亲和配体包覆聚苯乙烯 Affinity Ligand Coated Polystyrene

亲和配体包覆聚苯乙烯 Affinity Ligand Coated Polystyrene

Affinity Ligand Coated Polystyrene 亲和配体包覆微球

When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the spheres with the appropriate ligand. Binding proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of Ab/Ag complexes in immunoprecipitation.

当环境要求迅速发展的微球试剂,粒子包覆亲和结合蛋白提供了一个现成的解决方案。亲和结合协议是直截了当的,通常涉及球与适当配体的简单孵育。结合蛋白还提供了一个最佳的定位配体的机会,例如生物素化寡核苷酸在链霉亲和素包被的微球上的末端固定,或通过蛋白A或蛋白G对抗体的fc特异性固定。

包被结合蛋白的颗粒在纯化中也有重要的用途。链霉亲和素包被微球常用于生物素化PCR扩增产物的分离.蛋白A和蛋白G微球用于纯化抗体和Fc标记融合蛋白,以及免疫沉淀中抗体/Ag复合物的回收。

STREPTAVIDIN  链霉亲和素

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, ProActive® Microspheres. Streptavidin coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

我们的链亲和素和超亲和素™包被微球提供简单和非常稳定的结合生物素化分子,如抗体,肽和寡核苷酸,用于诊断,筛选和亲和纯化。看见Technote 101,未雨绸缪®微球。链霉亲和素包被微球可作为1%固体(w/v)的水悬浮液,分4种标准体积:1ml、2mL、5ml或10 ml。

STREPTAVIDIN POLYSTYRENE  链霉亲和素聚苯乙烯

Catalog Number Nominal Diameter Specification Range Product Data Sheet
CP01000 0.10µm 0.090 – 0.110µm PDS 721.pdf
CP01001 0.20µm 0.190 – 0.210µm PDS 721.pdf
CP01003 0.50µm 0.470 – 0.530µm PDS 721.pdf
CP01004 1.00µm 0.95 – 1.05µm PDS 721.pdf
CP01005 3.0µm 2.80 – 3.20µm PDS 721.pdf
CP01006 5.00µm 4.80 – 5.20µm PDS 721.pdf
CP01007 10.00µm 9.50 – 10.50µm PDS 721.pdf
CP01008 15.0µm 14.50 – 15.50µm PDS 721.pdf

REFERENCES

Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, & Russell SJ. (2016) Development of a microsphere-based system to facilitate real-time insulin monitoring. Journal of diabetes science and technology, 10(3), 689-696.(10µm SA microspheres coated with biotin-anti-insulin Ab for insulin monitoring in a microdevice)

Streptavidin magnetic polymer 

The surfaces of Encapsulated magnetic microspheres are expected to be free of iron oxide, while the Classical version will have some exposed iron oxide. COMPEL microspheres (UMC catalog codes) are highly uniform and spherical. Fluorescent magnetic microspheres are available as ~1% solids (w/v) aqueous suspensions in these standard volumes: 1mL, 5mL, 10mL, and 100mL. See spectra for available fluorescent dyes.

链霉亲和素磁性聚合物
包覆磁性微球的表面不含氧化铁,而经典的磁性微球表面会有一些暴露的氧化铁。强制微球(UMC编录码)具有高度的均匀性和球形。荧光磁性微球可作为1%固体(w/v)的水悬浮液在这些标准体积:1ml,5ml,10毫升和100毫升。见可用光谱荧光染料.

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CME0101 SA / Encapsulated Magnetic Polymer 0.86µm 0.7 – 1.2µm PDS 721.pdf
UMC0100 SA/ COMPEL 3µm 2.50 – 3.50µm PDS 721.pdf
UMC0101 SA / COMPEL 6µm 5.50 – 6.50µm PDS 721.pdf
UMC0102 SA / COMPEL 8µm 7.50 – 8.50µm PDS 721.pdf

STREPTAVIDIN FLUORESCENT POLYSTYRENE

Catalog Number Fluorescence Color Nominal Diameter Specification Range Product Data Sheet
CFDG001 SA / Dragon Green 0.20µm 0.190 – 0.210µm PDS 721.pdf
CFDG002 SA / Dragon Green 0.40µm 0.370 – 0.430µm PDS 721.pdf
CFFR002 SA / Flash Red 0.40µm 0.370 – 0.430µm PDS 721.pdf
CFDG003 SA / Dragon Green 0.50µm 0.470 – 0.530µm PDS 721.pdf
CFDG004 SA / Dragon Green 1.00µm 0.95 – 1.05µm PDS 721.pdf
CFFR004 SA / Flash Red 1.00µm 0.95 – 1.05µm PDS 721.pdf

STREPTAVIDIN DYED POLYSTYRENE

Streptavidin-coated dyed microspheres supplied as ~1% solids (w/v) aqueous suspensions.

链霉亲和素染色聚苯乙烯
链霉亲和素涂层染色微球提供~1%固体(w/v)的水悬浮液。

Catalog Number Coating / Dye Nominal Diameter Specification Range
CDCR001 SA / Crimson Red 0.20µm 0.190 – 0.210µm
CDCB001 SA / Cabo Blue 0.20µm 0.190 – 0.210µm

REFERENCES

Sepp, A, Tawfik, D, Griffiths, D(2002) Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. FEBS Letters V532(3)P455–458

Kahanovitz L, Seker E, Marks RS, Yarmush ML, Konry T, Russell SJ (2016) Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring. J Diabetes Sci Technol 10(3):689-96

Tanga MYH, Shum HC (2016) One-step immunoassay of C-reactive protein using droplet microfluidics Lab Chip, 16, 4359-4365

BIOTIN

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. Biotin coated beads are supplied as ~1% solids (w/v) aqueous suspensions. See TechNote 101, ProActive® Microspheres, for sample applications and procedures.

生物素
我们的生物素包覆的微球在结合游离亲和素的能力方面已经完全被表征,因此在确定正确的配体浓度时,需要进行最小的优化。生物素包覆微珠是以~1%固体(w/v)的水悬浮形式提供的。看见Technote 101,未雨绸缪®用于样品应用和程序的微球。

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CP10000 Polymeric / Biotin 10µm 9.50 – 10.50µm PDS 724.pdf

PROTEIN A AND PROTEIN G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, ProActive® Microspheres. Protein A and Protein G coated microspheres are available as ~1% solids (w/v) aqueous suspensions in four standard volumes: 1mL, 2mL, 5mL, or 10mL.

蛋白A和蛋白G
蛋白A和蛋白G是很容易与多种抗体的FC区结合的蛋白质。因此,它们被广泛用于血清或细胞培养上清液中抗体的亲和纯化,以及免疫沉淀蛋白复合物的捕获。当蛋白A和蛋白G微球作为试剂进行试验和检测时,固定化抗体可以交联到蛋白A或蛋白G包被载体上,以保持长期稳定性。看见Technote 101,未雨绸缪®微球。蛋白A和蛋白G包覆的微球可作为1%固体(w/v)的水悬浮液,分4种标准体积:1ml、2mL、5ml或10 ml。

Catalog Number Description Nominal Diameter Specification Range Product Data Sheet
CP02000 Polymer / Protein A 1.00µm 0.95-1.05µm PDS 722.pdf
CP02001 Polymer / Protein A 5.00µm 4.80-5.20µm PDS 722.pdf
CP02002 Polymer / Protein A 10.0µm 9.50-10.50µm PDS 722.pdf
PMG3N ProMag™ (Magnetic) / Protein G 3µm 2.70 – 3.30µm PDS 722.pdf