日本JaICA老化制御研究所代理

日本JaICA老化制御研究所代理

上海金畔生物代理日本JaICA全线产品,欢迎新老客户访问日本JaICA官网或者咨询我们获取更多相关产品信息。

日本JaICA代理

日本老化制御研究所代理

日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。
本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。

产品类型:
1.         DNA氧化检测产品;
2.         蛋白氧化检测产品;
3.         脂肪氧化检测产品;
4.         抗氧化检测产品。

特性:
1.         高专一性、高灵敏性;
2.         不与RNA损伤产生交叉反应;
3.         易于操作、简单快速;
4.         无需昂贵的设备和样品预处理。

The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.

Type of Products:

  1. DNA oxidation;
  2. Protein oxidation;
  3. Lipid oxidation;
  4. Antioxidant assay.

Features:

l          High specificity & High sensitivity;

l          No cross-reactivity with RNA damage;

l          Easy operation & Speedy;

l          There is no need for expensive equipment and sample pretreatment.

日本老化制御研究所

产品名称 货号 规格
New 8-OHdG Check KOG200SE 96 wells
New8-OhdGCheck Trial Package KOG-200TE 32 wells
Hexanoyl-Lys (HEL) ELISA KHL-700E 96 wells
Anti 8-OHdGmonoclonal antibody MOG-020P 20 micro gram of IgG
MOG-100P 100 micro gram of IgG
Anti thymidineglycol(TG) monoclonal antibody MTG-100P 100 micro gram of IgG
Hexanoyl-Lys (HEL) ELISA KHL-700E 96 wells
Anti HELmonoclonal antibody(clone 5F12) MHL-021P 20 ug of IgG
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20 ug of IgG
Anti 4-HNEmonoclobal antibody MHN-020P 20 micro gram of IgG/ vial
MHN-100P 100 micro gram of IgG/ vial
Anti Acroleinmonoclonal antibody MAR-020n 20 micro gram of IgG
MAR-100n 100 micro gram of IgG
Anti Malondialdehyde (MDA)monoclonal antibody MMD-030n 30 micro gram
Anti 4-HHEmonoclonal antibody MHH-030n 30 micro gram
Anti Crotonaldehyde(CRA) monoclonal antibody MCA-030n 30 micro gram
Anti Methylglyoxal(MG) monoclonal antibody MMG-030n 30 micro gram
Anti 7-ketocholesterol(7-KC) monoclonal antibody MKC-020n 20 micro gram
MKC-100n 100 micro gram
Anti dibromotyrosine monoclonal antibody MBY-020P 20 micro gram IgG
Anti dityrosine(DT) monoclonal antibody MDT-020P 20 micro gram
Test kit forPotential Anti Oxidant (PAO) KPA-050 96 wells

日本老化JaICA中国代理及JaICA品牌介绍

日本老化JaICA中国代理及JaICA品牌介绍

Japan Institute for the Control of Aging (JaICA)

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

日本老化制御研究所 Jaica代理

日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。

本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。

产品类型:

1. DNA氧化检测产品;

2. 蛋白氧化检测产品;

3. 脂肪氧化检测产品;

4. 抗氧化检测产品。

特性:

1. 高专一性、高灵敏性;

2. 不与RNA损伤产生交叉反应;

3. 易于操作、简单快速;

4. 无需昂贵的设备和样品预处理。

日本Jaica

The Japan Institute for the Control of Aging has conducted research into control of aging since its foundation under the doctrine of contributing health promotion. As a result, the institute succeeded in developing a DNA oxidative damage measuring kit, thus taking the lead for this field.
The institute now sells many products related to oxidative stress, and such products are utilized in the fields of medicine, pharmacy, biochemistry, food sciences, etc. The institute also performs assay services utilizing its unique technologies. Preventive medicine and anti-aging are receiving increased attention with the advancement of aging society. In these areas, our Institute will contribute to realizing a healthy, long-lived society by developing and supplying research products.

独特的氧化应激标记物

日本控制衰老研究所从事衰老研究,目的是促进人类健康。该研究所成功研发了检测DNA氧化损伤的试剂盒,并且在这个领域处于领先地位。该研究所目前销售很多关于氧化应激研究方面的产品。这些产品在医学,药学,生物化学和食品方面都有运用。该研究所也提供定制服务。随着步入老龄化社会,预防医学和抗衰老收到越来越多的关注。

JaICA从事试剂盒销售和定制服务。试剂盒部分,研究所研发和生产用于检测氧化应激标记物的试剂盒。单抗和ELISA试剂盒可用于检测活性氧产生的氧化损伤产物。定制服务部分,使用本研究所研发的产品和积累的经验检测尿液和血液样品,检测食品。近来预防医学、抗衰老和功能性食品的关注越来越多,因此全球医院,大学,研究所、食品企业和制药企业分析氧化应激标记物,衰老相关激素和矿物质需求也越来越多。

◆氧化应激标记物概况

类型 产品名称 运用 备注
尿液 血清 组织
DNA 氧化 8-OHdG   Check ELISA Kit 是(动物) 普遍使用的氧化应激标记物
检测时间:大概3.5小时
8-OHdG   Check ELISA Kit (High Sensitivity) 是(动物) 人血清、组织和培养细胞的更低检测限。
anti-8-Hydroxy-2′-deoxyguanisine   [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反应性
anti-Thymidine   Glycol [TG], mAb (2E8) DNA特异性氧化应激标记物
脂类氧化 Hexanoyl-Lys   [HEL] ELISA Kit n:6 多不饱和脂肪酸早期氧化的新标记物
anti-Hexanoyl-Lys   [HEL], mAb (5F12) 免疫组化,免疫印迹和ELISA
anti-Hydroxy-2-nonenal   [4-HNE], mAb(HNEJ-2) 普遍使用的脂类氧化标志物
anti-Acrolein   [ACR], mAb (5F6) 免疫组化, 免疫印迹 和 ELISA.
anti-Malondialdehyde   [MDA], mAb (1F83) 免疫组化 和 免疫印迹
anti-4-Hydroxy-2-hexenal   [4-HHE], mAb (HHE53) 免疫组化
anti-Crotonaldehyde   [CRA], mAb (82D3) 免疫组化
anti-Methylglyoxal   [MG], mAb (3C) 免疫组化
anti-7-Ketocholesterol   [7-KC], mAb (35A) 胆固醇氧化标记物
蛋白氧化 Dityrosine   [DT] ELISA Kit 蛋白氧化新型标记物。检测时间:过夜 + 1.5小时。
anti-Dityrosine   [DT], mAb (1C3) 免疫组化, 免疫印迹 和 ELISA.
anti-Dibromo-tyrosine   [DiBrY], mAb (3A5) 炎症相关的蛋白氧化标记物
抗氧化检测 Total   Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水总抗氧化检测,能够疏水和亲水的抗氧化剂。

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本JaICA Test kit for Potential Anti Oxidant (PAO)

日本老化制御研究所 Jaica代理

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

Suitable for detection of total antioxidant capacity in serum and food extracts. For research use only.

Antioxidant assay:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.

Principle of this assay:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu++ to Cu+), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.

Specifications:

Method: Colormetric assay(detection: 480 – 492 nm)
Assay range: 21.9 – 4378 碌mol/L (cupric ion reducing power)
Format: 96 wells
Storage: Room temperature (10 – 25掳C)
Applications: Human and animal serum samples, foods and beverage samples.
Required but not provided: A micro plate reader (measuring wavelength 492 nm)
Pipettes and pipette chips
Plastic test tubes
Distilled water
NaOH, HCl solution and pH meter (Not required if standards are prepared with distilled water only).

 

Content of this kit: 试剂盒组成

Standard (Uric acid powder): 1 vial
Sample diluent: 1 bottle
Cu++ solution: 1 bottle
Stop solution: 1 bottle
Micro titer plate: 1 plate (96 wells)

Assay procedure:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 碌L of Sample Diluent, and add 10 碌L of standards or diluted samples.
3) Pour 200 碌L of mixture to Micro titer plate. Use 200 碌L of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 碌L of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 碌L of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (碌mol/L).
1mM of uric acid = 2189 碌mol/L (copper reducing power)

References

1) Oxidative imbalance and cathepsin D changes as peripheral blood biomarkers of Alzheimer disease: A pilot study
E Strafacea, P Matarresea, L Gambardella, R Vona, A Sgadari,MC Silveri, W Malorni
FEBS Letters 579, p2759-766 (2005)
2) Oxidative stress and its association with coronary artery disease and different atherogenic risk factors
C. VASSALLE, L. PETROZZI , N. BOTTO, M. G. ANDREASSI and G. C. ZUCCHELLI
Journal of Internal Medicine 256, p308-315(2004)
3) Antioxidant capacity as a reliable marker of stress in dairy calves transported by road
P Pregel, E Bollo, FT Cannizzo, B Biolatti, E Contato, and PG Biolatti
Veterinary Record 156, p53-54 (2005)
4) Vitamin E-coated dialyzers reduce oxidative stress related proteins and markers in hemodialysis ? a molecular biological approach.
LA Calo, A Naso, E Pagnin, PA Davis, M Castoro, R Corradin, P Riegler, C Cascone, W Huber and A Piccoli
Clinical Nephrology, Vol.62(5), p355-361 (2004)
5) Oxidative stress-related factors in Bartter’s and Gitelman9s syndrome: relevance for angiotensin IIsignalling.
Calo LA, Pagnin E, Davis PA, Sartori M, Semplicini A.
Nephrol Dial Transplant 18(8) p1518-1525 (2003)
6) Effect of epoetin on HO-1 mRNA level and plasma antioxidants in hemodialysis patients.
Calo LA, Stanic L, Davis PA, Pagnin E, Munaretto G, Fusaro M, Landini S, Semplicini A, Piccoli A.
Int. J Clin. Ther 41(5), p187-192 (2003)
7) Restored Antioxidant Capacity Parallels the Immunologic and Virologic Improvement in Children with Perinatal Human Immunodeficiency Virus Infection Receiving Highly Active Antiretroviral Therapy.
M Martino, F Chiarelli, M Moriondo, M Torello, C Azzari, and L Galli
Clinical Immunology 100(1),p82-86 (2001)
Product name Code Assay range Assay time Format
Test kit for Potential Anti Oxidant (PAO) KPA-050 21.9-4378 碌mol/L 5 minutes 96 wells

日本JaICA Test kit for Potential Anti Oxidant (PAO)   说明书  技术资料

Instruction manual Technical information

日本Japan Institute for the Control of Aging (JaICA)

日本Japan Institute for the Control of Aging (JaICA)

日本老化制御研究所 Jaica代理

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

独特的氧化应激标记物

日本控制衰老研究所从事衰老研究,目的是促进人类健康。该研究所成功研发了检测DNA氧化损伤的试剂盒,并且在这个领域处于领先地位。该研究所目前销售很多关于氧化应激研究方面的产品。这些产品在医学,药学,生物化学和食品方面都有运用。该研究所也提供定制服务。随着步入老龄化社会,预防医学和抗衰老收到越来越多的关注。

JaICA从事试剂盒销售和定制服务。试剂盒部分,研究所研发和生产用于检测氧化应激标记物的试剂盒。单抗和ELISA试剂盒可用于检测活性氧产生的氧化损伤产物。定制服务部分,使用本研究所研发的产品和积累的经验检测尿液和血液样品,检测食品。近来预防医学、抗衰老和功能性食品的关注越来越多,因此全球医院,大学,研究所、食品企业和制药企业分析氧化应激标记物,衰老相关激素和矿物质需求也越来越多。

◆氧化应激标记物概况

 

类型 产品名称 运用 备注
  尿液 血清 组织  
DNA 氧化 8-OHdG   Check ELISA Kit 是(动物) 普遍使用的氧化应激标记物
检测时间:大概3.5小时
8-OHdG   Check ELISA Kit (High Sensitivity) 是(动物) 人血清、组织和培养细胞的更低检测限。
anti-8-Hydroxy-2′-deoxyguanisine   [8-OHdG],mAb(N45.1) 8-OHGua/8-OHG低交叉反应性
anti-Thymidine   Glycol [TG], mAb (2E8) DNA特异性氧化应激标记物
脂类氧化 Hexanoyl-Lys   [HEL] ELISA Kit n:6 多不饱和脂肪酸早期氧化的新标记物
anti-Hexanoyl-Lys   [HEL], mAb (5F12) 免疫组化,免疫印迹和ELISA
anti-Hydroxy-2-nonenal   [4-HNE], mAb(HNEJ-2) 普遍使用的脂类氧化标志物
anti-Acrolein   [ACR], mAb (5F6) 免疫组化, 免疫印迹 和 ELISA.
anti-Malondialdehyde   [MDA], mAb (1F83) 免疫组化 和 免疫印迹
anti-4-Hydroxy-2-hexenal   [4-HHE], mAb (HHE53) 免疫组化
anti-Crotonaldehyde   [CRA], mAb (82D3) 免疫组化
anti-Methylglyoxal   [MG], mAb (3C) 免疫组化
anti-7-Ketocholesterol   [7-KC], mAb (35A) 胆固醇氧化标记物
蛋白氧化 Dityrosine   [DT] ELISA Kit 蛋白氧化新型标记物。检测时间:过夜 + 1.5小时。
anti-Dityrosine   [DT], mAb (1C3) 免疫组化, 免疫印迹 和 ELISA.
anti-Dibromo-tyrosine   [DiBrY], mAb (3A5) 炎症相关的蛋白氧化标记物
抗氧化检测 Total   Antioxidant Capacity [PAO] Test Kit Food
Samples
血清、食物和酒水总抗氧化检测,能够疏水和亲水的抗氧化剂。

 

日本JaICA试剂盒产品目录

日本JaICA试剂盒产品目录

日本老化制御研究所 Jaica代理

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

序号 详细名称 品牌 规格
KOG-200SE New 8-OHdG Check ELISA kit/8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) JaICA 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit/高灵敏 8羟基脱氧茑苷ELISA 试剂盒 JaICA 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody/8羟基脱氧茑苷 单克隆抗体 JaICA 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody/4羟基壬烯酸单抗 JaICA 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody/二溴络氨酸单抗 JaICA 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody/抗胸苷乙二醇单克隆抗体 JaICA 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody/二络氨酸抗体 JaICA 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12)/乙酰赖氨酸单克隆抗 JaICA 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit/乙酰赖氨酸加合物ELISA试剂盒 JaICA 96 wells
KPA-050E Test kit for Potential Anti Oxidant (PAO)/总抗氧化能力ELISA试剂盒 JaICA 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody/丙二醛单克隆 JaICA 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody/炳烯醛单克隆 JaICA 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody/4羟基 2烯醛单抗 JaICA 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody/抗巴斗醛单抗 JaICA 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody/甲基乙二醛单抗 JaICA 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 20 micro gram
MKC-100n Anti 7-ketocholesterol (7-KC) monoclonal antibody/7酮基胆固醇单抗 JaICA 100 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.)/血清预处理超滤装置(MW切断10kDa,Pall公司) JaICA 1 unit
KDT-010E Dityrosine (DT) ELISA/双络氨酸ELISA试剂盒 JaICA  96 wells

 

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

日本老化制御研究所自成立以来就对抗衰老进行研究,它是在有助于健康促进的理念下创建的。结果,此研究所在开发DNA氧化损伤测试试剂盒方面取得了很大的成功,并在该领域处于了领先地位。

本研究所现在销售许多氧化应激相关产品,这些产品可应用于医学,药学,生化学,食品科学等。它还利用其独特的技术开展检测服务。随着社会老龄化的发展,预防医学与抗衰老引起了越来越多的关注,在这些领域中,我们研究所将会通过开发和提供研究产品来助于营造一个健康,长寿的社会。

产品类型:

DNA氧化检测产品;

蛋白氧化检测产品;

脂肪氧化检测产品;

抗氧化检测产品。

特性:

高专一性、高灵敏性;

不与RNA损伤产生交叉反应;

易于操作、简单快速;

无需昂贵的设备和样品预处理。

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

日本老化制御研究所 Jaica代理

日本 JaICA代理 Anti Di Tyrosine (DT) 单抗

产品名称: Anti Di Tyrosine (DT) 单抗

英文名称: Anti Di Tyrosine (DT) monoclonal antibody

产品货号: MDT-020P

产品规格: 20ug

产品产地: 日本

产品商标: JaICA

 

Code Product Name  
KOG-200SE New 8-OHdG Check ELISA kit 96 wells
KOG-200TE New 8-OHdG Check ELISA Trial Package (32wells) 32 wells
KOG-HS10E Highly Sensitive 8-OHdG Check ELISA kit 96 wells
MOG-100P Anti 8-OHdG monoclonal antibody 100 micro gram
MOG-020P Anti 8-OHdG monoclonal antibody 20 micro gram
MHN-100P Anti 4-HNE monoclonal antibody 100 micro gram
MHN-020P Anti 4-HNE monoclonal antibody 20 micro gram
MBY-020P Anti Di bromo tyrosine (DiBrY) monoclonal antibody 20 micro gram
MTG-100P Anti Tymidine Glycol (TG) monoclonal antibody 100 micro gram
MDT-020P Anti Di Tyrosine (DT) monoclonal antibody 20 micro gram
MHL-021P Anti Hexanoyl-Lys(HEL) monoclonal antibody (clone 5F12) 20 micro gram
KHL-700E Hexanoyl-Lys(HEL) ELISA kit 96 wells
KPA-050 Test kit for Potential Anti Oxidant (PAO) 96 wells
MMD-030n Anti malondialdehyde (MDA) monoclonal antibody 30 micro gram
MAR-020n Anti acrolein (ACR) monoclonal antibody 20 micro gram
MAR-100n Anti acrolein (ACR) monoclonal antibody 100 micro gram
MHH-030n Anti 4-hydroxy-2-hexenal (4-HHE) monoclonal antibody 30 micro gram
MCA-030n Anti crotonaldehyde (CRA) monoclonal antibody 30 micro gram
MMG-030n Anti methyglyoxal (MG) monoclonal antibody 30 micro gram
MKC-020n Anti 7-ketocholesterol (7-KC) monoclonal antibody 20 micro gram
OPF-010E NEW Ultra filter unit for serum pretreatment (MW cut off 10kDa, Pall Corp.) 1 unit
KDT-010E Dityrosine (DT) ELISA  96 wells
OPNUCP1 Nuclease P1 from Penicillium citrinum 500 units

 

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA代理 Dityrosine (DT) ELISA

日本 JaICA 老化 Antibody系列产品

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

日本老化制御研究所 Jaica代理

Dityrosine (DT) ELISA kit.

Suitable for urine samples. For research use only.

A novel biomarker for protein oxidation

Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.

Recently, dityrosine is reported to exist also in urine samples. It is expected that DT may be a novel protein oxdation marker, which is non-invasively detectable. DT ELISA kit is designed for quantitative measurement of DT especially in urine samples.

试剂盒组成:

Samples: Urine
Specifity: Specific to dityrosine (tyrosine dimer)
Assay range: 0.05 – 12 碌mol/L
Measurement: 450 nm
Quantity: 96 wells
Assay time: Overnight and 2 hours
Storage: 2 – 8 掳C. Don’t freeze.
Expiration: Printed on a package

参考文献:

1) Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T:
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000).
2) Hattori Y, Mukaide T, Jiang L, Kotani T, Tsuda H, Mano Y, Sumigama S, Hirayama T, Nagasawa H,Kikkawa F, Toyokuni S:
Catalytic ferrous iron in amniotic fluid as a predictive marker of human maternal-fetal disorders.
J Clin Biochem Nutr 56(1),p57-63(2015) doi: 10.3164/jcbn.14-82
Product name Code Assay range Format Application
Dityrosine (DT) ELISA KDT-010E 0.05 – 12 碌mol/L 96 wells Urine

使用说明书:

Instruction manual (PDF) Technical information

相关产品:

Related products: Anti DT monoclonal antibody

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit

Hexanoyl-lysine adduct (HEL) ELISA kit

Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.

已酰-赖氨酸(HEL)ELISA试剂盒,适用于尿液,血清和细胞培养样品氧化应激的评估。
仅供研究使用。

什么是己酰-赖氨酸?

Biomarker for early stage of lipid oxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.

由活性氧(ROS)引起的脂质氧化损伤在一些疾病、细胞功能损害和衰老中起重要作用。醛类如丙二醛(MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一。但最近在脂质过氧化的早期阶段,发现13-氢过氧十八烷酸(13-HPODE)与蛋白质共价结合。己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基。HEL是通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)的氧化修饰形成。HEL可能是脂质过氧化初始阶段有用的生物标志物。现在我们已经开发了HEL单克隆抗体和ELISA试剂盒,并且可以在氧化修饰的低密度脂蛋白(LDL)、人动脉粥样硬化病变、人尿液和血清中检测HEL。另据报道,大鼠肌肉在运动期间形成HEL,并且通过抗氧化剂(如类黄酮)是禁止形成的。

己酰-赖氨酸(HEL)ELISA试剂盒

JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.

JaICA公司与Toshihiko Osawa博士(名古屋大学)和Yoji Kato博士(兵库大学)合作开发了Hexanoyl-Lys(HEL)ELISA试剂盒。
该ELISA试剂盒可应用于人和动物的尿液、血清和细胞培养。

Assay principle: Competitive ELISA (detection: 450 nm)
Specifity: Specific to N-epsilon-Hexanoyl-Lysine adduct
Measuring range: 2 – 700 nmol/L
Time to answer: Over night and 2 hours.
Format: 96 wells (54 samples in single assay)
Applications: Urine, serum and cultured cells from human and animals.
Storage: Store at 2 – 8掳C (don’t freeze).
Expiry: 2 years after the day of manufacturing.
Required but not provided: 50 碌L micropipettor and pipette tips
8-channel (50-200 碌L) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 掳C incubator
Microtiter plate reader (measuring wavelength 450 nm)

技术参数
检测原理:竞争法ELISA (检测: 450 nm处);
特异性:特异于N-epsilon-己酰-赖氨酸加合物;
测定范围:2 – 700 nmol/L;
反应速度:过夜+2小时;
规格:96孔(54 samples in single assay);
应用:人和动物的尿液、血清和细胞培养物;
储存:2-8℃(避免冷冻);
保质期:2年。

Content of this kit

HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets

试剂盒组成:

HEL-包被微孔板: 1 块板(96孔)
一抗 (即用型): 1 瓶
二抗: 1 瓶
二抗缓冲液: 1 瓶
底物溶液 (TMBZ溶液): 1 瓶
底物缓冲液: 1 瓶
洗涤液 (5X): 1 瓶
终止液: 1 瓶
标准液 (6 标准): 各1 瓶
封板膜: 2 片

Product name Code Assay range Application
Hexanoyl-Lys (HEL) ELISA KHL-700E 2-700 nmol/L Urine, serum, cultured cells and other biological materials

参考文献:

1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.

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  • 9(±)-羟基十八碳二烯酸ELISA试剂盒 NWLSS™ 9(±)-hydroxyoctadecadienoic ac
  • 己酰赖氨酸ELISA试剂盒 NWLSS™ Hexanoyl Lysine ELISA Kit
  • 丙二醛检测试剂盒 NWLSS™ Malondialdehyde(MDA) Assay Kit
  • 抗4-羟基壬烯醛单克隆抗体 Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNE
  • 抗4-羟基壬烯醛单克隆抗体 Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNE

使用手册        质量控制        技术资料

Instruction manual Quality control Technical information

日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA代理 Anti HEL monoclonal antibody

日本 JaICA 老化 Antibody系列产品

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

日本老化制御研究所 Jaica代理

抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)

抗己酰-赖氨酸单克隆抗体 Anti Hexanoyl-Lysine(HEL) monoclonal antibody(5F12)
适用于免疫组织化学和ELISA。

Anti hexanoyl-Lysine adduct monoclonal antibody.

Suitable for immunohistocheistry and ELISA. For research use only.

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

由活性氧(ROS)引起的脂质氧化损伤在一些疾病,细胞功能损害和衰老中起重要作用。醛类(如丙二醛/MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一,但最近在脂质过氧化的早期阶段,发现13-hydroperoxyoctadecanoic acid(13-HPODE)与蛋白质1)共价结合。
己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基, HEL通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)氧化修饰形成。
抗己酰-赖氨酸(HEL)单克隆抗体对于含有HEL的蛋白质和肽是特异的,适用于通过免疫组织化学和免疫印迹的HEL检测。

A new biomarker for lipid peroxidation   脂质过氧化新的生物标志物

Cisplatin-treated rat kidney. 顺铂处理的大鼠肾脏
(Dr. Sugiyama, Tottori Univ.)  (Sugiyama博士)

Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)

HEL在人类动脉粥样硬化病变的检测  (Naito博士)

Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 碌g/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 碌g/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20C

• 技术参数
克隆#:5F12;
亚基:IgG1 kappa;
抗原:N epsilon己酰-钥孔血蓝蛋白(HEL-KLH);
特异性:特异于己酰-赖氨酸加合物;
形式:冻干粉,100μg/mL(200μL),PBS(pH7.4),包含5%蔗糖,1% BSA;
应用:免疫组织化学(多聚甲醛固定的组织推荐抗体浓度为2μg/mL)和ELISA;
储存:低于-20°C

参考文献:

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

产品信息:

Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20ug of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20ug of IgG Immunohistochemistry Discontinued

使用说明书
免疫组织化学方法
技术信息
克隆#5F12和5H4的比较

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

日本 JaICA老化Anti 4-HNE monoclonal antibody (HNEJ-2)

日本 JaICA 老化 Antibody系列产品

上海金畔生物作为日本 JaICA 老化中国代理商,欢迎新老客户访问日本 JaICA 老化官网或者咨询我们获取更多日本 JaICA 老化产品线价格说明书等信息。

日本老化制御研究所 Jaica代理

抗4-羟基壬烯醛单克隆抗体

Anti 4-hydroxy-2-nonenal(4-HNE) monoclonal antibody(HNEJ-2)
货号:MHN-100P
品牌:JaICA
规格: 100µg

产品说明 / Product Overview:

Anti 4-HNE monoclonal antibody (HNEJ-2)

Structure of 4-hydroxy-2-nonenal

 

脂质氧化标志物

适用于免疫组织化学和免疫印迹.(仅供研究使用)

关于4-羟基壬烯醛(4-hydroxy-2-nonenal; 4-HNE)

活性氧(ROS)参与活体内的脂质过氧化,膜脂质是ROS的主要靶标之一。 ROS在过氧化过程中形成各种醛。 4-羟基-2-壬烯醛(4-HNE)是一种α,β不饱和醛,可以通过omega-6不饱和脂肪酸(例如亚油酸和花生四烯酸)的过氧化形成。 特别需要指出的是,据报道在活体内形成的4-HNE源自磷脂结合的花生四烯酸。 4-HNE可能是脂质过氧化最可靠的生物标志物之一。

大肠癌细胞的高倍视图:腺癌(箭头所示)和非肿瘤性上皮细胞(star x240)。
(S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

技术参数

克隆号#:HNEJ-2;
免疫原:4-HNE修饰的钥孔血蓝蛋白(KLH);
亚基:小鼠IgG1(kappa);制备为腹水,并纯化硫酸铵;
性状:冻干粉(含20μg或100μg IgG);
特异性:该抗体对于用其它醛(如2-壬烯醛,2-己烯醛,1-己醛,4-羟基-2-己烯醛,甲醛或戊二醛)处理的蛋白质显示出几乎可以忽略的反应性;
应用:免疫组织化学和免疫印迹;
储存:-20°C以下5年;运输时,室温下至少可稳定保持7天。

Clone #: HNEJ-2
Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin
Subclass: Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified.
Form: Lyophilized Powder (containing 20 碌g or 100 碌g of IgG)
Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
Applications: Immunohistochemistry and western blotting.
Storage: Less than -20掳C for 5 years. When transport, stable at least 7 days at room temperature.

References

Immunohistochemistry:
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997)
Western blotting:
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995)
Product name Code Content
Anti 4-HNE monoclobal antibody MHN-020P 20 ug of IgG/ vial
MHN-100P 100 ug of IgG/ vial

使用说明书
免疫组织化学操作方法
技术资料