Harlan Teklad 他莫昔芬动物饲料 Tamoxifen diets

Harlan Teklad 他莫昔芬动物饲料 Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

Users are encouraged to select from the stocked options below supply readily available to ship within days.

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate
Tamoxifen
mg per kg diet
Daily tamoxifen
mg per kg body weight1
Diet code
(Teklad supplied tamoxifen)
    Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.1308603

1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125)

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

COLLAPSE ALL TABS

  • Minimum order quantity is three kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)
  • To place an order: contact Teklad Customer Service at 800.483.5523 or initiate order online

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet, one week off) for longer treatments
  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.
  • Length of treatment varies from one to two weeks1,2,5,6,7,8 to one to two months3,4,8
  • Pure tamoxifen1,5,8 and tamoxifen citrate1,2,3,4,6,7 are both effective
  • Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8 or 500 mg1,5 and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7
  1. Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.
  2. Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.
  3. Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.
  4. Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.
  5. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.
  6. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.
  7. Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.
  8. Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.

Harlan 强力霉素饲料 Doxycycline diets

Harlan 强力霉素饲料 Doxycycline diets

Harlan代理,Harlan饲料代理,欢迎访问Harlan官网或者咨询我们获取更多相关产品信息。

Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1 – 2 days (non-irradiated) or 1 – 2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don’t find a suitable option below.

EnvigoTeclad饮食使各种多西环素包含饮食为您的Tet调节系统。TD.01306以625毫克/千克的强力霉素添加到2018年全球啮齿动物饲料中,通常可在1至2天(未辐照)或1-2周(辐照)内装运。饮食很容易定制。定制规格包括:基础饮食,多西环素浓度和食用色素。联系我们如果你在下面找不到合适的选择的话,来讨论你的需求。

Formula examples with Teklad global 2018 base diet
Doxycycline hyclate1 USP (mg/kg diet) Natural color Alternatives with food coloring
mg per kg diet
46 TD.10483 TD.180327 Red
TD.120240 Blue
100 TD.04233 TD.130840 Blue
200 TD.00502 TD.180625 Red
TD.150431 Blue
TD.04104 Green
625 TD.01306
stocked2
TD.08541 Red
TD.120769 Blue
TD.09628 Green
1000 TD.05298 TD.06294 Red
TD.120658 Blue
2000 TD.05512 TD.140011 Red
TD.09633 Green
6000 TD.01533

1 Doxycycline hyclate is ~87% doxycycline
2 TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Considerations when choosing a base diet

    • Teklad Global Rodent Diets® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
    • Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.
Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate1 at 625 mg/kg diet
Base diet Natural color Alternatives with food coloring
mg per kg diet
2014 TD.00426 TD.07382 Red
TD.07573 Yellow
2016 TD.02503 TD.140263 Blue
TD.170364 Red
2019 TD.09651 TD.09761 Green
2020 TD.110720 TD.130141 Red

1 Doxycycline hyclate is ~87% doxycycline

Advantages of diet delivery of doxycycline

  • Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
  • Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
  • Diet is typically changed once per week. Water may need to be changed every few days
  • Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

Key planning information

  • Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
    • TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation
  • Irradiation (20 – 50 kGy) is optional, and must be requested at time of order

Safe handling of doxycycline diets

  • Doxycycline is a tetracycline class antibiotic.
  • Usual dose for therapeutic effects in humans is 100-200 mg/day. Doxycycline diets for rodents typically contain ~2 mg/pellet.
  • Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

多西环素饮食给药的优势

  • 典型的多西环素浓度可以用于饮食和水。低浓度的多西环素可能足够,625毫克/千克的饮食是最常见的浓度。
  • 饮食能保护身体免受光线伤害。水可能必须用深色或铝箔包装的瓶子浇水。
  • 饮食通常每周改变一次。水可能需要每隔几天更换一次。
  • 饮食供应也可以减少脱水的风险,并避免需要给予蔗糖(考索恩等)。艾尔。(2007年)

关键规划信息

  • 最小订货量为3公斤,足以喂养20只小鼠一个月。
  • 储存冷冻饮食,并计划在六个月内使用。
  • 典型的准备时间为两周(如果经过辐照,则为四周)
    • TD.01306通常在未经辐照的2天内或在辐照的2周内装运。
  • 辐射(20-50 kGy)是可选的,必须按顺序要求。

多西环素饮食的安全处理

  • 强力霉素是四环素类抗生素。
  • 人体治疗效果的一般剂量为每天100至200毫克。用于啮齿动物的多西环素饮食通常含有~2毫克/粒。
  • 当处理饮食时,使用典型的实验室预防措施,如实验室外套、手套和口罩,可以将多西环素意外暴露量降到最低。
  • 您的化学安全部门应联系,以获得额外的机构特定的指导方针,以处理和处置多西环素的饮食。

Harlan饲料问与答 Diet FAQs

Harlan饲料问与答 Diet FAQs

上海金畔生物代理Harlan饲料,欢迎访问Harlan官网或者咨询我们获取更多相关产品信息。

Standard Natural Ingredient Diets

A more complete name would be Standard Natural Ingredient Diets. These diets are manufactured in large quantities (at least 2 tons) and stocked at one or more Envigo Teklad distribution centers. Standard natural ingredient diets are readily available and can generally be delivered within a few days after an order is placed. These diets primarily contain grains (corn, wheat), legumes (soybean meal) and to a lesser extent ingredients such as fish meal, and meat and bone meal. Standard natural ingredient diets include Global and Traditional diets and are available Irradiated and/or Certified.

Using standardized fixed formulas and ingredients purchased under the same stringent specifications in the United States and Europe, Envigo’s unique Teklad Global Diets Program provides nutritional consistency to national and multinational companies for their worldwide research, maximizing global study uniformity. Global Diets have the same product names in all countries. The Global Rodent diets have lower protein levels than most traditional diets and are formulated free of alfalfa with zero or modest amounts of soybean meal, thus minimizing the presence of natural phytoestrogens.

Envigo offers a variety of Traditional Teklad diets for the common laboratory animal species. These fixed formula diets are made from high quality ingredients. While traditional diets will supply the known nutrient needs of laboratory animals, we recommend that researchers consider the use of a diet from our Global Diet line. The traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. There was limited appreciation of the effects of non-nutrients, such as phytoestrogens, on experimental results. Alfalfa meal and soybean meal are the major sources of phytoestrogens in laboratory animal diets. These ingredients, particularly soybean meal, are found in most traditional rodent diets.

Fixed Formula diets are diets that are manufactured in accordance with a formula that remains unchanged from one production to the next. In conjunction with strict quality standards for raw materials, this approach ensures quality and consistency by minimizing nutrient variability and the variability of other phytochemicals in the diet which might affect a research study.

Certified diets are diets that have been tested for a standard panel of environmental contaminants that are known to be capable of interfering with a study. These diets help ensure that a study complies with the FDA’s Good Laboratory Practices regulations requiring periodic feed analysis. An Envigo certificate of analysis signed by a nutritionist is made available to the customer.

Our #1 goal is to provide quality and consistent products to our customers. This begins with ensuring that raw materials of only the highest consistency and quality are obtained. Raw materials are only obtained from approved suppliers who agree to meet our quality standards. Suppliers are audited on a regular basis and their products are tested for quality before being accepted into our facilities. Fixed formulas and established manufacturing procedures are in place for each product which ensure that the product is manufactured consistently with each production. In-process and final product testing is completed prior to distribution of the product to a customer. Envigo utilizes its own network of company owned and operated distribution centers throughout the US and Canada rather than use outside distributors.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a 6 month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to 9 months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least 9 months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

没有一个人能够预测当特定的饮食会破坏或缺乏一个或更多的营养。在常见的 6 个月的货架寿命是基于长期的实践中。在欧洲和亚洲 , 不同地方的做法和监管标准 , 导致 Teklad 饮食天然成分通常使用了 9 个月和 12 个月后有时制造。这种实践的经验 , 再加上文学和测试支持 , 让我们继续支持这些膳食研究动物的健康和完整性至少 9 个月后制造。请参考您的机构的指导 , 如果你不确定的地方政策。

Recommended storage conditions:

  • Cool and dry; at or below 70 degrees Fahrenheit, humidity below 50% ideal, but up to 65% is acceptable
  • Clean and free of pests
  • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

建议贮藏条件 :

  • 冷却和干燥 ;在等于或低于 70 华氏度 , 湿度低于 50% , 但高达 65 % 是可接受的
  • 干净 , 没有害虫
  • 在原包装或容器 , 防止在连续的光暴露和最小暴露于空气中

Envigo offers irradiated diets, bedding, and enrichment items. Irradiation reduces the bioburden (number of organisms) contained by a product by exposing the product to gamma ionizing radiation from cobalt-60. Organisms exposed to gamma irradiation are damaged at the molecular level often with lethal effects. The net result is a decrease in the amount of viable microorganisms present in the product. Envigo irradiates all Teklad products at a minimum dose of 2.0 Mrad (20kGy) and a maximum of 5.0 Mrad (50 kGy).

Autoclaving destroys vitamins and amino acids that are supplemented in the diet. To compensate for this destruction, autoclavable diets contain an additional supplement of vitamins and supplemental amino acids. Autoclavable diets are packaged in perforated bags which allow steam from the autoclave to penetrate throughout the diet when the diet is autoclaved inside the bag.

Phytoestrogens are phytochemicals naturally produced by plants which interact with endogenous estrogens. They have some similarity in molecular structure compared to endogenous estrogens and bind to some extent with estrogen receptors. Phytoestrogens have been shown to affect mammalian physiology including cancer growth, atherosclerosis, calcium-phosphorus metabolism, and behavior. This is of concern to researchers because standard natural ingredient diets are often composed of ingredients which contain significant quantities of phytoestrogens. Soybean meal is an ingredient common to standard natural ingredient diets which contains a class of phytoestrogens called isoflavones. The two primary isoflavones are genistein and daidzein. Alfalfa meal is a second ingredient common to standard natural ingredient diets. It contains the phytoestrogen coumestrol.

Custom research diets

COLLAPSE ALL TABS

Custom research diets are made in small quantities and are generally not stocked. These diets are typically formulated for a specific type of research objective. These diets can consist of refined ingredients (purified diets), natural ingredients (grain and other ingredients found in standard diets), or utilize a standard diet as a base to which other ingredients are added. Any of these diet types can serve as the basis to add a customer supplied ingredient (test article, pharmaceutical, food extract, etc). We have approximately 20,000 diets in our database, so the formula you require may already have been created. We also utilize this database as a resource when formulating new diets. We will be pleased to provide you a complete formula sheet, containing the ingredients and inclusion rate, macronutrient values, key features, and planning and ordering information.

Purified diets use refined ingredients such as casein, sucrose, cornstarch, and cellulose. These human food grade ingredients have relatively simple chemical compositions (predominantly one nutrient classification) and this feature is important for manipulating individual nutrients for research purposes. Additionally, most refined ingredients contain very limited levels of non-nutrients that could have biological activity. This is in contrast to the natural ingredients (corn, wheat, soybean meal, etc.) used in standard diets, which have relatively complex chemical compositions as well as various phytochemicals that may or may not be physiologically relevant. Refined ingredients are obtained from reliable suppliers of human grade products, and we consistently obtain ingredients from the same vendors, year after year. Additionally, the major protein ingredients are assayed for a specific panel of nutrients to ensure minimal variation. Thus, purified diets will be repeatable and can easily be modified. Examples of common purified diets include AIN-76A (CA.170481), AIN-93G (TD.94045), and AIN-93M (TD.94048).

The American Society for Nutrition (ASN) (formerly American Institute of Nutrition, AIN) has promoted the use of nutritionally adequate purified diets for rodents, because many researchers may not be aware of potential nutritional variables. The AIN-76 formula was published in 1977, and became AIN-76A with minor revision (increased Vitamin K) in 1980. The diet has been used extensively, and there were several suggestions about improvements. This resulted in the publication of two formulas in 1993, AIN-93G (for growth, reproduction, and lactation) and AIN-93M (for maintenance). The article J. Nutr. 123: 1939-1951 (1993) discusses the rationale for the several revisions. Some of the changes include soybean oil rather than corn oil, the addition of other carbohydrate sources and lower sucrose, decreased phosphorus, cystine in place of methionine, inclusion of several trace mineral elements, and a few vitamin adjustments. The maintenance diet has less protein and fat.

Each formula is given a unique identification number and retains that identity among the thousands of formulas in our files. This assures the same formula will be used even years later. Upon production, a six or seven-digit “Rx” lot number is assigned that allows for traceability through all phases of diet production. These detailed production records are kept for every item produced. Quality-control samples are retained for nine months. Each lot of the major protein ingredients is analyzed for a number of nutrients to verify uniformity from lot to lot. Care is taken to retain reputable vendors for these refined ingredients.

Yes, we can help you in adding a test compound or ingredient to a diet. This is often a convenient approach to dosing research animals. When adding a compound to a diet, there are some important safety and stability considerations. Please see customer supplied ingredients for more specific information.

A phone call or an email exchange with one of our staff will augment the information that you gather from the web site or published literature, and you are more likely to receive a diet that best meets your particular needs. We are careful to maintain confidentiality, and will sign formal agreements if necessary. Please contact us at info@jinpanbio.com for consultation regarding your specific diet needs.

Advise us why the diet is needed, and for what animal species. Discuss the nutrients or ingredients of interest in as much detail as possible. Please avoid subjective descriptions such as “low, normal, and high” because these terms have different meanings to various researchers. Provide us with a copy of any existing formula to be duplicated or modified, and any journal article(s) upon which your research may be based. Let us know if the diet must be irradiated.

Our minimum order for custom research diets is 3 kg. This is particularly convenient when feeding small numbers of mice, especially for diet that contains your test compound. The minimum quantity for vitamin and mineral mixes is 500 g.

我们的饮食风俗研究最少是 3 公斤。这是特别方便的小数量喂养、饮食含有测试化合物。在最小量的维生素和矿物质混合料是 500 克。

Prices are dependent on formula details, ingredient composition, order quantity, and form (pellet or powder). If you know the product code, contact customer service at  info@jinpanbio.com. If you are unsure of the product code, have a new request, or require other technical information, contact a Nutritionist info@jinpanbio.com.

Standard packaging includes a small box (nine x nine x 11 inches) that will fit up to five kg, a medium box (ten x ten x 15 inches) that will fit up to ten kg, and a larger heavy-duty box (13 x 13 x 20 inches) with inset handles that holds up to 20 kg. Every container also includes a three-mil poly liner to keep your research diet as fresh as possible. Vacuum packaging and specific quantity packaging are also available at additional cost. Vacuum packaging will typically reduce the amount of diet fit into a box. Contact us at info@jinpanbio.com for more information.

Diets are custom made to fill each order. The typical lead-time between order and shipment is ten business days. The exceptions to this are TD.88137, TD.06414, and TD.01306 which for orders of 25 kg or less, are usually available within a week. If irradiation is required (add ten business days). We can often accommodate your rush production needs, for an additional fee. Contact us at info@jinpanbio.com for more details. Transit time depends on the type of delivery method used (regular ground, overnight, etc.). Weight and delivery method affect shipping costs, which are typically prepaid and added to the invoice.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. Custom research diets are perishable and should be refrigerated at 4 degrees celsius (40 degrees fahrenheit) or lower (see Fullerton, et.al. J Nutr. 112:567-573, 1982). As a general guideline, diets can be stored this way for up to six months. Freezing a diet may slow reactive processes, but there is not sufficient information available to recommend feeding a diet past six months as a general guideline. Use of diets beyond the recommended use date will be subject to the protocols of your institution.

没有一个人能够预测当特定的饲料会破坏或缺乏一个或更多的营养。饲料习俗研究很容易腐烂并冷藏在 4 ℃ 或更低 (参见 Fullerton 等。J Nutr 。112 : 567 – 573 , 1982) 。作为一般性指导 , 可这种方法存放六个月之久。冷冻的食物可减慢反应过程 , 但是没有足够的信息可用以推荐的饮食喂养 6 个月。使用饲料建议的使用日期的协议。

Custom research diets vary significantly in their formulation. There may be certain features of a diet that merit a shorter shelf-life. To learn more about storage and use recommendations, and to help you asess if your diet has features that may warrant a shorter shelf-life we have put together a guide. If you have further questions about your diet, contact us at info@jinpanbio.com.

Barrier facilities or certain animal models require sterilized diet. Most custom research diets do not withstand autoclaving; however, we can arrange for qualified diets to be irradiated. The irradiation dose range is two to five MRad, or 20-50 kGy. Nominal fees and extra time are involved. Please contact us at info@jinpanbio.com for details about irradiation costs and additional lead-time.

A nutritionist can assist you in determining if a formula is suitable for irradiation. Irradiation will lead to selective vitamin loss, and it is our practice to increase the inclusion rate of certain vitamin mixes to help ensure that the final diet contains sufficient vitamins. Irradiated diets are double-bagged. Vacuum packaging may be helpful in reducing irradiation-induced peroxide formation in high fat diets.

Some diet formulas are fairly common or generic, and probably are not considered confidential by those who use them, especially when authors provide a product number. Formulas might be difficult to trace without a product number, and then must be discussed with a nutritionist. We will review each formula for history and confidentiality.

Provide us with at the very least a description, preferably the complete formula, and we’ll give you an assessment of our ability to make that formula.

There are limitations for pelleting diets. The level and type of fat, the carbohydrate composition, and the interaction of fat and carbohydrate will impact our ability to pellet a diet. Consult a nutritionist at info@jinpanbio.com for more information about specific formulas.

“Vitamin-Free” Test Casein is an alcohol-extracted casein prepared from regular casein, and typically has 90-91% protein (%N x 6.38), 0.1% fat, and four-six % moisture. The alcohol extraction reduces fat, fat soluble vitamins, and some of the B vitamins. This protein source is most appropriately used in vitamin deficient diets, and when researchers want to minimize “background fat” in a diet. We have prepared this specialty ingredient for many years, and continue to supply it to many labs. We will develop special pricing on request for large quantities. Advance notice is recommended for orders of 1,000 Kg or more. If you need this ingredient, consider Envigo as your preferred source.

Yes. Some of the same high-quality ingredients used to produce custom research diets can also be supplied to those who prepare their own diets. See diet ingredients for more information.

When citing Teklad diets, include a description, and a product number — standard grain based diets are typically four numbers while custom diets usually start with “TD” followed by five or six numbers. Use the following examples as a guide:

“Rats were maintained on minimal phytoestrogen diet 2016 Teklad Global 16% Protein Rodent Diet, Envigo, Madison, Wisconsin USA, www.amrescoinc.cn”

“Obesity was induced by feeding Teklad high fat diet TD.06414 with 60% of kcal from fat, primarily lard, Envigo, Madison, Wisconsin USA, www.amrescoinc.cn”

“Mice were fed Teklad vitamin D deficient diet TD.89123, Envigo, Madison Wisconsin USA, www.amrescoinc.cn

Each diet has a unique product number that will allow others to quickly and easily obtain information about the diet used in your studies by contacting info@jinpanbio.com

No. According to the NIH OACU Guidelines for Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals (2008), the vehicle used to facilitate administration of a compound is as important of a consideration as the active compound in the preparation. Diet as a vehicle, either whole or as the sum of its parts, would not meet the standard for pharmaceutical grade as defined by this document.

You may have been asked this question by your IACUC due to a passage in the 8thedition of the Guide for the Care and Use of Laboratory Animals (2011) stating that pharmaceutical-grade chemicals and other substances should be used, when available, for all animal-related procedures (page 31). In general, deviations from “Must” or “Should” statements within the Guide require justification and approval by your institutional IACUC.

Alternative dosing techniques (gavage, injection) can introduce stress from handling and increase the risk for potential injury. Stress can affect animal well-being as well as scientific parameters through modifications in behavior and physiology. Dosing via diet is an established method for delivering test compounds to animals that is non-invasive, reducing stress and potential injury from handling. Therefore, use of non-pharmaceutical grade compounds as a delivery method is justified, as it is advantageous for both animal welfare and experimental design.

Further reading:

Guide for the Care and Use of Laboratory Animals: 8th Edition (2011). National Research Council Committee for the Update of the Guide for the Care and Use of Laboratory Animals.

Guidelines for the Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals. (2008). Animal Research Advisory Committee, Office of Animal Care and Use, NIH.

Laboratory routines cause animal stress. (2004). Balcombe, J.P., Barnard, N.D., and Sandusky, C. Contemp Top Lab Anim Sci 43, 42-51.

Harlan 动物饲料 TD.94049 AIN-93M Mineral Mix AIN-93M矿物质混合物

Harlan 动物饲料 TD.94049 AIN-93M Mineral Mix AIN-93M矿物质混合物
Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula g/Kg
Calcium Carbonate 357.0
Potassium Phosphate, monobasic 250.0
Potassium Citrate, monohydrate 28.0
Sodium Chloride 74.0
Potassium Sulfate 46.6
Magnesium Oxide 24.3
Ferric Citrate 6.06
Zinc Carbonate 1.65
Manganous Carbonate 0.63
Cupric Carbonate 0.31
Potassium Iodate 0.01
Sodium Selenate 0.0103
Ammonium Paramolybdate, tetrahydrate 0.008
Sodium Meta-Silicate, nonahydrate 1.45
Chromium Potassium Sulfate, dodecahydrate 0.275
Lithium Chloride 0.0174
Boric Acid 0.0815
Sodium Fluoride 0.0635
Nickel Carbonate Hydroxide, tetrahydrate 0.0318
Ammonium Meta-Vanadate 0.0066
Sucrose, fine ground 209.496

碳酸钙  357.0
磷酸二氢钾  250.0
一水柠檬酸钾 28.0
氯化钠  74.0
硫酸钾  46.6
氧化镁  24.3
柠檬酸铁  6.06
碳酸锌  1.65
碳酸锰  0.63
碳酸铜  0.31
碘酸钾  0.01
硒酸钠  0.0103
四水合低聚钼酸铵  0.008
非水合半硅酸钠  1.45
硫酸铬钾,十二水合物  0.275
氯化锂  0.0174
硼酸  0.0815
氟化钠  0.0635
碳酸镍四水合物  0.0318
偏钒酸铵  0.0066
蔗糖,细磨粉  209.496

Footnote
Reference: J. Nutr. 123:1939-1951, 1993. Supplies the recommended concentrations of mineral elements for AIN-93M diet, when mineral mix is used at 35 g/kg diet. Has more phosphorus than AIN-93G mineral mix.

参考:J. Nutr。 123:1939-1951,1993。当以35 g / kg的饮食使用矿物质混合物时,为AIN-93M饮食提供推荐的矿物质元素浓度。 比AIN-93G矿物混合物中的磷更多。

Harlan代理 TD.85419 Zinc Deficient Diet 锌缺乏饲料

Harlan代理 TD.85419 Zinc Deficient Diet 锌缺乏饲料
Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Harlan TD.85419  Zinc Deficient Diet

Formula g/Kg
Egg White Solids, spray-dried 200
Dextrose, monohydrate 634.2658
Corn Oil 100
Cellulose 30
Vitamin Mix, Teklad (40060) 10
Biotin 0.004
Mineral Mix, Zn Deficient (81264) 25.6902
Chromium Potassium Sulfate, dodecahydrate 0.02
Ethoxyquin, antioxidant 0.02

Footnote

Egg white solids are used instead of casein to reduce background zinc. The zinc content of the diet ranges from approximately 0.5 – 1.5 ppm. TD.85420 is a possible ingredient matched control with ~50ppm zinc from zinc carbonate. For questions about this or other zinc adjusted diets

Selected Nutrient Information

% by weight % kcal from
Protein 16.1 16.6
Carbohydrate 58.3 60.2
Fat 10 23.2
Kcal/g 3.9
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Zinc
  • Egg White Solids
  • Rodent

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Harlan代理 TD.80396 Iron Deficient Diet 缺铁饲料

Harlan代理 TD.80396 Iron Deficient Diet 缺铁饲料
Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Harlan TD.80396  Iron Deficient Diet

Formula g/Kg
Casein, low Cu & Fe 200
DL-Methionine 3
Sucrose 549.99
Corn Starch 150
Corn Oil 50
Mineral Mix, Fe Deficient (81062) 35
Vitamin Mix, AIN-76A (40077) 10
Choline Bitartrate 2
Ethoxyquin, antioxidant 0.01

Footnote

The diet contains approximately 2-6 ppm Fe. To limit background iron, cellulose is omitted, and reagent grade, pretested calcium phosphate is used in the mineral mix. TD.80394 is a possible ingredient matched control with ~48 ppm added iron from ferric citrate. For questions about this or other iron adjusted diets

Selected Nutrient Information1

% by weight % kcal from
Protein 17.7 17.8
Carbohydrate 69.9 70.4
Fat 5.2 11.8
Kcal/g 4
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • AIN-76A Modification
  • Iron Deficient

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation not advised
    • Contact a nutritionist for recommendations

Harlan 2020X Teklad Global Soy Protein-Free Extruded Rodent Diet饲料说明书

Harlan 2020X Teklad Global Soy Protein-Free Extruded Rodent Diet饲料说明书

Teklad Global Soy Protein-Free Extruded Rodent Diet

Product Description- 2020X is a fixed formula, non-autoclavable extruded diet manufactured with high quality ingredients and designed to support gestation, lactation, and growth of rodents. 2020X does not contain alfalfa or soybean meal, thus minimizing the occurrence of natural phytoestrogens. Typical isoflavone concentrations (daidzein + genistein agylcone equivalents) range from non-detectable to 20 mg/kg. Exclusion of alfalfa reduces chlorophyll, improving optical imaging clarity. Absence of animal protein and fish meal minimizes the presence of nitrosamines. Also available certified (2020XC) and irradiated (2920X). For autoclavable diet, refer to 2020SX (Sterilizable).

Teklad Global 无大豆蛋白膨化啮齿动物饮食

产品描述- 2020X 是一种固定配方、非高压灭菌的膨化饮食,采用优质成分制造,旨在支持啮齿动物的妊娠、哺乳和生长。 2020X 不含苜蓿或豆粕,从而最大限度地减少天然植物雌激素的出现。 典型的异黄酮浓度(黄豆苷元 + 染料木黄酮当量)范围从不可检测到 20 mg/kg。 排除紫花苜蓿可减少叶绿素,提高光学成像清晰度。 不含动物蛋白和鱼粉可最大限度地减少亚硝胺的存在。 也可提供认证 (2020XC) 和辐照 (2920X)。 有关可高压灭菌的饮食,请参阅 2020SX(可灭菌)。

Macronutrients    
Crude Protein % 19.1
Fat (acid hydrolysis) a % 6.5
Carbohydrate (available) b % 47.0
Crude Fiber % 2.7
Neutral Detergent Fiber c % 12.3
Ash % 5.1
Energy Density d kcal/g (kJ/g) 3.1 (13.0)
Calories from Protein % 24
Calories from Fat % 16
Calories from Carbohydrate % 60
Minerals    
Calcium % 0.9
Phosphorus % 0.7
Non-Phytate Phosphorus % 0.4
Sodium % 0.1
Potassium % 0.4
Chloride % 0.4
Magnesium % 0.2
Zinc mg/kg 60
Manganese mg/kg 80
Copper mg/kg 15
Iodine mg/kg 6
Iron mg/kg 200
Selenium mg/kg 0.23
Amino Acids
Aspartic Acid % 1.1
Glutamic Acid % 3.5
Alanine % 1.2
Glycine % 0.7
Threonine % 0.6
Proline % 1.9
Serine % 0.9
Leucine % 2.3
Isoleucine % 0.7
Valine % 0.9
Phenylalanine % 1
Tyrosine % 0.5
Methionine % 0.5
Cystine % 0.3
Lysine % 0.9
Histidine % 0.4
Arginine 0.8
Tryptophan % 0.2

Ingredients (in descending order of inclusion)- Ground wheat, ground corn, corn gluten meal, wheat middlings, soybean oil, calcium carbonate, dicalcium phosphate, brewers dried yeast, L-lysine, iodized salt, magnesium oxide, choline chloride, DL-methionine, calcium propionate, L-tryptophan, vitamin E acetate, menadione sodium bisulfite complex (source of vitamin K activity), manganous oxide, ferrous sulfate, zinc oxide, niacin, calcium pantothenate, copper sulfate, pyridoxine hydrochloride, riboflavin, thiamin mononitrate, vitamin A acetate, calcium iodate, vitamin B12 supplement, folic acid, biotin, vitamin D3 supplement, cobalt carbonate.

Vitamins  
Vitamin A e, f IU/g 15.0
Vitamin D3 e, g IU/g 1.5
Vitamin E IU/kg 110
Vitamin K3 (menadione) mg/kg 50
Vitamin B (thiamin) mg/kg 17
Vitamin B2 (riboflavin) mg/kg 15
Niacin (nicotinic acid) mg/kg 75
Vitamin B6 (pyridoxine) mg/kg 18
Pantothenic Acid mg/kg 33
Vitamin B12 (cyanocobalamin) mg/kg 0.08
Biotin mg/kg 0.40
Folate mg/kg 4
Choline mg/kg 1200
Fatty Acids  
C16:0 Palmitic % 0.6
C18:0 Stearic % 0.1
C18:1ω9 Oleic % 1.1
C18:2ω6 Linoleic % 2.6
C18:3ω3 Linolenic % 0.3
Total Saturated % 0.8
Total Monounsaturated % 1.1
Total Polyunsaturated % 2.9
Other  
Cholesterol mg/kg

 

Ether extract is used to measure fat in pelleted diets, while an acid hydrolysismethod is require d to recover fat in extruded diets.Compared to ether
extract, the fat value for acid hydrolysis will be approximately 1% point higher.
b Carbohydrate (available) is calculated by subtracting neutral detergent fiberfrom total carbohydrates.
Neutral detergent fiber is an estimate ofinsoluble fiber,including cellulose,hemicellulose, and lignin.Crude fiber methodology underestimates total fiber.dEnergy density is a calculated estimate ofmetabolizable energy based on theAtwater factors assigning 4 kcal/g to protein,9 kcal/gto fat, and 4 kcal/g toavailable carbohydrate.
In dicates added amount but does not account for contribution from otheringredients.
f1IU vitamin A = 0.3 ug retinol
g 1 lU vitamin D= 25 ng cholecalciferol
Fornutrients not listed, insufficient data is available to quantify.
Nutrient data represent the best information available, calculated frompublished values and direct analytical testing of raw materials and finishedproduct. Nutrient values may vary due to the natural variations in theingredients , analysis,and effects of processing.

乙醚提取物用于测量颗粒饲料中的脂肪,而酸水解法则需要回收膨化饲料中的脂肪。与乙醚相比
提取物,酸水解的脂肪值将高出约 1%。
b 碳水化合物(可用)通过从总碳水化合物中减去中性洗涤纤维计算得出。
中性洗涤剂纤维是不溶性纤维的估计值,包括纤维素、半纤维素和木质素。粗纤维方法低估了总纤维。d 能量密度是基于 Atwater 因子计算的代谢能估计值,将 4 kcal/g 分配给蛋白质,9 kcal/g 分配给脂肪,和 4 kcal/g 的可利用碳水化合物。
表示添加量,但不考虑其他成分的贡献。
f1IU 维生素 A = 0.3 ug 视黄醇
g 1 lU 维生素 D= 25 ng 胆钙化醇
对于未列出的营养素,没有足够的数据来量化。
营养数据代表了可用的最佳信息,根据公布的值和原材料和成品的直接分析测试计算得出。由于成分、分析和加工效果的自然变化,营养价值可能会有所不同。 Harlan

Harlan 2020X Teklad global soy protein-free extruded rodent diets饲料

Harlan 2020X Teklad global soy protein-free extruded rodent diets饲料

Product Features

  • Designed to support gestation, lactation, and growth of rodents
  • Formulated to exclude soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from non-detectable to 20 mg/kg
  • Exclusion of alfalfa reduces chlorophyll, greatly improving fluorescent optical imaging  clarity
  • Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)

产品特点

旨在支持啮齿动物的妊娠、哺乳和生长
配方不含豆粕,从而最大限度地减少异黄酮的存在,异黄酮是实验室动物饮食中发现的主要植物雌激素类型
典型的异黄酮浓度(黄豆苷元 + 金雀异黄苷元当量)范围从不可检测到 20 mg/kg
排除苜蓿可减少叶绿素,大大提高荧光光学成像清晰度
不含动物蛋白和鱼粉可最大限度地减少亚硝胺(一种潜在的致癌物质)的存在

Products

TEKLAD RODENT DIETS NON-AUTOCLAVABLE FORM AUTOCLAVABLE IRRADIATED
Teklad global soy protein-free extruded 2020X  2020SX  2920X 

Technical resources
Teklad Global Diets® product line
Teklad global rodent diets quality and scientific support
Rodent diet and ingredient comparison
Teklad certified diet sheet
Teklad irradiated diet sheet
Diet impacts fluorescent imaging sheet

Harlan TD.10885 45% Fat Kcal Diet (0.2% TC)高脂饲料成分表

Harlan TD.10885 45% Fat Kcal Diet (0.2% TC)高脂饲料成分表

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料
Harlan Teklad动物饲料
Harlan代理
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula   g/Kg
Casein   195.0
L-Cystine   3.0
Sucrose   340.0
Corn Starch   55.46
Maltodextrin   60.0
Anhydrous Milkfat   210.0
Cholesterol   1.5
Soybean Oil   20.0
Cellulose   50.0
Mineral Mix, AIN-93G-MX (94046)   43.0
Vitamin Mix, AIN-93-VX (94047)   19.0
Choline Bitartrate   3.0
TBHQ, antioxidant   0.04
Footnote
Designed with similarities to TD.88137 Western Diet with 21% anhydrous milk fat and 34% sucrose. 2% soybean oil is included to supplement essential fatty acids. Modified corn starch (maltodextrin) is added for improved pellet quality. Total cholesterol of 0.2%.
Selected Nutrient Information1
  % by weight % kcal from
Protein 17.3 14.8
Carbohydrate 47.5 40.6
Fat 23.2 44.6
Kcal/g 4.7

1 Values are calculated from ingredient analysis or manufacturer data

Key Features
Purified Diet

Modification of TD.88137

Improved Pellet Quality

Improved Essential Fatty Acids

Key Planning Information
Products are made fresh to order

+ Store product at 4°C or lower

+ Use within 6 months (applicable to most diets)

+ Box labeled with product name, manufacturing date, and lot number

+ Replace diet at minimum once per week

More frequent replacement may be advised

+ Lead time:

· 2 weeks non-irradiated

· 4 weeks irradiated

Harlan TD.07864 1% Choline Diet (2018)饲料成分表

Harlan TD.07864 1% Choline Diet (2018)饲料成分表

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询上海金畔生物获取更多详细资料。

The TD.130328 is the replacement for TD.07864

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 988.2
Choline Chloride, customer supplied 11.8

Footnote

The 2018 Teklad Global diet with 1% added choline from choline chloride (approx. 85% choline).

Selected Nutrient Information1

  % by weight % kcal from
Protein 18.0 22.9
Carbohydrate2 47.4 60.5
Fat 5.8 16.6
Kcal/g 3.1  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Choline
  • Customer Supplied Ingredient
  • Teklad 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。