Kapa biosystems品牌介绍

Kapa biosystems品牌介绍产品列表

上海金畔生物代理销售Kapa biosystems品牌产品,欢迎新老客户访问Kapa biosystems官网或者咨询我们获取更多相关信息。
Kapa biosystems

Kapa biosystems成立于2006年,其生产基地位于南非开普敦。致力于研发、生产下一代PCR(Next-Generation PCR)所需的生物酶。分子进化技术(酶工程)打破了现有的研究局限,为生物研究开创了一片新天地。公司技术团队在蛋白质工程、基因组学、蛋白质组学、合成生物学方面获得非常突出的成绩。Kapa biosystems公司追求科学研究与市场化运作的完美结合,执着于“科技创造完美产品,助力生命科学研究”这一最高宗旨。

产品:

应用:产品已被广泛应用于DNA扩增、二代测序、分子诊断等领域。

Kapa biosystems品牌产品线列表

Applications
Colony PCR
High-fidelity PCR
Fast PCR
Sanger Sequencing
Single-protocol PCR
Genotyping
Cloning
Crude Sample PCR
GC-rich PCR
Mutagenesis
Long-range PCR
Direct Blood PCR
Direct Plant PCR
Multiplex PCR
Gene Expression
Mouse Genotyping
Food and Water Safety Testing
Whole Genome
RNA Sequencing
Targeted Sequencing
FFPE Sample Sequencing
ChIP Sequencing
Methyl Sequencing
Epigenetics
Automated Solutions
NEXT GENERATION SEQUENCING
qPCR
PCR
Products
KAPA HiFi
KAPA HiFi Uracil+APA2G Robust
KAPA2G Fast Multiplex
KAPA3G Plant
KAPA2G Fast
KAPA Mouse Genotyping
KAPA Long Range
KAPA Blood
KAPA Taq
KAPA Express Extract
KAPA DNA Ladders
KAPA dNTPs
KAPA T4 DNA Ligase
KAPA Rapid Ligation System
KAPA SYBR FAST
KAPA SYBR FAST One-StepKAPA PROBE FORCE
KAPA PROBE FAST
KAPA HRM FAST
Sample QC
DNA Library Preparation
ibrary Amplification
RNA Library Preparation
Library Quantification
Beads
NEXT GENERATION SEQUENCING
qPCR
PCR

热销产品:

品牌 货号 产品名称 规格
KAPA KK2502 HiFiHot Start 250 units
KAPA KK2602 KAPA 热启动HiFi

高保真酶 ReadyMix

6.25ml
KAPA KK2801 KAPAHiFiHotStart Uracil+ ReadyMix (1 x 1.25 mL) 50 x 50 μL reactions
KAPA KK5516 KAPA 2G Robust HotStart PCR Kit with dNTPs 250 U
KAPA KK5802 KAPA 2G Fast MultiplexPCR Kitz9试剂 500 x 25 μl rxns
KAPA KK8002 KAPA 纯化磁珠 (60ml) 60ml
KAPA KK8504 KAPA Hyper Prep (96rxn) 96rxn
KAPA Kk8514 KAPA DNA  HyperPlus文库构建试剂盒 (96个反应) 96rxn

KAPA 2G Fast DNA聚合酶及Ready Mix

KAPA 2G Fast DNA聚合酶及Ready Mix
KAPA 2G FAST DNA聚合酶是新一代聚合酶,较野生型Taq DNA聚合酶和其他DNA聚合酶更快速合成DNA,显著缩短了PCR时间且不改变反应表现。对于小于1kb的人基因组DNA片段,低至1个拷贝,1秒的延伸时间即可。
产品优势:
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货号 简介 备注
KK5008 KAPA 2G Fast PCR Kit with dNTPs 100 U
KK5009 KAPA 2G Fast PCR Kit with dNTPs 250 U
KK5020 KAPA 2G Fast PCR Kit 100 U
KK5021 KAPA 2G Fast PCR Kit 250 U
KK5101 KAPA 2G Fast ReadyMix PCR Kit + dye 100 x 25 µl rxns
KK5102 KAPA 2G Fast ReadyMix PCR Kit + dye 500 x 25 µl rxns
KK5500 KAPA 2G Fast HotStart PCR Kit with dNTPs 500 U
KK5501 KAPA 2G Fast HotStart PCR Kit 500 U
KK5502 KAPA 2G Fast HotStart PCR Kit with dNTPs 250 U
KK5503 KAPA 2G Fast HotStart PCR Kit 250 U
KK5510 KAPA 2G Fast HotStart PCR Kit with Mg-free buffer M,with dNTPs 500 U
KK5511 KAPA 2G Fast HotStart PCR Kit with Mg-free buffer M 500 U
KK5512 KAPA 2G Fast HotStart PCR Kit with Mg-free buffer M,with dNTPs 250 U
KK5513 KAPA 2G Fast HotStart PCR Kit with Mg-free buffer M 250 U
KK5519 KAPA 2G Fast HotStart PCR Kit 2500 U
KK5523 KAPA 2G Fast HotStart PCR Kit 100 U
KK5530 KAPA 2G Fast HotStart PCR Kit with dNTPs 100 U
KK5601 KAPA 2G Fast HotStart ReadyMix PCR Kit 500 x 25 µl rxns
KK5603 KAPA 2G Fast HotStart ReadyMix PCR Kit 100 x 25 µl rxns
KK5801 KAPA 2G Fast Multiplex PCR Kit 100 x 25 µl rxns
KK5802 KAPA 2G Fast Multiplex PCR Kit 500 x 25 µl rxns

Kapa KK4601 通用型SYBR快速荧光定量PCR试剂盒

Kapa KK4601 通用型SYBR快速荧光定量PCR试剂盒

KAPA SYBR  FAST  suitable for qPCR, 2 ×, Universal

普通qPCR试剂,SYBR Green Ι 的存在抑制DNA聚合酶的扩增效率,无法保证DNA模板呈指数扩增,每次扩增产物与前一次相比小于2倍。KAPA SYBR FAST qPCR试剂盒含基因工程酶,耐受SYBR Green Ι 染料。因此反应体系中可以加入足够多的SYBR Green Ι ,显著降低达到阈值的循环数(Ct值),提高了信噪比、线性和灵敏度。

货号 产品名称 备注
KK4600 KAPA SYBR FAST Universal qPCR Kit 100 x 20 µl rxns(1ml)
KK4601 KAPA SYBR FAST Universal qPCR Kit 500 x 20 µl rxns(5ml)
KK4602 KAPA SYBR FAST Universal qPCR Kit 1000 x 20 µl rxns(10ml)
KK4603 KAPA SYBR FAST ABI Prism qPCR Kit 100 x 20 µl rxns(1ml)
KK4604 KAPA SYBR FAST ABI Prism qPCR Kit  500 x 20 µl rxns(5ml)
 KK4605  KAPA SYBR FAST ABI Prism qPCR Kit  1000 x 20 µl rxns(10ml)
 KK4606  KAPA SYBR FAST Bio-Rad iCycler qPCR Kit  100 x 20 µl rxns(1ml)
 KK4607  KAPA SYBR FAST Bio-Rad iCycler qPCR Kit  500 x 20 µl rxns(5ml)
 KK4608  KAPA SYBR FAST Bio-Rad iCycler qPCR Kit 1000 x 20 µl rxns(10ml)
 KK4609  KAPA SYBR FAST qPCR Kit for LightCycler480 100 x 20 µl rxns(1ml)
 KK4610  KAPA SYBR FAST qPCR Kit for LightCycler480   500 x 20 µl rxns(5ml)
 KK4611  KAPA SYBR FAST qPCR Kit for LightCycler480  1000 x 20 µl rxns(10ml)
 KK4617  KAPA SYBR FAST ABI Prism qPCR Kit  5000 x 20 µl rxns(50ml)
 KK4618  KAPA SYBR FAST Universal qPCR Kit  5000 x 20 µl rxns(50ml)
 KK4620  KAPA SYBR FAST ROX Low qPCR Kit  500 x 20 µl rxns(5ml)
产品优势
对SYBR green 染料的高耐受性
反应中更多的染料允许更高的信噪比
更多染料使Ct值更早出现,敏感性更好
工程酶具有更高的合成能力
产品性能
产品技术优点:
1)最高的反应效率使达到阈值的循环数最早出现,并且灵敏度极高;2)最高的荧光强度提高了信噪比;3)宽动态范围和很好的线性;4)反应快速,节约40-60%的反应时间;5)快速/标准反应程序结果无差异。
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KAPA 文库定量系列产品 (适用illumina平台)

KAPA 文库定量系列产品 (适用illumina平台)

KAPA Biosystems是一家美国生物公司,成立于2006年,其生产基地位于南非开普敦。KAPA Biosystems致力于研发、生产下一代PCR(Next-Generation PCR)所需的生物酶。目前其分子进化平台开发的产品已被广泛应用于DNA扩增、二代测序、分子诊断等领域。

Illumina平台的文库定量试剂

KAPA Library Quantification Kit——Illumina® Platforms

完全试剂盒包含标准DNA,引物混合物,qPCR混合液

完全试剂盒-规格:1mL qPCR混合液:

DNA Standards 1 – 6 (80 µL each),Primer Mix (0.2 mL)

KAPA SYBR® FAST qPCR Master Mix(1 mL)

KK4828 – 07960166001:Universal qPCR Master Mix

200 µL ROX High (50X) and ROX

Low (50X) supplied separay

完全试剂盒-规格:5 mL qPCR混合液:

DNA Standards 1 – 6 (80 µL each),Primer Mix (1 mL)

KAPA SYBR® FAST qPCR Master Mix(5 mL)

KK4824 – 07960140001:Universal qPCR Master Mix,200 µL ROX High (50X) and ROX

Low (50X) supplied separay

KK4835 – 07960204001:ABI Prism™ qPCR Master Mix

KK4844 – 07960255001:Bio-Rad iCycler™ qPCR Master Mix

KK4873 – 07960336001:ROX Low qPCR Master Mix

KK4854 – 07960298001:qPCR Master Mix optimized for LightCycler® 480

热销产品 KK4824

KAPA 文库定量试剂盒(illumina平台)特别设计用于Illumina测序文库的准确和可重复的定量,包含qPCR定量所需所有试剂。试剂盒含有新型KAPA SYBR FAST DNA聚合酶,保证效率的前提下,能定量平均片段长度高达1kb的Illumina文库

不完全试剂盒不含标准DNA

KK4923 – 07960441001:Universal qPCR Master Mix,200 µL ROX High (50X) and ROX

Low (50X) supplied separay

KK4933 – 07960484001:ABI Prism qPCR Master Mix

KK4943 – 07960522001:Bio-Rad iCycler qPCR Master Mix

KK4973 – 07960727001:ROX Low qPCR Master Mix

KK4953 – 07960573001:qPCR Master Mix optimized for LightCycler 480

搭配产品

引物和标准DNA

Kit with:Primer Mix (1 mL),DNA Standards (80 µL)

KK4808 – 07960085001:DNA Standards 1 – 6

标准DNA

Kits with:DNA Standards (80 µL)

KK4903 – 07960387001:DNA Standards 1 – 6

KK4905 – 07960409001:DNA Standards 0 – 6

稀释对照

Kit with:Dilution Control (80 µL)

KK4906 – 07960417001:DNA Standard 0

引物

KK4809 – 07960093001:Primer Mix (1 mL)

KAPA 文库扩增试剂盒 KAPA KK2611/KK2612

KAPA 文库扩增试剂盒 KAPA KK2611/KK2612

上海金畔生物代理销售Kapa biosystems品牌产品,欢迎新老客户访问Kapa biosystems官网或者咨询我们获取更多相关信息。

KAPA文库扩增试剂盒含有KAPA HiFi DNA聚合酶,是KAPA采用定向进化技术改造的一种新型工程酶,具有高保真性和稳定性。

KAPA HiFi是NGS文库扩增的优选聚合酶,它能够扩增复杂DNA,同时保证高保真、高效、更低PCR重复率和低偏差扩增。需要扩增的NGS文库构建时,有一些挑战性区域,比如GC和AT富含区域、启动子、低复杂性区,KAPA HiFi可以减少这些区域扩增的重复率,并提高覆盖度。

KAPA 文库扩增试剂盒 KAPA KK2611/KK2612 可用于

Illumina文库扩增(DNA和RNA测序)

ChIP DNA等珍贵样本的实时文库扩增

靶向捕获流程中的捕获前后的扩增

规格

兼容平台 Illumina HiSeq, MiSeq, NextSeq, and GAIIx

文库类型 DNA, RNA, and bisulfite-treated DNA

起始样本 任何需要扩增的NGS文库

测序应用 全基因组测序 全外显子测序 靶向测序(panel) RNA测序 ChIP测序 扩增子测序

KAPA Library Amplification Kit 文库扩增试剂盒

英文名称:KAPA Library Amplification Kit

中文名称:KAPA文库扩增试剂盒

品牌:KAPA

货号:KK2611/KK2612

规格:50 次/ 250 次

试剂盒内容:KAPA文库扩增试剂盒是一种聚合酶预混液(2X),包含KAPA HiFi HotStart DNA Polymerase(聚合酶),KAPA dNTPs(核苷酸),反应缓冲液和MgCl2,浓度2.5 mM。

试剂盒保存在-20˚C。

KAPA文库扩增性能

低的扩增偏差和重复率

 GC-和AT-富含区域的更高覆盖

行业*的保真度

订购信息

除了标准的文库扩增配方,KAPA HiFi还有实时PCR配方版,用于精确控制文库扩增。

尿嘧啶耐受变体,KAPA HiFi Uracil+ 可用于亚硫酸氢盐处理DNA的高保真、高效和低偏差扩增。

KAPA文库扩增试剂盒(illumina平台)也有优化的文库扩增引物混合物版。

品牌 货号 产品描述 规格
KAPA KK2611 KAPA文库扩增试剂盒,标准版 50次
KAPA KK2612 KAPA文库扩增试剂盒,标准版 250次
其他配方
KAPA KK2620 KAPA文库扩增试剂盒,含引物 50次
KAPA KK2621 KAPA文库扩增试剂盒,含引物 250次
KAPA KK2702 KAPA 实时PCR文库扩增试剂盒,含荧光标准品 250次
Product

Code

Product  Category Quantity 目录价
KK8230 KAPA  LTP文库构建(含文库扩增)试剂盒-Illumina 10个文库 2500
KK8231 KAPA  LTP文库构建(不含文库扩增)试剂盒-Illumina 10个文库 2400
KK8232 KAPA  LTP文库构建(含文库扩增)试剂盒-Illumina 50个文库 11000
KK8233 KAPA  LTP文库构建(不含文库扩增)试剂盒-Illumina 50个文库 10500
KK8234 KAPA  HTP文库构建(含文库扩增)试剂盒-Illumina 96个文库 18000
KK8235 KAPA  HTP文库构建(不含文库扩增)试剂盒-Illumina 96个文库 17000
KK8260 KAPA  TruSeq Adapter Kit, (Illumina, 20 μl each) 144 rxn 14000
KK2620 KAPA  Library Amplification Kit with Primers (50 x 50 μl reactions) 50rxn 1800
KK2621 KAPA  Library Amplification Kit with Primers (250 x 50 μl reactions) 250rxn 8000
KK2623 KAPA  Library Amplification Primer Kit (250 x 50 μl rxns) 250rxn 1500
KK8400 KAPA  Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions) 24  rxn 12200
KK8401 KAPA  Stranded RNA-Seq Library Preparation Kit (96 reactions) 96  rxn 38000
KK8420 KAPA  Stranded mRNA-Seq Library Preparation Kit (24 reactions) 24  rxn 14000
KK8421 KAPA  Stranded mRNA-Seq Library Preparation Kit (96 reactions) 96  rxn 44000
KK8500 KAPA  Hyper Prep Kit (8 reactions) 8  rxn 3100
KK8501 KAPA  Hyper Prep Kit, no amplification (8 reactions) 8  rxn 2970
KK8502 KAPA  Hyper Prep Kit (24 reactions) 24  rxn 8100
KK8503 KAPA  Hyper Prep Kit, no amplification (24 reactions) 24  rxn 7830
KK8504 KAPA  Hyper Prep Kit (96 reactions) 96  rxn 28100

KAPA产品目录及产品分类报价

KAPA产品目录及产品分类报价

KAPA Biosystems是一家美国生物公司,成立于2006年,其生产基地位于南非开普敦。KAPA Biosystems致力于研发、生产下一代PCR(Next-Generation PCR)所需的生物酶。目前其分子进化平台开发的产品已被广泛应用于DNA扩增、二代测序、分子诊断等领域。

上海金畔生物代理销售Kapa biosystems品牌产品,欢迎新老客户访问Kapa biosystems官网或者咨询我们获取更多相关信息。

HiFi高保真酶、HiFi高保真酶(二代测序)、长片段酶、与takara酶相对应的KAPA Extaq酶、HRM、SYBR荧光定量、PROBE荧光定量2G Robust酶及相关产品、2GFast酶及相关产品、血液直接扩增、DNA快速提取及扩增植物直扩、Mouse Genotyping相关产品
二代测序文库制备、二代测序文库定量:标准品+引物预混物、二代测序文库定量:标准品+引物预混物+qPCR试剂、二代测序最终文库定量产品人类基因组定量Kit(文库构建之前用,主要检测提取的DNA量)Taq相关产品、T4DNA连接酶、6碱基、8碱基、片段化酶、磁珠

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正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确价格货期以下单时确认为准。

第二代基因工程酶及相关产品

产品归类   KAPA Product Code Product Category   Quantity    目录价

HiFi高保真酶

KAPA KK2101 HiFi 100 units ¥700.00

KAPA KK2102 HiFi 250 units ¥1,600.00

KAPA KK2501 HiFi Hot Start 100 units ¥800.00

KAPA KK2502 HiFi Hot Start 250 units ¥1,800.00

KAPA KK2601 HiFi Hot Start 100 rxn ¥800.00

KAPA KK2602 HiFi Hot Start 500 rxn ¥3,500.00

HiFi高保真酶(二代测序)

KAPA KK2611 HiFi Hot Start for NGS 50 rxn ¥800.00

KAPA KK2612 HiFi Hot Start for NGS 250 rxn ¥3,500.00

KAPA KK2701 HiFi Hot Start for NGS 50 rxn ¥2,400.00

KAPA KK2702 HiFi Hot Start for NGS 250 rxn ¥10,000.00

KAPA KK2801 KAPA HiFi  HS Uracil +ready Mix 50 x 50µL reactions ¥3,000.00

KAPA KK2802 KAPA HiFi  HS Uracil +ready Mix 250 x 50µL reactions ¥14,000.00

长片段酶 KAPA KK3006 Long Range 100 units ¥800.00

KAPA KK3005 Long Range 250 units ¥1,600.00

KAPA KK3501 Long Range Hot Start 100 units ¥900.00

KAPA KK3502 Long Range Hot Start 250 units ¥1,800.00

KAPA KK3503 Long Range Hot Start 500 units ¥3,000.00

KAPA KK3601 Long Range Hot StartReady Mix with DYE (100 x 25µL rxn) (100 x 25µL rxn) ¥1,100.00

KAPA KK3602 Long Range Hot StartReady Mix with DYE (500 x 25µL rxn) (500 x 25µL rxn) ¥4,500.00

与takara酶相对应的KAPA Extaq酶

KAPA KK3009 KAPATaq Extra(针对takara ExTaq) 250units ¥400.00

KAPA KK3505 KAPATaq Extra Hot Start(针对takara ExTaq) 250units ¥520.00

KAPA KK3604 KAPATaq Extra Hot Start Ready Mix with DYE (针对takara ExTaq) (100 x 25µL rxn) ¥400.00

KAPA KK3605 KAPATaq Extra Hot StartReady Mix with DYE(针对takara ExTaq) (500 x 25µL rxn) ¥1,800.00

KAPA KK3606 KAPATaq Extra Hot Start Ready Mix with DYE (200 x 25µL rxn) ¥800.00

HRM KAPA KK4201 KAPA HRM FAST 1ml ¥700.00

KAPA KK4202 KAPA HRM FAST 5ml ¥3,200.00

KAPA KK4203 KAPA HRM FAST 10ml ¥6,000.00

SYBR荧光定量

KAPA KK4600 SYBR® Fast通用型 1 ml ¥400.00

KAPA KK4601 SYBR® Fast通用型 5 ml ¥1,800.00

KAPA KK4602 SYBR® Fast通用型 10 ml ¥3,500.00

KAPA KK4618 SYBR® Fast通用型 50 ml ¥17,000.00

KAPA KK4603 SYBR® Fast(ABI Prism) 1 ml ¥400.00

KAPA KK4604 SYBR® Fast(ABI Prism) 5 ml ¥1,800.00

KAPA KK4605 SYBR® Fast(ABI Prism) 10 ml ¥3,500.00

KAPA KK4617 SYBR® Fast(ABI Prism) 50ml ¥17,000.00

KAPA KK4620 KK4620KAPASYBRFASTROXLow(5ml=500x20ulrxns) 500*20ul rxns ¥1,800.00

KAPA KK4606 SYBR® Fast(伯乐型) 1 ml ¥400.00

KAPA KK4607 SYBR® Fast(伯乐型) 5 ml ¥1,800.00

KAPA KK4608 SYBR® Fast(伯乐型) 10 ml ¥3,500.00

KAPA KK4609 SYBR® Fast (Roche LC 480) 1 ml ¥400.00

KAPA KK4610 SYBR® Fast(Roche LC 480) 5 ml ¥1,800.00

KAPA KK4611 SYBR® Fast(Roche LC 480) 10 ml ¥3,500.00

KAPA KK4650 1-step qRT-PCR Kit(通用型) 1ml ¥1,000.00

KAPA KK4651 1-step qRT-PCR Kit(通用型) 5ml ¥4,500.00

KAPA KK4652 1-step qRT-PCR Kit(通用型) 10ml ¥8,000.00

KAPA KK4660 1-step qRT-PCR Kit(ABI Prism) 1ml ¥1,000.00

KAPA KK4661 1-step qRT-PCR Kit(ABI Prism) 5ml ¥4,500.00

KAPA KK4662 1-step qRT-PCR Kit(ABI Prism) 10ml ¥8,000.00

KAPA KK4670 1-step qRT-PCR Kit(伯乐型) 1ml ¥1,000.00

KAPA KK4671 1-step qRT-PCR Kit(伯乐型) 5ml ¥4,500.00

KAPA KK4672 1-step qRT-PCR Kit(伯乐型) 10ml ¥8,000.00

KAPA KK4680 1-step qRT-PCR Kit(Roche LC 480) 1ml ¥1,000.00

KAPA KK4681 1-step qRT-PCR Kit(Roche LC 480) 5ml ¥4,500.00

KAPA KK4682 1-step qRT-PCR Kit(Roche LC 480) 10ml ¥8,000.00

PROBE荧光定量 KAPA KK4701 PROBE FAST(通用型) 1 ml ¥400.00

KAPA KK4702 PROBE FAST(通用型) 5 ml ¥1,800.00

KAPA KK4703 PROBE FAST(通用型) 10 ml ¥3,500.00

KAPA KK4705 PROBE FAST(ABI Prism) 1 ml ¥400.00

KAPA KK4706 PROBE FAST(ABI Prism) 5 ml ¥1,800.00

KAPA KK4707 PROBE FAST(ABI Prism) 10 ml ¥3,500.00

KAPA KK4709 PROBE FAST(伯乐型) 1 ml ¥400.00

KAPA KK4710 PROBE FAST(伯乐型) 5 ml ¥1,800.00

KAPA KK4711 PROBE FAST(伯乐型) 10 ml ¥3,500.00

KAPA KK4714 PROBE FAST(ABI Prism,定制50ml体系) 50 ¥17,000.00

KAPA KK4715 PROBE FAST(通用型,定制50ml体系) 50 ¥17,000.00

KAPA KK4716 PROBE FAST (ROX Low Mastermix) 1ml ¥400.00

KAPA KK4717 PROBE FAST(ROX Low Mastermix) 5 ml ¥1,800.00

KAPA KK4718 PROBE FAST(ROX Low Mastermix) 10 ml ¥3,500.00

KAPA KK4719 PROBE FAST(ROX Low Mastermix) 50 ml ¥17,000.00

KAPA KK4722 KK4722KAPAPROBEFASTUnivrsalqPCRKitwithdUTP 500x20ul rxns ¥1,800.00

KAPA KK4752 PROBE FAST(1-step qRT-PCR Probe Universal) 5 ml ¥4,500.00

KAPA KK4300 通用型PROBE FORCE定量试剂 1ml ¥700.00

KAPA KK4301 通用型PROBE FORCE定量试剂 5ml ¥3,500.00

KAPA KK4302 通用型PROBE FORCE定量试剂 10ml ¥6,000.00

KAPA KK4303 通用型PROBE FORCE定量试剂 50ml ¥26,000.00

2G Robust酶及相关产品

KAPA KK5023 2G Robust 100 units ¥400.00

KAPA KK5024 2G Robust 250 units ¥900.00

KAPA KK5004 2G Robust(含dNTPs) 100units ¥400.00

KAPA KK5005 2G Robust(含dNTPs) 250 units ¥900.00

KAPA kk5522 2G Robust Hot Start 100units ¥600.00

KAPA KK5515 2G Robust Hot Start 250 units ¥1,400.00

KAPA KK5517 2G Robust Hot Start 500 units ¥2,400.00

KAPA KK5525 2G Robust Hot Start 2500units ¥9,000.00

KAPA KK5532 2G Robust Hot Start(含dNTPs) 100 units ¥700.00

KAPA KK5516 2G Robust Hot Start(含dNTPs) 250 units ¥1,500.00

KAPA KK5518 2G Robust Hot Start(含dNTPs) 500units ¥2,800.00

KAPA KK5701 2G Robust Hot Start Readymix 100rxn ¥700.00

KAPA KK5702 2G Robust Hot Start Readymix 500 rxn ¥2,600.00

2GFast酶及相关产品

KAPA KK5020 2G Fast 100 units ¥400.00

KAPA KK5021 2G Fast 250 units ¥900.00

KAPA KK5008 2G Fast(含dNTPs) 100 units ¥500.00

KAPA KK5009 2G Fast(含dNTPs) 250 units ¥1,100.00

KAPA KK5101 2G Fast Ready Mix+ Dye 100rxn ¥400.00

KAPA KK5102 2G Fast Ready Mix +Dye 500 rxn ¥1,400.00

KAPA KK5523 2G Fast Hot Start 100 units ¥600.00

KAPA KK5503 2G Fast Hot Start 250 units ¥1,300.00

KAPA KK5501 2G Fast Hot Start 500 units ¥2,400.00

KAPA KK5519 2G Fast Hot Start 2500 units ¥7,000.00

KAPA KK5530 2G Fast Hot Start(含dNTPs) 100 units ¥700.00

KAPA KK5502 2G Fast Hot Start(含dNTPs) 250uinits ¥1,500.00

KAPA KK5500 2G Fast Hot Start(含dNTPs) 500 units ¥2,600.00

KAPA KK5513 2G Fast Hot Start(Buffer无Mg2+) 250 units ¥1,400.00

KAPA KK5511 2G Fast Hot Start(Buffer无Mg2+) 500 units ¥2,400.00

KAPA kk5512 2G Fast Hot Start(含dNTPs,Buffer无Mg2+) 250 units ¥1,500.00

KAPA KK5510 2G Fast Hot Start(含dNTPs,Buffer无Mg2+) 500 units ¥2,800.00

KAPA KK5603 2G Fast Hot Start ReadyMix 100 rxn ¥400.00

KAPA KK5601 2G Fast Hot Start ReadyMix 500 rxn ¥1,400.00

KAPA KK5801 2G Fast Hot Start 100 rxn ¥500.00

KAPA KK5802 2G Fast Hot Start 500 rxn ¥1,900.00

血液直接扩增

KAPA KK7002 Blood PCR Kit A 500 rxn ¥2,500.00

KAPA KK7003 Blood PCR Kit B 500 rxn ¥2,500.00

KAPA KK7004 Blood PCR Kit A 1000 rxn ¥4,500.00

KAPA KK7005 Blood PCR Kit B 1000 rxn ¥4,500.00

DNA快速提取及扩增 KAPA KK7100 Express Extract 50 rxn ¥400.00

KAPA KK7101 Express Extract 100 rxn ¥700.00

KAPA KK7102 Express Extract 250 rxn ¥1,500.00

KAPA KK7103 Express Extract 500 rxn ¥2,600.00

KAPA KK7151 Express Extract+ 2GR HS 100 rxn combo ¥1,200.00

KAPA KK7152 Express Extract+ 2GR HS 500 rxn combo ¥4,600.00

植物直扩

KAPA KK7251 KAPA3G Plant PCR 250 units ¥3,000.00

KAPA KK7252 KAPA3G Plant PCR 500 units ¥5,400.00

Mouse Genotyping相关产品 KAPA KK7301 Mouse Genotyping Kit 100 rxn ¥800.00

KAPA KK7302 Mouse Genotyping Kit 500 rxn ¥4,000.00

KAPA KK7351 HS  Mouse Genotyping Kit 100 rxn ¥1,200.00

KAPA KK7352 HS  Mouse Genotyping Kit 500 rxn ¥5,000.00

KAPA KK5620 2G Fast HotStart Genotyping PCR Kit 100 rxn ¥500.00

KAPA KK5621 2G Fast HotStart Genotyping PCR Kit 500 rxn ¥2,000.00

二代测序文库制备

KAPA KK8230 KAPA LTP文库构建(含文库扩增)试剂盒-Illumina 10个文库 ¥2,500.00

KAPA KK8231 KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina 10个文库 ¥2,400.00

KAPA KK8232 KAPA LTP文库构建(含文库扩增)试剂盒-Illumina 50个文库 ¥11,000.00

KAPA KK8233 KAPA LTP文库构建(不含文库扩增)试剂盒-Illumina 50个文库 ¥10,500.00

KAPA KK8234 KAPA HTP文库构建(含文库扩增)试剂盒-Illumina 96个文库 ¥18,000.00

KAPA KK8235 KAPA HTP文库构建(不含文库扩增)试剂盒-Illumina 96个文库 ¥17,000.00

KAPA KK8260 KAPA TruSeq Adapter Kit, (Illumina, 20 μl each) 144 rxn ¥14,000.00

KAPA KK2620 KAPA Library Amplification Kit with Primers (50 x 50 µl reactions) 50rxn ¥1,800.00

KAPA KK2621 KAPA Library Amplification Kit with Primers (250 x 50 µl reactions) 250rxn ¥5,000.00

KAPA KK2623 KAPA Library Amplification Primer Kit (250 x 50 μl rxns) 250rxn ¥1,500.00

KAPA KK8400 KAPA Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions) 24 rxn ¥13,000.00

KAPA KK8401 KAPA Stranded RNA-Seq Library Preparation Kit (96 reactions) 96 rxn ¥40,000.00

KAPA KK8420 KAPA Stranded mRNA-Seq Library Preparation Kit (24 reactions) 24 rxn ¥15,000.00

KAPA KK8421 KAPA Stranded mRNA-Seq Library Preparation Kit (96 reactions) 96 rxn ¥47,000.00

KAPA KK8483 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (24rxns) 24rxn ¥28,000.00

KAPA KK8484 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (96rxns) 96rxn ¥98,000.00

KAPA KK8500 KAPA Hyper Prep Kit (8 reactions) 8 rxn ¥3,100.00

KAPA KK8501 KAPA Hyper Prep Kit, no amplification (8 reactions) 8 rxn ¥2,970.00

KAPA KK8502 KAPA Hyper Prep Kit (24 reactions) 24 rxn ¥8,100.00

KAPA KK8503 KAPA Hyper Prep Kit, no amplification (24 reactions) 24 rxn ¥7,830.00

KAPA KK8504 KAPA Hyper Prep Kit (96 reactions) 96 rxn ¥28,100.00

KAPA KK8505 KAPA Hyper Prep Kit, no amplification (96 reactions) 96 rxn ¥27,000.00

KAPA KK8510 KAPA Hyper Plus Kit (Illumina,8 rxns) 8 rxn ¥3,500.00

KAPA KK8511 KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns) 8 rxn ¥3,370.00

KAPA KK8512 KAPA Hyper Plus Kit (Illumina,24 rxns) 24 rxn ¥9,300.00

KAPA KK8513 KAPA Hyper Plus Kit, PCR-free (Illumina,24rxns) 24 rxn ¥9,030.00

KAPA KK8514 KAPA Hyper Plus Kit (Illumina,96 rxns) 96 rxn ¥32,900.00

KAPA KK8515 KAPA Hyper Plus Kit, PCR-free (Illumina,96 rxns) 96 rxn ¥31,800.00

KAPA KK8300 Ion Torrent  Library Preparation Kit (8 reactions)  8 rxn ¥2,520.00

KAPA KK8301 Ion Torrent   Library Preparation Kit (48 reactions) 48 rxn ¥13,860.00

KAPA KK8310 Ion Torrent Library Preparation Kit, no amplification (8 reactions)  8 rxn ¥2,280.00

KAPA KK8311 Ion TorrentLibrary Preparation Kit, no amplification (48 reactions) 48 rxn ¥12,420.00

KAPA KK8330 Adapter Kit, non-Barcoded (8 reactions    Ion Torrent 8 rxn ¥1,200.00

KAPA KK8331 Adapter Kit, Barcodes 1 – 8 (48 reactions)   Ion Torrent 48 rxn ¥7,500.00

KAPA KK8332 Adapter Kit, Barcodes 9 – 16 (48 reactions)   Ion Torrent 48 rxn ¥7,500.00

KAPA KK8333 Adapter Kit, Barcodes 17 – 24 (48 reactions)   Ion Torrent 48 rxn ¥7,500.00

二代测序文库定量:标准品+引物预混物

KAPA KK4800 DNA Quant Kit   Roche 454 FLX Each ¥6,000.00

KAPA KK4801 Primer Premix  Roche 454 FLX Each ¥3,000.00

KAPA KK4802 DNA Quant Kit  Roche 454 Titanium Each ¥6,000.00

KAPA KK4803 Primer Premix Roche 454 Titanium Each ¥3,000.00

KAPA KK4804 DNA Quant Kit  Illumina Genome Analyzer Each ¥6,000.00

KAPA KK4805 Primer Premix  Illumina Genome Analyzer Each ¥3,000.00

KAPA KK4806 DNA Quant Kit  ABI SOLiD Each ¥6,000.00

KAPA KK4807 Primer Premix  ABI SOLiD Each ¥3,000.00

KAPA KK4808 DNA Quant Kit   Illumina GA Revised Primers Each ¥6,000.00

KAPA KK4809 Primer Premix  Illumina GA Revised Primers Each ¥3,000.00

KAPA KK4812 DNA Quant Kit – Ion Torrent Each ¥6,000.00

KAPA KK4813 DNA Quantification Primer Premix –  Ion Torrent Each ¥3,000.00

二代测序文库定量:标准品+引物预混物+qPCR试剂

KAPA KK4900 Library Quantification DNA Standards (454 FLX/Lib-A) 6 x 80 µl ¥4,000.00

KAPA KK4902 Library Quantification DNA Standards (SOLiD) 6 x 80 µl ¥4,000.00

KAPA KK4903 Library Quantification DNA Standards (Illumina) 6 x 80 µl ¥4,000.00

KAPA KK4904 Library Quantification DNA Standards (Ion Torrent) 6 x 80 µl ¥4,000.00

KAPA KK4923 KK4923Library Quant Primers+KSF(IIIumina/Uni) IIIumina/Uni ¥4,000.00

KAPA KK4924 kk4924 Library Quant primers+KSF(10mTorrent/uni) 10mTorrent/uni ¥5,000.00

二代测序最终文库定量产品

KAPA KK4820 Library  Quant Kit,Roche 454 FLX-SYBR Fast Universal Each ¥7,000.00

KAPA KK4821 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Universal Each ¥7,000.00

KAPA KK4824 Library  Quant Kit,Illumina GA revised primers -SYBR Fast Universal Each ¥7,000.00

KAPA KK4823 Library  Quant Kit,ABI SOLiD-SYBR Fast Universal Each ¥7,000.00

KAPA KK4830 Library  Quant Kit,Roche 454 FLX-SYBR Fast ABI Prism Each ¥7,000.00

KAPA KK4831 Library  Quant Kit,Roche 454 Titanium-SYBR Fast ABI Prism Each ¥7,000.00

KAPA KK4835 Library  Quant Kit,Illumina GA revised primers -SYBR Fast ABI Prism Each ¥7,000.00

KAPA KK4833 Library  Quant Kit,ABI SOLiD-SYBR Fast ABI Prism Each ¥7,000.00

KAPA KK4840 Library  Quant Kit,Roche 454 FLX-SYBR Fast Bio-Rad iCycler Each ¥7,000.00

KAPA KK4841 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Bio-Rad iCycler Each ¥7,000.00

KAPA KK4844 Library  Quant Kit,Illumina GA revised primers-SYBR Fast Bio-Rad iCycler Each ¥7,000.00

KAPA KK4843 Library  Quant Kit,ABI SOLiD-SYBR Fast Bio-Rad iCycler Each ¥7,000.00

KAPA KK4850 Library  Quant Kit,Roche 454 FLX-SYBR Fast Roche Light Cycler480 Each ¥7,000.00

KAPA KK4851 Library  Quant Kit,Roche 454 Titanium-SYBR Fast Roche Light Cycler 480 Each ¥7,000.00

KAPA KK4854 Library  Quant Kit,Illumina GA revised primers-SYBR Fast Roche Light Cycler480 Each ¥7,000.00

KAPA KK4853 Library  Quant Kit,ABI SOLiD-SYBR Fast Roche Light Cycler480 Each ¥7,000.00

KAPA KK4834 Library  Quant Kit,Illumina GA ABI Prism Custom Kit- Sanger Each ¥7,000.00

KAPA KK4827 Library  Quant Kit,Ion Torrent PGM universal Each ¥7,000.00

KAPA KK4838 Library  Quant Kit,Ion Torrent PGM ABI Prism Each ¥7,000.00

KAPA KK4847 Library  Quant Kit,Ion Torrent PGM  Bio-Rad iCycler Each ¥7,000.00

KAPA KK4857 Library  Quant Kit,Ion Torrent PGM  Roche Light Cycler480 Each ¥7,000.00

KAPA KK4873 KK4873Library Quant Kit (Illumina/ROX Low) 500rxn ¥7,000.00

人类基因组定量Kit(文库构建之前用,主要检测提取的DNA量)

KAPA KK4960 hg DNA Quant & QC kit with SYBR Universal qPCR Mastermix Each ¥8,000.00

KAPA KK4961 hg DNA Quant & QC kit with SYBR ABI Prism qPCR Mastermix Each ¥8,000.00

KAPA KK4962 hg DNA Quant & QC kit with SYBR Bio-Rad qPCR Mastermix Each ¥8,000.00

KAPA KK4963 hg DNA Quant & QC kit with SYBR Light Cycler 480 qPCR Mastermix Each ¥8,000.00

KAPA KK4964 hg DNA Quant & QC DNA Standards Kit Each ¥6,000.00

KAPA KK4965 hg DNA Quant & QC and 41 bp Primer Kit Each ¥1,500.00

KAPA KK4966 hg DNA Quant & QC and 129 bp Primer Kit Each ¥1,500.00

KAPA KK4967 hg DNA Quant & QC and 305 bp Primer Kit Each ¥1,500.00

KAPA KK4969 hg DNA Quant & QC Kit with ROX Low qPCR Mastermix Each ¥8,000.00

KAPA产品归类 KAPA Product Code Product Category Quantity

Taq相关产品

KAPA KK1014 Taq 酶 250 units ¥380.00

KAPA KK1015 Taq 酶 500 units ¥700.00

KAPA BK1000 Taq 酶 2500 units ¥2,500.00

KAPA BK1002 Taq 酶 5000 units ¥3,800.00

KAPA KK1008 Taq 酶(含dNTPs) 250 units ¥450.00

KAPA KK1016 Taq 酶(含dNTPs) 500 units ¥800.00

KAPA BK1001 Taq 酶(含dNTPs) 2500 units ¥3,500.00

KAPA BK1003 Taq 酶(含dNTPs) 5000 units ¥5,500.00

KAPA KK1006 Taq 酶 ReadyMix 250×50µl反应 ¥900.00

KAPA KK1020 Taq 酶Buffer w/ loading dye 250 units ¥380.00

KAPA KK1022 Taq 酶Buffer w/ loading dye 500 units ¥700.00

KAPA BK1004 Taq 酶Buffer w/ loading dye 2500 units ¥2,500.00

KAPA BK1006 Taq 酶Buffer w/ loading dye 5000 units ¥3,800.00

KAPA KK1021 Taq 酶Buffer w/ loading dye(含dNTPs) 250 units ¥450.00

KAPA KK1023 Taq 酶Buffer w/ loading dye(含dNTPs) 500 units ¥800.00

KAPA BK1005 Taq 酶Buffer w/ loading dye(含dNTPs) 2500 units ¥3,500.00

KAPA BK1007 Taq 酶Buffer w/ loading dye(含dNTPs) 5000 units ¥5,500.00

KAPA KK1024 Taq 酶ReadyMix w/loading dye 250×50µl反应 ¥900.00

KAPA KK1508 热启动Taq酶 250 units ¥800.00

KAPA KK1510 热启动Taq酶 500 units ¥1,200.00

KAPA KK1513 热启动Taq酶 2500 units ¥5,000.00

KAPA KK1509 热启动Taq酶(含dNTPs) 250 units ¥900.00

KAPA KK1511 热启动Taq酶(含dNTPs) 500 units ¥1,500.00

KAPA KK1512 热启动Taq酶without buffers 2500 units ¥5,000.00

T4DNA连接酶

KAPA KK6010 T4 DNA Ligase,5units/µL (Weiss Units) 250 units ¥600.00

KAPA KK6011 T4 DNA Ligase,5units/µL (Weiss Units) 1250 units ¥2,500.00

KAPA KK6110 T4 DNA Ligase,Rapid Ligation Kit 50 rxn ¥1,400.00

KAPA KK6111 T4 DNA Ligase,Rapid Ligation Kit 150 rxn ¥3,800.00

KAPA 产品归类 KAPA Product Code Product Category Quantity

单一品种

KAPA PKA1001 PKA1001 Truseq Adapter index1 30uM*20ul ¥400.00

KAPA PKA1002 PKA1002 Truseq Adapter  index2 30uM*20ul ¥400.00

KAPA PKA1003 PKA1003 Truseq Adapter index3 30uM*20ul ¥400.00

KAPA PKA1004 PKA1004 Truseq Adapter index4 30uM*20ul ¥400.00

KAPA PKA1005 PKA1005 Truseq Adapter index5 30uM*20ul ¥400.00

KAPA PKA1006 PKA1006 Truseq Adapter index6 30uM*20ul ¥400.00

KAPA PKA1007 PKA1007 Truseq Adapter index7 30uM*20ul ¥400.00

KAPA PKA1008 PKA1008 Truseq Adapter  index8 30uM*20ul ¥400.00

KAPA PKA1009 PKA1009 Truseq Adapter index9 30uM*20ul ¥400.00

KAPA PKA1010 PKA1010 Truseq Adapter  index10 30uM*20ul ¥400.00

KAPA PKA1011 PKA1011 Truseq Adapter index11 30uM*20ul ¥400.00

KAPA PKA1012 PKA1012 Truseq Adapter index12 30uM*20ul ¥400.00

KAPA PKR2001 PKR2001 PKR Truseq Adapter Kit index1-12 30uMx20ul ¥4,800.00

KAPA PKR2012 PKR2012 (13-27) 30uMx20ul ¥4,800.00

KAPA PKR2015 PKR2015 (1-24) 10μl ¥3,750.00

KAPA PKR2016 PKR2016 (25-48) 10μl ¥3,750.00

KAPA PKR2017 PKR2017 (49-72) 10μl ¥3,750.00

KAPA PKR2018 PKR2018 (73-96) 10μl ¥3,750.00

KAPA片段化酶

KAPA KK8600 KK8600 Library Preparation – Illumina    8 rxn ¥560.00

KAPA KK8601 KK8601 Library Preparation – Illumina      24 rxn ¥1,620.00

KAPA KK8602 KK8602 Library Preparation – Illumina      96 rxn ¥6,300.00

KAPA磁珠

KAPA KK8000 KK8000 KAPA Pure Beads (5mL)  5ml ¥2,200.00

KAPA KK8001 KK8001 KAPA Pure Beads (30mL) 30ml ¥6,600.00

KAPA KK8002 KK8002 KAPA Pure Beads (60mL) 60ml ¥11,000.00​

KAPA KK4824-Library Quantification Kit 文库定量试剂

KAPA KK4824-Library Quantification Kit 文库定量试剂

适用Illumina文库

KAPA Biosystems是一家美国生物公司,成立于2006年,其生产基地位于南非开普敦。KAPA Biosystems致力于研发、生产下一代PCR(Next-Generation PCR)所需的生物酶。目前其分子进化平台开发的产品已被广泛应用于DNA扩增、二代测序、分子诊断等领域。

KAPA KK4824-Library Quantification Kit 文库定量试剂

英文名称:KAPA Library Quantification Kits for Illumina platforms

中文名称:illumina测序文库定量试剂盒

KAPA 文库定量试剂盒(illumina平台)特别设计用于Illumina测序文库的准确和可重复的定量,包含qPCR定量所需所有试剂。试剂盒含有新型KAPA SYBR FAST DNA聚合酶,保证效率的前提下,能定量平均片段长度高达1kb的Illumina文库。

产品特点

试剂盒适用序列GC含量范围宽泛,平均片段长度可达1 kb。

除了NGS文库定量之外,该试剂盒还可用于检测Illumina文库制备过程中的污染情况。

文库定量分析包括重复的移液步骤,容易自动化。

经过严格测试,以确保zui小的批次间差异。

产品应用和兼容性

KAPA Library定量试剂盒(illumina平台)特别设计用于Illumina测序文库的准确和可重复的定量。适用文库不限类型、构建方式和illumina测序仪型号,只要浓度> 0.0002 pM,并且含有与试剂盒中引物互补的序列(Illumina P5 and P7流动池寡核苷酸序列),都可以使用该试剂盒进行定量。

KAPA Library Quantification Kits for Illumina platforms are suitable for the quantification of any NGS library (prepared for Illumina sequencing) which contains the P5 and P7 flow cell sequence motifs. Libraries constructed using full-length universal or indexed TruSeq™ adapters can be quantified after adapter ligation. Libraries prepared with partial or stem-loop adapters can only be quantified with this kit after library amplification, during which indexes (and flow cell sequences) are added. To ensure compatibility, compare the adapter sequences used during library preparation with the library quantification primer sequences in Product Description.

Ensure that the correct KAPA SYBR FAST qPCR Master Mix is used, in accordance with the reference dye requirements (if any) of the qPCR instrument to be used for library quantification.

产品组份

 

 

 

 

 

 

 

 

适用于Illumina平台的KAPA Library定量试剂盒包含所有含P5和P7流动池序列的Illumina文库进行qPCR定量所需的完整试剂。

完全试剂盒包含标准DNA,引物混合物,qPCR混合液

试剂盒包含:

•文库定量DNA标准品 1 – 6 (a 10-fold dilution series of a linear, 452 bp template)

•文库定量引物Primer Premix (10X), 引物序列:

Primer 1: 5′-AAT GAT ACG GCG ACC ACC GA-3′

Primer 2: 5′-CAA GCA GAA GAC GGC ATA CGA-3′

•KAPA SYBR® FAST qPCR Master Mix (2X),各种passive reference染料。

该试剂盒含有新型KAPA SYBR FAST DNA聚合酶,通过定向进化过程设计用于高性能SYBR Green I型qPCR。改造的聚合酶以相似的效率扩增不同DNA片段的能力使得能够使用通用标准来可靠地量化平均片段长度高达1kb的所有Illumina文库,而不管文库类型或GC含量如何。

KAPA SYBR FAST是一种抗体介导的热启动DNA聚合酶制剂。

使用方法

1. 使用KAPA SYBR FAST qPCR Master Mix和引物(靶向Illumina P5和P7流动池序列)

通过qPCR法扩增DNA标准品(6个,预稀释)和稀释的文库样品来进行文库定量。

2. 绘制每个DNA标准品的平均Cq值与log10(浓度:pM)的关系曲线,以产生标准曲线。使用定量对照标准曲线计算稀释的文库样品的浓度。

注意:高通量NGS管路强烈建议使用自动化液体处理系统。有关更多信息,请参阅KAPA库量化技术指南。

Library quantification is performed by amplifying the set of six pre-diluted DNA Standards and diluted library samples by qPCR, using the KAPA SYBR FAST qPCR Master Mix and primers targeting the Illumina P5 and P7 flow cell oligo sequences. The average Cq score for each DNA Standard is plotted against log10(concentration in pM) to generate a standard curve. The concentrations of diluted library samples are then calculated against the standard curve, using absolute quantification.

KAPA HiFi HotStart ReadyMix 高保真DNA聚合酶预混液

KAPA HiFi HotStart ReadyMix 高保真DNA聚合酶预混液

英文名称:KAPA HiFi HotStart ReadyMix

中文名称:KAPA HiFi高保真热启动DNA聚合酶预混液

KAPA  KK2611    KK2612   文库扩增试剂盒

KAPA HiFi HotStart ReadyMix是一种DNA扩增预混液,包含引物和模板之外扩增完全反应必需成分。适用PCR、NGS高通量测序文库等的扩增。预混液所含KAPA HiFi DNA Polymerase是新型改造B族DNA聚合酶,错误率比野生型Taq DNA聚合酶低约100倍,成功率和产率比野生型B族proofreading活性的DNA聚合酶更高。

HiFi 热启动高保真DNA聚合酶应用

用于常规测序的PCR(直接测序或克隆PCR产物的测序)

新一代测序文库扩增(与KAPA文库扩增方案配合使用时)

扩增片段DNA用于克隆和蛋白质表达或基因组表征

定点突变

KAPA HiFi DNA聚合酶扩增特点

高DNA亲和力,无需辅助蛋白和DNA结合结构域

高持续合成能力

长片段扩增能力

选择失活,低非特异性扩增

KAPA HiFi HotStart DNA聚合酶是一种新型B家族DNA聚合酶,经过工程改造后对DNA的亲和力增加,不需要辅助蛋白或DNA结合域。与野生型B家族DNA聚合酶相比,该酶固有的高持续合成能力可显著提高合成产量,速度和灵敏度。此外,扩增长片段以及富含GC和AT片段的能力显著提高。该酶与特有抗体结合,*次变性步骤之前可以保持失活状态。这可以防止反应过程中的非特异性扩增,提高灵敏度,并提高反应效率。

组份(除引物和模板的扩增反应液)

DNA聚合酶+缓冲液混合

— DNA聚合酶:0.5U /25μL反应

— 缓冲液:dNTPs(每种dNTP 0.3mM,1X),MgCl2(2.5mM,1X )和稳定剂

KAPA HiFi高保真DNA聚合酶技术参数

聚合酶种类:改造B族DNA聚合酶

聚合酶活性:KAPA HiFi HotStart DNA聚合酶具有5’g3’聚合酶和3’g5’核酸外切酶(proofreading)活性,但没有5’g3’核酸外切酶活性。

扩增片段长度:可从基因组DNA中扩增高达15kb的目标或从较不复杂的目标扩增20kb。

KAPA HiFi高保真DNA聚合酶预混液设计用于各种目标和大小片段的常规高保真PCR。它的错误率比野生型Taq DNA聚合酶低约100倍,成功率和产率比野生型B家族(proofreading)DNA聚合酶更高。另外,KAPA HiFi HotStart比野生型B家族DNA聚合酶需要更短的反应时间。 KAPA HiFi HotStart DNA聚合酶具有5’g3’聚合酶和3’g5’核酸外切酶(proofreading)活性,但没有5’g3’核酸外切酶活性。

Product Line KAPA Code Product Chinese Name Product Description Spec Rxn
HiFi & Lib Amp KK2101 KK2101 KAPA HiFi高保真酶+dNTPs (100反应) KAPA HiFi+dNTPs (100rxn) 100*50μL rxn/200*25μL rxn 100
HiFi & Lib Amp KK2102 KK2102 KAPA HiFi高保真酶+dNTPs (250反应) KAPA HiFi+dNTPs (250rxn) 250*50μL rxn/500*25μL rxn 250
HiFi & Lib Amp KK2501 KK2501 KAPA 热启动HiFi高保真酶+dNTPs (100反应) KAPA HiFi HotStart+dNTPs (100rxn) 100*50μL rxn/200*25μL rxn 100
HiFi & Lib Amp KK2502 KK2502 KAPA 热启动HiFi高保真酶+dNTPs (250反应) KAPA HiFi HotStart+dNTPs (250rxn) 250*50μL rxn/500*25μL rxn 250
HiFi & Lib Amp KK2601 KK2601 KAPA 热启动HiFi高保真酶预混液 (1.25mL) KAPA HiFi HotStart ReadyMix Kit (1.25mL) 1.25mL/50*50μL rxn/100*25μL rxn 50
HiFi & Lib Amp KK2602 KK2602 KAPA 热启动HiFi高保真酶预混液 (6.25mL) KAPA HiFi HotStart ReadyMix Kit (6.25mL) 6.25mL/250*50μL rxn/500*25μL rxn 250
HiFi & Lib Amp KK2631 KK2631 KAPA 热启动HiFi高保真酶预混液 (50mL) KAPA HiFi HotStart ReadyMix Kit (50mL) 50mL/2000*50μL rxn/4000*25μL rxn 2000
HiFi & Lib Amp KK2625 KK2625 KAPA 热启动HiFi高保真酶预混液 (50mL) v2 KAPA HiFi HotStart ReadyMix Kit (50mL) v2 50mL/2000*50μL rxn/4000*25μL rxn 2000
HiFi & Lib Amp KK2801 KK2801 KAPA Uracil+热启动HiFi高保真酶预混液 (1.25mL) KAPA HiFi HotStart Uracil+ ReadyMix Kit (1.25mL) 1.25mL/50*50μL rxn/100*25μL rxn 50
HiFi & Lib Amp KK2802 KK2802 KAPA Uracil+热启动HiFi高保真酶预混液 (6.25mL) KAPA HiFi HotStart Uracil+ ReadyMix Kit (6.25mL) 6.25mL/250*50μL rxn/500*25μL rxn 250