Harlan Teklad TD.08485 Low Fat Control Diet低脂饲料

Harlan Teklad TD.08485 Low Fat Control Diet低脂饲料

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Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat

Formula g/Kg
Casein 195
DL-Methionine 3
Sucrose 120
Corn Starch 432.99
Maltodextrin 100
Anhydrous Milkfat 37.2
Soybean Oil 12.8
Cellulose 50
Mineral Mix, AIN-76 (170915) 35
Calcium Carbonate 4
Vitamin Mix, Teklad (40060) 10
Ethoxyquin, antioxidant 0.01


This is modified from TD.88137 to reduce fat, reduce sucrose, and remove cholesterol.

elected Nutrient Information1


  % by weight % kcal from
Protein 17.3 19.1
Carbohydrate 61.3 67.9
Fat 5.2 13.0
Kcal/g 3.6  
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Modification of TD.88137
  • Low Fat & No Added Cholesterol
  • Reduced Sucrose

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated


Harlan TD.96355 Ketogenic Diet 生酮饲料说明书

Harlan TD.96355 Ketogenic Diet 生酮饲料说明书

Harlan Teklad动物饲料

Harlan is a leading provider of essential, pre-clinical and non-clinical contract research, research models, lab animal diets, and services. Our focus is on providing customers with products and services to optimize the discovery and safety of new medicines and compounds.

Formula g/Kg
Casein 173.3
DL-Methionine 2.6
Vegetable Shortening, hydrogenated (Crisco) 586.4
Corn Oil 86.2
Cellulose 87.97
Vitamin Mix, Teklad (40060) 13
Choline Bitartrate 2.5
TBHQ, antioxidant 0.13
Mineral Mix, Ca-P Deficient (79055) 20
Calcium Phosphate, dibasic 19.3
Calcium Carbonate 8.2
Magnesium Oxide 0.4


A very high fat diet with almost no carbohydrate. The ratio of fat to protein + carbohydrate in this ketogenic diet is approximately 4.25.

Selected Nutrient Information1

  % by weight % kcal from
Protein 15.3 9.2
Carbohydrate 0.5 0.3
Fat 67.4 90.5
Kcal/g 6.7  
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Ketogenic
  • Crisco
  • Rodent

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated


CA.170481 AIN-76A Purified Diet AIN-76A 纯化饲料 50kg harlan
TD.94045 AIN-93G Purified Diet AIN-93G 纯化饲料 50kg harlan
TD.94048 AIN-93M Purified Diet AIN-93M 纯化饲料 50kg harlan
TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased) 适合辐照的AIN-76A 饲料 维生素含量增加 50kg harlan
TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased) 适合辐照的AIN-93G 饲料 维生素含量增加 50kg harlan
TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased) 适合辐照的AIN-93M 饲料 维生素含量增加 50kg harlan
TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil. AIN-93G饲料 豆油改为玉米油 50kg harlan
TD.06414 Adjusted Calories Diet (60/Fat) 卡路里调控饲料 60%脂肪 50kg harlan
TD.06415 Adjusted Calories Diet (45/Fat) 卡路里调控饲料 45%脂肪 50kg harlan
TD.03584 35% Lard Diet (Adj., No C) 35%猪油饲料 50kg harlan
TD.93075 Adjusted Calories Diet (55/fat) 卡路里调控饲料 55%脂肪 50kg harlan
TD.95217 Adjusted Fat Diet 脂肪控饲料 50kg harlan
TD.96132 Adjusted Fat Diet 脂肪控饲料 50kg harlan
TD.88137 Adjusted Kcal Diet (42% from fat 大卡调控饲料 42%脂肪 50kg harlan
TD.08811 45% Kcal Fat Diet (21% MF, 2% SBO) 45%大卡脂肪饲料 42%脂肪 50kg harlan
TD.06416 Adjusted Calories Diet (10/Fat) (a possible control for TD.06415 & TD.06414 listed above) 卡路里调控饲料 10%脂肪 50kg harlan
TD.08810 Low Glycemic Control Diet 低血糖饲料 50kg harlan
TD.96355 Ketogenic Diet 生酮饲料 50kg harlan
TD.84224 Essential Fatty Acid Deficient Diet 必须氨基酸缺损饲料 50kg harlan
TD.88137 Adjusted Calories Diet (42% from fat) 卡路里调控饲料 42%脂肪 50kg harlan
TD.88051 Cocoa Butter and Purina Chow Diet 椰子油和Purina Chow饲料 50kg harlan
TD.90221 Cocoa Butter Diet with 75% Purina Mouse (5015) 50kg harlan
TD.94059 5015, Cocoa Butter, Chol., etc. (excludes cholate) 50kg harlan
TD.02028 Atherogenic Rodent Diet (1.25% Chol. & 0.5% Cholic Acid) 50kg harlan
TD.01383 2018 + 2% Cholesterol 50kg harlan
TD.86143 Vitamin A Deficient Diet 50kg harlan
TD.89123 Vitamin D Deficient Diet 50kg harlan
TD.88163 Vitamin E Deficient Diet 50kg harlan
TD.95247 Folic Acid Deficient Diet 50kg harlan
TD.80396 Iron Deficient Diet 50kg harlan
TD.85419 Zinc Deficient Diet 50kg harlan
TD.88239 Potassium Deficient Diet 50kg harlan
TD.95027 Low Calcium Diet (0.4% P) 50kg harlan
TD.96348 Diet (20% Lactose, 2% Ca, 1.25% P) 50kg harlan
TD.92163 Selenium Deficient Diet 50kg harlan
TD.95125 Iodine Deficient (0.15% PTU) 50kg harlan
TD.93328 Protein Free Diet 50kg harlan
TD.90016 6% Protein Diet 50kg harlan
TD.91352 20% Protein Diet 50kg harlan
TD.90018 40% Protein Diet 50kg harlan
TD.89247 60% Fructose Diet 50kg harlan
TD.86489 Diet with Adjusted Sucrose/Cornstarch 50kg harlan
TD.96348 Diet (20% Lactose, 2% Ca, 1.25% P) 50kg harlan
TD.98090 70% Carbohydrate Diet 50kg harlan
TD.96355 Ketogenic Diet (almost no carbohydrate) 50kg harlan
TD.99366 Amino Acid Diet 50kg harlan
TD.90262 Methionine & Choline Deficient Diet 50kg harlan
TD.00434 Folic Acid Deficient Diet 50kg harlan
TD.99386 No Lysine AA Diet 50kg harlan
TD.90228 Sodium Deficient Diet 50kg harlan
TD.96208 0.49% NaCl Diet 50kg harlan
TD.90229 1% NaCl Diet 50kg harlan
TD.92034 4% NaCl Diet 50kg harlan
TD.92012 8% NaCl Diet 50kg harlan
TD.01306 Rodent Diet (2018 + 625 doxycycline) 50kg harlan
TD.98186 Rodent Diet (7012 + 200 doxycycline) 50kg harlan
TD.92033 Swine Diet (15% Lard, 1.2% Cholesterol) 50kg harlan
TD.92052 Swine Diet (15% Lard, 1.5% Cholesterol) 50kg harlan
TD.93296 Swine Diet (15% Lard, 2% Cholesterol) 50kg harlan
CA.40060 Teklad Vitamin Mix 50kg harlan
CA.40077 AIN-76A Vitamin Mix 50kg harlan
TD.94047 AIN-93 Vitamin Mix 50kg harlan
CA.170915 AIN-76 Mineral Mix 50kg harlan
TD.94046 AIN-93G Mineral Mix 50kg harlan
TD.94049 AIN-93M Mineral Mix 50kg harlan
TD.79055 Calcium & Phosphorus Deficient Mineral Mixed based on AIN-76 50kg harlan
TD.98057 Calcium & Phosphorus Deficient Mineral Mixed based on AIN-93 50kg harlan


Harlan TD.95027 低钙饲料 Low Calcium Diet (0.4% P)

Harlan TD.95027 低钙饲料 Low Calcium Diet (0.4% P)

Harlan Teklad动物饲料

Harlan TD.95027 低钙饲料 Low Calcium Diet (0.4% P)

品牌:Harlan Teklad Custom Diet
英文名称:Low Calcium Diet (0.4% P)

Formula g/Kg
Casein 200.0
L-Cystine 3.0
Sucrose 342.188
Corn Starch 320.0
Soybean Oil 60.0
Cellulose 40.0
Mineral Mix, Ca-P Deficient (79055) 13.37
Potassium Phosphate, monobasic 11.43
Vitamin Mix, Teklad (40060) 10.0
Ethoxyquin, antioxidant 0.012


Casein contributes approximately 0.01% calcium or less. Diet contains about 0.4% phosphorus and 2200 IU vitamin D/kg diet. TD.97191 is a possible ingredient matched control with ~0.6% calcium. For questions about this or other calcium adjusted diets

Selected Nutrient Information1

  % by weight % kcal from
Protein 17.7 18.6
Carbohydrate 63.4 66.7
Fat 6.2 14.7
Kcal/g 3.8  

Values are calculated from ingredient analysis or manufacturer data

Key Features

Purified Diet



Harlan Teklad LM-485 Mouse/Rat Sterilizable Diet

Harlan Teklad LM-485 Mouse/Rat Sterilizable Diet

Harlan Teklad动物饲料

Home +Teklad diet, bedding and enrichment +Teklad laboratory animal diets +Standard natural ingredient diets +Rodent diets +Traditional rodent diets

Traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. While traditional diets will supply the known nutrient needs of your laboratory animals, we recommend you consider the use of a diet from our newer Teklad Global Rodent Diet® line for your modern research needs.

传统的啮齿动物饮食是几十年前根据对啮齿动物营养,成分和当时饮食制造的了解而制定的。 尽管传统饮食可以满足实验动物的已知营养需求,但我们建议您考虑使用我们较新的Teklad Global RodentDiet®系列饮食来满足您的现代研究需求。


Teklad 4% fat mouse/rat diet 7001 
Teklad S-2335 mouse breeder sterilizable diet 7004  7904 
Teklad LM-485 mouse/rat sterilizable diet 7012  7912 
NIH-31 Modified Open Formula Mouse/Rat sterilizable diet 7013  7913 
NIH-31 Open formula mouse/rat sterilizable diet 7017  7917 
NIH-07 Mouse/rat diet 7022  7922 
Teklad low salt mouse/rat sterilizable diet 7034 
Teklad rodent diet 8904 , 8604 , 8728C 
Teklad 22/5 rodent diet 8640 
Teklad sterilizable rodent diet 8656  7956 

Harlan Teklad 2018 global 18% protein rodent diets

Harlan Teklad 2018  global 18% protein rodent diets

Harlan Teklad动物饲料


Home + Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Standard natural ingredient diets + Rodent diets + 2018 Teklad global 18% protein rodent diets

Product Features

  • Designed to support gestation, lactation, and growth
  • Formulated to reduce soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
  • Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from 150 to 250 mg/kg
  • Exclusion of alfalfa reduces chlorophyll, greatly improving fluorescent optical imagingclarity
  • Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)
Teklad rodent diets Non-autoclavable form Autoclavable Irradiated
Teklad global 18% protein 2018, 2018C 2018S, 2018SX 2918
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.


  • 用于支持妊娠、哺乳和生长的
  • 减少豆粕的配方,从而尽量减少异黄酮的存在。异黄酮是大豆的主要类型。植物雌激素在实验室动物饮食中发现的
  • 典型的异黄酮浓度(大豆苷元+染料木素苷元当量)为150至250毫克/千克。
  • 紫花苜蓿的排斥降低了叶绿素含量,大大提高了叶绿素含量。荧光光学成像清晰性
  • 缺乏动物蛋白质和鱼粉可使亚硝胺(一种潜在的致癌物质)的存在减少到最低限度。
特克勒德啮齿类食物 不可蒸压式 高压釜 辐照
Teklead全局18%蛋白 2018,2008 c 2008 S, 2018SX 2918

Harlan Teklad 诱导动物NAFLD and NASH饲料

Harlan Teklad 诱导动物NAFLD and NASH饲料

Teklad diet, bedding and enrichment    Teklad laboratory animal diets   Custom research diets   NAFLD and NASH


  • 长期喂饲可诱发肥胖、代谢综合症及轻微纳什或
  • 短时间喂饲可诱发严重纳什的肝脏特征而不引起肥胖或胰岛素抵抗的饮食。


Dietary methods to induce NAFLD/NASH in rodents can be split into two common categories:

  • diets fed for longer periods of time to induce obesity, metabolic syndrome, and mild NASH or
  • diets fed for short periods of time to induce hepatic features of severe NASH without inducing obesity or insulin resistance

This page provides further information on dietary methods to induce NAFLD/NASH. We’ve also prepared a downloadable NASH/NAFLD mini paper.

The tables below highlight diet options from both of the above categories. For more complete descriptions of NAFLD/NASH models see the drop down menus that follow the tables.

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH
Diet features Western/Fast Food ALIOS FPC diet
Product Code TD.88137 TD.06303 TD.160785 PWD dough

TD.190142 pellet

Fat, % Kcal 42 45 52
Fat Sources,
% by weight
21% milk fat 22% hydrogenated vegetable oil
1% soybean oil
19% hydrogenated vegetable oil
6% milk fat
4% palmitic acid
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
23% saturated
31% monounsaturated (cis)
12% polyunsaturated (cis)
34% trans
43% saturated
27% monounsaturated (cis)
7% polyunsaturated (cis)
23% trans
Sugars, % by weight 34.5% sucrose 22.4% sucrose 34.5% sucrose
Cholesterol, % by weight 0.2 0 1.25
Modifications TD.96121 1.25% cholesterol
TD.120528 Increased sucrose, 1.25% cholesterol
TD.120330 0.2% cholesterol
TD.130885 0.2% cholesterol, 27% sucrose
TD.140154 adds customer supplied palmitic acid

For high fat diet options to induce uncomplicated NAFLD see our Diet Induced Obesity page.

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome
Diet features High Fat, Cholesterol & Cholate Methionine/choline deficient (MCD)
Product Code TD.02028 TD.90262
Fat, % Kcal 42 22
Fat Sources,
% by weight
21% milk fat 10% corn oil
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
14% saturated
28% monounsaturated
58% polyunsaturated
Sugars, % by weight 33.3% sucrose 46% sucrose
Cholesterol, % by weight 1.25 0
Cholate Source, % by weight 0.5 0
Related diets TD.09237 15% milk fat, 1% cholesterol
TD.88051 Hybrid version
TD.94149 MCD control diet

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western or fast food style diets fed to induce NASH with metabolic syndrome contain 40 – 45% kcal from milkfat (a fat source high in palmitate) with added cholesterol (0.15 – 2%) and are high in sucrose (>30%). Dietary palmitate and cholesterol have both previously been associated with the progression from simple steatosis to NASH.


  • TD.88137       Adjusted Calories Diet (42% from fat)
  • TD.96121       21% MF, 1.25% Chol. Diet
  • TD.120528     42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)

Research use:

These diets can induce obesity, metabolic syndrome, and simple steatosis within nine weeks of feeding. Increased hepatic inflammation has been observed after 12 weeks of feeding. NASH typically requires longer feeding with fibrosis developing within nine months and late stage fibrosis including hepatic ballooning occurring after 14 – 20 months of feeding. Increasing dietary sucrose (~41%) and cholesterol (~1.25%) accelerates the NASH phenotype with steatosis, inflammation and hepatocyte ballooning observed within 12 weeks. In addition to feeding a high fat diet, providing a glucose/fructose mixture in the drinking water may further promote NASH development.

Select References:

Charlton, M., et al., Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol, 2011. 301(5): p. G825-34. http://www.ncbi.nlm.nih.gov/pubmed/21836057

Gores, G., Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H. Am J Physiol Gastrointest Liver Physiol, 2015. 308: p. G159. http://ajpgi.physiology.org/content/308/2/G159

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Ioannou, G.N., et al., Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis. J Lipid Res, 2009. 54(5): p. 1326-34. http://www.ncbi.nlm.nih.gov/pubmed/23417738

Alkhouri, N., et al., Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis. J Biol Chem, 2010. 285(5): p. 3428-38. http://www.ncbi.nlm.nih.gov/pubmed/19940134

Dixon, L.J., et al., Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One, 2013. 8(2): p. e56100. http://www.ncbi.nlm.nih.gov/pubmed/23409132

DeLeve, L.D., et al., Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis. Am J Pathol, 2008. 173(4): p. 993-1001. http://www.ncbi.nlm.nih.gov/pubmed/18772330

VanSaun, M.N., et al., High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol, 2009. 175(1): p. 355-64. http://www.ncbi.nlm.nih.gov/pubmed/19541928

Asgharpour, A., et al., A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol, 2016. 65(3): p. 579-88. http://www.ncbi.nlm.nih.gov/pubmed/27261415

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Tsuchida, T., et al., A simple diet-and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer. Journal of hepatology, 2018. 69(2):385-395. https://www.ncbi.nlm.nih.gov/pubmed/29572095

The American Lifestyle-Induced Obesity Syndrome (ALIOS) model involves feeding the “American fast food” diet high in trans-fats and sugar. Dietary trans-fats from hydrogenated vegetable shortening (HVO) are associated with increased insulin resistance and hepatic inflammation in rodent NASH models. In addition to diet, a glucose/fructose solution is added to the drinking water and sedentary behavior promoted by removing the overhead cage feeders in this model.


  • TD.06303       22% HVO Diet
  • TD.120330     22% HVO + 0.2% Cholesterol Diet
  • TD.130885     ALIOS with Added Sugar

Research use:

The ALIOS model develops obesity with insulin resistance, elevated ALT levels, and steatosis within 16 weeks. Increased inflammation and early development of fibrosis have been observed at 6 months. Severe steatosis with fibrosis and inflammation develops within 12 months of feeding with 50% of the mice reportedly developing hepatic neoplasms. Adding cholesterol (0.2%) to the American Fast Food diet may accelerate NASH phenotype development.

Select References:

Koppe, S.W., et al., Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol, 2009. 297(2): p. G378-84. http://www.ncbi.nlm.nih.gov/pubmed/19541924

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Mells, J.E., et al., Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol, 2012. 302(2): p. G225-35. http://www.ncbi.nlm.nih.gov/pubmed/22038829

Dowman, J.K, et al., Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle. Am J Pathol, 2014. 184(5):1550-1561. https://www.ncbi.nlm.nih.gov/pubmed/24650559 

Mells, J.E., et al., Saturated fat and cholesterol are critical to inducing murine metabolic syndrome with robust nonalcoholic steatohepatitis. J Nutr Biochem, 2014. 26(3): p. 285-92. http://www.ncbi.nlm.nih.gov/pubmed/25577467

The Fructose, Palmitate, Cholesterol and Trans-Fat (FPC) diet is a recent NASH diet that includes Western and ALIOS model diets to achieve both metabolic and hepatic NASH features within an accelerated time frame. Key features of the FPC diet include 1) a lower Met content than typical rodent diets by decreasing total protein without supplementing sulfur amino acids; 2) choline supplementation is lower than typical but is not considered deficient; 3) high in sucrose (~34% by weight); 4) 1.25% cholesterol; 5) 52% kcal from fat with fat sources including milkfat fat, palmitic acid and hydrogenated vegetable shortening to provide trans-fats. Like the ALIOS model, the FPC model also provides a glucose/fructose solution to the drinking water.


  • TD.160785     52 kcal/Fat Diet (C16:0, HVO, AMF, Choline/Met)

Research use:

Male C57BL/6J mice fed the FPC diet and provided a glucose/fructose drinking solution developed insulin resistance and NAFLD with inflammation, hepatocyte death, and fibrosis within 16 weeks.

Select References:

Wang, X., et al., Hepatocyte TAZ/WWTR1 promotes inflammation and fibrosis in nonalcoholic steatohepatitis. Cell Metab, 2016. 24(6): p. 848-62. https://www.ncbi.nlm.nih.gov/pubmed/28068223  

Zhu, C., et al., Hepatocyte Notch activation induces liver fibrosis in nonalcoholic steatohepatitis. Sci Transl Med, 2018. 10(468). https://www.ncbi.nlm.nih.gov/pubmed/30463916

Common diets to induce obesity (DIO) can be fed to induce uncomplicated NAFLD. These high fat diets typically contain 40–60% kcal from fat without supplemented cholesterol or cholate. Simple sugars such as sucrose or fructose can also be supplemented via diet or water to progress the fatty liver phenotype. Diets can be in pellet or powder/dough form depending on the formula. Some models require limited physical activity and in those cases diets can be fed inside the cage. For more information see our Diet Induced Obesity page.


  • TD.08811       45%kcal Fat Diet (21% MF, 2% SBO)
  • TD.06414       Adjusted Calories Diet (60/Fat)

Research use:

In susceptible rodent models, high fat diets are commonly used to induce NAFLD with obesity and insulin resistance common metabolic features associated with NASH in humans. However, the degree of NASH pathology (steatosis, inflammation, and fibrosis) is limited or mild and varies depending on the animal model, length of feeding, and dietary components.

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic


Originally formulated to induce mild atherosclerosis in wild-type rodents, high fat diets containing added cholesterol (1 – 1.25%) and cholate (0.5% as sodium cholate or cholic acid) have also been useful in inducing NASH. This diet option includes purified “Western” style diets with increased cholesterol and cholate and also hybrid diets. Hybrid diets were originally developed by Beverly Paigen and colleagues by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate) resulting in a diet containing ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Although a less refined approach, the hybrid diet is associated with increased gallstone formation and liver damage as compared to similar purified diets.


  • TD.02028       Atherogenic Rodent Diet
  • TD.88051       Cocoa Butter Diet and Purina Mouse Chow
  • TD.09237       15% AMF Diet (1% Chol, 0.5% NaChol)

Research use:

Atherogenic diets are able to induce varied degrees of NASH with increased hepatic inflammation with early fibrosis observed after ten weeks of feeding. However, the metabolic profile typical in human NASH (obesity with insulin resistance) is not recapitulated in this model with animals typically maintaining similar body weights as control fed groups without the development of metabolic syndrome.

Select References:

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69. http://www.ncbi.nlm.nih.gov/pubmed/2380634

Kamari, Y., et al., Lack of interleukin-1alpha or interleukin-1beta inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice. J Hepatol, 2011. 55(5): p. 1086-94. http://www.ncbi.nlm.nih.gov/pubmed/21354232

Kim, D.G., et al., Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model. J Neuroinflammation, 2016. 13: p. 1. http://www.ncbi.nlm.nih.gov/pubmed/26728181

Madrigal-Perez, V.M., et al., Preclinical analysis of nonsteroidal anti-inflammatory drug usefulness for the simultaneous prevention of steatohepatitis, atherosclerosis and hyperlipidemia. Int J Clin Exp Med, 2015. 8(12): p. 22477-83. http://www.ncbi.nlm.nih.gov/pubmed/26885230

Savransky, V., et al., Chronic intermittent hypoxia causes hepatitis in a mouse model of diet-induced fatty liver. Am J Physiol Gastrointest Liver Physiol, 2007. 293(4): p. G871-7. http://www.ncbi.nlm.nih.gov/pubmed/17690174

Methionine and choline deficient (MCD) diets are amino acid defined rodent diets deficient in methionine and choline, high in sucrose (>40% by weight) with ~10% corn oil by weight. Methionine and choline deficiency decreases fat oxidation and export of fat from the liver. Dietary sucrose is necessary for hepatic lipid accumulation and oxidation. The polyunsaturated fat in corn oil promotes hepatic lipid oxidation.


  • TD.90262       Methionine/Choline Deficient Diet


  • TD.94149       Amino Acid Control Diet

Research use:

Steatosis, increased serum alanine aminotransferase (ALT), inflammation, and hepatic fat oxidation has been observed within three weeks of feeding the MCD diet with fibrosis development after six weeks. This dietary model does not produce metabolic syndrome (an aspect of NASH in human models) and progressive weight loss (up to 40%) is associated with the MCD diet feeding.


例子:TD.90262 蛋氨酸/胆碱缺乏症饮食
管制:TD.94149 氨基酸控制饮食



Select References:

Pickens, M.K., et al., Dietary sucrose is essential to the development of liver injury in the MCD model of steatohepatitis. J Lipid Res, 2009. 50(10):2072-82.  http://www.ncbi.nlm.nih.gov/pubmed/19295183

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Lee, G.S., et al., Polyunsaturated fat in the methionine-choline-deficient diet influences hepatic inflammation but not hepatocellular injury. J Lipid Res, 2007. 48(8): p. 1885-96. http://www.ncbi.nlm.nih.gov/pubmed/17526933

Vetelainen, R., A. van Vliet, and T.M. van Gulik, Essential pathogenic and metabolic differences in steatosis induced by choline or methione-choline deficient diets in a rat model. J Gastroenterol Hepatol, 2007. 22(9): p. 1526-33. http://www.ncbi.nlm.nih.gov/pubmed/17716355

Leclercq, I.A., et al., Intrahepatic insulin resistance in a murine model of steatohepatitis: effect of PPARgamma agonist pioglitazone. Lab Invest, 2007. 87(1): p. 56-65. http://www.ncbi.nlm.nih.gov/pubmed/17075577

Kashireddy, P.R. and M.S. Rao, Sex differences in choline-deficient diet-induced steatohepatitis in mice. Exp Biol Med (Maywood), 2004. 229(2): p. 158-62. http://www.ncbi.nlm.nih.gov/pubmed/14734794

Dixon, L.J., et al., Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest, 2012. 92(5): p. 713-23. http://www.ncbi.nlm.nih.gov/pubmed/22411067

Dietary models of NAFLD/NASH continue to evolve with the goal of more accurately recapitulating both the metabolic and hepatic symptoms of human disease. Commonly researchers are studying the synergistic effects of various NASH dietary features to accelerate progression of the model and severity of liver disease.

A Teklad nutritionist can work with you to formulate new diets in order to investigate novel dietary models of NAFLD/NASH.

The choice of control diet is dependent on the specific research goal. Many researchers choose to compare their NAFLD/NASH diet-fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diet or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens).

Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component. In some cases, such as those studies feeding amino acid defined diets like the MCD model, a matched control diet is recommended given the very different formulations and protein sources of grain-based diets.

When making inferences about specific nutrients within the diet an ingredient matched, low fat control diet may be necessary. There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined and digestible) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible).

A very basic purified control diet would be AIN-93M TD.94048 or AIN-93G TD.94045. AIN-93 diets have a moderate amount of sucrose at ~10% with fat from soybean oil providing a healthy fatty acid profile.

Contact a nutritionist for an additional information and control diet recommendations.




一种非常基本的纯正控制饮食将是-9300万。TD.94048或者是93g TD.94045。AIN-93日粮中含有适量的蔗糖~10%,大豆油中的脂肪提供了健康的脂肪酸谱。

Need more information? A Teklad nutritionist will work with you to determine if existing diets will meet your needs or formulate new diets to help you investigate novel dietary models of NAFLD/NASH. Contact us for a diet consultation.

Harlan teklad饲料常见问题以及使用方法









  • 凉爽和干燥;在华氏70度或以下,湿度低于50%的理想值,但高达65%是可以接受的。
  • 清洁无害虫
  • 在原始包装中或容器中,防止连续暴露于光线下,并将空气暴露在最小范围内。

Envigo提供辐照饮食,床上用品和浓缩物品。辐照通过使产品暴露于钴-60的伽马电离辐射,减少产品所含生物的数量。受伽马射线照射的生物体在分子水平上受到破坏,往往具有致命的影响。最终的结果是产品中可存活微生物数量的减少。Envigo以最低剂量2.0 mrad(20 KGy)和最大5.0mrad(50 KGy)照射所有Teclad产品。



“自由”一词意味着完全和完全不存在植物雌激素。这将排除任何由天然成分组成的饮食。唯一一种完全不含植物雌激素的饮食是不含大豆蛋白的纯化饮食。然而,Envigo提供了四种Teclad全球啮齿动物饮食,不包括已知含有大量植物雌激素的紫花苜蓿粉和豆粕等成分。2014、2016、2019和2020倍的饮食虽然不是完全“免费”,但最好用最小的植物雌激素饮食来形容。每季度对大豆异黄酮(大豆植物雌激素)的饮食进行测试时,通常会发现含量从不到可检测到的到百万分之20不等。从某种角度来看,大多数传统的啮齿动物饮食中含有200到500 ppm的异黄酮。植物雌激素最低限度饮食,如2014、2016、2019和2020 X,在植物雌激素引起关注的研究中通常是可以接受的。
















屏障设施或某些动物模型需要无菌饮食。大多数自定义的研究饮食是经不起高压灭菌的,然而,我们可以安排合格的饮食被辐照。照射剂量范围为2~5次MRAD,即20~50 kGy.涉及象征性费用和额外时间。





“无维生素”试验酪蛋白是从普通酪蛋白中提取的一种醇提酪蛋白,通常含有90%-91%的蛋白质(%Nx 6.38)、0.1%的脂肪和4-6%的水分。醇提物减少脂肪、脂溶性维生素和一些B族维生素。这种蛋白质来源最适合用于维生素缺乏饮食,当研究人员想最小化“背景脂肪”在节食中。我们已经准备了多年的这种特殊原料,并继续提供给许多实验室。我们将根据大量需求制定特殊价格。对于1,000公斤或以上的订单,建议提前通知。如果您需要这种成分,请考虑Envigo作为您的首选来源。







没有。根据NIH OACU关于在实验动物中使用非药物级化学品/化合物的指南(2008年),用于便利使用一种化合物的车辆与制剂中的活性化合物一样重要。饮食作为一种交通工具,无论是作为整体还是作为其部件的总和,都不符合本文件所定义的药品等级标准。






实验室惯例会引起动物压力。(2004年)。巴尔科姆,J.P.,Barnard,N.D.和Sandusky,C.考虑顶级实验室Anim Sci 43,42-51。

No. According to the NIH OACU Guidelines for Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals (2008), the vehicle used to facilitate administration of a compound is as important of a consideration as the active compound in the preparation. Diet as a vehicle, either whole or as the sum of its parts, would not meet the standard for pharmaceutical grade as defined by this document.

You may have been asked this question by your IACUC due to a passage in the 8thedition of the Guide for the Care and Use of Laboratory Animals (2011) stating that pharmaceutical-grade chemicals and other substances should be used, when available, for all animal-related procedures (page 31). In general, deviations from “Must” or “Should” statements within the Guide require justification and approval by your institutional IACUC.

Alternative dosing techniques (gavage, injection) can introduce stress from handling and increase the risk for potential injury. Stress can affect animal well-being as well as scientific parameters through modifications in behavior and physiology. Dosing via diet is an established method for delivering test compounds to animals that is non-invasive, reducing stress and potential injury from handling. Therefore, use of non-pharmaceutical grade compounds as a delivery method is justified, as it is advantageous for both animal welfare and experimental design.

Further reading:

Guide for the Care and Use of Laboratory Animals: 8th Edition (2011). National Research Council Committee for the Update of the Guide for the Care and Use of Laboratory Animals.

Guidelines for the Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals. (2008). Animal Research Advisory Committee, Office of Animal Care and Use, NIH.

Laboratory routines cause animal stress. (2004). Balcombe, J.P., Barnard, N.D., and Sandusky, C. Contemp Top Lab Anim Sci 43, 42-51.

Harlan TD.07108 Zn Defic. Diet (0.5) 锌缺乏饲料使用说明书

Harlan TD.07108 Zn Defic. Diet (0.5) 锌缺乏饲料使用说明书

Harlan Teklad动物饲料

TD.85419  is 0.5 – 1.5 ppm,Diet TD.07108 is a modification of TD.85419 with 0.5 ppm added zinc as zinc sulfate and purple food coloring. Total zinc is estimated to be 2 – 3.5 ppm (background + added zinc)

TD.85419是0.5 – 1.5 ppm,饮食TD.07108是TD.85419的改良形式,添加了0.5 ppm的锌作为硫酸锌和紫色食用色素。 总锌估计为2-3.5 ppm(基础锌含量+添加的锌)

Harlan Teklad Custom Diet
TD.07108 Zn Defic. Diet (0.5)

Formula g/Kg
Egg White Solids, spray-dried 200
Dextrose, monohydrate 634.2376
Corn Oil 100
Cellulose 29.986
Mineral Mix, Zn Deficient (81264) 25.6902
Chromium Potassium Sulfate, dodecahydrate 0.02
Zinc Sulfate, heptahydrate 0.0022
Vitamin Mix, Teklad (40060) 10
Biotin 0.004
Ethoxyquin, antioxidant 0.02
Purple Food Color 0.04


A zinc deficient diet with 0.5 ppm added zinc as zinc sulfate and purple food coloring. Total zinc estimated to be 2 – 3.5 ppm (background + added zinc). Modified from TD.85419. For the series TD.07108-TD.07110.

Selected Nutrient Information1

% by weight % kcal from
Protein 16.1 16.6
Carbohydrate 58.3 60.1
Fat 10.0 23.2
Kcal/g 3.9
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Adjusted Zinc
  • Color Coded
  • Zinc Sulfate

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated