Harlan TD.07108 Zn Defic. Diet (0.5) 锌缺乏饲料使用说明书

Harlan TD.07108 Zn Defic. Diet (0.5) 锌缺乏饲料使用说明书

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet
TD.07108 Zn Defic. Diet (0.5)

Formula g/Kg
Egg White Solids, spray-dried 200
Dextrose, monohydrate 634.2376
Corn Oil 100
Cellulose 29.986
Mineral Mix, Zn Deficient (81264) 25.6902
Chromium Potassium Sulfate, dodecahydrate 0.02
Zinc Sulfate, heptahydrate 0.0022
Vitamin Mix, Teklad (40060) 10
Biotin 0.004
Ethoxyquin, antioxidant 0.02
Purple Food Color 0.04

Footnote

A zinc deficient diet with 0.5 ppm added zinc as zinc sulfate and purple food coloring. Total zinc estimated to be 2 – 3.5 ppm (background + added zinc). Modified from TD.85419. For the series TD.07108-TD.07110.

Selected Nutrient Information1

% by weight % kcal from
Protein 16.1 16.6
Carbohydrate 58.3 60.1
Fat 10.0 23.2
Kcal/g 3.9
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Adjusted Zinc
  • Color Coded
  • Zinc Sulfate

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Harlan TD.160118 High Fat Atherogenic Western Diet with 1% Added Choline

Harlan TD.160118 High Fat Atherogenic Western Diet with 1% Added Choline

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Formula g/Kg
Casein 195
DL-Methionine 3
Sucrose 341.46
Corn Starch 138.25
Anhydrous Milkfat 210
Cholesterol 1.5
Cellulose 50
Mineral Mix, AIN-76 (170915) 35
Calcium Carbonate 4
Vitamin Mix, Teklad (40060) 10
Ethoxyquin, antioxidant 0.04
Choline Chloride 11.75

Footnote

Modification of the popular Western diet to add 1% choline from choline chloride.

Selected Nutrient Information

% by weight % kcal from
Protein 17.3 15.4
Carbohydrate 47.4 42.2
Fat 21.2 42.4
Kcal/g 4.5
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Western Diet
  • Atherogenic & Obesogenic
  • Added Choline

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (crumbly)
  • Minimum order 3 Kg
  • Irradiation available upon request

Options (fees will apply)

  • Rush order (pending availability)
  • Irradiation (see Product Specific Information)
  • Vacuum packaging (1 and 2 Kg)

Harlan TD.00158 维生素A缺乏饮食 Vitamin A Deficient Diet 使用说明

Harlan TD.00158 维生素A缺乏饮食 Vitamin A Deficient Diet

货号:TD.00158

英文名称:Vitamin A Deficient Diet

Formula g/Kg
Casein,”iamin-Freet”Test 200
L-Cystine 3
Com Starch 397.486
Maltodextrin 132
Sucrose 109.755
Soybean Oil 70
Cellulose 50
Mineral Mix, AIN-93G-MX (94046) 35
Choline Bitartrate 2.5
TBHQ, antioxidant 0.014
Niacin 0.03
Calcium Pantothenate 0.016
Pyridoxine HCI 0.007
Thiamin (81%) 0.006
Riboflavin 0.006
Folic Acid 0.002
Biotin 0.0002
Vitamin B.2 (0.1% in mannitol) 0.025
Vitamin E DL-alpha tocooheryl acetate (500 IU/g) 0.15
Vitamin D3 cholecalciferol (500,000 lU/g) 0.002
Vitamin K1. phyloquinone 0.0008

Footnote
This modification of diet AIN-93G (TD 94045) omits vitamin A palmitate and
substitutes alcohol-extracted casein for regular casein. Diet should contain
less than 20 IU vitamin AVkg diet.

Selected Nutrient Information

% by weight % kcal from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcalg 3.8

Key Features
+ Purified Diet
+ Vitamin A
+ VFT Casein
+ AIN-93G Modification

Key Planning Information
+ Products are made fresh to order
+ Store product at 4°C or lower   将产品存放在4°C或更低的温度下

+Use within 6 months (applicable to most diets)
+ Box labeled with product name,
manufacturing date, and lot number
+ Replace diet at minimum once per week
More frequent replacement may be advised

+ Lead time:
2 weeks non-irradiated
4 weeks irradiated

Product Specific Information
1/2”Peilet of Powder (free flowing)  1/2”粉饼(自由流动)
+ Minimum order 3 Kg
+ Irradiation not advisea
Contact a nutntionist for recommendations

Harlan Teklad 动物饲料 Vitamin A Diet(25,000)成分表

Harlan Teklad 动物饲料 Vitamin A Diet(25,000)成分表

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula g/Kg
Casein, “Vitamin-Free”Test 200
L-Cystine 3
Com Starch 397.486
Maltodextrin 132
Sucrose 109.705
Soybean Oil 70
Cellulose 50
Mineral Mix, AIN-93G-MX (94046) 35
Choline Bitartrate 2.5
TBHQ, antioxidant 0.014
Niacin 0.03
Calcium Pantothenate 0.016
Pyridoxine HCI 0.007
Thiamin (81%) 0.006
Riboflavin 0.006
Folic Acid 0.002
Biotin 0.0002
Vitamin B12 (0.1% in manitol) 0.025
Vitamin E DL alpha tocopheryl acetate (500 IU/g) 0.15
Vitamin D3. cholecalciferol (500,000 IU/g) 0.002
Vitamin K1, phylloquinone 0.0008
Vitamin A Acetate (500,000 IU/g) 0.05

Foothote

This modification of diet AIN-93G (TD.94045) replaces vitamin A palmitate with
tamin A acetate and increases the total concentration to approximately 25,000
/kg. Alcohol-extracted casein is substituted for regular casein.

Selected Nutrient Information

% by weight % kcal from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcal/g 3.8

Values are calculated from ingredient analysis or manufacturer data

Key Features

+ Purifield   Diet
+Vitamin A
+AlN 93G Modification
+ Rodent

Key Planning Information

+ Product are made Fresh fo order
+ store product at 4° C or IoWer
+ Use within 6 months(appIicable to most diets)
+ Box Labeled with product name
manufaGturing date,and Iot number
+ Replace diet at nninimunη once per weekation

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 成分表

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

啮齿动物饲料(2018,625强力霉素)

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.375
Doxycycline Hyclate 0.625

Footnote   Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用说明

This diet was designed to deliver a daily dose of 2-3 mg of doxycycline based on consumption of 4-5 g/d by a mouse. Doxycycline hyclate contains approximately 87% doxycycline.

这种饮食被设计为以小鼠每天4-5 g / d的消耗量提供2-3 mg强力霉素的日剂量。 盐酸多西环素含有约87%的多西环素。

Selected Nutrient Information

  % by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Doxycycline
  • Gene Induction
  • 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

日本TOSOH 分析色谱柱-TOSOH代理商

日本TOSOH 分析色谱柱-TOSOH代理商

日本东曹株式会社(TOSOH CORPORATION)始建于1935年,原名东洋曹达工业株式会社,现已发展成为涉足石油化工、基础化学、精细化工、电子材料、生命科学等多项事业的全球性跨国企业,2009年度全球销售额已超过80亿美金。
主要业务集中在化工原料、精细化工产品、电子材料及色谱层析产品的销售上。

Size-exclusion chromatography (SEC)
SEC separates molecules based on their size, or more
precisely, their hydrodynamic volume. It is a well known
technique for the separation of macromolecules like natural
and synthetic polymers. When an aqueous eluent is used,
SEC is also referred to as gel filtration chromatography
(GFC). When an organic eluent is used, SEC is referred to as
gel permeation chromatography (GPC). GPC is the method
of choice for the characterization of polymer molecular
weight and molecular weight distribution.
Tosoh Bioscience is a leading manufacturer of SEC/GPC
columns and developed GPC instruments for the Asian
market for more than 35 years. This brochure gives an
overview over the Tosoh Bioscience GPC/SEC product
portfolio for the separation of water and organic soluble
polymers. The separation of proteins and biopolymers with
silica based TSKgel SEC columns is not covered here.

Gel Permeation Chromatography (GPC)
GPC plays an important role in the characterization of
organic-soluble polymers in the chemical and petrochemical
industries. TSKgel GPC columns contain particles prepared
from polystyrene crosslinked with divinylbenzene. Available
products are grouped according to their relative lack of
adsorptive properties and the speed of analysis.

货号 品名及描述 产品分类
5102 G2000SW 7.5*600 Column
5103 G3000SW 7.5*600 Column
5104 G4000SW 7.5*600 Column
5105 G2000PW 7.5*600 Column
5106 G3000PW 7.5*600 Column
5107 G4000PW 7.5*600 Column
5108 G5000PW 7.5*600 Column
5109 G6000PW 7.5*600 Column
5146 G2000SW 21.5*600 Column
5147 G3000SW 21.5*600 Column
5148 G4000SW 21.5*600 Column
5202 STD PSA-300(11587) Std. Polymer
5203 STD PS A-500(11589) Std. Polymer
5204 STD PS A-1000(11591) Std. Polymer
5205 STD PS A-2500(11593) Std. Polymer
5206 STD PS A-5000(11595) Std. Polymer
5207 STD PS F-1(11597) Std. Polymer
5208 STD PS F-2(11599) Std. Polymer
5209 STD PS F-4(11601) Std. Polymer
5210 STD PS F-10(11603) Std. Polymer
5211 STD PS F-20 Std. Polymer
5212 STD PS F-40(11607) Std. Polymer
5213 STD PS F-80 Std. Polymer
5214 STD PS F-128(11611) Std. Polymer
5215 STD PS F-288(11613) Std. Polymer
5216 STD PS F-380 Std. Polymer
5217 STD PS F-450 Std. Polymer
5218 STD PS F-550 Std. Polymer
5219 STD PS F-700 Std. Polymer
5220 STD PS F-850 Std. Polymer
5221 STD PS F-2000 Std. Polymer
5371 Guard SW 7.5*75 Column
5748 ENDFITTING3/81/1610F” parts
5758 Guard SW 21.5*75 Column
5760 G1000PW 7.5*300 Column
5761 G2000PW 7.5*300 Column
5762 G3000PW 7.5*300 Column
5763 G4000PW 7.5*300 Column
5764 G5000PW 7.5*300 Column
5765 G6000PW 7.5*300 Column
5773 STD O SE-KIT Std. Polymer
5788 G2000SW 7.5*300 Column
5789 G3000SW 7.5*300 Column
5790 G4000SW 7.5*300 Column
6172 ODS-120A 21.5*300 Column
6191 ENDFITTING1RU1/1610F” parts
6211 STD O SE-2(11573) Std. Polymer
6212 STD O SE-5(11575) Std. Polymer
6213 STD O SE-8(11577) Std. Polymer
6214 STD O SE-15(11579) Std. Polymer
6215 STD O SE-30(11581) Std. Polymer
6216 STD O SE-70(11583) Std. Polymer
6217 STD O SE-150(11585) Std. Polymer
6476 STD PS Oligomer Kit Std. Polymer
6477 STD PS High Polymer Kit Std. Polymer
6727 G2000SW 21.5*300 Column
6728 G3000SW 21.5*300 Column
6729 G4000SW 21.5*300 Column
6758 Guard PWH 21.5*75 Column
6762 Guard PWH 7.5*75 Column
6763 Guard PWL 7.5*75 Column
6819 TOPOFF SW, 1mL Bulk gel
6837 IC Anion-PW, 4.6*50  Column
6839 IC-Anion-SW 4.6*50 Column
7113 Guard HXL 6.0*40 Column
7124 ODS-120A 4.6*250 Column
7125 ODS-120T 4.6*250 Column
7127 NH2-60 4.6*250 Column
7128 OH-120, 4.6*250m  Column
7129 ODS-120A 7.8*300 Column
7130 ODS-120T 7.8*300 Column
7134 ODS-120T 21.5*300 Column
7147 Silica-60, 4.6*250  Column
7156 SCX 6.0*150 Column
7157 SAX 6.0*150 Column
7158 SCX 7.8*300 Column
7161 SP-5PW 7.5*75 Column
7162 CM-3SW 7.5*75 Column
7163 DEAE-3SW 7.5*75 Column
7164 DEAE-5PW 7.5*75 Column
7165 SP-2SW 4.6*250 Column
7166 QAE-2SW 4.6*250 Column
7167 CM-2SW 4.6*250 Column
7168 DEAE-2SW 4.6*250 Column
7190 TMS-250 4.6*75 Column
7210 Guard DEAE-5PW Kit Column
7211 Guardkit SP-5PW Column
7447 HW-40S 500mL Toyoarl
7448 HW-40F 500mL Toyoarl
7449 HW-40C 500mL Toyoarl
7450 HW-40EC 500mL Toyoarl
7451 HW-40S 250mL Toyoarl
7452 HW-50S 500mL Toyoarl
7453 HW-50F 500mL Toyoarl
7455 HW-50S 250mL Toyoarl
7456 HW-55S 500mL Toyoarl

Harlan AIN diet formulas

Harlan AIN diet formulas

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

These formulas are purified diets with commonly used refined ingredients. These basic formulas are often modified for a specific research purpose, such as altering the fat source, a vitamin or mineral level, or adding a compound. See the FAQ section to learn more about the history of these formulas, AIN-76A, AIN-93G, and AIN-93M.

  • CA.170481 AIN-76A purified diet
  • TD.94045 AIN-93G purified diet
  • TD.94048 AIN-93M purified diet

These formulas (as well as other purified diets) do not contain alfalfa and can be used to reduce background autofluorescence in certain imaging applications. Our Global Rodent Diets are also alfalfa-free and are suitable for imaging work. Please contact us for further information about these formulas or modifications.

Other related product codes:

  • TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased)
  • TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased)
  • TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased)
  • TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil.

Harlan AIN diet formulas

这些配方是含有常用精制成分的纯正饮食。这些基本配方通常是为特定的研究目的而修改的,例如改变脂肪来源、维生素或矿物质水平,或添加一种化合物。见常见问题了解更多关于这些公式的历史的章节,AIN-76A,AIN-93g,和AIN-93m。

  • CA.170481AIN-76A纯饲料
  • TD.94045AIN-93g纯饲料
  • TD.9404893米纯饲料

这些配方(以及其他纯化饲料)不含苜蓿,可用于减少背景自荧光在某些成像应用中。我们的全球啮齿动物饮食也是无苜蓿的,适合成像工作.有关这些公式或修改的进一步信息,请与我们联系。

其他相关产品代码:

  • TD.94096适合辐照的AIN-76A版本(维生素含量增加)
  • TD.97184适合辐照的an-93g版本(维生素含量增加)
  • TD.00102AIN-93m适合辐照的版本(维生素含量增加)
  • TD.95092用玉米油代替大豆油的n-93g改性。

Harlan Teklad Diet ingredients

Harlan Teklad Diet ingredients

Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Diet ingredients

You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.

Our ingredients

We offer several ingredients for individual sale so that you can develop your own blends.

“VFT” casein

  • “Vitamin-free” test casein (alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
  • The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

  • The common vitamin mixes shown below use sucrose or corn starch as a carrier
  • Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
  • Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
  • For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
  • Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
  • Minimum order is 500 g for both stock and custom vitamin mixes
  • Our available vitamin mixes are commonly used in diets, and are available from stock
  • Vitamin mixes are shipped within a few days of order

Our Formula examples

  • CA.40060 Teklad vitamin mix
  • CA.40077 AIN-76A vitamin mix
  • TD.94047 AIN-93 vitamin mix

Mineral mixes

  • The common mineral mixes shown below as examples use sucrose as a carrier
  • Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
  • Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
  • For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
  • Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
  • These mineral mixes are commonly used in diets, and are available from stock
  • Our mineral mixes are shipped within a few days of order

Formula examples:

  • CA.170915 AIN-76 mineral mix
  • TD.94046 AIN-93G mineral mix
  • TD.94049 AIN-93M mineral mix

Other mixes (may or may not be available from stock):

  • TD.83171 Vitamin mix without A, D, E, choline
  • TD.81062 Iron deficient mineral mix based on AIN-76A
  • TD.79055 Calcium and phosphorus deficient mineral mixed based on AIN-76
  • TD.98057 Calcium and phosphorus deficient mineral mixed based on AIN-93
  • CA.170760 Rogers-Harper mineral mix

你可能想要在你的研究地点或设施准备你自己的饮食,如果是这样的话,你取决于成分是最高的质量。Envigo可以为您提供许多相同的成分,用于生产我们先进的特克拉德定制研究饮食,以便您可以开发自己的配方。和往常一样,我们的营养专家在这里帮助您为您的配料和最终配方做出最佳决定。

我们的配料

我们提供几个配料供个人销售,以便您可以开发自己的混合。

“VFT”酪蛋白

  • “无维生素”测试酪蛋白(酒精提取)最适合于那些需要精确控制脂肪或维生素含量的纯正试验饮食配方。
  • VFT酪蛋白中许多维生素含量的降低使得它成为许多维生素研究的首选蛋白质来源。因此,“无维生素”的绰号是在许多年前给提取出来的酪蛋白起的。

维生素混合物

  • 以下常见的维生素混合物使用蔗糖或玉米淀粉作为载体。
  • 定制的维生素混合物可能使用纤维素,如果非营养载体是必要的。
  • 维生素混合物的制定考虑到了饮食中的包容率。多用或少用会损害你的实验动物的健康。
  • 对于那些自己准备饮食的人来说,维生素预混剂是向饮食中添加维生素的一种有效方法。
  • 维生素混合物应储存在4摄氏度或更低的接收。如果使用时间超过1个月,建议使用摄氏-20度。
  • 库存和定制维生素混合物的最低订购量为500克。
  • 我们可用的维生素混合物通常用于饮食,并可从库存中获得。
  • 维生素混合物在订购后几天内发货。

我们的公式示例

  • CA.40060 特克拉德维生素混合物
  • CA.40077 AIN-76A维生素混合物
  • TD.94047 AIN-93维生素混合物

矿物混合物

  • 下面所示的常见矿物混合物是以蔗糖为载体的。
  • 如果需要非营养的载体,定制的矿物混合物可以使用纤维素,也可以不使用载体。
  • 我们的矿物质混合物是根据饮食包容率来制定的。多用或少用会损害动物的健康。
  • 对于那些自己准备饮食的人来说,矿物预混料是向饮食中添加矿物质的有效方法。
  • 矿物混合物应存放在干燥、凉爽的环境中,并在收到后一年内使用。库存和自定义矿物混合物的最低订购量为500克。
  • 这些矿物混合物通常用于饮食,并可从库存中获得。
  • 我们的矿物混合物在订货后几天内装运。

公式示例:

  • CA.170915 AIN-76矿物混合物
  • TD.94046 AIN-93g矿物混合物
  • TD.94049 AIN-9300万矿物混合物

其他混合材料(可能从库存中获得,也可能无法从库存中获得):

  • TD.83171不含A,D,E,胆碱的维生素混合物
  • TD.81062基于AIN-76A的缺铁矿物配料
  • TD.79055 AIN-76混合钙磷缺乏矿物
  • TD.98057AIN-93混合钙磷缺乏矿物
  • CA.170760 罗杰斯-哈珀矿物混合物

日本关东化学Kanto 07183-33 溴化铯 Caesium bromide CAS 7787-69-1

日本关东化学Kanto 07183-33 溴化铯 Caesium bromide CAS 7787-69-1

日本关东化学代理商–上海金畔生物

代理优势:货期2-3周。

欢迎访问关东化学株式会社Kanto官网或者咨询我们获取更多产品信息。

Product No. 07183-33
Product name Cesium bromide, 4N
Grade high purity reagent
Purity >99.99%(Im.S.)
Package 25g

溴化铯 Caesium bromide

CasNo:7787-69-1  MDL:MFCD00010954

分子式:Br Cs

分子量:212.81

Lumiprobe 抗体标记试剂盒说明书Antibody labeling kits

Lumiprobe 抗体标记试剂盒说明书Antibody labeling kits

The kit is used for the preparation of labeled antibodies having 2.5-3 labels per antibody. The kit contains all reagents and materials for ten labeling reactions, each for 100 ug of the antibody. The dye is an NHS ester, taken in controlled excess. It reacts with the amine groups of lysines the antibody at mild alkaline pH. The purification of the antibody is achieved by gel filtration using spin columns. These kits contain sulfonated, water soluble dyes, that are most suitable for the labeling of sensitive proteins, including antibodies.

Kit components

Kit component Count
1321-10rxn
10 reactions
3321-10rxn
10 reactions
7321-10rxn
10 reactions
5321-10rxn
10 reactions
6321-10rxn
10 reactions
N1320, Sulfo-Cyanine3 NHS ester, 1 rxn 10
N3320, Sulfo-Cyanine5 NHS ester, 1 rxn 10
N7320, Sulfo-Cyanine5.5 NHS ester, 1 rxn 10
N5320, Sulfo-Cyanine7 NHS ester, 1 rxn 10
N6320, Sulfo-Cyanine7.5 NHS ester, 1 rxn 10
Desalting spin column, PBS 10 10 10 10 10
Desalting receptacle vial, 1.5 mL 10 10 10 10 10
Desalting waste vial, 2 mL 10 10 10 10 10
PBS tablet, for 100 mL of buffer 1 1 1 1 1
15050, DMSO, labeling grade, 1 mL 1 1 1 1 1
1584-05mL, Sodium azide solution, 3%, 0.5 mL 1 1 1 1 1
1689-15mL, Sodium bicarbonate, 126 mg 1 1 1 1 1

Store at temperature between +3 °C and +20 °C. Do not freeze!

Shelf life 12 months

Protocol

1. Antibody preparation.

Optimal conditions for the labeling reaction are the following: antibody at a concentration of 1 mg/mL dissolved in 0.1 M sodium hydrocarbonate solution. Sodium azide is compatible with the labeling. If concentration of antibody is below 1 mg/mL, it should be concentrated. Antibody preparation should be free of aminoacids, proteins like BSA and buffer components that bring pH to unfavourable (2-7.5 or 9-12) ranges.

2. Setting up the reaction.

2.1. Add a solution of antibody in sodium hydrocarbonate (100 ug in 100 uL*) to the fluorophore, vortex, and incubate for 30 min at room temperature.

* if a smaller amount of antibody is to be labeled, dissolve lyophilized dye in 10 uL of anhydrous DMSO, and take 1 uL of the solution per 10 ug of antibody. The solution of dye in DMSO should be consumed immediately.

3. Purification of the labeled antibody.

3.1. Prepare the column. The column should be at room temperature prior to use. The gel should be resuspended using vortex. Put the column into a waste receptacle (without cap), and centrifuge for 2 min at 1,000 g (speed must be carefully controlled – for standard 6 cm rotor, 1,000 g = 3800 rpm). The small bulge of the column should be oriented outwards.

3.2. Apply 400 uL of 1x PBS buffer, centrifuge for 2 min at 1,000 g.

3.3. Remove the column from waste receptacle. Put it into collection tube (with cap). Apply 100 uL of the reaction mixture onto the column, incubate for 1 min, and elute the antibody by centrifugation for 2 min at 1,000 g. Add 1/100 volume of 100x sodium azide. Antibodies can be aliquoted to improve their shelf life. Current aliquot can be stored at +4°С the rest at -20°С.

4. Measurement of dye to antibody ratio.

To calculate dye to antibody ratio, measure absorption spectrum of the conjugate, or absorption at 280 nm (AAB), and at dye absorption maximum (ADye). A typical absorption spectrum of a dye labeled antibody is shown below. Depending on the dye, wavelength of the maximum may vary.

Labeled antibody absorption spectrum

An absorption spectrum of a labeled antibody contains dye peak (with longer wavelength), and antibody peak (at around 280 nm). Dye to antibody ratio is calculated using the following formula:

Calculation of dye to antibody ratio

with Dye/AB – average number of fluorophores per antibody molecule, Adye – optical density of the sample at dye absorption maximum, AAB – sample optical density at 280 nm, εAB – molar extinction coefficient of antibody at 280 nm (210,000 for IgG), εDye – molar extinction coefficient of dye at absorption maximum (see table below), CF280 – correction factor for dye (see table below).

Dye λmax, nm ε CF280
sulfo-Cyanine3 548 162,000 0.06
sulfo-Cyanine5 646 271,000 0.04
sulfo-Cyanine5.5 673 195,000 0.11
sulfo-Cyanine7 750 240,600 0.04
sulfo-Cyanine7.5 778 222,000 0.09

Example of calculation

After labeling of IgG antibody with sulfo-Cyanine5 and purification, a solution has been obtained with absorption spectrum above. Determine dye to protein ratio.

Using absorption spectrum, the following values can be found: ADye = 0.184 at dye absorption maximum (646 nm, see the table), AAB = 0.066 (at 280 nm), εAB = 210000 for IgG; εDye = 271,000, and CF280 = 0.04.

Dye/AB – is 2.43 fluorophore molecules per antibody.

Interpretation of results, and antibody storage

In most cases, optimal dye to antibody ratio is 2-3 dye molecules per antibody. Increase of dye loading above this value does not improve fluorescent signal because of the concentrational quenching of the fluorescence. When too few fluorophores are attached during the reaction, decrease antibody loading per reaction. Use of expired kit can also lead to this result.

We recommend to test binding of labeled antibodies with their antigens. The labeled antibodies can be stored at -20°С. An aliquot that is currently in use, should be stored at +4°С to avoid freeze-thaw cycles. The stability of the conjugate is determined by antibody itself rather than fluorophore or the chemical bond. Labeled antibodies should not be exposed to direct sunlight for extended time, but tolerate ambient lighting well.

Related kits

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.

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sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.

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sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.

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sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.

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sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.

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