BD Pharmingen 555899 Lysing Buffer 红细胞裂解液- BD 349202 FACS Lysing Solution 区别

BD Pharmingen 555899 Lysing Buffer 红细胞裂解液- BD 349202 FACS Lysing Solution 区别

BD 555899 Lysing Buffer 红细胞裂解液

产品品牌:BD

产品产地:美国

产品规格:100ml

用途范围:流式细胞术(常规检测)

描述

BD Pharm Lyse™ lysing solution is a buffered, concentrated (10X) ammonium chloride-based lysing reagent. When diluted to a 1X concentration and used as recommended, BD Pharm Lyse™ lyses red blood cells following monoclonal antibody staining. The lysing solution results in good light scatter separation of lymphocytes and red blood cell debris when analyzed by flow cytometry.  BD Pharm Lyse™ does not contain a fixative agent, so leukocytes remain viable after red blood cell lysis.

BD Pharm Lyse™
RUO
AB_2869057
Flow cytometry (Routinely Tested)

BD Pharm Lyse™ 裂解液是一种缓冲、浓缩 (10X) 氯化铵基裂解试剂。 当稀释至 1X 浓度并按照推荐使用时,BD Pharm Lyse™ 会在单克隆抗体染色后裂解红细胞。 当通过流式细胞仪分析时,裂解溶液导致淋巴细胞和红细胞碎片的良好光散射分离。 BD Pharm Lyse™ 不含固定剂,因此白细胞在红细胞裂解后仍可存活。

品牌:BD Pharm Lyse™
监管状态:RUO
RRID:AB_2869057
应用:流式细胞仪(常规测试)

准备和存储标题文本

Store undiluted at 4°C.

1X diluted solutions may be stored at 4° C for up to 30 days.

推荐的实验流程

Preparation of 1X lysing solution:  Dilute the 10X concentrate 1:10 with distilled water. The pH of the 1X solution should fall within the range of pH 7.1-7.4.  Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.

Lysing procedure:

Note: The following procedure is only applied to human whole blood red blood cell lysis. Since applications vary, for other samples such as mouse spleen red blood cell lysis (lysing incubation time up to 3 minutes at 37°C), bone marrow red blood cell lysis, each investigator should optimize the condition to obtain optimal results.

1. Add 2.0 ml  of 1X lysing solution to each tube containing up to 200 µl of a whole blood plus monoclonal antibody mixture.

2. Gently vortex each tube immediately after adding the lysing solution.

3. Incubate at room temperature, protected from light, for 15 minutes.

4. Centrifuge 200 X g for 5 minutes.

5. Carefully aspirate supernatent, without disturbing pellet.

6. Add 2.0 ml 1X PBS containing 1% heat-inactivated fetal bovine serum and 0.1% sodium azide (PBS-FBS).

7. Centrifuge at 200 X g for 5 minutes.

8. Carefully aspirate supernatent, without disturbing pellet.

9. Resuspend pellet in 0.5 ml PBS-FBS or a fixative such as 2% formaldehyde for flow cytometric analysis.

Troubleshooting: Incomplete lysis may occur for several reasons: (1) The age of the specimen may affect red cell lysis, (2) The 1X lysing solution may not have been warmed to room temperature, or (3) After adding the lysing solution, the samples may not have been vortexed sufficiently. If incomplete lysis occurs (recognized by the presence of an excessive amount of visible red blood cells in the final, washed cell suspension), repeat lysing procedure. However, lysing more than two times is not recommended.

产品简介:

BD Pharm Lyse™裂解液是一种缓冲的,浓缩的(10倍)lv hua 铵基裂解试剂。当稀释到1X浓度并按推荐使用时,BD Pharm Lyse™在单克隆抗体染色后溶解红细胞。当用流式细胞术分析时,溶解液能很好的光散射分离淋巴细胞和红细胞碎片。BD Pharm Lyse™不含固定剂,所以白细胞在红细胞溶解后仍可存活。

产品应用:

流式细胞术(常规检测)

运输与储存:

保存在4°C未稀释。

1X稀释的溶液可在4°C下保存30天。

推荐的实验流程:

1X溶出液的制备:将10X浓缩液以1:10的比例用蒸馏水稀释。1X溶液的pH值应在pH 7.1-7.4范围内。必要时调整pH值。使用前将1X溶液加热至室温。100ml的10X浓缩液将产生足够溶解500个样品的1X溶液。

溶解过程:

注:以下步骤仅适用于人全血红细胞裂解。由于应用的不同,对于其他样品,如小鼠pi zang红细胞裂解(在37℃下裂解培养时间可达3分钟),gu sui红细胞裂解,每个研究人员应优化条件,以获得 结果。

  1. 每个试管中加入2.0 ml 1X溶解液,其中含有200μl全血和单克隆抗体混合物。
  2. 加入溶解液后,立即轻轻涡旋每个管。

3.室温避光孵育15分钟。

  1. 200xg离心5分钟。
  2. 小心吸出上清液,不要干扰颗粒。
  3. 加入2.0 ml 1X PBS。
  4. 200 X g离心5分钟。
  5. 小心吸出上清液,不要干扰颗粒。
  6. 用0.5 ml PBS-FBS或2%甲醛等固定剂重悬颗粒,用于流式细胞仪分析。

产品订购信息:

货号                名称                                         规格

555899    Lysing Buffer 10X Concentrate      100mL

 

BD 349202 FACS Lysing Solution

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures.

BD FACS™
Human
Buffered solution with <15% formaldehyde and <50% diethylene glycol.
Flow cytometry

BD FACS™ 裂解液用于在流式细胞术分析之前用单克隆抗体对人外周血细胞进行直接免疫荧光染色后裂解红细胞。

BD FACS 裂解液适用于试剂,例如 BD Tritest™ 或 BD Simultest™ 试剂和适当配备的流式细胞仪。 它可用于裂解/洗涤和裂解/免洗涤程序。

反应性:人类
存储缓冲区:含有 <15% 甲醛和 <50% 二甘醇的缓冲溶液。
应用:流式细胞仪

BD 349202  FACS Lysing Solution 溶血素 红细胞裂解液

DESCRIPTION

BD FACS™ lysing solution is intended for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

BD FACS lysing solution is appropriate for use with reagents such as BD Tritest™ or BD Simultest™ reagents and a suitably equipped flow cytometer. It may be used in both lyse/wash and lyse/no-wash procedures

PREPARATION AND STORAGE

BD FACS lysing solution (10X) is stable until the expiration date shown on the bottle label when stored as directed. Do not use this reagent if discoloration occurs or a precipitate forms.

FACS溶血素(BDIS Catalog No.349202)用于PBMCs,培养细胞与全血制备,主要有3个作用:
(1) 全血制备时用于溶解红细胞
(2) 固定外表位
(3) 辅助通透剂
     FACS溶血素使用前用无离子水1:10稀释,勿用PBS或其它缓冲液。而且FACS溶血素必须与FACS通透液一起使用,即使是培养细胞的检测。