。因此,使用非药物级化合物作为一种传递方法是合理的,因为它有利于动物福利和实验设计。

进一步读:

实验动物的护理和使用指南:第8版(2011年)。国家研究委员会更新“保护和使用实验动物指南”委员会。

在实验动物中使用非药物级化学品/化合物的指南。(2008年)。美国国立卫生研究院动物保护和使用办公室动物研究咨询委员会。

实验室惯例会引起动物压力。(2004年)。巴尔科姆,J.P.,Barnard,N.D.和Sandusky,C.考虑顶级实验室Anim Sci 43,42-51。

No. According to the NIH OACU Guidelines for Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals (2008), the vehicle used to facilitate administration of a compound is as important of a consideration as the active compound in the preparation. Diet as a vehicle, either whole or as the sum of its parts, would not meet the standard for pharmaceutical grade as defined by this document.

You may have been asked this question by your IACUC due to a passage in the 8thedition of the Guide for the Care and Use of Laboratory Animals (2011) stating that pharmaceutical-grade chemicals and other substances should be used, when available, for all animal-related procedures (page 31). In general, deviations from “Must” or “Should” statements within the Guide require justification and approval by your institutional IACUC.

Alternative dosing techniques (gavage, injection) can introduce stress from handling and increase the risk for potential injury. Stress can affect animal well-being as well as scientific parameters through modifications in behavior and physiology. Dosing via diet is an established method for delivering test compounds to animals that is non-invasive, reducing stress and potential injury from handling. Therefore, use of non-pharmaceutical grade compounds as a delivery method is justified, as it is advantageous for both animal welfare and experimental design.

Further reading:

Guide for the Care and Use of Laboratory Animals: 8th Edition (2011). National Research Council Committee for the Update of the Guide for the Care and Use of Laboratory Animals.

Guidelines for the Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals. (2008). Animal Research Advisory Committee, Office of Animal Care and Use, NIH.

Laboratory routines cause animal stress. (2004). Balcombe, J.P., Barnard, N.D., and Sandusky, C. Contemp Top Lab Anim Sci 43, 42-51.

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
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啮齿动物饲料(2018,625强力霉素)

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.375
Doxycycline Hyclate 0.625

Footnote   Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用说明

This diet was designed to deliver a daily dose of 2-3 mg of doxycycline based on consumption of 4-5 g/d by a mouse. Doxycycline hyclate contains approximately 87% doxycycline.

这种饮食被设计为以小鼠每天4-5 g / d的消耗量提供2-3 mg强力霉素的日剂量。 盐酸多西环素含有约87%的多西环素。

Selected Nutrient Information

  % by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Doxycycline
  • Gene Induction
  • 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Harlan AIN diet formulas

Harlan AIN diet formulas

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

These formulas are purified diets with commonly used refined ingredients. These basic formulas are often modified for a specific research purpose, such as altering the fat source, a vitamin or mineral level, or adding a compound. See the FAQ section to learn more about the history of these formulas, AIN-76A, AIN-93G, and AIN-93M.

  • CA.170481 AIN-76A purified diet
  • TD.94045 AIN-93G purified diet
  • TD.94048 AIN-93M purified diet

These formulas (as well as other purified diets) do not contain alfalfa and can be used to reduce background autofluorescence in certain imaging applications. Our Global Rodent Diets are also alfalfa-free and are suitable for imaging work. Please contact us for further information about these formulas or modifications.

Other related product codes:

  • TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased)
  • TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased)
  • TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased)
  • TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil.

Harlan AIN diet formulas

这些配方是含有常用精制成分的纯正饮食。这些基本配方通常是为特定的研究目的而修改的,例如改变脂肪来源、维生素或矿物质水平,或添加一种化合物。见常见问题了解更多关于这些公式的历史的章节,AIN-76A,AIN-93g,和AIN-93m。

  • CA.170481AIN-76A纯饲料
  • TD.94045AIN-93g纯饲料
  • TD.9404893米纯饲料

这些配方(以及其他纯化饲料)不含苜蓿,可用于减少背景自荧光在某些成像应用中。我们的全球啮齿动物饮食也是无苜蓿的,适合成像工作.有关这些公式或修改的进一步信息,请与我们联系。

其他相关产品代码:

  • TD.94096适合辐照的AIN-76A版本(维生素含量增加)
  • TD.97184适合辐照的an-93g版本(维生素含量增加)
  • TD.00102AIN-93m适合辐照的版本(维生素含量增加)
  • TD.95092用玉米油代替大豆油的n-93g改性。

Harlan Teklad Diet ingredients

Harlan Teklad Diet ingredients

Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Diet ingredients

You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.

Our ingredients

We offer several ingredients for individual sale so that you can develop your own blends.

“VFT” casein

  • “Vitamin-free” test casein (alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
  • The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

  • The common vitamin mixes shown below use sucrose or corn starch as a carrier
  • Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
  • Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
  • For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
  • Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
  • Minimum order is 500 g for both stock and custom vitamin mixes
  • Our available vitamin mixes are commonly used in diets, and are available from stock
  • Vitamin mixes are shipped within a few days of order

Our Formula examples

  • CA.40060 Teklad vitamin mix
  • CA.40077 AIN-76A vitamin mix
  • TD.94047 AIN-93 vitamin mix

Mineral mixes

  • The common mineral mixes shown below as examples use sucrose as a carrier
  • Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
  • Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
  • For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
  • Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
  • These mineral mixes are commonly used in diets, and are available from stock
  • Our mineral mixes are shipped within a few days of order

Formula examples:

  • CA.170915 AIN-76 mineral mix
  • TD.94046 AIN-93G mineral mix
  • TD.94049 AIN-93M mineral mix

Other mixes (may or may not be available from stock):

  • TD.83171 Vitamin mix without A, D, E, choline
  • TD.81062 Iron deficient mineral mix based on AIN-76A
  • TD.79055 Calcium and phosphorus deficient mineral mixed based on AIN-76
  • TD.98057 Calcium and phosphorus deficient mineral mixed based on AIN-93
  • CA.170760 Rogers-Harper mineral mix

你可能想要在你的研究地点或设施准备你自己的饮食,如果是这样的话,你取决于成分是最高的质量。Envigo可以为您提供许多相同的成分,用于生产我们先进的特克拉德定制研究饮食,以便您可以开发自己的配方。和往常一样,我们的营养专家在这里帮助您为您的配料和最终配方做出最佳决定。

我们的配料

我们提供几个配料供个人销售,以便您可以开发自己的混合。

“VFT”酪蛋白

  • “无维生素”测试酪蛋白(酒精提取)最适合于那些需要精确控制脂肪或维生素含量的纯正试验饮食配方。
  • VFT酪蛋白中许多维生素含量的降低使得它成为许多维生素研究的首选蛋白质来源。因此,“无维生素”的绰号是在许多年前给提取出来的酪蛋白起的。

维生素混合物

  • 以下常见的维生素混合物使用蔗糖或玉米淀粉作为载体。
  • 定制的维生素混合物可能使用纤维素,如果非营养载体是必要的。
  • 维生素混合物的制定考虑到了饮食中的包容率。多用或少用会损害你的实验动物的健康。
  • 对于那些自己准备饮食的人来说,维生素预混剂是向饮食中添加维生素的一种有效方法。
  • 维生素混合物应储存在4摄氏度或更低的接收。如果使用时间超过1个月,建议使用摄氏-20度。
  • 库存和定制维生素混合物的最低订购量为500克。
  • 我们可用的维生素混合物通常用于饮食,并可从库存中获得。
  • 维生素混合物在订购后几天内发货。

我们的公式示例

  • CA.40060 特克拉德维生素混合物
  • CA.40077 AIN-76A维生素混合物
  • TD.94047 AIN-93维生素混合物

矿物混合物

  • 下面所示的常见矿物混合物是以蔗糖为载体的。
  • 如果需要非营养的载体,定制的矿物混合物可以使用纤维素,也可以不使用载体。
  • 我们的矿物质混合物是根据饮食包容率来制定的。多用或少用会损害动物的健康。
  • 对于那些自己准备饮食的人来说,矿物预混料是向饮食中添加矿物质的有效方法。
  • 矿物混合物应存放在干燥、凉爽的环境中,并在收到后一年内使用。库存和自定义矿物混合物的最低订购量为500克。
  • 这些矿物混合物通常用于饮食,并可从库存中获得。
  • 我们的矿物混合物在订货后几天内装运。

公式示例:

  • CA.170915 AIN-76矿物混合物
  • TD.94046 AIN-93g矿物混合物
  • TD.94049 AIN-9300万矿物混合物

其他混合材料(可能从库存中获得,也可能无法从库存中获得):

  • TD.83171不含A,D,E,胆碱的维生素混合物
  • TD.81062基于AIN-76A的缺铁矿物配料
  • TD.79055 AIN-76混合钙磷缺乏矿物
  • TD.98057AIN-93混合钙磷缺乏矿物
  • CA.170760 罗杰斯-哈珀矿物混合物

Harlan Teklad 2016 global 16% protein rodent diets

Harlan Teklad 2016 global 16% protein rodent diets

Product Features

  • Designed to support growth and maintenance
  • Formulated to exclude soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from non-detectable to 20 mg/kg
  • Exclusion of alfalfa reduces chlorophyll, greatly improving fluorescent optical imagingclarity
  • Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)
Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 16% protein 2016, 2016C 2016S 2916
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.

Teklad Global 16% Protein Rodent Diet

Product Description- 2016 is a fixed formula, non-autoclavable diet
manufactured with high quality ingredients and designed to support growth
and maintenance. 2016 does not contain alfalfa or soybean meal, thus
minimizing the occurrence of natural phytoestrogens. Typical isoflavone
concentrations (daidzein + genistein aglycone equivalents) range from nondetectable
to 20 mg/kg. Exclusion of alfalfa reduces chlorophyll, improving
optical imaging clarity. Absence of animal protein and fish meal minimizes
the presence of nitrosamines. Also available certified (2016C) and
irradiated (2916). For autoclavable diet, refer to 2016S (Sterilizable).

Macronutrients

Crude Protein % 16.4
Fat (ether extract) a % 4.0
Carbohydrate (available) b % 48.5
Crude Fiber % 3.3
Neutral Detergent Fiber c % 15.2
Ash % 4.9
Energy Density d kcal/g (kJ/g) 3.0 (12.6)
Calories from Protein % 22
Calories from Fat % 12
Calories from Carbohydrate % 66
Minerals    
Calcium % 1.0
Phosphorus % 0.7
Non-Phytate Phosphorus % 0.4
Sodium % 0.2
Potassium % 0.6
Chloride % 0.4
Magnesium % 0.2
Zinc mg/kg 70
Manganese mg/kg 100
Copper mg/kg 15
Iodine mg/kg 6
Iron mg/kg 200
Selenium mg/kg 0.23
Amino Acids    
Aspartic Acid % 1.0
Glutamic Acid % 3.3
Alanine % 0.9
Glycine % 0.7
Threonine % 0.6
Proline % 1.5
Serine % 0.8
Leucine % 1.9
Isoleucine % 0.7
Valine % 0.8
Phenylalanine % 0.9
Tyrosine % 0.5
Methionine % 0.3
Cystine % 0.3
Lysine % 0.8
Histidine % 0.4
Arginine % 0.8
Tryptophan % 0.2

Teklad Diets are designed and manufactured for research purposes only.

Ingredients (in descending order of inclusion)- Ground wheat, ground corn, wheat middlings, corn gluten meal, calcium carbonate, dicalcium phosphate, soybean oil, brewers dried yeast, iodized salt, L-lysine, DL-methionine, choline chloride, magnesium oxide, vitamin E acetate, menadione sodium bisulfite complex (source of vitamin K activity), manganous oxide, ferrous sulfate, zinc oxide, niacin, calcium pantothenate, copper sulfate, pyridoxine hydrochloride, riboflavin, thiamin mononitrate, vitamin A acetate, calcium iodate, vitamin B12 supplement, folic acid, biotin, vitamin D3 supplement,

cobalt carbonate.

Vitamins

Vitamin A e, f IU/g 15.0
Vitamin D3 e, g IU/g 1.5
Vitamin E IU/kg 110
Vitamin K3 (menadione) mg/kg 50
Vitamin B1 (thiamin) mg/kg 17
Vitamin B2 (riboflavin) mg/kg 15
Niacin (nicotinic acid) mg/kg 75
Vitamin B6 (pyridoxine) mg/kg 18
Pantothenic Acid mg/kg 33
Vitamin B12 (cyanocobalamin) mg/kg 0.08
Biotin mg/kg 0.40
Folate mg/kg 4
Choline mg/kg 1030
Fatty Acids    
C16:0 Palmitic % 0.5
C18:0 Stearic % 0.1
C18:1ω9 Oleic % 0.7
C18:2ω6 Linoleic % 2.0
C18:3ω3 Linolenic % 0.1
Total Saturated % 0.6
Total Monounsaturated % 0.7
Total Polyunsaturated % 2.1
Other    
Cholesterol mg/kg

 

  • Ether extract is used to measure fat in pelleted diets, while an acid hydrolysis method is required to recover fat in extruded diets. Compared to ether extract, the fat value for acid hydrolysis will be approximately 1% point higher.
  • Carbohydrate (available) is calculated by subtracting neutral detergent fiber from total carbohydrates.
  • Neutral detergent fiber is an estimate of insoluble fiber, including cellulose, hemicellulose, and lignin. Crude fiber methodology underestimates total fiber.
  • Energy density is a calculated estimate of metabolizable energybased on the Atwater factors assigning 4 kcal/g to protein, 9 kcal/g to fat, and 4 kcal/g to available carbohydrate.
  • Indicates added amount but does not account for contribution from other ingredients.
  • 1 IU vitamin A = 0.3 µg retinol
  • 1 IU vitamin D = 25 ng cholecalciferol

For nutrients not listed, insufficient data is available to quantify.

Nutrient data represent the best information available, calculated from published values and direct analytical testing of raw materials and finished product. Nutrient values may vary due to the natural variations in the ingredients, analysis, and effects of processing.

Harlan 啮齿类饲料 Rodent diets

Harlan 啮齿类饲料 Rodent diets

As a researcher, you want to have as much control as possible over what goes into your study animals. At Envigo, we understand that. That is why we developed Teklad Global Rodent Diets®.

Teklad Global Rodent Diets® are a special integrated range of vegetarian laboratory rodent diets developed to be nutritionally complete for various life stages from breeding through long-term maintenance. Global rodent laboratory diets contain levels of protein, energy, vitamins and minerals that are more appropriate to the needs of modern biomedical research studies.

Furthermore, in global rodent diets particular attention has been placed on avoiding, as far as practical, ingredients that are reported to have adverse confounding effects on experimental results. This has resulted in a range of Teklad rodent laboratory diets that contain:

No fish meal

No meat meals or meat by-products

No alfalfa meal

No soybean meal or reduced levels

No animal fat

By excluding animal by-products, the presence of nitrosamines (a potential carcinogen) is avoided. Exclusion of alfalfa meal reduces chlorophyll, improving optical imaging clarity. Reduction or removal of soybean meal, together with elimination of alfalfa meal, minimizes levels of naturally-occurring phytoestrogens. Phytoestrogens interact with endogenous estrogens and potentially can affect studies in many research areas. Read more about this in these pieces:

Dietary phytoestrogens, a source of research variation
Ask a Nutritionist Series: Impact of phytoestrogens on research, Volume 1

Phytoestrogens limit translation of preclinical results to clinical outcomes
Ask a Nutritionist Series: Impact of phytoestrogens on research, Volume 2

Products
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 14% protein 2014, 2014C 2014S 2914
Teklad global 16% protein 2016, 2016C 2016S 2916
Teklad global 18% protein 2018, 2018C 2018S, 2018SX 2918
Teklad global 19% protein extruded 2019 2019S 2919
Teklad global soy protein-free extruded 2020X 2020SX 2920X
Traditional diets see more
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.
产品
Teklad啮齿类食物 不可蒸压式 高压釜 辐照
总蛋白14% 2014 2014C 2014S 2914
总蛋白16% 2016 2016C 2016S 2916
Teklead全局18%蛋白 2018 2018C 2018S,2018SX 2918
Teklad 19%蛋白挤出 2019 2019S 2919
Teklad 无大豆蛋白挤出 2020 X 2020SX 2920 X
传统饮食 见更多
并不是所有的产品都是在当地库存的,可能需要延长交货期和额外的费用。

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer global diet line for your modern research needs.

Teklad Global Rodent Diets® are modern formulas designed to reduce research variables. Specifically, these diets contain more appropriate nutrient levels, and limit or exclude ingredients that are reported to have effects on a wide variety of research endpoints.

  • Lower, more appropriate protein levels can improve survival and reduce morbidity
  • Vegetarian with no nitrosamines (a potential carcinogen)
  • Formulated to exclude alfalfa meal, greatly improving fluorescent imaging clarity
  • Formulated to exclude or lower soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Extruded rodent diets dramatically reduce clumping and hardness after autoclaving (2018SX, 2019S, 2020SX), and in general result in less diet waste and cleaner cages

Teklad rodent diets are natural-ingredient diets specifically formulated to provide the proper balance of all known nutrients considered essential for the growth, maintenance, and reproduction of rats, mice, gerbils and hamsters. These diets conform to the nutrient requirements for rodents established by the National Research Council (1995).

Teklad rodent diets provide uniform nutrition. They are fixed-formula diets designed to minimize the nutrient variances which otherwise could occur if the ingredient composition of a diet were altered from one batch to the next.

Protein is supplied primarily by plant sources. Supplemental amino acids are added to provide the proper amount and balance of essential amino acids. All rodent diets are fortified with vitamins and minerals to help support the regulation of body fluids and the proper functioning of body systems to ensure the adequate growth, maintenance, and reproduction of research rodents. Autoclavable diets are supplemented with additional vitamins to compensate for losses that occur during autoclaving. Since our diets are nutritionally complete and balanced, it is not necessary to add dietary supplements.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a six month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to nine months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least nine months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

Recommended storage conditions:

  • Cool and dry; at or below 70 degrees fahrenheit with humidity ideally below 50%, but up to 65% is acceptable
  • Clean and free of pests
  • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

Standard natural ingredient rodent diets are complete diets for rats, mice, gerbils and hamsters, that should be allowed ad libitum access to the diet. Fresh water should be available at all times.

Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

A staple of atherosclerosis research

More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model.

With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.

Formula g/Kg
Casein 195.0
DL-Methionine 3.0
Sucrose 341.46
Corn Starch 150.0
Andydrous Milkfat 210.0
Cholesterol 1.5
Cellulose 50.0
Mineral Mix, AIN-76 (170915) 35.0
Calcium Carbonate 4.0
Vitamin Mix, Teklad (40060) 10.0
Ethoxyquin 0.04

Selected nutrient information1

  % By weight % kcal from
     
Protein 17.3 15.2
Carbohydrate 48.5 42.7
Fat 21.2 42.0
     
kcal/g 4.5  
Cholesterol2 0.2%  
  • Values are calculated from ingredient analysis or manufacturer data.
  • 15% added, 0.05% from fat source.

Critical dietary features of TD.88137 related to atherosclerosis development include:

++Cholesterol (0.2% total cholesterol)

++Total fat (21% by weight; 42% kcal from fat)

++High in saturated fatty acids

(>60% of total fatty acids)

++High sucrose (34% by weight)

Typical fatty acid profile of TD.88137

Typical fatty acid analysis, Mean SD  
% of diet1  
     
Total 20.7 1.5  
Saturated fat 12.8 0.8  
Monounsaturated fat 5.6 0.5  
Polyunsaturated fat 1.0 0.2  
Unknown2 1.3 0.3  
       
Typical fatty acid profile, Mean SD  
% of total fatty acids1  
     
Saturated fat 61.8 2.0  
Monounsaturated fat 27.3 2.1  
Polyunsaturated fat 4.7 0.8  
4:0 2.1 1.1  
6:0 1.5 0.7  
8:0 1.1 0.3  
10:0 2.6 0.5  
12:0 3.3 0.5  
14:0 10.6 0.9  
16:0 28.9 1.3  
16:1 1.5 0.2  
18:0 12.5 0.8  
18:1 (Oleic) 20.9 2.6  
18:1 Isomers3 4.0 1.2  
18:2 (Linoleic) 2.3 1.0  
18:2 Isomers4 1.3 0.5  
18:3 (Linolenic) 0.7 0.2  
  • n = 21, analysis conducted by two independent laboratories.
  • Unidentified fatty acids and those contributing on average less than 0.5% of total fatty acids.
  • Includes trans isomers elaidic and vaccenic acid and unidentified cis isomers.
  • Includes trans isomers.

Key points from the literature

TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:

++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks (2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).

++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic arch (6, 7).

For further information about TD.88137, or if you are interested in learning more about other atherogenic or high fat diets contact us at

With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis, hypertension and metabolic syndrome. Contact us for a more extensive reference list.

Control diet options for TD.88137

Natural ingredient diets

++Also referred to as standard diets or chow

++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared to TD.88137

++Limits inferences to differences in dietary pattern versus a specific dietary component

Ingredient matched, low fat diets

++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients

++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at

Suggested ingredient matched, low fat controls

Diet kcal/g Fat, % by % kcal Fat sources, Sucrose, %
    weight from fat % by weight by weight
        3.7% milk fat,  
TD.05230 3.7 5.2 12.6 1.3% soybean 34.1
        oil  
           
        3.7% milk fat,  
TD.08485 3.6 5.2 13.0 1.3% soybean 12.0
        oil  
           

Additional controls are available. Contact a nutritionist at

Key planning information:

  • Store diet refrigerated and plan to use within six months. Diet should be replaced at minimum once per week when fed on cage tops.
  • Diets available as a soft ½” pellet or as a crumbly powder.
  • Three kg minimum order quantity. For planning purposes, estimates for diet uses (including feed intake and diet waste) are 5 g of diet per mouse and 30 g of diet per rat per day.
  • Two-day lead time for orders less than 10 kg. Two-week lead time for larger quantity orders.
  • Lead time for irradiation adds two weeks for any quantity of diet and must be requested at the time you place your order. Changes in texture and browning may occur with irradiation.
  • Shipping can affect pellet quality. Vacuum packaging can offer protection of the pellets during shipping. Two-day shipping is recommended during warmer months.

Contact us to place an order,

References

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  2. Nakashima, Y., et al.,Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994. 14(1): p. 133-40.
  3. Febbraio, M., et al.,Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.

J Clin Invest, 2000. 105(8): p. 1049-56.

  1. Nakashima, Y., et al.,Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb

Vasc Biol, 1998. 18(5): p. 842-51.

  1. Towler, D.A., et al.,Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.

J Biol Chem, 1998. 273(46): p. 30427-34.

  1. Tsuchiya, K., et al.,FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
  2. Huszar, D., et al.,Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
  3. Yang, B., et al.,Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
  4. Schafer, K., et al.,Leptin promotes vascular remodeling and neointimal growth in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
  5. Lijnen, H.R., et al.,Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc Biol, 2003. 23(1): p. 78-84.
  6. Maquoi, E., et al.,Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002. 51(4): p. 1093-101.
  7. VanSaun MN, et al. High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol 175:355-64.
  8. Dixon LJ, et al. Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One 8:e56100.