Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

上海金畔生物代理Lumiprobe品牌全线产品,欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

Lumiprobe protein labeling kits contain pre-aliquoted reactive dye, all necessary auxiliary components and buffers, and desalting spin columns to achieve efficient, reproducible labeling of antibodies, and other proteins.

The labeled protein is purified by means of gel filtration on gel filled spin columns that are enclosed. This protocol ensures complete removal of the unbound dye, and excellent recovery of the labeled product. The protein is eluted in PBS buffer, ready for the use in most downstream applications.

The protocol is quick, effortless, and easy to follow.

Lumiprobe 探针蛋白标记试剂盒包含预先引用的活性染料、所有必需的辅助成分和缓冲器,以及脱盐自旋柱,以实现抗体和其他蛋白质的高效、可复制的标记。

标记蛋白是通过凝胶过滤纯化的凝胶填充自旋柱是封闭的。该协议确保完全去除未结合染料,并出色地恢复标记产品。该蛋白在PBS缓冲液中被洗脱,可以在大多数下游应用中使用。

该试剂盒具有快速,毫不费力,易于遵循等优点。

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.


sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.


sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.


sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.


sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.

Lumiprobe 23420 BDP TR NHS ester

Lumiprobe 23420 BDP TR NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

BDP TR is a bright and photostable borondipyrromethene dye, which is especially suitable for microscopy applications. The dye has a long excited state lifetime, and is therefore is useful for fluorescence polarization assays. Its large two-photon cross section makes this dye useful for two-photon spectroscopy.

This NHS ester is an amine-reactive form of the dye.

Lumiprobe 23420 BDP TR NHS ester

Cat. # Quantity Lead time
13420 1 mg in stock
23420 5 mg in stock
43420 25 mg in stock
53420 50 mg in stock
63420 100 mg in stock

BDP TR NHS ester structure

Absorption and emission spectra of BODIPY TR

Absorption and emission spectra of BODIPY TR

General properties

Appearance: dark blue-black crystals
Mass spec M+ increment: 406.1
Molecular weight: 521.30
Molecular formula: C25H18BF2N3O5S
IUPAC name: Methyl {p-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]phenoxy}acetate
Quality control: HPLC-MS (95%), UV-Vis
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 589
ε, L⋅mol−1⋅cm−1: 69000
Emission maximum, nm: 616
Fluorescence quantum yield: 0.9
CF260: 0.15
CF280: 0.19

Lumiprobe Bodipy(BDP)活性染料产品列表

BDP 558/568 NHS ester

BDP 581/591 NHS ester

BDP 630/650 X NHS ester

BDP FL NHS ester

BDP R6G NHS ester

BDP TMR NHS ester

BDP TR NHS ester

BDP 558/568 azide

BDP 581/591 azide

BDP 630/650 azide

BDP FL azide

BDP R6G azide

BDP TMR azide

BDP TR azide

BDP 558/568 alkyne

BDP 581/591 alkyne

BDP 630/650 alkyne

BDP FL alkyne

BDP R6G alkyne

BDP TMR alkyne

BDP TR alkyne

BDP 581/591 maleimide

BDP 630/650 maleimide

BDP FL maleimide

BDP R6G maleimide

BDP TMR maleimide

BDP TR maleimide

BDP 581/591 hydrazide

BDP 630/650 hydrazide

BDP FL hydrazide

BDP TR hydrazide

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 581/591 tetrazine

BDP 630/650 tetrazine

BDP FL tetrazine

BDP TR tetrazine

BDP 581/591 DBCO

BDP FL DBCO

BDP R6G DBCO

BDP TR methyl ester, 5 mM in DMSO

Lumiprobe 26420 BDP 581/591 NHS ester

Lumiprobe 26420 BDP 581/591 NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe 26420 BDP 581/591 NHS ester

BDP 581/591 is a borondipyrromethene dye that has relatively long fluorescence lifetime and two photon excitation cross section. It is useful for fluorescence polarization assays. Due to the presence of a diene system, it can react with reactive oxygen species (ROS) with a change of fluorescence.

This is the NHS ester derivative for the conjugation with primary and secondary amine groups of proteins, peptides, and other molecules.

Cat. # Quantity Lead time
16420 1 mg in stock
26420 5 mg in stock
46420 25 mg in stock
56420 50 mg in stock
66420 100 mg in stock

 

BDP 581/591 NHS ester

Structure of BDP 581/591 NHS ester

Absorption and emission spectra of BDP 581/591

Absorption and emission spectra of BDP 581/591

General properties

Appearance: dark colored solid
Mass spec M+ increment: 374.1
Molecular weight: 489.28
Molecular formula: C26H22N3BF2O4
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

Spectral properties

Excitation maximum, nm: 585
Emission maximum, nm: 594
CF260: 0.06
CF280: 0.04

Product citations

  1. Tabe, H.; Sukenobe, K.; Kondo, T.; Sakurai, A.; Maruo, M.; Shimauchi, A.; Hirano, M.; Uno, S.-N.; Kamiya, M.; Urano, Y.; Matsushita, M.; Fujiyoshi, S. Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix. The Journal of Physical Chemistry B, 2018, 122(27), 6906–6911. doi: 10.1021/acs.jpcb.8b03977

 

Lumiprobe Bodipy(BDP)活性染料产品列表

Lumiprobe Bodipy(BDP)活性染料产品列表

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe Bodipy(BDP)活性染料产品列表

BDP 558/568 NHS ester

BDP 581/591 NHS ester

BDP 630/650 X NHS ester

BDP FL NHS ester

BDP R6G NHS ester

BDP TMR NHS ester

BDP TR NHS ester

BDP 558/568 azide

BDP 581/591 azide

BDP 630/650 azide

BDP FL azide

BDP R6G azide

BDP TMR azide

BDP TR azide

BDP 558/568 alkyne

BDP 581/591 alkyne

BDP 630/650 alkyne

BDP FL alkyne

BDP R6G alkyne

BDP TMR alkyne

BDP TR alkyne

BDP 581/591 maleimide

BDP 630/650 maleimide

BDP FL maleimide

BDP R6G maleimide

BDP TMR maleimide

BDP TR maleimide

BDP 581/591 hydrazide

BDP 630/650 hydrazide

BDP FL hydrazide

BDP TR hydrazide

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 581/591 tetrazine

BDP 630/650 tetrazine

BDP FL tetrazine

BDP TR tetrazine

BDP 581/591 DBCO

BDP FL DBCO

BDP R6G DBCO

BDP TR methyl ester, 5 mM in DMSO

Lumiprobe BDP FL NHS ester

Lumiprobe BDP FL NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe BDP FL NHS ester

Cat. # Quantity Lead time
11420 1 mg in stock
21420 5 mg in stock
41420 25 mg in stock
51420 50 mg in stock
61420 100 mg in stock

BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein, a molecule identical to BODIPY FL® NHS ester. An amino-reactive dye for the labeling of proteins and peptides.

While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY-FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.

The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.

The dye is a good replacement for fluorescein (FAM), BODIPY-FL, Alexa Fluor 488, DyLight 488, Cy2, and other 488 nm dyes.

BDP FL NHS ester

Photo of BDP FL solution

Structure of BODIPY FL NHS ester

Absorption and emission spectra of BDP FL

Absorption and emission spectra of BDP FL

General properties

Appearance: orange solid
Mass spec M+ increment: 274.1
Molecular weight: 389.16
CAS number: 146616-66-2
Molecular formula: C18H18BF2N3O4
Solubility: Good in organic solvents (DMF, DMSO, dichlromethane), limited in water
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

Product citations

  1. Poreba, M.; Rut, W.; Vizovisek, M.; Groborz, K.; Kasperkiewicz, P.; Finlay, D.; Vuori, K.; Turk, D.; Turk, B.; Salvesen, G.; Drag, M.Selective imaging of human cathepsin L in breast cancer by fluorescent activity-based probes. Chemical Science, 2018, 9(8), 2113–2129. doi: 10.1039/C7SC04303A
  2. Grube, L.; Dellen, R.; Kruse, F.; Schwender, H.; Stuehler, K.; Poschmann, G. Mining the secretome of C2C12 muscle cells: Data dependent experimental approach to analyze protein secretion using label-free quantification and peptide based analysis. Journal of Proteome Research, 2018, 17(2), 879–890. doi: 10.1021/acs.jproteome.7b00684
  3. Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  4. Gaspar, I.; Wippich, F.; Ephrussi, A. Enzymatic production of single molecule FISH and RNA capture probes. RNA, 2017, 23(10), 1582–1591. doi: 10.1261/rna.061184.117

Bodipy(BDP)氟硼荧光活性染料发展历史

Bodipy二吡咯类荧光染料相对比于荧光素、罗丹明或者菁染料有着较高的选择性、高灵敏度、高荧光量子产率、高摩尔消光系数、良好的光学稳定性和对PH不敏感的优点被广泛应用于生物标记、荧光探针及生物成像,西安瑞禧生物科技有限公司可以提供种类丰富的Bodipy荧光染料,荧光波长可定制 从550-800之间,末端可以修饰各种活性基团。

Bodipy类荧光染料具有良好的稳定性、优良的光学性能,主要表现在以下几个方面:

1:BODIPY的光谱峰宽比较窄,在光学领域和生物学领域进行分析检测时,光谱峰宽较窄,检测灵敏度较高,并且减少了待测样品的用量。测得的结果中,荧光光谱上会出现更为明显的荧光吸收和发射的变化,这样更精确地测得了样品的含量。

2:在整个BODIPY分子中,并不存在负电荷。因而在溶液中,溶液中的离子无法与缺少电荷的BODIPY分子发生静电作用,这样非常有效的避免了测定时静电作用对测量结果的干扰,使测定过程更加稳定,测得结果更加精准。

3:BODIPY类荧光染料的光稳定性能非常优越,当它被激发到达激发态时,不会因为很长时间的激发而在分析测定过程中发生激发态的分解或其他变化,这样测得的荧光光谱更加准确可信。

4:BODIPY染料,其荧光量子产率甚至接近1.0。一般普通的染料在水溶液中其荧光量子产率会变低,有的甚至被猝灭。对于BODIPY分子来说,在水溶液中其荧光量子产率虽然有所下降,但它仍然能保持较高的荧光量子产率。

5:由BODIPY的母体结构可知,他的母体结构上的取代基一般不会出现氨基或羧基这类对酸碱敏感的活性基团。因此外界的酸碱度对其影响会大幅度减小,因此BODIPY染料不会轻易变质。

6:BODIPY类荧光染料对光的感应度很高,并且它还有高的摩尔消光系数,有些经过修饰后的BODIPY染料在水溶液中的溶解度也较高,再加上它在水中不易被猝灭这一优异的性能,使得BODIPY在光学、生物学、分析学领域有着极其广泛的应用。

西安瑞禧生物科技有限公司可以提供激发和发射波长从500nm-800nm的活性染料(包括800nm左右的近红外)并且可以修饰NHS、MAL、NH2、COOH、N3、SH等不同活性基团。

常规的Bodipy荧光染料都是激发发射波长都是在500-650nm之间的,包括国外的商家也只能提供500-650nm波长的荧光染料,瑞禧生物不仅可以提供波长在500-600nm之间的波长 还可以提供650-800nm之间的近红外波段的BDP

500-800之间的波长可以选择:
Bodipy carboxylic acid(羧基COOH)
Bodipy NHS ester(活化脂NHS)
Bodipy amine (NH2氨基)
Bodipy maleimide (MAL马来酰亚胺)
Bodipy azide (N3叠氮)
Bodipy alkyne (ALK炔烃)
Bodipy hydrazide (HZ酰基)
Bodipy Thiol (HS巯基)

产品列表:
BODIPY™ FL NHS Ester (Succinimidyl Ester)
BODIPY™ FL Sulfonated Succinimidyl Ester
BODIPY™ FL-X NHS Ester (Succinimidyl Ester)
BODIPY™ TMR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 530/550 NHS Ester (Succinimidyl Ester)
BODIPY™ R6G NHS Ester (Succinimidyl Ester)
BODIPY™ 581/591 NHS Ester (Succinimidyl Ester)
BODIPY™ 576/589 NHS Ester (Succinimidyl Ester)
BODIPY™ 650/665-X NHS Ester (Succinimidyl Ester)
BODIPY™ 493/503 NHS Ester (Succinimidyl Ester)
BODIPY™ 558/568 NHS Ester (Succinimidyl Ester)
BODIPY™ TR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 630/650-X NHS Ester (Succinimidyl Ester)
BODIPY™ FL maleimide
BODIPY™ FL azide
BODIPY™ FL alkyne
BODIPY™ FL hydrazide
BODIPY™ FL amine
BODIPY™ FL Carboxylic acid
BODIPY™ TR ceramide
BODIPY™ FL STP ester
BODIPY™ FL C12-sphingomyelin
BODIPY™ R6G NHS Ester
BODIPY™ 558/568 phalloidin
BODIPY™ FL hydrazide
BODIPY™ TMR-X NHS Ester
BODIPY™ FL L-cystine
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid

BODIPY®493/503 NHS ester
BODIPY®493/503 Carboxylic acid
BODIPY®493/503 amine
BODIPY®493/503 maleimide
BODIPY®493/503 azide
BODIPY®493/503 alkyne

BODIPY®558/568 NHS ester
BODIPY®558/568 Carboxylic acid
BODIPY®558/568 amine
BODIPY®558/568 maleimide
BODIPY®558/568 azide
BODIPY®558/568 alkyne

BODIPY®650/665 NHS ester
BODIPY®650/665 Carboxylic acid
BODIPY®650/665 amine
BODIPY®650/665 maleimide
BODIPY®650/665 azide
BODIPY®650/665 alkyne

BODIPY FL NHS Ester
BODIPY TMR NHS Ester
BODIPY TR NHS Ester
BODIPY FL amine
BODIPY TMR amine
BODIPY TR amine

 
以下染料的激发波长是685nm 发射波长是720nm
Azo-Bodipy-685  (ABDP685)
Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 NHS ester
Sulfo-Azo-Bodipy 685 NHS ester
Azo-Bodipy 685 amine
Sulfo-Azo-Bodipy 685 amine
PEG-Azo-Bodipy 685 NHS ester
PEG- Azo-Bodipy 685 amine
Azo-Bodipy 685 alkyne
Sulfo-Azo-Bodipy 685 alkyne
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Azo-Bodipy 685 maleimide
Sulfo-Azo-Bodipy 685 maleimide
Azo-Bodipy 685 pH6.5

我们还接受激发波长和发射波长在500nm-800nm之间的BDP活性荧光染料的定制产品。
上面所述染料可以连接:COOH,  NH2,  NHS,  MAL,  N3,  ALK,  hydrazide,  SH

其他波长的BDP活性染料的产品列表:
产品列表:
BDP 558/568 NHS ester
BDP 581/591 NHS ester
BDP 630/650 X NHS ester
BDP FL NHS ester
BDP R6G NHS ester
BDP TMR NHS ester
BDP TR NHS ester

BDP 581/591 azide
BDP 630/650 azide
BDP FL azide
BDP R6G azide
BDP TMR azide
BDP TR azide

BDP 558/568 alkyne
BDP 581/591 alkyne
BDP 630/650 alkyne
BDP FL alkyne
BDP R6G alkyne
BDP TMR alkyne
BDP TR alkyne

BDP 581/591 maleimide
BDP 630/650 maleimide
BDP FL maleimide
BDP R6G maleimide
BDP TMR maleimide
BDP TR maleimide

BDP 581/591 hydrazide
BDP 630/650 hydrazide
BDP FL hydrazide
BDP TR hydrazide

BDP 558/568 carboxylic acid
BDP 581/591 carboxylic acid
BDP 630/650 carboxylic acid
BDP FL carboxylic acid
BDP R6G carboxylic acid
BDP TMR carboxylic acid
BDP TR carboxylic acid

BDP 581/591 amine
BDP 630/650 amine
BDP FL amine
BDP R6G amine
BDP TMR amine
BDP TR amine

BDP 581/591 tetrazine
BDP 630/650 tetrazine
BDP FL tetrazine
BDP TR tetrazine

BODIPY母体结构最初是由德国化学家Treibs, A.和kreuzer, F. H.在1968年合成的,他们合成的BODIPY 类荧光染料 [40] 。另外,他们还合成了BODIPY染料的母体结构。在BODIPY母体结构中,Treibs, A.和kreuzer, F. H.在R1-R7中用不同基团取代得到不同的衍生产物。人们根据Treibs, A.和kreuzer, F. H.的前期探索,对BODIPY类染料做出了多种结构修饰,这大大丰富了BODIPY的种类,使其迅猛发展。

Lumiprobe cy7.5马来酰亚胺|Cyanine7.5 maleimide

Lumiprobe cy7.5马来酰亚胺|Cyanine7.5 maleimide

  • 英文名:Cyanine7.5 maleimide
  • 级别:NMR 1H (95%) and 13C, TLC, functional testing
  • 分子式:C51H55ClN4O3
  • 分子量:807.46
  • 品牌:lumiprobe
  • 外观:green powder
订购货号 产品名称及规格
16080 Cyanine7.5 maleimide, 1 mg
26080 Cyanine7.5 maleimide, 5 mg
46080 Cyanine7.5 maleimide, 25 mg
56080 Cyanine7.5 maleimide, 50 mg
66080 Cyanine7.5 maleimide, 100 mg

硫醇活性染料、近红外Cyanine7.5,用于标记蛋白的巯基基团,是Cy7.5® maleimide的类似物。
大多数蛋白含有巯基基团,可以选择性的标记马来酰亚胺。该试剂可以使这些蛋白连接上近红外荧光团。然后,该复合物可以用于非入侵性的活体成像,来研究组织和生物体内的蛋白分布。

推荐手册:

蛋白与其他硫醇分子的马来酰亚胺标记

Cy® is a trademark of GE Healthcare.

General properties

Appearance: >green powder
Molecular weight: >807.46
Molecular formula: C51H55ClN4O3
Solubility: >soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: >NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 788
Extinction coefficient at excitation maximum, Lmol-1cm-1: 223000
Emission maximum, nm: 808

Lumiprobe Cy7.5-N-羟基琥珀酰亚胺酯

Lumiprobe Cy7.5-N-羟基琥珀酰亚胺酯

  • 英文名:Cyanine7.5 NHS ester
  • 分子式:C49H52ClN3O4
  • 分子量:782.41
  • 品牌:lumiprobe
  • 外观:green powder
订购货号 产品名称及规格
16020 Cyanine7.5 NHS ester, 1 mg
26020 Cyanine7.5 NHS ester, 5 mg
46020 Cyanine7.5 NHS ester, 25 mg
56020 Cyanine7.5 NHS ester, 50 mg
66020 Cyanine7.5 NHS ester, 100 mg

Cyanine7.5是一种近红外荧光染料,具有很长的发射光谱,是此类荧光衍生而来的活性胺产品。近红外照射很容易穿透组织因此常应用于体内成像领域。该产品可以用于标记含有氨基的小分子物质,比如蛋白和多肽,通过近红外光谱成像技术(NIR imaging)来示踪他们在有机体内的分布。中心聚亚甲基基团的增强的稳定性使得量子产率较其母结构Cy7.5® NHS提高20%。

Cy® is a trademark of GE Healthcare.
General properties

Appearance: green powder
Molecular weight: 782.41
Molecular formula: C49H52ClN3O4
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 788
Extinction coefficient at excitation maximum, Lmol-1cm-1: 223000
Emission maximum, nm: 808

Product citations

  • Zhang, Z.; Cai, H.; Liu, Z.; Yao, P. Effective Enhancement of Hypoglycemic Effect of Insulin by Liver-targeted Nanoparticles Containing Cholic Acid-modified Chitosan Derivative. Molecular Pharmaceutics, in press. doi:10.1021/acs.molpharmaceut.6b00188
  • Dou, Y.; Guo, J.; Chen, Y.; Han, S.; Xu, X.; Shi, Q.; Jia, Y.; Liu, Y.; Deng, Y.; Wang, R.; Li, X.; Zhang, J. Sustained delivery by a cyclodextrin material-based nanocarrier potentiates antiatherosclerotic activity of rapamycin via selectively inhibiting mTORC1 in mice. Journal of Controlled Release, 2016, 235, 48–62. doi: 10.1016/j.jconrel.2016.05.049
  • Markovic, S.; Belz, J.; Kumar, R.; Cormack, R.A.; Sridhar, S.; Niedre, M. Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants. International Journal of Nanomedicine, 2016, 11, 1213–1223. doi: 10.2147/IJN.S93324
  • Han, F.Y.; Thurecht, K.J.; Lam, A.-L.; Whittaker, A.K.; Smith, M.T. Novel Polymeric Bioerodable Microparticles for Prolonged-Release Intrathecal Delivery of Analgesic Agents for Relief of Intractable Cancer-Related Pain. Journal of Pharmaceutical Sciences, 2015, 104(7), 2334–2344. doi: 10.1002/jps.24497
  • Duong, H.T.T.; Dong, Z.; Su, L; Boyer, C.; George, J.; Davis, T.P.; Wang, J. The Use of Nanoparticles to Deliver Nitric Oxide to Hepatic Stellate Cells for Treating Liver Fibrosis and Portal Hypertension. Small, 2015, 11(19), 2291–2304.201402870
  • Chen, K.; Xu, X.; Guo, J.W.; Zhang, X.; Han, S.; Wang, R.; Li, X.; Zhang, J. Enhanced Intracellular Delivery and Tissue Retention of Nanoparticles by Mussel-Inspired Surface Chemistry. Biomacromolecules, 2015, 16(11), 3574–3583. doi:10.1021/acs.biomac.5b01056
  • Lin, Y.-A.; Cheetham, A.G.; Zhang, P.; Ou, Y.-C.; Li, Y.; Liu, G.; Hermida Merino, D.; Hamley, I.W.; Cui, H. Multi-Walled Nanotubes Formed by Catanionic Mixtures of Drug Amphiphiles. ACS Nano, 2014, 8(12), 12690-12700. doi: 10.1021/nn505688b
  • Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  • Lu, H.D.; Soranno, D.E.; Rodell, C.B.; Kim, I.L.; Burdick, J.A. Secondary Photocrosslinking of Injectable Shear-Thinning Dock-and-Lock Hydrogels. Advanced Healthcare Materials, 2013, 2(7), 1028-1036. doi: 10.1002/adhm.201200343
  • Yang, H.; Mao, H.; Wan, Z.; Zhu, A.; Guo, M.; Li, Y.; Li, X.; Wan, J.; Yang, X.; Shuai, X. et al. Micelles assembled with carbocyanine dyes for theranostic near-infrared fluorescent cancer imaging and photothermal therapy. Biomaterials, 2013, 34(36), 9124-9133. doi: 10.1016/j.biomaterials.2013.08.022

Lumiprobe 辅助试剂Auxiliary reagents

Lumiprobe 辅助试剂Auxiliary reagents

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺点击化学用染料其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

辅助试剂 Auxiliary reagents
  • 标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.

  • 抗坏血酸 Ascorbic acid
ascorbic acid for reduction of Cu(II)-TBTA complex.
  • -TBTA络合物,10mM溶于55%的二甲亚砜水溶液

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

产品列表

中文名 货号 产品描述
Cy2-N-羟基琥珀酰亚胺酯 1A041 Cyanine2 NHS ester minimal dye, 5 nmol
抗坏血酸 12050 Ascorbic acid, 10 mg
铜-TBTA络合物,10mM溶于55%的二甲亚砜水溶液 21050 Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO, 1.5 mL
标记级别二甲基酰胺 13050 DMF (dimethylformamide), labeling grade, 1 mL
* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe DNA专用染料-亚磷酰胺 Phosphoramidites

Lumiprobe DNA专用染料-亚磷酰胺 Phosphoramidites

Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

亚磷酰胺 Phosphoramidites
炔烃亚酰胺 羟基脯氨醇 Alkyne Amidite, hydroxyprolinol

Acetylenic amidite for the incorporation of acetylene groups into oligos.

Alkyne Amidite, hydroxyprolinol
炔烃亚磷酰胺,5′-端基 Alkyne Phosphoramidite, 5′-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.

Alkyne Phosphoramidite, 5'-terminal
DMS(O)MT aminolink C6 DMS(O)MT aminolink C6

An amidite for the introduction of amino group.

DMS(O)MT aminolink C6
FAM 亚磷酰胺,6-异构体 FAM Phosphoramidite, 6-Isomer

Single isomer 6-FAM phosphoramidite for the synthesis of modified oligonucleotides.

FAM Phosphoramidite, 6-Isomer
Long trebler 亚磷酰胺 Long trebler phosphoramidite

Long trebler phosphoramidite is a branching reagent for DNA synthesis, allowing to prepare branched oligonucleotides, and dendrimers.

Long trebler phosphoramidite
芘 亚磷酰胺 dU Pyrene phosphoramidite dU

Pyrene phosphoramidite with pyrene attached to deoxyuridine.

Pyrene phosphoramidite dU

产品列表

货号
中文名
产品描述
21360 炔烃亚酰胺 羟基脯氨醇 Alkyne Amidite, hydroxyprolinol, 250 mg
41360 炔烃亚酰胺 羟基脯氨醇 Alkyne Amidite, hydroxyprolinol, 1 g
22260 炔烃亚磷酰胺,5′-端基 Alkyne Phosphoramidite, 5′-terminal, 250 mg
42260 炔烃亚磷酰胺,5′-端基 Alkyne Phosphoramidite, 5′-terminal, 1 g
62260 炔烃亚磷酰胺,5′-端基 Alkyne Phosphoramidite, 5′-terminal, 5 g
23260 DMS(O)MT aminolink C6 DMS(O)MT aminolink C6, 250 mg
43260 DMS(O)MT aminolink C6 DMS(O)MT aminolink C6, 1 g
63260 DMS(O)MT aminolink C6 DMS(O)MT aminolink C6, 5 g
C5160 FAM 亚磷酰胺,6-异构体 FAM Phosphoramidite, 6-Isomer, 100 umol (85 mg)
D5160 FAM 亚磷酰胺,6-异构体 FAM Phosphoramidite, 6-Isomer, 250 mg
F5160 FAM 亚磷酰胺,6-异构体 FAM Phosphoramidite, 6-Isomer, 1 g
G5160 FAM 亚磷酰胺,6-异构体 FAM Phosphoramidite, 6-Isomer, 5 g
24260 Long trebler 亚磷酰胺 Long trebler phosphoramidite, 250 mg
34260 Long trebler 亚磷酰胺 Long trebler phosphoramidite, 500 mg
44260 Long trebler 亚磷酰胺 Long trebler phosphoramidite, 1 g
11460 芘 亚磷酰胺 dU Pyrene phosphoramidite dU, 100 mg
21460 芘 亚磷酰胺 dU Pyrene phosphoramidite dU, 250 mg
41460 芘 亚磷酰胺 dU Pyrene phosphoramidite dU, 1 g

* 上述中文名称仅供参考,订货以货号和英文名称为准

支持CPG固相修饰 CPG solid supports
支持CPG固相修饰 CPG solid supports
炔烃CPG修饰剂 500 Alkyne CPG modifier 500

Solid support for the synthesis of 3′-modified oligonucleotides with alkyne group for Click Chemistry.

Alkyne CPG modifier 500
三氟乙酸-氨基修饰剂 CPG 500 TFA-Amino modifier CPG 500

TFA-amino modifier CPG for oligo synthesis. 500 ?, high load controlled pore glass.

TFA-Amino modifier CPG 500

产品列表

货号
中文名
产品描述
22860 炔烃CPG修饰剂 500 Alkyne CPG modifier 500, 250mg
42860 炔烃CPG修饰剂 500 Alkyne CPG modifier 500, 1g
52860 炔烃CPG修饰剂 500 Alkyne CPG modifier 500, 5g
21860 三氟乙酸-氨基修饰剂 CPG 500 TFA-Amino modifier CPG 500, 250 mg
41860 三氟乙酸-氨基修饰剂 CPG 500 TFA-Amino modifier CPG 500, 1 g
51860 三氟乙酸-氨基修饰剂 CPG 500 TFA-Amino modifier CPG 500, 5 g

* 上述中文名称仅供参考,订货以货号和英文名称为准

三磷酸酯修饰 Modified triphosphates
三磷酸酯修饰 Modified triphosphates
氨基-11-ddUTP Amino-11-ddUTP

Amino-11-ddUTP is a triphosphate for the modification of DNA.

Amino-11-ddUTP
氨基-11-dUTP Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

TFA-Amino modifier CPG 500

产品列表

货号
中文名
产品描述
15040 氨基-11-ddUTP Amino-11-ddUTP, 1 umol
25040 氨基-11-ddUTP Amino-11-ddUTP, 5 umol
45040 氨基-11-ddUTP Amino-11-ddUTP, 25 umol
16040 氨基-11-dUTP Amino-11-dUTP, 1 umol
26040 氨基-11-dUTP Amino-11-dUTP, 5 umol
46040 氨基-11-dUTP Amino-11-dUTP, 25 umol

* 上述中文名称仅供参考,订货以货号和英文名称为准

Lumiprobe蛋白质组学试剂 Proteomic reagents

Lumiprobe蛋白质组学试剂 Proteomic reagents

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,上海金畔生物Lumiprobe官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线,所有规格产品保证货期5-7工作日到货,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规(市场上部分是分装,质量不能保证),质量保证,价格极具竞争力,而且我们的货期仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe蛋白质组学试剂 Proteomic reagents

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling

Hide pricing

Cat. #QuantityPriceLead timeBuy this product

1A041 5 nmol
2A041 10 nmol
3A041 25 nmol

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.

Hide pricing

Cat. #QuantityPriceLead timeBuy this product

1B041 5 nmol
2B041 10 nmol
3B041 25 nmol

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.

Hide pricing

Cat. #QuantityPriceLead timeBuy this product

1C041 5 nmol
2C041 10 nmol
3C041 25 nmol

产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
2A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 10 nmol
3A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 25 nmol
1B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 5 nmol
2B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 10 nmol
3B041 Cy3-N-羟基琥珀酰亚胺酯 Cyanine3 NHS ester minimal dye, 25 nmol
1C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 5 nmol
2C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 10 nmol
3C041 Cy5-N-羟基琥珀酰亚胺酯 Cyanine5 NHS ester minimal dye, 25 nmol

* 上述中文名称仅供参考,订货以货号和英文名称为准

辅助试剂 Auxiliary reagents
抗坏血酸 Ascorbic acid

ascorbic acid for reduction of Cu(II)-TBTA complex.

Ascorbic acid
铜-TBTA络合物,10mM溶于55%的二甲亚砜水溶液
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

DMF (dimethylformamide)
标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.


产品列表

货号
中文名
产品描述
1A041 Cy2-N-羟基琥珀酰亚胺酯 Cyanine2 NHS ester minimal dye, 5 nmol
12050 抗坏血酸 Ascorbic acid, 10 mg
21050 铜-TBTA络合物,10mM溶于55%的二甲亚砜水溶液 Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO, 1.5 mL
13050 标记级别二甲基酰胺 DMF (dimethylformamide), labeling grade, 1 mL

* 上述中文名称仅供参考,订货以货号和英文名称为准

PCR/qPCR PCR/qPCR
罗丹明对照品染料用于快速PCR ROX reference dye for qPCR

ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.

SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x

SYBR Green I stock solution for Real Time PCR, 100x.

SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x

SYBR Green I dye for staining dsDNA in gelen.


产品列表

货号
中文名
产品描述
31110 罗丹明对照品染料用于快速PCR ROX reference dye for qPCR, 500 uL
41010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1 mL
51010 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 1.5 mL
51010-5 SYBR Green I 染料用于实时PCR SYBR Green I for Real Time PCR, 100x, 5×1.5 mL
20010 SYBR Green I 凝胶染色溶液 SYBR Green I Gel Staining Solution, 10000x, 1 mL