Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan TD.88137 高酯饲料Adjusted calories diet (42% from fat)说明书

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

A staple of atherosclerosis research

More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model.

With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.

Formula g/Kg
Casein 195.0
DL-Methionine 3.0
Sucrose 341.46
Corn Starch 150.0
Andydrous Milkfat 210.0
Cholesterol 1.5
Cellulose 50.0
Mineral Mix, AIN-76 (170915) 35.0
Calcium Carbonate 4.0
Vitamin Mix, Teklad (40060) 10.0
Ethoxyquin 0.04

Selected nutrient information1

  % By weight % kcal from
     
Protein 17.3 15.2
Carbohydrate 48.5 42.7
Fat 21.2 42.0
     
kcal/g 4.5  
Cholesterol2 0.2%  
  • Values are calculated from ingredient analysis or manufacturer data.
  • 15% added, 0.05% from fat source.

Critical dietary features of TD.88137 related to atherosclerosis development include:

++Cholesterol (0.2% total cholesterol)

++Total fat (21% by weight; 42% kcal from fat)

++High in saturated fatty acids

(>60% of total fatty acids)

++High sucrose (34% by weight)

Typical fatty acid profile of TD.88137

Typical fatty acid analysis, Mean SD  
% of diet1  
     
Total 20.7 1.5  
Saturated fat 12.8 0.8  
Monounsaturated fat 5.6 0.5  
Polyunsaturated fat 1.0 0.2  
Unknown2 1.3 0.3  
       
Typical fatty acid profile, Mean SD  
% of total fatty acids1  
     
Saturated fat 61.8 2.0  
Monounsaturated fat 27.3 2.1  
Polyunsaturated fat 4.7 0.8  
4:0 2.1 1.1  
6:0 1.5 0.7  
8:0 1.1 0.3  
10:0 2.6 0.5  
12:0 3.3 0.5  
14:0 10.6 0.9  
16:0 28.9 1.3  
16:1 1.5 0.2  
18:0 12.5 0.8  
18:1 (Oleic) 20.9 2.6  
18:1 Isomers3 4.0 1.2  
18:2 (Linoleic) 2.3 1.0  
18:2 Isomers4 1.3 0.5  
18:3 (Linolenic) 0.7 0.2  
  • n = 21, analysis conducted by two independent laboratories.
  • Unidentified fatty acids and those contributing on average less than 0.5% of total fatty acids.
  • Includes trans isomers elaidic and vaccenic acid and unidentified cis isomers.
  • Includes trans isomers.

Key points from the literature

TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:

++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks (2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).

++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic arch (6, 7).

For further information about TD.88137, or if you are interested in learning more about other atherogenic or high fat diets contact us at

With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis, hypertension and metabolic syndrome. Contact us for a more extensive reference list.

Control diet options for TD.88137

Natural ingredient diets

++Also referred to as standard diets or chow

++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared to TD.88137

++Limits inferences to differences in dietary pattern versus a specific dietary component

Ingredient matched, low fat diets

++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients

++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at

Suggested ingredient matched, low fat controls

Diet kcal/g Fat, % by % kcal Fat sources, Sucrose, %
    weight from fat % by weight by weight
        3.7% milk fat,  
TD.05230 3.7 5.2 12.6 1.3% soybean 34.1
        oil  
           
        3.7% milk fat,  
TD.08485 3.6 5.2 13.0 1.3% soybean 12.0
        oil  
           

Additional controls are available. Contact a nutritionist at

Key planning information:

  • Store diet refrigerated and plan to use within six months. Diet should be replaced at minimum once per week when fed on cage tops.
  • Diets available as a soft ½” pellet or as a crumbly powder.
  • Three kg minimum order quantity. For planning purposes, estimates for diet uses (including feed intake and diet waste) are 5 g of diet per mouse and 30 g of diet per rat per day.
  • Two-day lead time for orders less than 10 kg. Two-week lead time for larger quantity orders.
  • Lead time for irradiation adds two weeks for any quantity of diet and must be requested at the time you place your order. Changes in texture and browning may occur with irradiation.
  • Shipping can affect pellet quality. Vacuum packaging can offer protection of the pellets during shipping. Two-day shipping is recommended during warmer months.

Contact us to place an order,

References

  1. Plump, A.S., et al.,Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells. Cell, 1992. 71(2): p. 343-53.
  2. Nakashima, Y., et al.,Apoe-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994. 14(1): p. 133-40.
  3. Febbraio, M., et al.,Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.

J Clin Invest, 2000. 105(8): p. 1049-56.

  1. Nakashima, Y., et al.,Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb

Vasc Biol, 1998. 18(5): p. 842-51.

  1. Towler, D.A., et al.,Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.

J Biol Chem, 1998. 273(46): p. 30427-34.

  1. Tsuchiya, K., et al.,FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
  2. Huszar, D., et al.,Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1. Arterioscler Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
  3. Yang, B., et al.,Changes of skeletal muscle adiponectin content in diet-induced insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
  4. Schafer, K., et al.,Leptin promotes vascular remodeling and neointimal growth in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
  5. Lijnen, H.R., et al.,Nutritionally induced obesity is attenuated in transgenic mice overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc Biol, 2003. 23(1): p. 78-84.
  6. Maquoi, E., et al.,Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002. 51(4): p. 1093-101.
  7. VanSaun MN, et al. High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol 175:355-64.
  8. Dixon LJ, et al. Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One 8:e56100.

Harlan Teklad动物饲料分类

Harlan Teklad动物饲料分类

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Standard natural ingredient diets  标准天然成分饮食

  • Rodent diets  啮齿类食物
  • Dog diets  狗粮
  • Rabbit diets   兔饲料
  • Guinea pig diets    豚鼠日粮
  • Primate diets  灵长类饮食
  • Swine diets  猪日粮
  • Other diets

Custom research diets  定制研究饮食

  • Ordering and services  订购和服务
  • NAFLD and NASH    NAFLD和NASH
  • AIN diet formulas   AIN饮食配方
  • Atherogenic    动脉粥样硬化

Atherogenic

Diet can be a useful tool to induce or accelerate atherosclerosis in laboratory animal models. Key dietary features used to induce atherosclerosis in rodents vary depending on the research model, desired endpoint, and length of feeding. While formulations of atherogenic diets continue to evolve, the options that are well-described in the literature are summarized below. For more information on each diet option and literature references see the expandable tabs following the diet table.

Research use Key dietary features Examples
“Western” purified atherogenic diet
Accelerated hypercholesterolemia and plaque formation in genetically modified models such as Apoe and Ldlr deficient mice.

Used for diet induced obesity in a variety of rodent models.

  • High fat diet (20 – 23% by weight; 40 – 45% kcal from fat)
  • Saturated fatty acids (SFA >60% of total fatty acids)
  • Milkfat/butterfat
  • Sucrose (34% by weight)
  • Cholesterol (0.2% total)
TD.88137
TD.10885
“Western” purified atherogenic diet with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

  • High fat diet (15 – 20% by weight; 34 – 45% kcal from fat)
  • Saturated fatty acids (SFA >55% of total fatty acids)
  • Milkfat/butterfat, cocoa butter
  • Sucrose (30-50% by weight)
  • Cholesterol (1 – 1.25%)
  • Cholate Source (0.5%)*
TD.02028
TD.09237
Hybrid high fat diets with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

  • 75% rodent breeder diet; 25% purified ingredients
  • High fat (~15% by weight; 37% kcal from fat)
  • Saturated fatty acids (SFA >45% of total fatty acids)
  • Cholesterol (1.25%)
  • Cholate source (0.5%)*
TD.88051
TD.90221
Standard diets with added cholesterol
Induce hypercholesterolemia in genetically modified and wild type models without promoting obesity.
  • Standard, grain-based rodent diet
  • Minimal/moderate phytoestrogen diets recommended
  • Cholesterol (1 – 4%)
TD.120097
TD.07841
TD.01383

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available.

  • Diet induced obesity    饮食诱导肥胖
  • Fat/lipid adjusted   脂肪/脂调节
  • Carbohydrate adjusted    碳水化合物调节
  • Protein adjusted diets  蛋白质调节饮食
  • Vitamin adjusted diets  维生素调整饮食
  • Mineral adjusted diets  矿物调节饮食
  • NaCl adjusted (natural ingredient)  盐调节(天然成分)
  • Amino acid defined  氨基酸定义
  • Doxycycline diets  多西环素饮食
  • Tamoxifen diets  他莫昔芬饮食
  • Customer supplied ingredients  客户供应的配料
  • Basal mixes  基混合体
  • Isoflavone adjusted  异黄酮调节
  • Rabbit, swine and other species  兔子、猪和其他物种
  • Diet ingredients  饮食成分

Medicated diets  药物饮食

Teklad medicated lab animal diets are manufactured either at a subsidiary plant or at our custom research diet facility. Contact customer service or your local Envigo account representative for pricing and availability of stocked diets. Contact a nutritionist to discuss customized versions of these or other medicated diets.

Medicated diets must be used as directed by a veterinarian.

Stocked medicated rodent diets
Primary Purpose Product Code Medication Concentration Base diet Packaging
Fur mite treatment TD.130755 Ivermectin 12 ppm 2018 25 lb bag- irradiated
Pinworm treatment TD.01432 Fenbendazole 150 ppm 2018S 25 lb bag- irradiated*
50 lb bag- autoclavable
Pneumocystis pneumonia
prevention
TD.06596 Uniprim® 275 ppm trimethoprim
1365 ppm sulfadiazine
2018 25 lb bag- irradiate

Harlan Teklad TD.120240 Doxycycline饲料 40 ppm Doxy Diet (2018, B)

Harlan Teklad TD.120240 Doxycycline饲料 40 ppm Doxy Diet (2018, B)

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.704
Doxycycline Hyclate 0.046
Blue Food Color 0.25

Key Features

  • Standard Diet Base
  • 2018
  • Doxycycline
  • Color Coded Blue

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week  More frequent replacement may be advised
  • Lead time:    2 weeks non-irradiated     4 weeks irradiated

Footnote

Modification of TD.10483 to add blue coloring. Designed to add 40 mg doxycyline/kg diet using the Teklad 2018 Global Rodent Diet as the base. Doxycycline hyclate contains approximately 87% doxycycline.

Selected Nutrient Information1

  % by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Options (Fees Will Apply)

  • Rush order (pending availability)
  • Irradiation (see Product Specific Information)
  • Vacuum packaging (1 and 2 Kg)

Harlan Teklad Medicated diets 药物饲料

Harlan Teklad Medicated diets 药物饲料

Harlan Teklad代理,Harlan Teklad一级代理,欢迎访问Harlan Teklad官网或者咨询我们获取更多相关产品信息。

Teklad medicated lab animal diets are manufactured either at a subsidiary plant or at our custom research diet facility. Contact customer service or your local Envigo account representative for pricing and availability of stocked diets. Contact a nutritionist to discuss customized versions of these or other medicated diets.

特克拉德药物实验室动物饮食是在一个附属工厂或在我们的定制研究饮食设施生产。与客户服务部门或当地Envigo客户代表联系,了解库存饮食的定价和供应情况。联系营养师讨论这些或其他药物饮食的定制版本。

Medicated diets must be used as directed by a veterinarian.

Stocked medicated rodent diets
Primary Purpose Product Code Medication Concentration Base diet Packaging
Fur mite treatment TD.130755 Ivermectin 12 ppm 2018 25 lb bag- irradiated
Pinworm treatment TD.01432 Fenbendazole 150 ppm 2018S 25 lb bag- irradiated*
50 lb bag- autoclavable
Pneumocystis pneumonia
prevention
TD.06596 Uniprim® 275 ppm trimethoprim
1365 ppm sulfadiazine
2018 25 lb bag- irradiated*
COLLAPSE ALL TABS

  • Testing shows very little medication loss due to manufacturing
  • Recommended storage at or below 70 degrees fahrenheit with humidity at or below 65%
  • Durable cylindrical pellets are visually distinct from flattened oval or extruded pellets (see picture)
  • 2018 supports growth and reproduction
  • 2018 is closely related to other Global Rodent Diets, and shares some commonality with traditional rodent diets

The anthelmintic ivermectin is used for the treatment of fur mites. TD.130755 (Irradiated 2018, 12 ppm Ivermectin) is stocked in 25 lb bags. Contact customer service regarding availability of TD.130755.

The anthelmintic fenbendazole is used for treatment of pinworms. TD.01432 (2018S, 150 ppm fenbendazole) is stocked in 25 lb bags that are irradiated or in 50 lb bags that can be autoclaved (note autoclaving reduces fenbendazole concentration and is only recommended if using to treat mice). Contact customer service regarding availability ofTD.01432.

Uniprim® contains trimethoprim and sulfadiazine, used to prevent occurrences of Pneumocystis pneumonia in immunocompromised mice and rats. TD.06596 (Irradiated 2018, 4100 ppm Uniprim®) is stocked in 25 lb bags. Contact customer service regarding availability of TD.06596.

Custom diets with Ivermectin, Uniprim®, and Fenbendazole are available. Modifications include: different base diet, coloring with food dyes, and other dosing concentrations based on your unique requirements. Contact a nutritionist to discuss your needs.

Other medications and compounds such as doxycycline and tamoxifen may be added to diets at our custom research diet facility. Visit our custom research diets site or contact us

药物饮食必须按照兽医的指示使用。

含药啮齿动物饲料
主要目的 产品代码 药物治疗 浓度 基础饮食 包装
毛螨处理 TD.130755 伊维菌素 百万分之十二 2018 25磅包-辐照
粉螨治疗 TD.01432 芬苯达唑 百万分之150 2008 S 25磅包-辐照*
50磅包-高压釜
肺孢子虫p肺炎
预防
TD.06596 Uniprim® 275 ppm甲氧苄啶
1365 ppm磺胺嘧啶
2018 25磅包-辐照*
COLLAPSE ALL TABS

  • 测试显示制造过程中药物损失很小。
  • 建议贮存温度在华氏70度或以下,湿度在65%或以下
  • 耐用的圆柱形球团在视觉上有别于扁平的椭圆形或挤压球团(见图)
  • 2018支持生长和繁殖
  • 2018与其他全球啮齿动物饮食密切相关,并与传统的啮齿动物饮食有一些共同之处

这个驱虫药伊维菌素用于治疗毛皮螨。TD.130755(辐照2018年,12 ppm伊维菌素)储存在25磅袋。与客户服务部门联系,了解TD.130755.

本发明为驱虫药芬苯达唑。TD.01432(2018S,150 ppm芬苯达唑)储存在25磅经辐照的袋子中,或装在50磅可被压的袋子中(注意,高压灭菌可以降低芬苯达唑的浓度,只有当用于治疗小鼠时才推荐使用)。与客户服务部门联系,了解TD.01432.

Uniprim®含有甲氧苄啶和磺胺嘧啶,用于预防免疫受损小鼠和大鼠肺炎肺孢子虫肺炎的发生。TD.06596(2018年辐照,4100 ppm Uniprim)®)装在25磅重的袋子里。与客户服务部门联系,了解TD.06596.

伊维菌素定制饮食®,芬苯达唑是可行的。修改包括:不同的基础饮食,染色与食物染料,以及其他剂量的浓度,根据您的独特要求。联系营养师来讨论你的需求。

其他药物和化合物,如强力霉素和他莫昔芬可以添加到我们的定制研究饮食设施的饮食。访问我们的定制研究饮食网站或联系我们以获得更多信息。

Harlan Teklad 他莫昔芬动物饲料 Tamoxifen diets

Harlan Teklad 他莫昔芬动物饲料 Tamoxifen diets

Diets with tamoxifen activate CreER

Several references cite use of tamoxifen-containing diets for models with Cre under the control of a mutated estrogen receptor. Envigo Teklad diets makes a variety of tamoxifen-containing diets customized to meet your research needs. We also make a variety of doxycycline-containing diets for tet regulated systems. If you have any questions, don’t hesitate to consult a nutritionist.

Teklad stocks tamoxifen and tamoxifen citrate

Tamoxifen is added to the diet as a tamoxifen-sucrose mixture. Users are encouraged to use the stocked supply. However, Teklad will continue to work with customer prepared mixes. If you choose this option, please consult a nutritionist for additional information about preparing and sending the mixture.

Users are encouraged to select from the stocked options below supply readily available to ship within days.

Teklad offers diet with USP grade tamoxifen and tamoxifen citrate
Tamoxifen
mg per kg diet
Daily tamoxifen
mg per kg body weight1
Diet code
(Teklad supplied tamoxifen)
    Natural colored Red
250 40 TD.130855 TD.130856
500 80 TD.130857 TD.130858
400 citrate2 40 TD.130859 TD.1308603

1 Assumes 20-25 g body weight and three-four g intake
2 Tamoxifen citrate is 66% tamoxifen
3 Referred to as TAM400/CreER in Europe (TD.55125)

Please add 5-10 day lead time for irradiated diets; minimum 3 kg orders are required for stocked and customized items.

These diets all have tamoxifen premixed with ~five percent sucrose as a palatability enhancer; however, feed aversion may still occur. If intake is a problem, see below for advice.

Teklad Global 2016 — base diet in these examples — is only one of several minimal phytoestrogen diets Teklad produces. Consult a nutritionist about use of other base diets.

COLLAPSE ALL TABS

  • Minimum order quantity is three kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
  • Irradiation (optional) minimal dose of 20 kGy (Must be requested at time of order)
  • To place an order: contact Teklad Customer Service at 800.483.5523 or initiate order online

Plan for:

  • Initial reduction in food intake and weight loss
  • Daily observation of animal tolerance — consult your IACUC for monitoring metrics
  • Intervene when necessary

If intake seems to be a problem:

  • Determine if a lower dose is effective for your model by conducting a tamoxifen dose feeding trial
  • Gradually acclimate mice by mixing tamoxifen-containing pellets with regular feed pellets
  • Wet the food, then place in a dish inside the cage (requires daily replacement)
  • Feed tamoxifen diet on weekdays, regular diet on weekends
  • Alternate weeks (two weeks on tamoxifen diet, one week off) for longer treatments
  • Tamoxifen is a selective estrogen receptor modulator (SERM) meaning it can repress actions of estrogen or have pro-estrogen effects
  • Usual dose for therapeutic effects in humans is 20 mg/day. Tamoxifen diets for rodents typically contain ~one to two mg/pellet
  • Accidental tamoxifen exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of tamoxifen containing diets.
  • Length of treatment varies from one to two weeks1,2,5,6,7,8 to one to two months3,4,8
  • Pure tamoxifen1,5,8 and tamoxifen citrate1,2,3,4,6,7 are both effective
  • Usual tamoxifen doses are ~40-80 mg per kg body weight per day
  • Typical inclusion for pure tamoxifen is 250 mg8 or 500 mg1,5 and for tamoxifen citrate is 400 mg per kg diet1,2,3,4,6,7
  • Initial weight loss of ten % is reported1,2,4,7,8, associated with reduced food intake2
  • Subsequent recovery of body weight after returning to regular diet may be compromised by gene inactivation2,7
  1. Andersson KB, Winer LH, Mork HK, Molkentin JD, Jaisser F. 2010. Tamoxifen administration routes and dosage for inducible Cre-mediated gene disruption in mouse hearts. Transgenic Res 19:715-725.
  2. Chiang PM, Ling J, Jeong YH, Price DL, Aja SM, Wong PC. 2010. Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. Proc Natl Acad Sci USA 107:16320-16324.
  3. Kardakaris R, Gareus R, Xanthoulea S, Pasparakis M. 2011. Endothelial and macrophage-specific deficiency of P38alpha MAPK does not affect the pathogenesis of atherosclerosis in ApoE-/- mice. PLoS One 6:e21055.
  4. Kiermayer C, Conrad M, Schneider M, Schmidt J, Brielmeier M. 2007. Optimization of spatiotemporal gene inactivation in mouse heart by oral application of tamoxifen citrate. Genesis 45:11-16.
  5. Koitabashi N, Bedja D, Zaiman AL, Pinto YM, Zhang M, Gabrielson KL, Takimoto E, Kass DA. 2009. Avoidance of transient cardiomyopathy in cardiomyocyte-targeted tamoxifen-induced MerCreMer gene deletion models. Circ Res 105:12-15.
  6. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. 2009. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. PCirc Res 106:559-572.
  7. Miro-Murillo M, Elorza A, Soro-Arnaiz I, Albacete-Albacete L, Ordonez A, Balsa E, Vara-Vega A, Vazquez S, Fuertes E, Fernandez-Criado C, Landazuri MO, Aragones J. 2011. Acute Vhl gene inactivation induces cardiac HIF-dependent erythropoietin gene expression. PLoS One 6:e22589.
  8. Welle S, Burgess K, Thornton CA, Tawil R. 2009. Relation between extent of myostatin depletion and muscle growth in mature mice. Am J Physiol Endocrinol Metab.Oct;297(4):E935-40.

Harlan 强力霉素饲料 Doxycycline diets

Harlan 强力霉素饲料 Doxycycline diets

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Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1 – 2 days (non-irradiated) or 1 – 2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don’t find a suitable option below.

EnvigoTeclad饮食使各种多西环素包含饮食为您的Tet调节系统。TD.01306以625毫克/千克的强力霉素添加到2018年全球啮齿动物饲料中,通常可在1至2天(未辐照)或1-2周(辐照)内装运。饮食很容易定制。定制规格包括:基础饮食,多西环素浓度和食用色素。联系我们如果你在下面找不到合适的选择的话,来讨论你的需求。

Formula examples with Teklad global 2018 base diet
Doxycycline hyclate1 USP (mg/kg diet) Natural color Alternatives with food coloring
mg per kg diet
46 TD.10483 TD.180327 Red
TD.120240 Blue
100 TD.04233 TD.130840 Blue
200 TD.00502 TD.180625 Red
TD.150431 Blue
TD.04104 Green
625 TD.01306
stocked2
TD.08541 Red
TD.120769 Blue
TD.09628 Green
1000 TD.05298 TD.06294 Red
TD.120658 Blue
2000 TD.05512 TD.140011 Red
TD.09633 Green
6000 TD.01533

1 Doxycycline hyclate is ~87% doxycycline
2 TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Considerations when choosing a base diet

    • Teklad Global Rodent Diets® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
    • Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.
Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate1 at 625 mg/kg diet
Base diet Natural color Alternatives with food coloring
mg per kg diet
2014 TD.00426 TD.07382 Red
TD.07573 Yellow
2016 TD.02503 TD.140263 Blue
TD.170364 Red
2019 TD.09651 TD.09761 Green
2020 TD.110720 TD.130141 Red

1 Doxycycline hyclate is ~87% doxycycline

Advantages of diet delivery of doxycycline

  • Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
  • Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
  • Diet is typically changed once per week. Water may need to be changed every few days
  • Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

Key planning information

  • Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
  • Store diet refrigerated and plan to use within six months
  • Typical lead time is two weeks (four weeks if irradiated)
    • TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation
  • Irradiation (20 – 50 kGy) is optional, and must be requested at time of order

Safe handling of doxycycline diets

  • Doxycycline is a tetracycline class antibiotic.
  • Usual dose for therapeutic effects in humans is 100-200 mg/day. Doxycycline diets for rodents typically contain ~2 mg/pellet.
  • Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
  • Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

多西环素饮食给药的优势

  • 典型的多西环素浓度可以用于饮食和水。低浓度的多西环素可能足够,625毫克/千克的饮食是最常见的浓度。
  • 饮食能保护身体免受光线伤害。水可能必须用深色或铝箔包装的瓶子浇水。
  • 饮食通常每周改变一次。水可能需要每隔几天更换一次。
  • 饮食供应也可以减少脱水的风险,并避免需要给予蔗糖(考索恩等)。艾尔。(2007年)

关键规划信息

  • 最小订货量为3公斤,足以喂养20只小鼠一个月。
  • 储存冷冻饮食,并计划在六个月内使用。
  • 典型的准备时间为两周(如果经过辐照,则为四周)
    • TD.01306通常在未经辐照的2天内或在辐照的2周内装运。
  • 辐射(20-50 kGy)是可选的,必须按顺序要求。

多西环素饮食的安全处理

  • 强力霉素是四环素类抗生素。
  • 人体治疗效果的一般剂量为每天100至200毫克。用于啮齿动物的多西环素饮食通常含有~2毫克/粒。
  • 当处理饮食时,使用典型的实验室预防措施,如实验室外套、手套和口罩,可以将多西环素意外暴露量降到最低。
  • 您的化学安全部门应联系,以获得额外的机构特定的指导方针,以处理和处置多西环素的饮食。

Harlan饲料问与答 Diet FAQs

Harlan饲料问与答 Diet FAQs

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Standard Natural Ingredient Diets

A more complete name would be Standard Natural Ingredient Diets. These diets are manufactured in large quantities (at least 2 tons) and stocked at one or more Envigo Teklad distribution centers. Standard natural ingredient diets are readily available and can generally be delivered within a few days after an order is placed. These diets primarily contain grains (corn, wheat), legumes (soybean meal) and to a lesser extent ingredients such as fish meal, and meat and bone meal. Standard natural ingredient diets include Global and Traditional diets and are available Irradiated and/or Certified.

Using standardized fixed formulas and ingredients purchased under the same stringent specifications in the United States and Europe, Envigo’s unique Teklad Global Diets Program provides nutritional consistency to national and multinational companies for their worldwide research, maximizing global study uniformity. Global Diets have the same product names in all countries. The Global Rodent diets have lower protein levels than most traditional diets and are formulated free of alfalfa with zero or modest amounts of soybean meal, thus minimizing the presence of natural phytoestrogens.

Envigo offers a variety of Traditional Teklad diets for the common laboratory animal species. These fixed formula diets are made from high quality ingredients. While traditional diets will supply the known nutrient needs of laboratory animals, we recommend that researchers consider the use of a diet from our Global Diet line. The traditional rodent diets were formulated decades ago based on understanding of rodent nutrition, ingredients, and diet manufacturing at the time. There was limited appreciation of the effects of non-nutrients, such as phytoestrogens, on experimental results. Alfalfa meal and soybean meal are the major sources of phytoestrogens in laboratory animal diets. These ingredients, particularly soybean meal, are found in most traditional rodent diets.

Fixed Formula diets are diets that are manufactured in accordance with a formula that remains unchanged from one production to the next. In conjunction with strict quality standards for raw materials, this approach ensures quality and consistency by minimizing nutrient variability and the variability of other phytochemicals in the diet which might affect a research study.

Certified diets are diets that have been tested for a standard panel of environmental contaminants that are known to be capable of interfering with a study. These diets help ensure that a study complies with the FDA’s Good Laboratory Practices regulations requiring periodic feed analysis. An Envigo certificate of analysis signed by a nutritionist is made available to the customer.

Our #1 goal is to provide quality and consistent products to our customers. This begins with ensuring that raw materials of only the highest consistency and quality are obtained. Raw materials are only obtained from approved suppliers who agree to meet our quality standards. Suppliers are audited on a regular basis and their products are tested for quality before being accepted into our facilities. Fixed formulas and established manufacturing procedures are in place for each product which ensure that the product is manufactured consistently with each production. In-process and final product testing is completed prior to distribution of the product to a customer. Envigo utilizes its own network of company owned and operated distribution centers throughout the US and Canada rather than use outside distributors.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. The common guideline of a 6 month shelf life is based on longstanding practice in North America. In Europe and Asia, differences in local practices and regulatory oversight have led to Teklad standard natural ingredient diets being routinely used out to 9 months and sometimes 12 months post-manufacture. This practical experience, along with literature support and vitamin testing over time, gives us confidence that these diets continue to support animal health and study integrity out to at least 9 months post-manufacture. Please refer to your institution for guidance if you are unsure of local policies.

没有一个人能够预测当特定的饮食会破坏或缺乏一个或更多的营养。在常见的 6 个月的货架寿命是基于长期的实践中。在欧洲和亚洲 , 不同地方的做法和监管标准 , 导致 Teklad 饮食天然成分通常使用了 9 个月和 12 个月后有时制造。这种实践的经验 , 再加上文学和测试支持 , 让我们继续支持这些膳食研究动物的健康和完整性至少 9 个月后制造。请参考您的机构的指导 , 如果你不确定的地方政策。

Recommended storage conditions:

  • Cool and dry; at or below 70 degrees Fahrenheit, humidity below 50% ideal, but up to 65% is acceptable
  • Clean and free of pests
  • In original packaging or in a container that prevents continuous exposure to light and minimal exposure to air

建议贮藏条件 :

  • 冷却和干燥 ;在等于或低于 70 华氏度 , 湿度低于 50% , 但高达 65 % 是可接受的
  • 干净 , 没有害虫
  • 在原包装或容器 , 防止在连续的光暴露和最小暴露于空气中

Envigo offers irradiated diets, bedding, and enrichment items. Irradiation reduces the bioburden (number of organisms) contained by a product by exposing the product to gamma ionizing radiation from cobalt-60. Organisms exposed to gamma irradiation are damaged at the molecular level often with lethal effects. The net result is a decrease in the amount of viable microorganisms present in the product. Envigo irradiates all Teklad products at a minimum dose of 2.0 Mrad (20kGy) and a maximum of 5.0 Mrad (50 kGy).

Autoclaving destroys vitamins and amino acids that are supplemented in the diet. To compensate for this destruction, autoclavable diets contain an additional supplement of vitamins and supplemental amino acids. Autoclavable diets are packaged in perforated bags which allow steam from the autoclave to penetrate throughout the diet when the diet is autoclaved inside the bag.

Phytoestrogens are phytochemicals naturally produced by plants which interact with endogenous estrogens. They have some similarity in molecular structure compared to endogenous estrogens and bind to some extent with estrogen receptors. Phytoestrogens have been shown to affect mammalian physiology including cancer growth, atherosclerosis, calcium-phosphorus metabolism, and behavior. This is of concern to researchers because standard natural ingredient diets are often composed of ingredients which contain significant quantities of phytoestrogens. Soybean meal is an ingredient common to standard natural ingredient diets which contains a class of phytoestrogens called isoflavones. The two primary isoflavones are genistein and daidzein. Alfalfa meal is a second ingredient common to standard natural ingredient diets. It contains the phytoestrogen coumestrol.

Custom research diets

COLLAPSE ALL TABS

Custom research diets are made in small quantities and are generally not stocked. These diets are typically formulated for a specific type of research objective. These diets can consist of refined ingredients (purified diets), natural ingredients (grain and other ingredients found in standard diets), or utilize a standard diet as a base to which other ingredients are added. Any of these diet types can serve as the basis to add a customer supplied ingredient (test article, pharmaceutical, food extract, etc). We have approximately 20,000 diets in our database, so the formula you require may already have been created. We also utilize this database as a resource when formulating new diets. We will be pleased to provide you a complete formula sheet, containing the ingredients and inclusion rate, macronutrient values, key features, and planning and ordering information.

Purified diets use refined ingredients such as casein, sucrose, cornstarch, and cellulose. These human food grade ingredients have relatively simple chemical compositions (predominantly one nutrient classification) and this feature is important for manipulating individual nutrients for research purposes. Additionally, most refined ingredients contain very limited levels of non-nutrients that could have biological activity. This is in contrast to the natural ingredients (corn, wheat, soybean meal, etc.) used in standard diets, which have relatively complex chemical compositions as well as various phytochemicals that may or may not be physiologically relevant. Refined ingredients are obtained from reliable suppliers of human grade products, and we consistently obtain ingredients from the same vendors, year after year. Additionally, the major protein ingredients are assayed for a specific panel of nutrients to ensure minimal variation. Thus, purified diets will be repeatable and can easily be modified. Examples of common purified diets include AIN-76A (CA.170481), AIN-93G (TD.94045), and AIN-93M (TD.94048).

The American Society for Nutrition (ASN) (formerly American Institute of Nutrition, AIN) has promoted the use of nutritionally adequate purified diets for rodents, because many researchers may not be aware of potential nutritional variables. The AIN-76 formula was published in 1977, and became AIN-76A with minor revision (increased Vitamin K) in 1980. The diet has been used extensively, and there were several suggestions about improvements. This resulted in the publication of two formulas in 1993, AIN-93G (for growth, reproduction, and lactation) and AIN-93M (for maintenance). The article J. Nutr. 123: 1939-1951 (1993) discusses the rationale for the several revisions. Some of the changes include soybean oil rather than corn oil, the addition of other carbohydrate sources and lower sucrose, decreased phosphorus, cystine in place of methionine, inclusion of several trace mineral elements, and a few vitamin adjustments. The maintenance diet has less protein and fat.

Each formula is given a unique identification number and retains that identity among the thousands of formulas in our files. This assures the same formula will be used even years later. Upon production, a six or seven-digit “Rx” lot number is assigned that allows for traceability through all phases of diet production. These detailed production records are kept for every item produced. Quality-control samples are retained for nine months. Each lot of the major protein ingredients is analyzed for a number of nutrients to verify uniformity from lot to lot. Care is taken to retain reputable vendors for these refined ingredients.

Yes, we can help you in adding a test compound or ingredient to a diet. This is often a convenient approach to dosing research animals. When adding a compound to a diet, there are some important safety and stability considerations. Please see customer supplied ingredients for more specific information.

A phone call or an email exchange with one of our staff will augment the information that you gather from the web site or published literature, and you are more likely to receive a diet that best meets your particular needs. We are careful to maintain confidentiality, and will sign formal agreements if necessary. Please contact us at info@jinpanbio.com for consultation regarding your specific diet needs.

Advise us why the diet is needed, and for what animal species. Discuss the nutrients or ingredients of interest in as much detail as possible. Please avoid subjective descriptions such as “low, normal, and high” because these terms have different meanings to various researchers. Provide us with a copy of any existing formula to be duplicated or modified, and any journal article(s) upon which your research may be based. Let us know if the diet must be irradiated.

Our minimum order for custom research diets is 3 kg. This is particularly convenient when feeding small numbers of mice, especially for diet that contains your test compound. The minimum quantity for vitamin and mineral mixes is 500 g.

我们的饮食风俗研究最少是 3 公斤。这是特别方便的小数量喂养、饮食含有测试化合物。在最小量的维生素和矿物质混合料是 500 克。

Prices are dependent on formula details, ingredient composition, order quantity, and form (pellet or powder). If you know the product code, contact customer service at  info@jinpanbio.com. If you are unsure of the product code, have a new request, or require other technical information, contact a Nutritionist info@jinpanbio.com.

Standard packaging includes a small box (nine x nine x 11 inches) that will fit up to five kg, a medium box (ten x ten x 15 inches) that will fit up to ten kg, and a larger heavy-duty box (13 x 13 x 20 inches) with inset handles that holds up to 20 kg. Every container also includes a three-mil poly liner to keep your research diet as fresh as possible. Vacuum packaging and specific quantity packaging are also available at additional cost. Vacuum packaging will typically reduce the amount of diet fit into a box. Contact us at info@jinpanbio.com for more information.

Diets are custom made to fill each order. The typical lead-time between order and shipment is ten business days. The exceptions to this are TD.88137, TD.06414, and TD.01306 which for orders of 25 kg or less, are usually available within a week. If irradiation is required (add ten business days). We can often accommodate your rush production needs, for an additional fee. Contact us at info@jinpanbio.com for more details. Transit time depends on the type of delivery method used (regular ground, overnight, etc.). Weight and delivery method affect shipping costs, which are typically prepaid and added to the invoice.

There is no definitive point where one is able to predict when a specific diet will spoil or become deficient in one or more nutrients. Custom research diets are perishable and should be refrigerated at 4 degrees celsius (40 degrees fahrenheit) or lower (see Fullerton, et.al. J Nutr. 112:567-573, 1982). As a general guideline, diets can be stored this way for up to six months. Freezing a diet may slow reactive processes, but there is not sufficient information available to recommend feeding a diet past six months as a general guideline. Use of diets beyond the recommended use date will be subject to the protocols of your institution.

没有一个人能够预测当特定的饲料会破坏或缺乏一个或更多的营养。饲料习俗研究很容易腐烂并冷藏在 4 ℃ 或更低 (参见 Fullerton 等。J Nutr 。112 : 567 – 573 , 1982) 。作为一般性指导 , 可这种方法存放六个月之久。冷冻的食物可减慢反应过程 , 但是没有足够的信息可用以推荐的饮食喂养 6 个月。使用饲料建议的使用日期的协议。

Custom research diets vary significantly in their formulation. There may be certain features of a diet that merit a shorter shelf-life. To learn more about storage and use recommendations, and to help you asess if your diet has features that may warrant a shorter shelf-life we have put together a guide. If you have further questions about your diet, contact us at info@jinpanbio.com.

Barrier facilities or certain animal models require sterilized diet. Most custom research diets do not withstand autoclaving; however, we can arrange for qualified diets to be irradiated. The irradiation dose range is two to five MRad, or 20-50 kGy. Nominal fees and extra time are involved. Please contact us at info@jinpanbio.com for details about irradiation costs and additional lead-time.

A nutritionist can assist you in determining if a formula is suitable for irradiation. Irradiation will lead to selective vitamin loss, and it is our practice to increase the inclusion rate of certain vitamin mixes to help ensure that the final diet contains sufficient vitamins. Irradiated diets are double-bagged. Vacuum packaging may be helpful in reducing irradiation-induced peroxide formation in high fat diets.

Some diet formulas are fairly common or generic, and probably are not considered confidential by those who use them, especially when authors provide a product number. Formulas might be difficult to trace without a product number, and then must be discussed with a nutritionist. We will review each formula for history and confidentiality.

Provide us with at the very least a description, preferably the complete formula, and we’ll give you an assessment of our ability to make that formula.

There are limitations for pelleting diets. The level and type of fat, the carbohydrate composition, and the interaction of fat and carbohydrate will impact our ability to pellet a diet. Consult a nutritionist at info@jinpanbio.com for more information about specific formulas.

“Vitamin-Free” Test Casein is an alcohol-extracted casein prepared from regular casein, and typically has 90-91% protein (%N x 6.38), 0.1% fat, and four-six % moisture. The alcohol extraction reduces fat, fat soluble vitamins, and some of the B vitamins. This protein source is most appropriately used in vitamin deficient diets, and when researchers want to minimize “background fat” in a diet. We have prepared this specialty ingredient for many years, and continue to supply it to many labs. We will develop special pricing on request for large quantities. Advance notice is recommended for orders of 1,000 Kg or more. If you need this ingredient, consider Envigo as your preferred source.

Yes. Some of the same high-quality ingredients used to produce custom research diets can also be supplied to those who prepare their own diets. See diet ingredients for more information.

When citing Teklad diets, include a description, and a product number — standard grain based diets are typically four numbers while custom diets usually start with “TD” followed by five or six numbers. Use the following examples as a guide:

“Rats were maintained on minimal phytoestrogen diet 2016 Teklad Global 16% Protein Rodent Diet, Envigo, Madison, Wisconsin USA, www.amrescoinc.cn”

“Obesity was induced by feeding Teklad high fat diet TD.06414 with 60% of kcal from fat, primarily lard, Envigo, Madison, Wisconsin USA, www.amrescoinc.cn”

“Mice were fed Teklad vitamin D deficient diet TD.89123, Envigo, Madison Wisconsin USA, www.amrescoinc.cn

Each diet has a unique product number that will allow others to quickly and easily obtain information about the diet used in your studies by contacting info@jinpanbio.com

No. According to the NIH OACU Guidelines for Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals (2008), the vehicle used to facilitate administration of a compound is as important of a consideration as the active compound in the preparation. Diet as a vehicle, either whole or as the sum of its parts, would not meet the standard for pharmaceutical grade as defined by this document.

You may have been asked this question by your IACUC due to a passage in the 8thedition of the Guide for the Care and Use of Laboratory Animals (2011) stating that pharmaceutical-grade chemicals and other substances should be used, when available, for all animal-related procedures (page 31). In general, deviations from “Must” or “Should” statements within the Guide require justification and approval by your institutional IACUC.

Alternative dosing techniques (gavage, injection) can introduce stress from handling and increase the risk for potential injury. Stress can affect animal well-being as well as scientific parameters through modifications in behavior and physiology. Dosing via diet is an established method for delivering test compounds to animals that is non-invasive, reducing stress and potential injury from handling. Therefore, use of non-pharmaceutical grade compounds as a delivery method is justified, as it is advantageous for both animal welfare and experimental design.

Further reading:

Guide for the Care and Use of Laboratory Animals: 8th Edition (2011). National Research Council Committee for the Update of the Guide for the Care and Use of Laboratory Animals.

Guidelines for the Use of Non-Pharmaceutical-Grade Chemicals/Compounds in Laboratory Animals. (2008). Animal Research Advisory Committee, Office of Animal Care and Use, NIH.

Laboratory routines cause animal stress. (2004). Balcombe, J.P., Barnard, N.D., and Sandusky, C. Contemp Top Lab Anim Sci 43, 42-51.

Harlan Teklad动物饲料目录以及Harlan饲料价格

Harlan Teklad动物饲料目录以及Harlan饲料价格

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad动物饲料目录以及Harlan饲料价格

TD.94045 AIN-93G Purified Diet AIN-93G纯化饮食
TD.94048 AIN-93M Purified Diet AIN-93M纯化饮食
TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased) 适用于辐射的AIN-76A型(维生素水平增加)
TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased) 适用于辐照的AIN-93G版本(维生素水平增加)
TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased) 适用于辐照的AIN-93M版本(增加维生素水平)
TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil. 用玉米油代替豆油的AIN-93G的改性。
TD.06414 Adjusted Calories Diet (60/Fat) 调整后的卡路里饮食(60 /脂肪)
TD.06415 Adjusted Calories Diet (45/Fat) 调整后的卡路里饮食(45 /脂肪)
TD.03584 35% Lard Diet (Adj., No C) 35%猪油饮食(调整,否C)
TD.93075 Adjusted Calories Diet (55/fat) 调整后的卡路里饮食(55 /脂肪)
TD.95217 Adjusted Fat Diet 调整脂肪饮食
TD.96132 Adjusted Fat Diet 调整脂肪饮食
TD.88137 Adjusted Kcal Diet (42% from fat 调整后的Kcal饮食(42%来自脂肪
TD.08811 45% Kcal Fat Diet (21% MF, 2% SBO) 45%Kcal脂肪饮食(21%MF,2%SBO)
TD.06416 Adjusted Calories Diet (10/Fat) (a possible control for TD.06415 & TD.06414 listed above) 调整后的卡路里饮食(10 /脂肪)(上面列出的TD.06415和TD.06414的可能控制)
TD.08810 Low Glycemic Control Diet 低血糖控制饮食
TD.96355 Ketogenic Diet 生酮饮食
TD.84224 Essential Fatty Acid Deficient Diet 必需脂肪酸缺乏饮食
TD.88137 Adjusted Calories Diet (42% from fat) 调整后的卡路里饮食(42%来自脂肪)
TD.88051 Cocoa Butter and Purina Chow Diet 可可脂和Purina Chow饮食
TD.90221 Cocoa Butter Diet with 75% Purina Mouse (5015) 含75%Purina小鼠的可可脂饮食(5015)
TD.94059 5015, Cocoa Butter, Chol., etc. (excludes cholate) 5015,可可脂,Chol。等(不包括胆酸盐)
TD.02028 Atherogenic Rodent Diet (1.25% Chol. & 0.5% Cholic Acid) 动脉粥样硬化啮齿动物饮食(1.25%Chol。和0.5%胆酸)
TD.01383 2018 + 2% Cholesterol 2018 + 2%胆固醇
TD.86143 Vitamin A Deficient Diet 维生素A缺乏饮食
TD.89123 Vitamin D Deficient Diet 维生素D缺乏饮食
TD.88163 Vitamin E Deficient Diet 维生素E缺乏饮食
TD.95247 Folic Acid Deficient Diet 叶酸缺乏饮食
TD.80396 Iron Deficient Diet 缺铁饮食
TD.85419 Zinc Deficient Diet 锌缺乏饮食
TD.88239 Potassium Deficient Diet 缺钾饮食
TD.95027 Low Calcium Diet (0.4% P) 低钙饮食(0.4%P)
TD.96348 Diet (20% Lactose, 2% Ca, 1.25% P) 饮食(20%乳糖,2%Ca,1.25%P)
TD.92163 Selenium Deficient Diet 硒缺乏饮食
TD.95125 Iodine Deficient (0.15% PTU) 碘缺乏(0.15%PTU)
TD.93328 Protein Free Diet 无蛋白质饮食
TD.90016 6% Protein Diet 6%蛋白质饮食
TD.91352 20% Protein Diet 20%蛋白质饮食
TD.90018 40% Protein Diet 40%蛋白质饮食
TD.89247 60% Fructose Diet 60%果糖饮食
TD.86489 Diet with Adjusted Sucrose/Cornstarch 调整蔗糖/玉米淀粉的饮食
TD.96348 Diet (20% Lactose, 2% Ca, 1.25% P) 饮食(20%乳糖,2%Ca,1.25%P)
TD.98090 70% Carbohydrate Diet 70%碳水化合物饮食
TD.96355 Ketogenic Diet (almost no carbohydrate) 生酮饮食(几乎不含碳水化合物)
TD.99366 Amino Acid Diet 氨基酸饮食
TD.90262 Methionine & Choline Deficient Diet 蛋氨酸和胆碱缺乏饮食
TD.00434 Folic Acid Deficient Diet 叶酸缺乏饮食
TD.99386 No Lysine AA Diet 没有赖氨酸AA饮食
TD.90228 Sodium Deficient Diet 钠缺乏饮食
TD.96208 0.49% NaCl Diet 0.49%NaCl饮食
TD.90229 1% NaCl Diet 1%NaCl饮食
TD.92034 4% NaCl Diet 4%NaCl饮食
TD.92012 8% NaCl Diet 8%NaCl饮食
TD.01306 Rodent Diet (2018 + 625 doxycycline) 啮齿动物饮食(2018 + 625强力霉素)
TD.98186 Rodent Diet (7012 + 200 doxycycline) 啮齿动物饮食(7012 + 200强力霉素)
TD.92033 Swine Diet (15% Lard, 1.2% Cholesterol) 猪日粮(15%猪油,1.2%胆固醇)
TD.92052 Swine Diet (15% Lard, 1.5% Cholesterol) 猪日粮(15%猪油,1.5%胆固醇)
TD.93296 Swine Diet (15% Lard, 2% Cholesterol) 猪日粮(15%猪油,2%胆固醇)
CA.40060 Teklad Vitamin Mix Teklad维生素混合物
CA.40077 AIN-76A Vitamin Mix AIN-76A维生素混合物
TD.94047 AIN-93 Vitamin Mix AIN-93维生素混合物
CA.170915 AIN-76 Mineral Mix AIN-76矿物质混合物
TD.94046 AIN-93G Mineral Mix AIN-93G矿物质混合物
TD.94049 AIN-93M Mineral Mix AIN-93M矿物质混合物
TD.79055 Calcium & Phosphorus Deficient Mineral Mixed based on AIN-76 基于AIN-76的钙磷缺乏矿物混合物
TD.98057 Calcium & Phosphorus Deficient Mineral Mixed based on AIN-93 基于AIN-93的钙磷缺乏矿物混合物

Harlan TD.95027低钙饲料(0.4%P)

Harlan TD.95027低钙饲料(0.4%P)

Harlan货号:TD.95027

英文名称:Low Calcium Diet (0.4% P)

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula                                                                     g/Kg

Casein                                                                    200.0

Cystine                                                                     3.0

Sucrose                                                            342.188

Corn Starch                                                         320.0

Soybean Oil                                                          60.0

Cellulose                                                              40.0

Mineral Mix, Ca-P Deficient (79055)                    13.37

Potassium Phosphate, monobasic                     11.43

Vitamin Mix, Teklad (40060)                                10.0

Ethoxyquin, antioxidant                                       0.012

Key Features

+ Purified Diet

+ Calcium

+ Phosphorus

Key Planning Information

+ Products are made fresh to order

+Store product at 4°C or lower

+Use within 6 months (applicable to most diets)

+Box labeled with product name, manufacturing date, and lot number
+ Replace diet at minimum once per week More frequent replacement may be advised
+ Lead time:
· 2 weeks non-irradiated
· 4 weeks irradiated

上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫诊断试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

上海金畔生物科技有限公司

服务热线:18301939375   QQ:3258089810    3259632176

Email:  info@jinpanbio.com

官网:http://www.jinpanbio.com

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan 动脉粥样硬化(胆固醇、脂肪、胆酸盐) 饲料Atherogenic

Atherogenic

Research use Key dietary features Examples
“Western” purified atherogenic diet
Accelerated hypercholesterolemia and plaque formation in genetically modified models such as Apoe and Ldlr deficient mice.

Used for diet induced obesity in a variety of rodent models.

High fat diet (20 – 23% by weight; 40 – 45% kcal from fat)

Saturated fatty acids (SFA >60% of total fatty acids)

Milkfat/butterfat

Sucrose (34% by weight)

Cholesterol (0.2% total)

TD.88137
TD.10885
“Western” purified atherogenic diet with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

High fat diet (15 – 20% by weight; 34 – 45% kcal from fat)

Saturated fatty acids (SFA >55% of total fatty acids)

Milkfat/butterfat, cocoa butter

Sucrose (30-50% by weight)

Cholesterol (1 – 1.25%)

Cholate Source (0.5%)*

TD.02028
TD.09237
Hybrid high fat diets with added cholesterol and cholate source*
Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) in primarily wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

75% rodent breeder diet; 25% purified ingredients

High fat (~15% by weight; 37% kcal from fat)

Saturated fatty acids (SFA >45% of total fatty acids)

Cholesterol (1.25%)

Cholate source (0.5%)*

TD.88051
TD.90221
Standard diets with added cholesterol
Induce hypercholesterolemia in genetically modified and wild type models without promoting obesity. Standard, grain-based rodent diet

Minimal/moderate phytoestrogen diets recommended

Cholesterol (1 – 4%)

TD.120097
TD.07841
TD.01383

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis. However, if including a cholate source is not desired for your research, diets without cholate are available.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.

在实验动物模型中,饮食是诱导或加速动脉粥样硬化的有用工具。用于诱发啮齿类动物动脉粥样硬化的主要饮食特征因研究模型、所需终点和喂养时间的不同而不同。尽管动脉粥样硬化饮食的配方在不断发展,但文献中详细描述的备选方案概述如下。有关每个饮食选项和文献参考的更多信息,请参见节目表后面的可扩展选项卡。

研究用途 主要饮食特征 实例
“西方”纯正致动脉粥样硬化饮食
加速高胆固醇血症和斑块形成的转基因模型,如APOE和Ldlr缺乏的小鼠。

用于饮食诱导肥胖的各种啮齿动物模型。

  • 高脂饮食(按体重计算占20-23%;脂肪占40-45%)
  • 饱和脂肪酸(SFA>总脂肪酸的60%)
  • 乳脂/乳脂
  • 蔗糖(按重量计占34%)
  • 胆固醇(总数0.2%)
TD.88137
TD.10885
添加胆固醇和胆酸来源的“西方”纯动脉粥样硬化饮食*
在野生型小鼠和大鼠中诱导高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

  • 高脂饮食(体重15-20%;脂肪34-45千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的55%)
  • 乳脂/黄油、可可脂
  • 蔗糖(重量占30%-50%)
  • 胆固醇(1-1.25%)
  • 巧克力来源(0.5%)*
TD.02028
TD.09237
添加胆固醇和胆酸盐的混合高脂饮食*
在野生型小鼠和大鼠中诱导高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

也用于造石(胆石)啮齿动物的研究。

  • 75%的啮齿动物饲养员;25%的纯化成分
  • 高脂肪(体重约15%;脂肪37%千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的45%)
  • 胆固醇(1.25%)
  • 巧克力源(0.5%)*
TD.88051
TD.90221
添加胆固醇的标准饮食
在转基因和野生型模型中诱导高胆固醇血症而不促进肥胖。
  • 标准的、以谷物为基础的啮齿动物饮食
  • 最小/中度植物雌激素饮食建议
  • 胆固醇(1-4%)
TD.120097
TD.07841
TD.01383

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。

“Western” purified atherogenic diet

“Western” style diets are fed to genetically-modified cardiovascular models, such as Apoe and Ldlr deficient mice, to accelerate and enhance hypercholesterolemia and plaque formation and to elicit phenotypes commonly associated with metabolic syndrome.Within the atherogenic literature, a “Western” diet typically is described as a purified rodent diet with 20-23% milkfat/butterfat, 0.2% total cholesterol, and 34% sucrose by weight.TD.88137is an example of a “Western” style diet that was originally designed to characterize and enhance atherosclerosis development in a newly generated Apoe-deficient mouse model.Contact us for more information about “Western” style diets, modifications, or possible control diets.

Examples:

TD.88137Adjusted calories diet (42% from fat, 0.2% total cholesterol)

TD.1088545% fat Kcal diet (0.2% total cholesterol)

Research use:

Accelerated hypercholesterolemia and plaque formation in genetically-modified models, such as Apoe and Ldlr deficient mice.

Used for diet-induced obesity in a variety of rodent models.

Key dietary features:

High Fat Diet (20-23% by weight; 40 – 45% kcal from fat)

Saturated fatty acids (SFA >60% of total fatty acids)

Milkfat/butterfat

Sucrose (34% by weight)

Cholesterol (0.2% total)

References:

1.Febbraio, M., et al., Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice.J Clin Invest, 2000.105(8): p.1049-56.

2.Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptor-deficient mice with attenuated expression of scavenger receptor B1.Arterioscler Thromb Vasc Biol, 2000.20(4): p.1068-73.

3.Nakashima, Y., et al., ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree.Arterioscler Thromb, 1994.14(1): p.133-40.

4.Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone sites on the endothelium in the ApoE-deficient mouse.Arterioscler Thromb Vasc Biol, 1998.18(5): p.842-51.

5.Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells.Cell, 1992.71(2): p.343-53.

6.Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory program in the aortas of low density lipoprotein receptor-deficient mice.J Biol Chem, 1998.273(46): p.30427-34.

7,Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular endothelium to protect mice from atherosclerosis.Cell Metab, 2012.15(3): p.372-81.

“Western” purified atherogenic diet with added cholesterol and cholate source*

Wild type mice and rats generally are resistant to atherosclerosis, requiring more extreme dietary manipulation to modify lipoprotein profiles and develop mild atherosclerosis (foam cells, fatty streaks).Modern formulations are made completely of purified ingredients because this more refined approach has been reported to decrease the incidence of gallstones and liver damage associated with less refined and more traditional dietary approaches.To induce mild atherosclerosis in wild type animals, the “Western” purified diet can be modified to increase cholesterol (1-1.25%) and add a bile salt such as sodium cholate or cholic acid.Contact us for more information, modifications, or possible control diets.

Examples of purified high fat diets with added cholesterol and cholate source*:

TD.0202821% milkfat (1.25% cholesterol, 0.5% cholic acid)

TD.0923715% milkfat diet (1% cholesterol, 0.5% sodium cholate)

Examples of purified high fat diets with added cholesterol (without cholate source):

TD.9612121% milkfat (1.25% cholesterol)

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Key dietary features:

High fat diet (15-20% by weight; 34 – 45% kcal from fat)

Saturated fatty acids (SFA >55% of total fatty acids)

Milkfat/butterfat, cocoa butter

Sucrose (30-50% by weight)

Cholesterol (1 – 1.25%)

Cholate source (0.5%)*

References:

1.Bernal, C., et al., Lipid biomarkers and metabolic effects of lycopene from tomato juice on liver of rats with induced hepatic steatosis.J Nutr Biochem, 2013.24(11): p.1870-81.

2.Gao, Q., et al., Atherogenic diets exacerbate colitis in mice deficient in glutathione peroxidase.Inflamm Bowel Dis, 2010.16(12): p.2043-54.

3.Lichtman, A.H., et al., Hyperlipidemia and atherosclerotic lesion development in LDL receptor-deficient mice fed defined semipurified diets with and without cholate.Arterioscler Thromb Vasc Biol, 1999.19(8): p.1938-44.

4.Marcondes, M.C., et al., Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta.Brain Behav Immun, 2011.25(8): p.1649-57.

5.Nishina, P.M., et al., Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice.J Lipid Res, 1993.34(8): p.1413-22.

6.Nishina, P.M., et al., Atherosclerosis and plasma and liver lipids in nine inbred strains of mice.Lipids, 1993.28(7): p.599-605.

7.Yue, P., et al., Enhanced hepatic apoA-I secretion and peripheral efflux of cholesterol and phospholipid in CD36 null mice.PLoS One, 2010.5(3): p.e9906.

8.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.

Hybrid high fat diets with added cholesterol and cholate source*

Beverly Paigen and colleagues first characterized atherosclerosis development in C57BL/6 mice by feeding a hybrid atherogenic diet.The hybrid diet was created by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate; referred to as Thomas-Hartroft diet).The resulting mixture recreated inTD.88051/TD.90221(same formula) contains ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate.This group later compared the hybrid atherogenic diet approach to the more modern “western” purified atherogenic diet with added cholesterol and cholate and found that the hybrid atherogenic diet induced more gallstones and liver damage.Hybrid diets contain a variety of unrefined ingredients that may modify lipid metabolism and atherogenesis and do not allow for precise control of ingredients and nutrients for the study of chronic diseases.Although more refined diets have been developed, hybrid atherogenic diets are still popular for inducing mild atherosclerosis and gallstones in wild type mice and rats.Contact us for more information, modifications, or possible control diets.

Examples of hybrid high-fat diets with added cholesterol and cholate source*:

TD.88051andTD.90221(same formula) are Teklad product codes for hybrid atherogenic diets

Example of hybrid high-fat diet with added cholesterol (without cholate source):

TD.94059

Research Use:

Induce hypercholesterolemia and mild atherosclerosis (foam cells, fatty streaks) primarily in wild type mice and rats.

Will not promote obesity.

Also used for lithogenic (gallstone) rodent studies.

Key dietary features:

75% rodent breeder diet; 25% purified ingredients

High fat (~15% by weight; 37% kcal from fat)

Saturated fatty acids (SFA >45% of total fatty acids)

Cholesterol (1.25%)

Cholate source (0.5%)*

References:

1.Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse.J Lipid Res, 1990.31(5): p.859-69.

2.Clee, S.M., et al., Plasma and vessel wall lipoprotein lipase have different roles in atherosclerosis.J Lipid Res, 2000.41(4): p.521-31.

3.George, J., et al., Enhanced fatty streak formation in C57BL/6J mice by immunization with heat shock protein-65.Arterioscler Thromb Vasc Biol, 1999.19(3): p.505-10.

4.Miyake, J.H., et al., Transgenic expression of cholesterol-7-alpha-hydroxylase prevents atherosclerosis in C57BL/6J mice.Arterioscler Thromb Vasc Biol, 2002.22(1): p.121-6.

5.Paigen, B., et al., Quantitative assessment of atherosclerotic lesions in mice.Atherosclerosis, 1987.68(3): p.231-40.

6.Schreyer, S.A., D.L. Wilson, and R.C. LeBoeuf, C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis.Atherosclerosis, 1998.136(1): p.17-24.

7.Vergnes, L., et al., Cholesterol and cholate components of an atherogenic diet induce distinct stages of hepatic inflammatory gene expression.J Biol Chem, 2003.278(44): p.42774-84.

*Sodium cholate or cholic acid aid cholesterol and fat absorption and reduce cholesterol disposal via bile acid synthesis.However, if including a cholate source is not desired for your research, diets without cholate are available.SeeTD.96121for a purified diet andTD.94059for a hybrid diet.Contact us for additional options.

Standard diets with added cholesterol

Standard, natural ingredient diets with cholesterol added are fed to induce hypercholesterolemia.Various levels of cholesterol, fat, and/or bile salts can be added to one of the numerous standard rodent diets stocked by Envigo Teklad.For many applications, adding these components to Envigo’s minimal-to-moderate phytoestrogen global rodent diets is recommended.Our minimal phytoestrogen global rodent diets are soybean meal free, limiting the effect of phytoestrogens on your research outcomes.Soybean meal, a common dietary source of phytoestrogens, has been shown to decrease aortic fatty streak development and modify plasma cholesterol, which may reduce the risk of developing atherosclerosis.Limiting dietary soybean meal may reduce confounding variables within your dietary-induced atherosclerosis model.Contact a nutritionist to discuss additional diet options.

Examples of minimal and moderate phytoestrogen rodent diets with added cholesterol:

TD.1200971% cholesterol diet (2020 – minimal phytoestrogens)

TD.078412% cholesterol diet (2016 – minimal phytoestrogens)

TD.013832% cholesterol (2018 – Moderate phytoestrogens)

Research use:

Induce hypercholesterolemia in genetically-modified and wild type models without promoting obesity.

Key dietary features:

Standard, grain-based rodent diet

Minimal/moderate phytoestrogen diets recommended

Cholesterol (1 – 4%)

References:

1.Belch, J.J., et al., Longitudinal assessment of endothelial function in the microvasculature of mice in-vivo.Microvasc Res, 2013.85: p.86-92.

2.Hartvigsen, K., et al., A diet-induced hypercholesterolemic murine model to study atherogenesis without obesity and metabolic syndrome.Arterioscler Thromb Vasc Biol, 2007.27(4): p.878-85.

Diets for additional animal models of atherosclerosis

Rabbits, hamsters, and swine are common models of atherosclerosis.Contact a nutritionist for information and formula examples.Seerabbit, swine and other speciesfor information and formula examples.

“西方”纯正致动脉粥样硬化饮食

“西式”饮食被喂给基因修饰的心血管模型,如APOE和Ldlr缺乏的小鼠,以加速和促进高胆固醇血症和斑块形成,并引出通常与代谢综合征相关的表型。在动脉粥样硬化的文献中,“西方”饮食通常被描述为含有20-23%乳脂/蝴蝶脂肪、0.2%总胆固醇和34%蔗糖的纯啮齿动物饮食。TD.88137这是一种“西式”饮食的例子,最初的目的是在一种新生成的APOE缺陷小鼠模型中描述和促进动脉粥样硬化的发展。联系我们,以获得更多关于“西方”风格的饮食,修改,或可能的控制饮食。

例子:

  • TD.88137调整卡路里饮食(42%来自脂肪,0.2%总胆固醇)
  • TD.1088545%脂肪KCAL饮食(总胆固醇0.2%)

研究用途:

加速高胆固醇血症和斑块形成的转基因模型,如APOE和Ldlr缺乏的小鼠。

用于饮食诱导肥胖的各种啮齿动物模型。

主要饮食特征:

  • 高脂肪饮食(按体重计算占20-23%;脂肪占40-45%)
  • 饱和脂肪酸(SFA>总脂肪酸的60%)
  • 乳脂/乳脂
  • 蔗糖(按重量计占34%)
  • 胆固醇(总数0.2%)

野生型小鼠和大鼠一般对动脉粥样硬化有抵抗力,需要更极端的饮食操作来改变脂蛋白谱,形成轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。现代配方完全是由纯化成分制成的,因为据报道,这种更精细的方法可以减少较少精制和较传统饮食方法引起的胆结石和肝脏损害的发生率。为了在野生型动物中诱导轻度动脉粥样硬化,可对“西方”纯化饲料进行改良,以提高胆固醇(1-1.25%),并添加胆酸钠或胆酸等胆盐。联系我们获得更多的信息,修改,或可能的控制饮食。

添加胆固醇和胆酸盐来源的纯化高脂饮食的例子*:

  • TD.0202821%乳脂(1.25%胆固醇,0.5%胆酸)
  • TD.0923715%乳脂饮食(1%胆固醇,0.5%胆酸钠)

添加胆固醇的纯化高脂饮食的例子(没有胆酸来源):

  • TD.9612121%乳脂(1.25%胆固醇)

研究用途:

主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

主要饮食特征:

  • 高脂饮食(体重15-20%;脂肪34-45千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的55%)
  • 乳脂/黄油、可可脂
  • 蔗糖(重量占30%-50%)
  • 胆固醇(1-1.25%)
  • 巧克力源(0.5%)*

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系,了解其他选项。

贝弗利·派根和他的同事首先通过喂养一种混合性动脉粥样硬化饮食来描述C57BL/6小鼠动脉粥样硬化的发展。这种混合饲料是通过将一种天然成分的老鼠饲料按3:1的比例与浓缩的纯化饲料(含5%胆固醇和2%胆酸钠;称为Thoms-Hartroft饮食)混合而成的。产生的混合物在TD.88051/TD.90221(同配方)含有~15.8%脂肪、1.25%胆固醇和0.5%胆酸钠。这组人后来将混合动脉粥样硬化饮食方法与更现代的“西方”纯化动脉粥样硬化饮食方法进行了比较,并添加了胆固醇和胆酸盐,发现混合动脉粥样硬化饮食会导致更多的胆结石和肝脏损伤。混合饮食含有多种未精制的成分,这些成分可能会改变血脂代谢和动脉粥样硬化的发生,并且不允许对成分和营养物进行精确控制,以用于慢性病的研究。虽然已经开发出了更精细的饮食,但混合动脉粥样硬化饮食仍然是野生型小鼠和大鼠诱发轻度动脉粥样硬化和胆结石的流行食品。联系我们获得更多的信息,修改,或可能的控制饮食。

添加胆固醇和胆酸盐的混合高脂饮食实例*:

  • TD.88051和TD.90221(相同的配方)是特克拉德混合动脉粥样硬化饮食的产品代码。

添加胆固醇的混合高脂饮食的例子(没有胆酸来源):

  • TD.94059

研究用途:

主要在野生型小鼠和大鼠诱发高胆固醇血症和轻度动脉粥样硬化(泡沫细胞、脂肪条纹)。

不会助长肥胖。

也用于造石(胆石)啮齿动物的研究。

主要饮食特征:

  • 75%的啮齿动物饲养员;25%的纯化成分
  • 高脂肪(体重约15%;脂肪37%千卡)
  • 饱和脂肪酸(SFA>总脂肪酸的45%)
  • 胆固醇(1.25%)
  • 巧克力源(0.5%)*

*胆酸钠或胆酸有助胆固醇和脂肪的吸收,并可透过胆汁酸合成减少胆固醇的排出。然而,如果你的研究不想包括胆酸盐的来源,没有胆酸盐的饮食是可用的。看见TD.96121为了纯正的饮食TD.94059混合饮食。请与我们联系,了解其他选项。

标准的,添加胆固醇的天然成分饮食是用来诱导高胆固醇血症的。不同水平的胆固醇,脂肪和/或胆汁酸盐可以添加到众多的标准啮齿动物饮食之一,由EnvigoTeclad。在许多应用中,建议将这些成分添加到Envigo的最小到中等植物雌激素的全球啮齿动物饮食中。我们最小的植物雌激素全球啮齿动物饮食是不含豆粕的,限制了植物雌激素对你的研究结果的影响。豆粕是一种常见的植物雌激素来源,它可以减少主动脉脂肪条纹的发育,改善血浆胆固醇,从而降低动脉粥样硬化的风险。限制饮食豆粕可能会减少饮食诱导动脉粥样硬化模型中的混杂变量。联系营养学家讨论其他饮食选择。

添加胆固醇的最小和中度植物雌激素啮齿动物饮食的例子:

  • TD.120097  1%胆固醇饮食(2020年-最小植物雌激素)
  • TD.07841  2%胆固醇饮食(2016年-最小植物雌激素)
  • TD.01383  2%胆固醇(2018年-中度植物雌激素)

研究用途:

在转基因和野生型模型中诱导高胆固醇血症而不促进肥胖。

主要饮食特征:

  • 标准的、以谷物为基础的啮齿动物饮食
  • 最小/中度植物雌激素饮食建议
  • 胆固醇(1-4%)

兔子、仓鼠和猪是动脉粥样硬化的常见模型。请与营养师联系,了解有关信息和公式示例。看见兔子、猪和其他物种有关信息和公式示例。