分类目录归档：日本住友化学SUMICHIRAL

日本住友化学 SUMICHIRAL色谱柱 OA-SHELL P1

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日本住友化学 SUMICHIRAL色谱柱 OA-SHELL P1

日本住友化学代理
日本住友化学官网
欢迎访问日本住友化学SUMICHIRAL官网或者咨询我们获取更多相关产品信息。
Core-shell type chiral stationary phase
SUMICHIRALTM OA-SHELL P1
Advantages

SUMICHIRALTM OA-SHELL P1 is our first chiral stationary phase using optical active polymer. It is effective for chiral separation of compounds without amino group or carboxyl group，which are generally difficult to separate with conventional chiral stationary phases. Especially, it shows excellent resolution for aromatic chiral alcohol compounds. Core‐shell silica is used as a support and coated with poly (diphenylacetylene) derivative (optically active spiral polymer).

【Advantages of SUMICHIRALTM  OA-SHELL P1】

Chiral stationary phase using core-shell silica support

★ Analysis time could be shortened with conventional HPLC ★

  • Effective for chiral separation of compounds without amino group or carboxyl group ★
  • Easy method development with simple mobile phase compositions ★
  • Excellent durability, lot-to-lot repeatability ★

Unlike full porous particles, the core-shell particle has a non-porous core in the center and a thin porous layer outside of the core.

【Method development 】

SUMICHIRALTM OA-SHELL P1 makes it easy to set the conditions of mobile phase. The scheme of the general method development is shown in the right figure. For neutral compounds such as aromatic alcohols, it is not usually necessary to add special modifiers. For acidic compounds, add trifluoroacetic acid.

  • Do not use the following solvents as they will cause the column to deteriorate : tetrahydrofuran, 1,2-dichloroethane, chloroform, ethyl acetate, acetone, etc.

Standard mobile phase n-hexane/2-propanol（95/5）

Change the concentration of 2 -propanol and adjust the retention time

（concentration of 2-propanol 0～20％）

Change 2-propanol to ethanol

（concentration of ethanol 0～10％）

For acidic compounds etc., add trifluoroacetic acid

【Durability test】 （concentration of trifluoroacetic acid 0.02～0.1％）

SUMICHIRALTM OA-SHELL P1 has excellent durability despite the use of optically active polymers as chiral selectors. The mobile phase was continuously fed to the column, and the stability was evaluated under the following conditions. As a result, almost no change was observed in separation factor, resolution, retention time, and column pressure.

Conditions

mobile phase：n-hexane / ethanol（95/5）

flow rate：0.2 ｍL/min detection：UV254 nm

column temperature：25℃

sample：trans-2-phenyl-1-cyclohexanol 10

Ordering information

 

【Specification of SUMICHIRALTM OA-SHELL P1】

 

chiral selector core-shell silica support
particle size pore size specific surface area
poly (diphenylacetylene) 2.6 μm 250～280Å 40～60 m2/g
derivative

 

【List of SUMICHIRALTM OA-SHELL P1】
particle size internal diameter column length P/N
（μｍ） （mm） （mm）
50 C-SP01-3005W
2.6 3.0 100 C-SP01-3010W
150 C-SP01-3015W

【List of guard filter】

For column protection, we recommend porous glass guard filter “SUMIPAXTM Filter PG-ODS for semi-micro “.

product name contents P/N
SUMIPAXTM Filter Starter kit PG-ODS SUS holder 1 pcs GMODS001
for semi-micro filter 1 pcs
SUMIPAXTM Filter PG-ODS replacement filter 8 pcs GMODS002
Replacement filter for semi-micro
SUMIPAXTM  Holder for semi-micro SUS holder 1 pcs GMACC001
Connection kit for semi-micro ferrule screw 2 pcs GMACC002
SUS tube 1 pcs

【Column selection】

There are many kinds of SUMICHIRALTM series columns, so it is important to select the optimum column depending on the target compounds. Since you can use the database of chiral applications on our website and search application data from compound names and functional group information, please refer to the column selection.

  • SUMICHIRALTMand SUMIPAXTM are registered trademarks in Japan.
  • For prices and column sizes other than the above, please contact our distributor.

SUMICHIRAL OA系列色谱柱的选择与使用保养

发表于由

SUMICHIRAL OA系列色谱柱的选择与使用保养

SUMICHIRAL 选择选择方法

  • SUMICHIRAL OA-2000 series
  • SUMICHIRAL OA-3000 series
  • SUMICHIRAL OA-4000 series
  • SUMICHIRAL OA-5000 series
  • SUMICHIRAL OA-6000 series
  • SUMICHIRAL OA-7000 series
  • SUMICHIRAL OA-8000 series
  • SUMICHIRAL AGP , CBH , HSA
  • SUMICHIRAL Columns Product list

SUMICHIRAL OA series is a pioneer of chiral HPLC stationary phase using low molecular weight chiral selector.

SUMICHIRAL OA 系列手性柱是高性能的分离光学异构体的HPLC色谱柱。SUMICHIRAL OA色谱柱能直接分离各种异构体，并可从非对应异构体中分离异构体，这些非对应异构体中可能存在氢键，电荷转移及主客体等相互作用。OA系列手性柱能精确测定各种手性化合物的纯度，如药物、农药、香料中手性结构的组成。
OA-2000系列柱，改进刷型（Amide），不对称碳原子键合CONH基团
OA-2000系列柱可直接分离芳香类、酯类、羧酸类和醇类化合物的异构体。如OA-2000色谱柱分离合成除虫菊酯，OA-2500可分离布洛芬药物。
OA-3000系列柱，改性刷型（尿素），不对称碳原子键合NHCONH基团
OA-3000系列色谱柱可有效分离羧酸类化合物，尤其是乙酰基和聚氨酯的氨基酸化合物。OA-3300色谱柱能直接分离布洛芬药物，乙酰胺基酸，BOC-氨基酸和苄基氨基酸。
OA-4000系列柱，改进刷型，胺和氨基酸两个手性中心键合NHCONH基团
OA-4000系列柱通过电荷转移和氢键相互作用达到手性识别，能分离各种各样的化合物，如胺类和氨基醇的药物，醇类，酯类和酰胺类化合物可用正相模式分离。尤其是酰胺和聚氨酯的衍生物，醇类等柱效高。
OA-5000和OA-6000系列柱在反相模式中通过配位交换相互作用进行手性识别。OA-5000手性配位体是青霉胺涂敷在ODS硅胶表面，OA-6000手性配位体是酒石酸衍生物涂敷在ODS硅胶表面，限制了添加到流动相的有机溶剂用量，且OA-5000和OA-6000系列柱的流动相中必须包含Cu2+离子。该系列柱不但能有效的分离氨基酸，羟基酸的对映异构体，而且能分离铜螯合形成的化合物，如氨基醇，胺，羧酸，氨基内酰胺和二肽。尤其是OA-5000柱应用极为广泛，OA-6000能有效分离β-氨基酸，β-羟基酸和亲水性阿明酸。
OA-7000柱手性固定相是β-环糊精通过新型的空间模式键合硅胶。可在反相条件下分离大量的消旋体，包括酮，胺，氨基酸衍生物。
OA-8000柱手性固定相是冠醚手性键合氨丙基硅胶，可有效的分离胺，氨基醇和氨基酸的对映异构体，尤其是疏水性胺。

Features

  • Enables direct high quality separation of optical isomers.

    Enables direct high quality separation of optical isomers by a large number of theoretical plate.

  • Elution order inversion

    In the SUMICHIRAL OA series, low molecular weight optically active compounds are immobilized onto the stationary phase. By immobilizing an antipode of this optically active component, the elution order of the target substance can be reversed. (Excluding OA-4800/4900/7000 series, 8000, AGP, CBH, and HSA)

  • Applicable for LC-MS

    SUMICHIRAL OA-2500/3000 series is applicable for LC-MS as the recommended mobile phase is methanol containing ammonium acetate.

  • Supercritical Fluid Chromatography (SFC)

    SUMICHIRAL OA-2000 and OA-4000 series are also available for Supercritical fluid chromatography (SFC).

Product lineup

Separation of enantiomers takes place due to the minor energy difference between the solute and the stationary phase caused by diastereomeric interactions (e.g., hydrogen bonding, charge transfer, coordinate bonding).
SUMICHIRAL column types, optically active component, separation mode and target substances are summarized in the table below.

Optical isomers separation columns (low molecular weight isomers)

Amide-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-2000 (R)-Phenylglycine Normal phese Esters, alcohols, amides, ketones OA-2000S
OA-2000I Esters, ketones OA-2000SI
OA-2500 (R)-1-Naphtyl glycine Reversed phese* Carboxylic acids, alcohols, esters, ketones OA-2500S
OA-2500I Normal phese Esters, alcohols, ketones OA-2500SI
Reversed phese* : non-aqueous reversed phase (ammonium acetate/methanol solution)
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-2000 series.

Urea-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-3100 (S)-Valine Reversed phase* Carboxylic acids
(N-amino acid derivatives)		 OA-3100R
OA-3200 (S)-tert-Leucine OA-3200R
OA-3300 (R)-Phenylglycine OA-3300S
OA-4000 (S)-Valine, (S)-NEA Normal phese Amines, amides, aminoalcohols, alcohols, esters, phosphamides OA-4000R
OA-4100 (S)-Valine, (R)-NEA OA-4100R
OA-4400 (S)-Proline, (S)-NEA OA-4400R
OA-4500 (S)-Proline, (R)-NEA OA-4500R
OA-4600 (S)-tert-Leucine,
(S)-NEA		 OA-4600R
OA-4700 (S)-tert-Leucine,
(R)-NEA		 OA-4700R
OA-4800 (S)-Indoline-2-carboxylic acid,
(S)-NEA		 　—
OA-4900 (S)-Indoline-3-carboxylic acid,
(R)-NEA		 　—
Reversed phese* : non-aqueous reversed phase (ammonium acetate/methanol solution)
(S)-NEA ： (S)-1- (α-Naphtyl) ethylamine
(R)-NEA ： (R)-1- (α-Naphtyl) ethylamine
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-3000 series.
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-4000 series.

Ligand exchange-type

SUMICHIRAL Opticall acitive components Separation mode Target substances Columns for elution order inversion SUMICHIRAL OA
OA-5000 (D)-Penicillamine Reversed phase Amino acids, hydroxy acids, amino alcohols, amines OA-5000L
OA-6000 (L)-Tartaric acid,
(R)-NEA		 OA-6000R
OA-6100 (L)-Tartaric acid,
(S)-Valine, (S)-NEA		 OA-6100R
(S)-NEA ： (S)-1- (α-Naphtyl) ethylamine
(R)-NEA ： (R)-1- (α-Naphtyl) ethylamine
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-5000 series.
  • Click here for product details, separation examples, and product list of the SUMICHIRAL OA-6000 series.

Host-guest-type

SUMICHIRAL Opticall acitive components Separation mode Target substances
OA-7000 β-Cyclodexitrin Reversed phase Flavonoid derivatives, acids, neutral compunds
OA-7100 β-Cyclodexitrin Acids, neutral compounds
OA-7500 Methyl-β-cyclodexitrin Alcohols, esters
OA-7600 Methyl-α-cyclodexitrin Neutral compounds
OA-7700 Acetyl-β-cyclodexitrin Amines
OA-8000 Chiral pseudo-crown ether Normal phase Primary amines
Reversed phase

 

  • Click here for product details, separation examples, and price table of the SUMICHIRAL OA-7000 series.
  • Click here for product details, separation examples, and price table of the SUMICHIRAL OA-8000 series.

Optical isomer separation columns (proteins)

SUMICHIRAL Opticall acitive components Separation mode Target substances
AGP α1-acid glycoprotein Reversed phase Acids, bases, neutral compounds, amides, ketones
CBH Cellobiohydrolase Reversed phase Bases
HSA Human serum albumin Reversed phase Acids

 

  • Click here for product details, separation examples, and prioduct list of the SUMICHIRAL AGP, CBH and HSA.

日本住友化学 SUMICHIRAL OA-4000 series

发表于由

日本住友化学 SUMICHIRAL OA-4000 series

日本住友化学代理

日本住友化学官网

欢迎访问日本住友化学SUMICHIRAL官网或者咨询我们获取更多相关产品信息。

SUMICHIRAL 选择选择方法

  • SUMICHIRAL OA-2000 series
  • SUMICHIRAL OA-3000 series
  • SUMICHIRAL OA-4000 series
  • SUMICHIRAL OA-5000 series
  • SUMICHIRAL OA-6000 series
  • SUMICHIRAL OA-7000 series
  • SUMICHIRAL OA-8000 series
  • SUMICHIRAL AGP , CBH , HSA
  • SUMICHIRAL Columns Product list

製品名

  • SUMICHIRAL OA-4000、OA-4100
  • SUMICHIRAL OA-4400、OA-4500
  • SUMICHIRAL OA-4600、OA-4700
  • SUMICHIRAL OA-4800、OA-4900

SUMICHIRAL OA-4000、OA-4100

Features

Used for normal phase systems
Applicable for a wide variety of compounds,such as aromatic amines and amides
Able to inverse the elution order
Supports SFC (supercritical fluid chromatography)

Active site

Product specification

Product name SUMICHIRAL OA-4000 SUMICHIRAL OA-4100
Chiral selectors (S)-Valine，
(S)-1-(α-Naphtyl) ethylamine		 (S)-Valine，
(R)-1-(α-Naphtyl) ethylamine
Support High purity silica gel
Particle size 5μm
Supporting mode Chemically bonded
Recommended mobile phase Hexane/ethanol/trifluoroacetic acid
Target compounds Amines, amides, aminoalcohols, alcohols, esters, phosphamides
Columns for elution order inversion SUMICHIRAL OA-4000R SUMICHIRAL OA-4100R

Product list

Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4000 4 10 C-4001-4001W
150 C-4001-4015W
250 C-4001-4025W
4.6 50 C-4001-4605W
150 C-4001-4615W
250 C-4001-4625W
8 250 C-4001-8025W
10 250 C-400110025W
20 250 C-400120025W
SUMICHIRAL OA-4100 4 10 C-4101-4001W
150 C-4101-4015W
250 C-4101-4025W
4.6 50 C-4101-4605W
150 C-4101-4615W
250 C-4101-4625W
8 250 C-4101-8025W
10 250 C-410110025W
20 250 C-410120025W
Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4000R 4 10 C-4011-4001W
150 C-4011-4015W
250 C-4011-4025W
4.6 50 C-4011-4605W
150 C-4011-4615W
250 C-4011-4625W
8 250 C-4011-8025W
10 250 C-401110025W
20 250 C-401120025W
SUMICHIRAL OA-4100R 4 10 C-4111-4001W
150 C-4111-4015W
250 C-4111-4025W
4.6 50 C-4111-4605W
150 C-4111-4615W
250 C-4111-4625W
8 250 C-4111-8025W
10 250 C-411110025W
20 250 C-411120025W
  • ※Note: Those shown in red are standard size for analysis.
    Internal diameter of 4 mm and length of 10 mm is a guard column.
    SUMIPAX Filter can be used in place of a guard column.
  • Click here for SUMIPAX Filters

Applications

SUMICHIRAL OA-4000(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol (100 : 0.05)
UV 230 nm ｜ 1.0 mL / min ｜ 1μL (1 mg / mL)

SUMICHIRAL OA-4000(5μm, 4.6mm i.d.×250mm)
hexane / ethanol (93 : 7)
UV 254 nm ｜ 1.0 mL / min

SUMICHIRAL OA-4100(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol / methanol / trifluoroacetic acid (70 : 30 : 0.5 : 0.5)
UV 254 nm ｜ 1.0 mL / min ｜ 5μL (5 mg / mL)

SUMICHIRAL OA-4100(5μm, 4.6mm i.d.×250mm)
hexane / ethanol (98 : 2)
UV 230 nm ｜ 1.0 mL / min ｜ 1μL (1 mg / mL)

  • SUMICHIRAL Database
  • Guidelines for Selection of SUMICHIRAL Columns

SUMICHIRAL OA-4400、OA-4500

Features

  • Used for normal phase systems
  • Applicable for a wide variety of compounds, such as aromatic amines and amides
  • Able to inverse the elution order
  • Supports SFC (supercritical fluid chromatography)

Active site

Product specification

Product name SUMICHIRAL OA-4400 SUMICHIRAL OA-4500
Chiral selectors (S)-Proline，
(S)-1-(α-Naphtyl) ethylamin		 (S)-Proline，
(R)-1-(α-Naphtyl) ethylamin
Support High purity silica gel
Particle size 5μm
Supporting mode Chemically bonded
Recommended mobile phase Hexane/ethanol/trifluoroacetic acid
Target compounds Amines, amides, aminoalcohols, esters, phosphamides
Columns for elution order inversion SUMICHIRAL OA-4400R SUMICHIRAL OA-4500R

Product list

Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4400 4 10 C-4401-4001W
150 C-4401-4015W
250 C-4401-4025W
4.6 50 C-4401-4605W
150 C-4401-4615W
250 C-4401-4625W
8 250 C-4401-8025W
10 250 C-440110025W
20 250 C-440120025W
SUMICHIRAL OA-4500 4 10 C-4501-4001W
150 C-4501-4015W
250 C-4501-4025W
4.6 50 C-4501-4605W
150 C-4501-4615W
250 C-4501-4625W
8 250 C-4501-8025W
10 250 C-450110025W
20 250 C-450120025W
Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4400R 4 10 C-4411-4001W
150 C-4411-4015W
250 C-4411-4025W
4.6 50 C-4411-4605W
150 C-4411-4615W
250 C-4411-4625W
8 250 C-4411-8025W
10 250 C-441110025W
20 250 C-441120025W
SUMICHIRAL OA-4500R 4 10 C-4511-4001W
150 C-4511-4015W
250 C-4511-4025W
4.6 50 C-4511-4605W
150 C-4511-4615W
250 C-4511-4625W
8 250 C-4511-8025W
10 250 C-451110025W
20 250 C-451120025W
  • ※Note: Those shown in red are standard size for analysis.
    Internal diameter of 4 mm and length of 10 mm is a guard column.
    SUMIPAX Filter can be used in place of a guard column.
  • Click here for SUMIPAX Filters

Applications

SUMICHIRAL OA-4400(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol / methanol / trifluoroacetic acid (98 : 1 : 1 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 5μL (20 mg / mL)

SUMICHIRAL OA-4400(5μm, 4.6mm i.d.×250mm)
hexane / 1,2-dichloroethane / ethanol / acetic acid(500 : 150 : 10 : 1)
UV 254 nm ｜ 1.0 mL / min

SUMICHIRAL OA-4500(5μm, 4.0mm i.d.×250mm)
hexane / tetrahydrofuran / methanol / trifluoroacetic acid (60 : 35 : 5 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 1μL (1 mg / mL)

SUMICHIRAL OA-4500(5μm, 4.0mm i.d.×250mm)
hexane / tetrahydrofuran / methanol / trifluoroacetic acid (60 : 35 : 5 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 1μL (1 mg / mL)

  • SUMICHIRAL Database
  • Guidelines for Selection of SUMICHIRAL Columns

SUMICHIRAL OA-4600、OA-4700

Features

  • Used for normal phase systems
  • Applicable for a wide variety of compounds, such as aromatic amines and amides
  • Able to inverse the elution order
  • Supports SFC (supercritical fluid chromatography)

Active site

Product specification

Product name SUMICHIRAL OA-4600 SUMICHIRAL OA-4700
Chiral selectors (S)-tert-Leucine，
(S)-1-(α-Naphtyl) ethylamine		 (S)-tert-Leucine，
(R)-1-(α-Naphtyl) etylamine
Support High purity silica gel
Partilcle size 5μm
Supporting mode Chemically bonded
Recommended mobile phase Hexane/ethanol/trifluoroacetic acid
Target compounds Amines, amides, aminoalcohols, alcohols, esters, phosphamides
Columns for elution order inversion SUMICHIRAL OA-4600R SUMICHIRAL OA-4700R

Product list

Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4600 4 10 C-4601-4001W
150 C-4601-4015W
250 C-4601-4025W
4.6 50 C-4601-4605W
150 C-4601-4615W
250 C-4601-4625W
8 250 C-4601-8025W
10 250 C-460110025W
20 250 C-460120025W
SUMICHIRAL OA-4700 4 10 C-4701-4001W
150 C-4701-4015W
250 C-4701-4025W
4.6 50 C-4701-4605W
150 C-4701-4615W
250 C-4701-4625W
8 250 C-4701-8025W
10 250 C-470110025W
20 250 C-470120025W
Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4600R 4 10 C-4611-4001W
150 C-4611-4015W
250 C-4611-4025W
4.6 50 C-4611-4605W
150 C-4611-4615W
250 C-4611-4625W
8 250 C-4611-8025W
10 250 C-461110025W
20 250 C-461120025W
SUMICHIRAL OA-4700R 4 10 C-4711-4001W
150 C-4711-4015W
250 C-4711-4025W
4.6 50 C-4711-4605W
150 C-4711-4615W
250 C-4711-4625W
8 250 C-4711-8025W
10 250 C-471110025W
20 250 C-471120025W
  • ※Note: Those shown in red are standard size for analysis.
    Internal diameter of 4 mm and length of 10 mm is a guard column.
    SUMIPAX Filter can be used in place of a guard column.
  • Click here for SUMIPAX Filters

Applications

SUMICHIRAL OA-4600(5μm, 4.6mm i.d.×250mm)
hexane / ethanol (98 : 2)
UV 254 nm ｜ 1.0 mL / min

SUMICHIRAL OA-4600(5μm, 4.0mm i.d.×250mm)
hexane / 1,2-dichloroethane / methanol / trifluoroacetic acid (245 : 140 : 15 : 1)
UV 254 nm ｜ 1.0 mL / min ｜ 1μL (10 mg / mL)

SUMICHIRAL OA-4700(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol / methanol / trifluoroacetic acid (85 : 10 : 5 : 0.2)
UV 220 nm ｜ 1.0 mL / min ｜ 0.5μL (5 mg / mL)

SUMICHIRAL OA-4700(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol / methanol / trifluoroacetic acid (85 : 10 : 5 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 10μL (1 mg / mL)

  • SUMICHIRAL Database
  • Guidelines for Selection of SUMICHIRAL Columns

SUMICHIRAL OA-4800、OA-4900

Features

  • Used for normal phase systems
  • Applicable for a wide variety of compounds, such as aromatic amines and amides
  • Supports SFC (supercritical fluid chromatography)

Active site

Product specification

Product name SUMICHIRAL OA-4800 SUMICHIRAL OA-4900
Chiral selectors (S)-Indoline-2-carboxylic acid，
(S)-1-(α-Naphtyl) ethylamine		 (S)-Indoline-2-carboxylic acid，
(R)-1-(α-Naphtyl) ethylamine
Support High purity silica gel
Particle size 5μm
Supporting mode Chemically bonded
Recommended mobile phase Hexane/ethanol/trifluoroacetic acid
Target compounds Amines, amides, aminoalcohols, alcohols, esters, phosphamides

Product list

Product name Internal diameter (mm) Length (mm) Product code
SUMICHIRAL OA-4800 4 10 C-4801-4001W
150 C-4801-4015W
250 C-4801-4025W
4.6 50 C-4801-4605W
150 C-4801-4615W
250 C-4801-4625W
8 250 C-4801-8025W
10 250 C-480110025W
20 250 C-480120025W
SUMICHIRAL OA-4900 4 10 C-4901-4001W
150 C-4901-4015W
250 C-4901-4025W
4.6 50 C-4901-4605W
150 C-4901-4615W
250 C-4901-4625W
8 250 C-4901-8025W
10 250 C-490110025W
20 250 C-490120025W
  • ※Note: Those shown in red are standard size for analysis.
    Internal diameter of 4 mm and length of 10 mm is a guard column.
    SUMIPAX Filter can be used in place of a guard column.
  • Click here for SUMIPAX Filters

Applications

SUMICHIRAL OA-4800(5μm, 4.6mm i.d.×250mm)
hexane / 2-propanol / methanol / trifluoroacetic acid (70 : 20 : 10 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 0.5μL (2 mg / mL)

SUMICHIRAL OA-4800(5μm, 4.6mm i.d.×250mm)
hexane / 1,2-dichloroethane / methanol / trifluoroacetic acid (70 : 20 : 10 : 0.2)
UV 254 nm ｜ 1.0 mL / min ｜ 5μL (3 mg / mL)

SUMICHIRAL OA-4900(5μm, 4.6mm i.d.×250mm)
hexane / ethanol (100 : 1)
UV 254 nm ｜ 1.0 mL / min

SUMICHIRAL OA-4900(5μm, 4.6mm i.d.×250mm)
hexane / 1,2-dichloroethane methanol / trifluoroacdetic acid (240 : 140 : 20 : 1)
UV 280 nm ｜ 1.0 mL / min

  • SUMICHIRAL Database
  • Guidelines for Selection of SUMICHIRAL Columns

日本住友化学 SUMICHIRAL OA-3000 series系列色谱柱

发表于由

日本住友化学 SUMICHIRAL OA-3000 series系列色谱柱

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日本住友化学官网

欢迎访问日本住友化学SUMICHIRAL官网或者咨询我们获取更多相关产品信息。

SUMICHIRAL 选择选择方法

特征

Usable for both normal phase systems and reversed-phase systems (polar ionic phase) (water is not usable)
Applicable for LC-MS ⇒ Ammonium acetate/methanol solution
Able to reverse the elution order

可用于正常相系统和反相系统(极性离子相)(水不可用)
适用于LC-MS⇒醋酸铵/甲醇溶液
能够逆转洗脱顺序

活动位点

Product name SUMICHIRAL OA-3100 SUMICHIRAL OA-3200 SUMICHIRAL OA-3300
Chiral selectors (S)-Valine (S)-tert-Leucine (R)-Phenylglycine
Support High purity silica gel
Particle size 5μm
Supporting mode Chemically bonded
Recommended mobile phase 【Nomal phase】 Hexane/ethanol/trifluoroacetic acid
【Reversed phase (polar ionic phase)】 Methanol containing ammonium acetate
Target compounds 【Normal phase】 Alcohols
【Reversed phase (polar ionic phase)】 Carboxylic acids (amino N derivatives)
Columns for elution order inversion SUMICHIRAL OA-3100R SUMICHIRAL OA-3200R SUMICHIRAL OA-3300S

 

产品规格

产品名称 骨关节炎-3100 骨关节炎-3200 骨关节炎-3300
手性选择器 (S)-Valine (S)-叔亮氨酸 (R)-苯甘氨酸
支撑 高纯度硅胶
颗粒尺寸 5μm
支撑模式 化学键合
推荐流动阶段 [正常相]正己烷/乙醇/三氟乙酸
[反相(极性离子相)]含乙酸铵甲醇
目标化合物 [正常相]酒精
[反相(极性离子相)]羧酸(氨基N衍生物)
洗脱反相柱 亚型OA-3100 R 离体OA-3200 R 亚临界OA-3300 S

产品清单

产品名称 内径(毫米) 长度(毫米) 产品代码
骨关节炎-3100 2 150 C-3101-2015W
250 C-3101-2025 W
4 10 C-3101-4001W
150 C-3101-4015W
250 C-3101-4025 W
4.6 50 C-3101-4605W
150 C-3101-4615 W
250 C-3101-4625 W
8 250 C-3101-8025W
10 250 C-310110025W
20 250 C-310120025W
骨关节炎-3200 2 150 C-3201-2015W
250 C-3201-2025 W
4 10 C-3201-4001W
150 C-3201-4015W
250 C-3201-4025 W
4.6 50 C-3201-4605W
150 C-3201-4615 W
250 C-3201-4625 W
8 250 C-3201-8025W
10 250 C-320110025W
20 250 C-320120025W
骨关节炎-3300 2 150 C-3301-2015W
250 C-3301-2025 W
4 10 C-3301-4001W
150 C-3301-4015 W
250 C-3301-4025 W
4.6 50 C-3301-4605 W
150 C-3301-4615 W
250 C-3301-4625 W
8 250 C-3301-8025W
10 250 C-330110025W
20 250 C-330120025W
产品名称 内径(毫米) 长度(毫米) 产品代码
亚型OA-3100 R 2 150 C-3111-2015W
250 C-3111-2025 W
4 10 C-3111-4001W
150 C-3111-4015 W
250 C-3111-4025 W
4.6 50 C-3111-4605W
150 C-3111-4615 W
250 C-3111-4625 W
8 250 C-3111-8025W
10 250 C-311110025W
20 250 C-311120025W
离体OA-3200 R 2 150 C-3211-2015 W
250 C-3211-2025 W
4 10 C-3211-4001W
150 C-3211-4015 W
250 C-3211-4025 W
4.6 50 C-3211-4605 W
150 C-3211-4615 W
250 C-3211-4625 W
8 250 C-3211-8025 W
10 250 C-321110025W
20 250 C-321120025W
亚临界OA-3300 S 2 150 C-3311-2015 W
250 C-3311-2025 W
4 10 C-3311-4001W
150 C-3311-4015 W
250 C-3311-4025 W
4.6 50 C-3311-4605 W
150 C-3311-4615 W
250 C-3311-4625 W
8 250 C-3311-8025 W
10 250 C-331110025W
20 250 C-331120025W
  • ※注：用红色表示的是分析的标准尺寸。
    内径为4mm，长度为10 mm为防护柱。
    SUMIPAX过滤器可以用来代替保护列。
  • 单击此处获取SUMIPAX过滤器

应用

亚临界OA-3100(5μm，4.0mm I.D.×250 mm)
0.03mol/L醋酸铵在甲醇中的含量
UV 254 nm/1.0 mL/min

-3100(5μm，4.6mm I.D.×250毫米)
0.01 mol/L醋酸铵在甲醇中的含量
UV 254 nm=1.0 mL/min 10μL(7 mg/mL)

亚显微OA-3200(5μm，4.6mm I.D.×250 mm)
正己烷/2-丙醇/甲醇(70：20：10)
UV 254 nm/1.0 mL/min

亚显微OA-3200(5μm，4.6mm I.D.×250 mm)
甲醇中0.005 mol/L醋酸铵
UV 254 nm/0.8mL/min

-3300(5μm，4.6mm I.D.×250毫米)
0.01 mol/L醋酸铵在甲醇中的含量
紫外254 nm-1.0 mL/min-0.4μL(10 mg/mL)

-3300(5μm，4.6mm I.D.×250毫米)
0.01 mol/L醋酸铵在甲醇中的含量
UV 254 nm=1.0mL/min 2μL(4mg/mL)

日本住友化学 SUMICHIRAL色谱柱

发表于由

日本住友化学 SUMICHIRAL色谱柱

日本住友化学代理

日本住友化学官网

欢迎访问日本住友化学SUMICHIRAL官网或者咨询我们获取更多相关产品信息。

SUMICHIRAL OA columns are high-performance chiral columns for
enantiomer separation by HPLC. On SUMICHIRAL OA columns direct
separation of various enantiomers can be realized effectively.
Enantiomeric separation is achieved from the various diastereomeric
interactions such as hydrogen bonding, charge transfer and host-guest
interactions, etc. SUMICHIRAL OA columns are very useful for the
accurate determination of the optical purity and for the preparation of
pure enantiomers of biologically active compounds such as
pharmaceuticals, pesticides, and perfumes.
■ Improved Pirkle Type
■ Ligand exchange Type
■ Host-guest Type

Improved Pirkle Type 1 Improved Pirkle Type 1

■Amide Type : Asymmetric carbon atoms are bonded directly with CONH group
OA-2000 series have a 3,5-dinitrobenzoyl group as theπ-acid and may interact with the solute
molecule by charge transfer, hydrogen bonding, etc. The enantiomers of aromatic compounds, esters,
carboxylic acids and alcohols may be directly separated on OA-2000 series. OA-2000 is especially
effective for pyrethroidal esters, OA-2500 for carboxylic acids such as profen-drugs.
■Urea Type : Asymmetric carbon atoms are bonded directly with NHCONH group
OA-3000 series have 3,5-dinitrophenylurea group as theπ-acid and, in the reverse phase mode,
promote chiral discrimination by charge transfer, hydrogen bonding, etc. In general OA-3000 series are
effective for carboxylic acids, and especially for acetyl- and urethane-amino acids, dansylamino acids.
OA-3300 offers good direct separation for profen-drugs, acetyl-amino acids, BOC-amino acids and
benzyl-amino acids.

Main columns : SUMICHIRAL OA-2500, SUMICHIRAL OA-3300

Special Merits of SUMICHIRAL OA

The large number of theoretical plates of the columns offer high
resolution.
The packing materials have chemical stability and the columns have
long life.
The enantiomeric stationary phases give the inverse elution orders
and so accurate determination of the optical purity and efficient
preparation of the enantiomer are attained.

Improved Pirkle Type 2 Improved Pirkle Type 2

Two chiral centers at amine and amino acid are bonded with NHCONH group
OA-4000 series have a naphthyl group as theπ-base, and two chiral centers at amine and amino
acid group. By charge transfer, hydrogen bonding, etc., chiral discrimination is acheived, and a wide
variety of compounds such as pharmaceuticals of amine and amino alcohols, alcohols, esters and
amides can be directly resolved in the normal phase mode. Amide and urethane derivatives of amines,
alcohols, etc. can be resolved effectively

Ligand exchange Type Ligand exchange Type

■The chiral components are coated hydrophobically on ODS
OA-5000 and 6000 series offer chiral discrimination by ligand exchange interaction in the
reversed phase mode. The chiral ligands such as penicillamine (OA-5000) or tartaric acid
derivatives (OA-6000 series) are coated on ODS silica, though the volume of organic solvents
added to the mobile phase is limited. Mobile phases including Cu++ ions are used in these
columns. They are effective for direct enantiomer separation of not only amino acids, hydroxy acids
but also copper-chelate forming compounds such as amino alcohols, diamines, dicarboxylic acids,
aminolactames and dipeptides. Especially OA-5000 can be applied for extremely wide range, while
OA-6100 is effective for β-amino acids,β-hydroxy acids and hydrophilic amino acids.

Main columns : SUMICHIRAL OA-5000, SUMICHIRAL OA-6100

Host-Guest Type

■Cyclodextrin bonded chiral stationary phase with novel spacer
OA-7000 is a novel chiral stationary phase with β-cyclodextrin bonded to the silica gel via new type of
spacer. A large number of racemates, including ketones, amines, and amino acid derivatives can be
separated under reversed phase conditions.
(1) Sharp peaks and high theoretical plate numbers are obtained.
Improved peak shape is due to the effect of hydrophilic spacer moiety which prevents secondary
interactions between the silica gel and the sample molecules.
(2) Popular reversed phase conditions can be used.
■Novel Chiral Stationary phase bonded with crown ether
OA-8000 is a novel chiral stationary phase bonded with chiral crown ether to aminopropyl silica gel. This
is very effective for enantiomer separations of amines, aminoalcohols and amino acids, especially for
hydrophobic amines.
(1) Stationary phase is covalent bond type and very stable.
(2) Both reversed and normal phases can be used.
(3) Sharp peaks and high theoretical plate numbers are obtained

Standard type Inverted type Mode**  
SUMICHIRAL Chiral component SUMICHIRAL  
   
OA-2000 (R)-phenylglycine OA-2000S NP  
☆ OA-2500 (R)-1-naphthylglycine OA-2500S RP  
OA-3100 (S)-valine OA-3100R NP,RP  
OA-3200 (S)-tert-leucine OA-3200R NP,RP  
☆ OA-3300 (R)-phenylglycine OA-3300S NP,RP  
OA-4000 (S)-valine (S)-1-(α-naphthyl)ethylamine OA-4000R NP  
OA-4100 (S)-valine (R)-1-(α-naphthyl)ethylamine OA-4100R NP  
OA-4400 (S)-proline (S)-1-(α-naphthyl)ethylamine OA-4400R NP  
OA-4500 (S)-proline (R)-1-(α-naphthyl)ethylamine OA-4500R NP  
OA-4600 (S)-tert-leucine (S)-1-(α-naphthyl)ethylamine OA-4600R NP  
☆ OA-4700 (S)-tert-leucine (R)-1-(α-naphthyl)ethylamine OA-4700R NP  
OA-4800 (S)-indoline-2-carboxylic acid ＊ NP  
(S)-1-(α-naphthyl)ethylamine  
       
☆ OA-4900 (S)-indoline-2-carboxylic acid ＊ NP  
(R)-1-(α-naphthyl)ethylamine  
       
☆ OA-5000 (D)-penicillamine OA-5000L RP  
OA-6000 (L)-tartaric acid  (S)-1-(α-naphthyl)ethylamine OA-6000R RP  
OA-6100 (L)-tartaric acid, (S)-valine OA-6100R RP  
(R)-1-(α-naphthyl)ethylamine  
   
       
☆ OA-7000 β-cyclodextrin with novel spacer ＊ RP  
☆ OA-8000 chiral pseudo 18-crown-6 ether ＊ NP,RP

Notation on the SUMICHIRAL database

SUMICHIAL标签的表示法