上海金畔代理Lumiprobe品牌活性荧光染料产品

上海金畔代理Lumiprobe品牌活性荧光染料产品
Lumiprobe Corporation是美国一家高品质生物技术公司,专业提供分子生物学研究用的活性荧光染料。从2006年开始,公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有:活性染料(Reactive Dye)和SYBR Green I 染料,用于寡核苷酸合成的亚磷酰胺,点击化学用染料和其它试剂。 产品主要应用:点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。其中,氨基类染料是包含自由氨基的活性染料,染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如:氨基与EDC-活化的羧基结合。
    上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商,Lumiprobe上海金畔生物官网:http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品,产品货期10个工作日左右,满足您的各种科研实验需求。Lumiprobe产品包括:羧酸类染料、羰基类染料,标记分子氨基活性染料,标记分子巯基活性染料,动物活体成像用染料,蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规,质量保证,价格优惠。
炔烃 Dye alkynes
  • 1-Ethynyl pyrene
  • 3-Ethynyl perylene
  • BDP FL alkyne
  • FAM alkyne, 5-isomer
  • FAM alkyne, 6-isomer
  • TAMRA alkyne, 5-isomer
  • BDP TMR alkyne
  • Cyanine3 alkyne
  • Cyanine5 alkyne
  • Cyanine5.5 alkyne
  • Cyanine7 alkyne
  • Cyanine7.5 alkyne
  • Sulfo-Cyanine3 alkyne
  • Sulfo-Cyanine5 alkyne
叠氮化物 Dye azides
  • Pyrene azide 1/ Pyrene azide 2
  • PEP azide
  • Perylene azide
  • BDP FL azide
  • Coumarin 343 azide
  • FAM azide, 5-isomer
  • FAM azide, 6-isomer
  • TAMRA azide, 5- isomer
  • BDP TMR azide
  • Cyanine3 azide
  • Cyanine3.5 azide
  • Cyanine5 azide
  • Cyanine5.5 azide
  • Cyanine7 azide
  • Cyanine7.5 azide
  • Sulfo-Cyanine3 azide
  • Sulfo-Cyanine5 azide
  • Sulfo-Cyanine7 azide
N-羟基琥珀酰亚胺脂Dye NHS esters
  • Pyrenebutyric acid NHS ester
  • ROX NHS ester, pure 6- isomer
  • BDP FL NHS ester
  • FAM NHS ester
  • Cyanine3 NHS ester
  • Cyanine3.5 NHS ester
  • Cyanine5 NHS ester
  • Cyanine5.5 NHS ester
  • Cyanine7 NHS ester
  • Cyanine7.5 NHS ester
  • Sulfo-Cyanine3 NHS ester
  • Sulfo-Cyanine5 NHS ester
  • sulfo-Cyanine5 bis-NHS ester
  • Sulfo-Cyanine7 NHS ester
马来酰亚胺 Dye maleimides
  • Pyrene maleimide
  • BDP FL maleimide
  • Cyanine3 maleimide
  • Cyanine5 maleimide
  • Cyanine5.5 maleimide
  • Cyanine7 maleimide
  • Cyanine7.5 maleimide
  • Sulfo-Cyanine3 maleimide
  • Sulfo-Cyanine5 maleimide
伯胺 Primary amine
  • BDP FL amine
  • Cyanine3 amine
  • Cyanine5 amine
  • Cyanine5.5 amine
  • Cyanine7 amine
  • Cyanine7.5 amine
  • Sulfo-Cyanine5 amine
  • Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
  • Pyrene hydrazide
  • BDP FL hydrazide
  • Cyanine3 hydrazide
  • Cyanine5 Boc-hydrazide
  • Cyanine5 hydrazide
  • Cyanine5.5 hydrazide
  • Cyanine7 hydrazide
  • Cyanine7.5 hydrazide
马来酰亚胺 Dye maleimides
  • BDP FL maleimide
  • Cyanine3 maleimide
  • Cyanine5 maleimide
  • Cyanine5.5 maleimide
  • Cyanine7 maleimide
  • Cyanine7.5 maleimide
  • Sulfo-Cyanine3 maleimide
  • Sulfo-Cyanine5 maleimide
羧酸 Carboxylic acids
  • Pyrenebutyric acid
  • BDP FL carboxylic acid
  • Cyanine3 carboxylic acid
  • Cyanine3.5 carboxylic acid
  • Cyanine5 carboxylic acid
  • Cyanine5.5 carboxylic acid
  • Cyanine7 carboxylic acid
  • Cyanine7.5 carboxylic acid
  • Sulfo-Cyanine3 carboxylic acid
  • Sulfo-Cyanine5 carboxylic acid
  • Sulfo-Cyanine7 carboxylic acid
点击化学试剂

非荧光炔烃 Non-fluorescent alkynes

  • Pentynoic acid STP ester
  • Biotin alkyne
非荧光叠氮化物 Non-fluorescent azides
  • Azidobutyric acid NHS ester
  • Biotin azide
寡核苷酸分析
亚磷酰胺 Phosphoramidites
  • FAM Phosphoramidite, 6-Isomer
  • Alkyne Amidite, hydroxyprolinol
  • Alkyne Phosphoramidite, 5′-terminal
  • DMS(O)MT aminolink C6
  • Long trebler phosphoramidite
  • Pyrene phosphoramidite dU
PCR/qPCR PCR/qPCR
  • ROX reference dye for qPCR
  • SYBR Green I Gel Staining Solution, 10000x
  • SYBR Green I for Real Time PCR, 100x
CPG固相修饰 CPG solid supports
  • Alkyne CPG modifier 500
  • TFA-Amino modifier CPG 500
三磷酸酯修饰 Modified triphosphates
  • Amino-11-ddUTP
  • Amino-11-dUTP
其它试剂
蛋白质组学试剂 Proteomic reagents
  • 3Dye™ 2D DIGE Kits
  • Cyanine2 NHS ester minimal dye
  • Cyanine3 NHS ester minimal dye
  • Cyanine5 NHS ester minimal dye
辅助试剂 Auxiliary reagents
  • Ascorbic acid
  • Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
  • DMF (dimethylformamide), labeling grade

Lumiprobe芘叠氮化物 1,Pyrene azide 1

Lumiprobe芘叠氮化物 1,Pyrene azide 1

Pyrene属于多环芳烃,拥有较强短波荧光。不像其他的荧光染料,多环芳烃类属于对微环境有较强灵敏度的荧光探针。因此,它的荧光性在极性和非极性环境中是不同的。其他的影响也可以被观察到。 当两个pyrenes靠的很近时,他们便表现出激发态。激发态形式很容易被观察到,使用荧光波谱图可以被定量估测。

Pyrene属于多环芳烃,拥有较强短波荧光。不像其他的荧光染料,多环芳烃类属于对微环境有较强灵敏度的荧光探针。因此,它的荧光性在极性和非极性环境中是不同的。其他的影响也可以被观察到。
当两个pyrenes靠的很近时,他们便表现出激发态。激发态形式很容易被观察到,使用荧光波谱图可以被定量估测。
Pyrene azide可以比较容易的应用于pyrene点击化学,对难以进行炔烃标记的分子进行标记。
它也可以将分子引入难以标记pyrene的探针。
这种叠氮染料含有亲水三甘醇链以缓解其固有的疏水性并促进在水中对生物分子的标记。
另两种点击化学用pyrene azides(23)。

Pyrene is a polyaromatic hydrocarbon with strong short-wavelength fluorescence. Unlike other fluorescent dyes, polyaromatic hydrocarbons are fluorescent probes with strong sensitivity to microenvironment. Thus, its fluorescence is different in polar, and nonpolar environments. Other effects can also be observed.

When two pyrenes are in close proximity, they form excimers. Excimer formation can be easily observed, and quantitatively estimated using fluorescent spectra.

Pyrene azide is a reagent for easy pyrene Click Chemistry labeling of any alkyne-bearing molecule. It allows to turn any molecule into pyrene-bearing probe.

This azide contains hydrophilic triethyleneglycol linker to mitigate intrinsic pyrene hydrophobicity, and facilitate attachment to biomolecules in aqueous solutions.

Another pyrene azide for Click Chemistry labeling is also available: Pyrene azide 2.

订购货号 产品名称及规格
A1530 Pyrene azide 1, 1 mg
B1530 Pyrene azide 1, 5 mg
C1530 Pyrene azide 1, 10 mg
D1530 Pyrene azide 1, 25 mg
E1530 Pyrene azide 1, 50 mg

General properties

Appearance: yellowish solid
Molecular weight: 402.45
Molecular formula: C23H22N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR聽1H (95%)
Storage conditions: Storage: 24 months after receival at -20掳C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 343; 326; 313; 276; 265; 242; 234
Emission maximum, nm: 377; 397

Lumiprobe Perylene azide荧光染料

Lumiprobe Perylene azide荧光染料

Perylene是一种高亮度的且对公极其稳定的多环芳烃(PAH)类荧光染料,其量子产率接近理想化。
因其很短的荧光期,这种探针没有激发态。
因其较低的溶解度和很少量的活性衍生物,Perylene标记生物分子通常比较困难且不太容易实现。然而,通过点击化学使用这种perylene azide来进行标记就变得和其他染料一样非常容易。使用这种多环芳烃染料标记多肽、DNA、蛋白等从未如此轻松。
为了提高其亲水性和标记成功率,这种PAH azide包含亲水三甘醇链。

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with quantum yield approaching quantitative.

Due to low life time of fluorescence, this probe does not form excimers.

Perylene labeling of biomolecules is used to be difficult and tedious because of low solubility of perylene, and low availability of activated derivatives. However, labeling via Click Chemistry with this perylene azide is nearly as easy, as with other dyes. Labeling peptide, DNA, protein with this polyaromatic hydrocarbon has never been so simple yet.

To increase hydrophilicity, and facilitate labeling, this PAH azide contains hydrophilic triethyleneglycol linker.

订购货号 产品名称及规格
A5530 Perylene azide, 1 mg
B5530 Perylene azide, 5 mg
C5530 Perylene azide, 10 mg
D5530 Perylene azide, 25 mg
E5530 Perylene azide, 50 mg

General properties

Appearance: orange-yellow solid
Molecular weight: 452.50
Molecular formula: C27H24N4O3
Solubility: good in dichloromethane and chloroform, moderate in DMSO, DMF, and acetonitrile
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

细胞膜染料–DiD,DiO,DiI,DiR 和 DiS

细胞膜染料–DiD,DiO,DiI,DiR 和 DiS

产品描述

DiD(CAS:127274-91-3),DiO(CAS:34215-57-1),DiI(CAS:41085-99-8),DiR(CAS:100068-60-8)和DiS染料是一族亲脂性的荧光染料,可以用来染细胞膜和其它脂溶性生物结构。当与细胞膜结合后其荧光强度大大增强,这类染料有着很高的淬灭常数和激发态寿命。一旦对细胞染色,这类染料在整个细胞膜上扩散,佳浓度时可以使整个细胞膜染色。它们的荧光颜色区分明显:DiI(橙色荧光),DiO(绿色荧光),DiD(红色荧光)和 DiR (深红色荧光)这使得他们可以用来对活细胞进行多色成像和流式分析。DiI和DiO可以分别用标准的 FITC和TRITC的滤光片。DiD可以用 633 nm He–Ne 激光器激发,有着比DiI更长的激发波长和发射波长,在细胞和组织染色中更有价值。 DiR的红外荧光可以穿透细胞和组织,在活体成像中用来示踪。

**DiR碘化物的发射波长肉眼不可见,所以需要配备CCD镜头或其他的近红外检测仪器。

Di系列染料想必大家一定不陌生,我们做细胞膜染色的时候经常会用到,今天给大家介绍下常见的4类Di染料:DiI, DiO, DiD 和 DiR,Di系列染料是一类环境敏感型亲脂性膜染料,这类探针在进入细胞膜之前荧光非常弱,当它们与膜结合或者与亲脂性生物分子(例如蛋白质)结合时,其荧光强度显著增强。一旦应用于细胞中,这类染料会在细胞内质膜中逐步扩散,在其最佳浓度条件下,可以扩散逐渐使整个细胞的细胞膜被染色。

 

DiI, DiO, DiD 和 DiR被激发后可以发出不同颜色的荧光,DiO和磷酯双层膜结合后的激发光谱和发射光谱参考下图。

 

DiO、DiI、DiD、DiR的光谱图

DiO、DiI、DiD、DiR的光谱图

 

DiO, DiI, DiD 和 DiR几种染料的异同:

 

其中I、D、R分别代表了一个,两个,三个双键,它们是决定波长长度的关键,DiO, DiI, DiD 和 DiR这几款染料的结构式如下:

DiR碘化物结构式 Dil高氯酸盐结构式
DiO高氯酸盐结构式 Dil高氯酸盐结构式
DiD高氯酸盐结构式 DiR碘化物结构式
DiD高氯酸盐结构式 DiR碘化物结构式

 

DiO, DiI, DiD 和 DiR几种染料的异同:

名称 荧光颜色 应用 货号
DiO(DiOC18(3)) 绿色荧光 • DiO被广泛用于正向或逆向的,活的或固定的神经等细胞或组织的示踪剂或长期示踪剂,通常不会明显影响细胞的生存力,对于细胞膜染色的荧光强度通常要低于DiI,有时对于某些经过固定的组织的染色效果欠佳。• 还可以用于检测细胞的融合和粘附,检测发育或移植过程中细胞迁移。 22066
DiI (DiIC18(3)) 橙色荧光 • DiI被广泛用于正向或逆向的,活的或固定的神经等细胞或组织的示踪剂或长期示踪剂,通常不会影响细胞的生存力。• DiI除了最简单的细胞膜荧光标记外,还可以用于检测细胞的融合和粘附,检测发育或移植过程中细胞迁移,通过FRAP检测脂在细胞膜上的扩散,检测细胞毒性和标记脂蛋白等。 22102
DiD (DiIC18(5)) 红色荧光 • DID染料的染色效率高,均一,不易猝灭,无明显细胞毒性,且受自身荧光背景干扰小。• 目前DID已成功应用于多种细胞的体内示踪研究,如Treg细胞、间充质干细胞、肿瘤细 胞、造血干祖细胞等。 22033
DiR (DiIC18(7)) 深红色荧光 • DiR的红外荧光可以穿透细胞和组织,在活体成像中用来示踪。常用于标记细胞质膜。两个18-碳链插入到细胞膜,从而特定的、稳定的细胞染色,几乎不会发生细胞间的染料转移。可以用乙醇配置其储存液。• DiR 染色后可进行多聚甲醛(不可使用甲醇等其他试剂)的固定,但不建议在染色后进行透化的过程。 22070

 产品优点:

1. 应用广泛:主要作用是标记细胞膜,用于活的或固定的神经元等细胞或组织的长期示踪剂,同时还可以标记其他疏水结构,例如外泌体

2. 不会影响细胞的生存力

3. 体外培养的条件下可以存活很长时间

4. 在经过固定的神经元细胞膜上的迁移速率为 0.2-0.6mm/day

5. 在活的神经元细胞膜上的的迁移速率为 6mm/day

关于染料的使用技巧:

1.DiI 可以溶解于无水乙醇、DMSO 和 DMF,溶解度约为 1-2.5mg/ml,发现较难溶解时可以适当用超声处理以促进溶解。

2.用于细胞膜荧光标记时,DiI 的常用浓度为 1-25μM,最常用的浓度为 5-10 μM。

3. DiI 可以直接染色活的细胞或组织,染色时间通常为 5-20 分钟。

4. 染固定的细胞时,通常使用 4%多聚甲醛进行固定,使用其它不适当的固定液会导致荧光背景较高。

Di家族荧光染料选择小tips:

DiI, DiO, DiD 和 DiR均可染色活细胞或固定细胞及组织(请您根据您的需求选择相应的探针),DiI比DiO荧光更亮;DiD,DiR(D12731)波长更长,更适合组织染色;

DiR应用实列赏析:

DiR应用实列 DiR应用实列
DiR应用实列 DiR应用实列
DiR应用实列 DiR应用实列

源文献:Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model Journal: Scientific Reports (2017)

DiD,DiO,DiI,DiR荧光染料之间的区别

DiD染料是一族亲脂性的荧光染料,可以用来染细胞膜和其它脂溶性生物结构。当与细胞膜结合后其荧光强度大大增强,这类染料有很高的淬灭常数和激发态寿命。一旦对细胞染色,染料在整个细胞膜上扩散,zui佳浓度时可以使整个细胞膜染色。

绿色荧光、亲脂性羰花青DiOC18(3)被广泛用作亲脂示踪剂。它在水里是弱荧光的,但当嵌入膜时,它的荧光和光稳定性都很好。

DiI是一种亲脂性膜染料,进入细胞膜后可以侧向扩散逐渐使整个细胞的细胞膜被染色。DiI在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiI被广泛用于正向或逆向的,活的或固定的神经等细胞或组织的示踪剂或长期示踪剂,通常不会影响细胞的生存力。

DIR是一个亲脂性、近红外荧光花青染料。这个染料常用于标记细胞质膜。两个18-碳链插入到细胞膜,从而特定的、稳定的细胞染色,几乎不会发生细胞间的染料转移。可以用乙醇配置其储存液。细胞染色通常使用的浓度时1-10uM,孵育10-20分钟的。

它们的荧光颜色区分明显:DiI(橙色荧光),DiO(绿色荧光),DiD(红色荧光)和 DiR (深红色荧光)这使得他们可以用来对活细胞进行多色成像和流式分析。DiI和DiO可以分别用标准的 FITC和TRITC的滤光片。DiD可以用 633 nm He–Ne 激光器激发,有着比DiI更长的激发波长和发射波长,在细胞和组织染色中更有价值。 DiR的红外荧光可以穿透细胞和组织,在活体成像中用来示踪。

使用方法
1. DiD,DiO,DiI,DiR和DiS细胞膜染色液制备
(1)配置DMSO或EtOH 储存液:储存液用 DMSO或EtOH配置浓度1~5 mM。
注意:未使用的储存液保存在-20 oC,避免反复冻融。
(2)工作液制备:用合适的缓冲液(如:无血清培养基,HBSS或PBS)稀释储存液,配制浓度为1~5 µM的工作液。
注意:工作液的最终浓度是根据不同细胞和实验的经验来配制。可以从推荐浓度的十倍以上寻找佳条件。
2. 悬浮细胞染色
(1)悬浮细胞密度为 1 × 106/mL加入到工作液中。
(2)在37 ℃培养细胞 2~20分钟,不同的细胞佳培养时间不同。
(3)染色细胞试管在1000~1500转离心5分钟。
(4)倾倒上清液,再次缓慢加入预温37℃的培养液。
(5)重复(3),(4)步骤两次以上。
3. 粘壁细胞的染色
(1)使粘壁的细胞在无菌实验室培养。
(2)从培养基中移走盖玻片,吸走过量培养液,将盖玻片放在潮湿的环境中。
(3)在盖玻片的一角加入100μL的染料工作液,轻轻晃动使染料均匀覆盖所有细胞。
(4)在37 ℃培养细胞 2~20分钟,不同的细胞佳培养时间不同。
(5)吸干染料工作液,用培养液洗盖玻片2~3次,每次用预温的培养基覆盖所有细胞,培养5~10分钟,然后吸干培养基。
4. 显微镜检测
(1)DiD,DiO,DiI,DiR和DiS滤光器的选择参见表1。
(2)多色染料的同时检测,滤光器按照以下设定:
a) DiI和DiO=Omega XF52,Chroma 51004;
b) DiI和DiD=Omega XF92,Chroma 51007;
c) DiI,DiO和DiD=Omega XF93,Chroma 61005
5. 流式细胞仪的检测
DiD,DiO,DiI,DiR和DiS 染色的细胞可以分别用经典的FL1,FL2,FL3和FL4流式细胞仪检测。

储存条件:DiR碘化物-20℃干燥避光保存,有效期一年。

细胞膜荧光探针DiR,碘化物

DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]

别名:DiR碘化物  
分子量:1013.39
溶剂:DMSO

保存:-20°C干燥避光保存
有效期:12个月

产品描述 细胞膜荧光探针DiR,碘化物产品的光谱学相关参数为Ex=748nm / Em=780nm;其对应分子量大小为1013.39MW.
产品形式 提供的细胞膜荧光探针DiR,碘化物产品为固体形式。我们建议将其溶解到二甲基亚砜(DMSO)溶液中使用或保存。
保存建议 对于 细胞膜荧光探针DiR,碘化物 产品,我们推荐使用常温进行运输.在您收到货物后,我们建议在低于-15℃温度下冷冻保存,要求储存环境干燥并避免光照.产品有效期为12个月.
目录号 产品名称 规格 分子量 Ex/Em
DiD
22033 DiD labeling solution 10ml 959.91 644/663nm
22034 DiD Perchlorate *UltraPure Grade* 25mg 959.91 644/663nm
DiO
22038 DiOC2(3)iodide 100mg 881.7 484/501nm
22039 DiOC3(3)iodide 100mg 488.32 482/497nm
22040 DiOC7(3)iodide 100mg 600.57 482/504nm
22042 DiOC16(3)perchlorate 25mg 825.6 484/501nm
22045 DiOC5(3)iodide 100mg 544.47 482/504nm
22046 DiOC6(3)iodide 100mg 572.52 482/504nm
22066 DiO perchlorate 100mg 881.7 482/501nm
DiI
22035 DiIC12(3)perchlorate 100mg 765.55 549/565nm
22044 DiIC16(3)perchlorate 100mg 877.76 549/565nm
22052 DiIC18(3)-DS 50mg 993.53 555/570nm
22054 DiIC18(5)-DS 50mg 1019.57 650/670nm
22056 DiIC1(5)iodide 100mg 510.45 638/658nm
22101 DiI iodide 100mg 961.32 549/565nm
22102 DiI perchlorate 100mg 933.87 549/565nm
22103 DiI triflate 100mg 983.48 549/565nm
DiR
22070 DiR iodide 10mg 1013.39 748/780nm
DiS
22073 DiSC2(3) 25mg 492.44 560/571nm
22076 DiSC3(5) 100mg 546.53 660/675nm

Lumiprobe 抗体标记试剂盒说明书Antibody labeling kits

Lumiprobe 抗体标记试剂盒说明书Antibody labeling kits

The kit is used for the preparation of labeled antibodies having 2.5-3 labels per antibody. The kit contains all reagents and materials for ten labeling reactions, each for 100 ug of the antibody. The dye is an NHS ester, taken in controlled excess. It reacts with the amine groups of lysines the antibody at mild alkaline pH. The purification of the antibody is achieved by gel filtration using spin columns. These kits contain sulfonated, water soluble dyes, that are most suitable for the labeling of sensitive proteins, including antibodies.

Kit components

Kit component Count
1321-10rxn
10 reactions
3321-10rxn
10 reactions
7321-10rxn
10 reactions
5321-10rxn
10 reactions
6321-10rxn
10 reactions
N1320, Sulfo-Cyanine3 NHS ester, 1 rxn 10
N3320, Sulfo-Cyanine5 NHS ester, 1 rxn 10
N7320, Sulfo-Cyanine5.5 NHS ester, 1 rxn 10
N5320, Sulfo-Cyanine7 NHS ester, 1 rxn 10
N6320, Sulfo-Cyanine7.5 NHS ester, 1 rxn 10
Desalting spin column, PBS 10 10 10 10 10
Desalting receptacle vial, 1.5 mL 10 10 10 10 10
Desalting waste vial, 2 mL 10 10 10 10 10
PBS tablet, for 100 mL of buffer 1 1 1 1 1
15050, DMSO, labeling grade, 1 mL 1 1 1 1 1
1584-05mL, Sodium azide solution, 3%, 0.5 mL 1 1 1 1 1
1689-15mL, Sodium bicarbonate, 126 mg 1 1 1 1 1

Store at temperature between +3 °C and +20 °C. Do not freeze!

Shelf life 12 months

Protocol

1. Antibody preparation.

Optimal conditions for the labeling reaction are the following: antibody at a concentration of 1 mg/mL dissolved in 0.1 M sodium hydrocarbonate solution. Sodium azide is compatible with the labeling. If concentration of antibody is below 1 mg/mL, it should be concentrated. Antibody preparation should be free of aminoacids, proteins like BSA and buffer components that bring pH to unfavourable (2-7.5 or 9-12) ranges.

2. Setting up the reaction.

2.1. Add a solution of antibody in sodium hydrocarbonate (100 ug in 100 uL*) to the fluorophore, vortex, and incubate for 30 min at room temperature.

* if a smaller amount of antibody is to be labeled, dissolve lyophilized dye in 10 uL of anhydrous DMSO, and take 1 uL of the solution per 10 ug of antibody. The solution of dye in DMSO should be consumed immediately.

3. Purification of the labeled antibody.

3.1. Prepare the column. The column should be at room temperature prior to use. The gel should be resuspended using vortex. Put the column into a waste receptacle (without cap), and centrifuge for 2 min at 1,000 g (speed must be carefully controlled – for standard 6 cm rotor, 1,000 g = 3800 rpm). The small bulge of the column should be oriented outwards.

3.2. Apply 400 uL of 1x PBS buffer, centrifuge for 2 min at 1,000 g.

3.3. Remove the column from waste receptacle. Put it into collection tube (with cap). Apply 100 uL of the reaction mixture onto the column, incubate for 1 min, and elute the antibody by centrifugation for 2 min at 1,000 g. Add 1/100 volume of 100x sodium azide. Antibodies can be aliquoted to improve their shelf life. Current aliquot can be stored at +4°С the rest at -20°С.

4. Measurement of dye to antibody ratio.

To calculate dye to antibody ratio, measure absorption spectrum of the conjugate, or absorption at 280 nm (AAB), and at dye absorption maximum (ADye). A typical absorption spectrum of a dye labeled antibody is shown below. Depending on the dye, wavelength of the maximum may vary.

Labeled antibody absorption spectrum

An absorption spectrum of a labeled antibody contains dye peak (with longer wavelength), and antibody peak (at around 280 nm). Dye to antibody ratio is calculated using the following formula:

Calculation of dye to antibody ratio

with Dye/AB – average number of fluorophores per antibody molecule, Adye – optical density of the sample at dye absorption maximum, AAB – sample optical density at 280 nm, εAB – molar extinction coefficient of antibody at 280 nm (210,000 for IgG), εDye – molar extinction coefficient of dye at absorption maximum (see table below), CF280 – correction factor for dye (see table below).

Dye λmax, nm ε CF280
sulfo-Cyanine3 548 162,000 0.06
sulfo-Cyanine5 646 271,000 0.04
sulfo-Cyanine5.5 673 195,000 0.11
sulfo-Cyanine7 750 240,600 0.04
sulfo-Cyanine7.5 778 222,000 0.09

Example of calculation

After labeling of IgG antibody with sulfo-Cyanine5 and purification, a solution has been obtained with absorption spectrum above. Determine dye to protein ratio.

Using absorption spectrum, the following values can be found: ADye = 0.184 at dye absorption maximum (646 nm, see the table), AAB = 0.066 (at 280 nm), εAB = 210000 for IgG; εDye = 271,000, and CF280 = 0.04.

Dye/AB – is 2.43 fluorophore molecules per antibody.

Interpretation of results, and antibody storage

In most cases, optimal dye to antibody ratio is 2-3 dye molecules per antibody. Increase of dye loading above this value does not improve fluorescent signal because of the concentrational quenching of the fluorescence. When too few fluorophores are attached during the reaction, decrease antibody loading per reaction. Use of expired kit can also lead to this result.

We recommend to test binding of labeled antibodies with their antigens. The labeled antibodies can be stored at -20°С. An aliquot that is currently in use, should be stored at +4°С to avoid freeze-thaw cycles. The stability of the conjugate is determined by antibody itself rather than fluorophore or the chemical bond. Labeled antibodies should not be exposed to direct sunlight for extended time, but tolerate ambient lighting well.

Related kits

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.

Show pricing

sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.

Show pricing

sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.

Show pricing

sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.

Show pricing

sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.

Show pricing

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

Lumiprobe 蛋白质标记试剂盒 Protein labeling kits

上海金畔生物代理Lumiprobe品牌产品,欢迎新老客户访问Lumiprobe官网或者咨询我们获取更多相关产品信息。

Lumiprobe protein labeling kits contain pre-aliquoted reactive dye, all necessary auxiliary components and buffers, and desalting spin columns to achieve efficient, reproducible labeling of antibodies, and other proteins.

The labeled protein is purified by means of gel filtration on gel filled spin columns that are enclosed. This protocol ensures complete removal of the unbound dye, and excellent recovery of the labeled product. The protein is eluted in PBS buffer, ready for the use in most downstream applications.

The protocol is quick, effortless, and easy to follow.

Lumiprobe 探针蛋白标记试剂盒包含预先引用的活性染料、所有必需的辅助成分和缓冲器,以及脱盐自旋柱,以实现抗体和其他蛋白质的高效、可复制的标记。

标记蛋白是通过凝胶过滤纯化的凝胶填充自旋柱是封闭的。该协议确保完全去除未结合染料,并出色地恢复标记产品。该蛋白在PBS缓冲液中被洗脱,可以在大多数下游应用中使用。

该试剂盒具有快速,毫不费力,易于遵循等优点。

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.


sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.


sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.


sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.


sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.

Lumiprobe 23420 BDP TR NHS ester

Lumiprobe 23420 BDP TR NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

BDP TR is a bright and photostable borondipyrromethene dye, which is especially suitable for microscopy applications. The dye has a long excited state lifetime, and is therefore is useful for fluorescence polarization assays. Its large two-photon cross section makes this dye useful for two-photon spectroscopy.

This NHS ester is an amine-reactive form of the dye.

Lumiprobe 23420 BDP TR NHS ester

Cat. # Quantity Lead time
13420 1 mg in stock
23420 5 mg in stock
43420 25 mg in stock
53420 50 mg in stock
63420 100 mg in stock

BDP TR NHS ester structure

Absorption and emission spectra of BODIPY TR

Absorption and emission spectra of BODIPY TR

General properties

Appearance: dark blue-black crystals
Mass spec M+ increment: 406.1
Molecular weight: 521.30
Molecular formula: C25H18BF2N3O5S
IUPAC name: Methyl {p-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]phenoxy}acetate
Quality control: HPLC-MS (95%), UV-Vis
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 589
ε, L⋅mol−1⋅cm−1: 69000
Emission maximum, nm: 616
Fluorescence quantum yield: 0.9
CF260: 0.15
CF280: 0.19

Lumiprobe Bodipy(BDP)活性染料产品列表

BDP 558/568 NHS ester

BDP 581/591 NHS ester

BDP 630/650 X NHS ester

BDP FL NHS ester

BDP R6G NHS ester

BDP TMR NHS ester

BDP TR NHS ester

BDP 558/568 azide

BDP 581/591 azide

BDP 630/650 azide

BDP FL azide

BDP R6G azide

BDP TMR azide

BDP TR azide

BDP 558/568 alkyne

BDP 581/591 alkyne

BDP 630/650 alkyne

BDP FL alkyne

BDP R6G alkyne

BDP TMR alkyne

BDP TR alkyne

BDP 581/591 maleimide

BDP 630/650 maleimide

BDP FL maleimide

BDP R6G maleimide

BDP TMR maleimide

BDP TR maleimide

BDP 581/591 hydrazide

BDP 630/650 hydrazide

BDP FL hydrazide

BDP TR hydrazide

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 581/591 tetrazine

BDP 630/650 tetrazine

BDP FL tetrazine

BDP TR tetrazine

BDP 581/591 DBCO

BDP FL DBCO

BDP R6G DBCO

BDP TR methyl ester, 5 mM in DMSO

Lumiprobe 26420 BDP 581/591 NHS ester

Lumiprobe 26420 BDP 581/591 NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe 26420 BDP 581/591 NHS ester

BDP 581/591 is a borondipyrromethene dye that has relatively long fluorescence lifetime and two photon excitation cross section. It is useful for fluorescence polarization assays. Due to the presence of a diene system, it can react with reactive oxygen species (ROS) with a change of fluorescence.

This is the NHS ester derivative for the conjugation with primary and secondary amine groups of proteins, peptides, and other molecules.

Cat. # Quantity Lead time
16420 1 mg in stock
26420 5 mg in stock
46420 25 mg in stock
56420 50 mg in stock
66420 100 mg in stock

 

BDP 581/591 NHS ester

Structure of BDP 581/591 NHS ester

Absorption and emission spectra of BDP 581/591

Absorption and emission spectra of BDP 581/591

General properties

Appearance: dark colored solid
Mass spec M+ increment: 374.1
Molecular weight: 489.28
Molecular formula: C26H22N3BF2O4
Solubility: good in DMF, DMSO, DCM
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

Spectral properties

Excitation maximum, nm: 585
Emission maximum, nm: 594
CF260: 0.06
CF280: 0.04

Product citations

  1. Tabe, H.; Sukenobe, K.; Kondo, T.; Sakurai, A.; Maruo, M.; Shimauchi, A.; Hirano, M.; Uno, S.-N.; Kamiya, M.; Urano, Y.; Matsushita, M.; Fujiyoshi, S. Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix. The Journal of Physical Chemistry B, 2018, 122(27), 6906–6911. doi: 10.1021/acs.jpcb.8b03977

 

Lumiprobe Bodipy(BDP)活性染料产品列表

Lumiprobe Bodipy(BDP)活性染料产品列表

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

欢迎访问Lumiprobe官网或者咨询我们获取更多产品信息。

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe Bodipy(BDP)活性染料产品列表

BDP 558/568 NHS ester

BDP 581/591 NHS ester

BDP 630/650 X NHS ester

BDP FL NHS ester

BDP R6G NHS ester

BDP TMR NHS ester

BDP TR NHS ester

BDP 558/568 azide

BDP 581/591 azide

BDP 630/650 azide

BDP FL azide

BDP R6G azide

BDP TMR azide

BDP TR azide

BDP 558/568 alkyne

BDP 581/591 alkyne

BDP 630/650 alkyne

BDP FL alkyne

BDP R6G alkyne

BDP TMR alkyne

BDP TR alkyne

BDP 581/591 maleimide

BDP 630/650 maleimide

BDP FL maleimide

BDP R6G maleimide

BDP TMR maleimide

BDP TR maleimide

BDP 581/591 hydrazide

BDP 630/650 hydrazide

BDP FL hydrazide

BDP TR hydrazide

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 558/568 carboxylic acid

BDP 581/591 carboxylic acid

BDP 630/650 carboxylic acid

BDP FL carboxylic acid

BDP R6G carboxylic acid

BDP TMR carboxylic acid

BDP TR carboxylic acid

BDP 581/591 tetrazine

BDP 630/650 tetrazine

BDP FL tetrazine

BDP TR tetrazine

BDP 581/591 DBCO

BDP FL DBCO

BDP R6G DBCO

BDP TR methyl ester, 5 mM in DMSO

Lumiprobe BDP FL NHS ester

Lumiprobe BDP FL NHS ester

品牌:Lumiprobe

官方代理商:上海金畔生物科技有限公司(有授权证书)

货期:仅需要5-7工作日,不需要像其他家攒单导致货期3-4周。

Lumiprobe BDP FL NHS ester

Cat. # Quantity Lead time
11420 1 mg in stock
21420 5 mg in stock
41420 25 mg in stock
51420 50 mg in stock
61420 100 mg in stock

BDP FL NHS ester is an advanced dye for 488 nm channel, a replacement for fluorescein, a molecule identical to BODIPY FL® NHS ester. An amino-reactive dye for the labeling of proteins and peptides.

While the absorbance and emission spectra of this molecule stay within FAM excitation and emission channels, this dye provides much better photostability, and outstanding brightness. The fluorescence spectrum of BODIPY-FL is narrower than that of FAM. This provides a better brightness for monochromator based instruments, when emission wavelength can be tuned to dye maximum.

The dye is neutral, possesses low molecular weight, and retains high quantum yield in conjugates.

The dye is a good replacement for fluorescein (FAM), BODIPY-FL, Alexa Fluor 488, DyLight 488, Cy2, and other 488 nm dyes.

BDP FL NHS ester

Photo of BDP FL solution

Structure of BODIPY FL NHS ester

Absorption and emission spectra of BDP FL

Absorption and emission spectra of BDP FL

General properties

Appearance: orange solid
Mass spec M+ increment: 274.1
Molecular weight: 389.16
CAS number: 146616-66-2
Molecular formula: C18H18BF2N3O4
Solubility: Good in organic solvents (DMF, DMSO, dichlromethane), limited in water
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm: 503
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 509
Fluorescence quantum yield: 0.97
CF260: 0.015
CF280: 0.027

Product citations

  1. Poreba, M.; Rut, W.; Vizovisek, M.; Groborz, K.; Kasperkiewicz, P.; Finlay, D.; Vuori, K.; Turk, D.; Turk, B.; Salvesen, G.; Drag, M.Selective imaging of human cathepsin L in breast cancer by fluorescent activity-based probes. Chemical Science, 2018, 9(8), 2113–2129. doi: 10.1039/C7SC04303A
  2. Grube, L.; Dellen, R.; Kruse, F.; Schwender, H.; Stuehler, K.; Poschmann, G. Mining the secretome of C2C12 muscle cells: Data dependent experimental approach to analyze protein secretion using label-free quantification and peptide based analysis. Journal of Proteome Research, 2018, 17(2), 879–890. doi: 10.1021/acs.jproteome.7b00684
  3. Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  4. Gaspar, I.; Wippich, F.; Ephrussi, A. Enzymatic production of single molecule FISH and RNA capture probes. RNA, 2017, 23(10), 1582–1591. doi: 10.1261/rna.061184.117

Bodipy(BDP)氟硼荧光活性染料发展历史

Bodipy二吡咯类荧光染料相对比于荧光素、罗丹明或者菁染料有着较高的选择性、高灵敏度、高荧光量子产率、高摩尔消光系数、良好的光学稳定性和对PH不敏感的优点被广泛应用于生物标记、荧光探针及生物成像,西安瑞禧生物科技有限公司可以提供种类丰富的Bodipy荧光染料,荧光波长可定制 从550-800之间,末端可以修饰各种活性基团。

Bodipy类荧光染料具有良好的稳定性、优良的光学性能,主要表现在以下几个方面:

1:BODIPY的光谱峰宽比较窄,在光学领域和生物学领域进行分析检测时,光谱峰宽较窄,检测灵敏度较高,并且减少了待测样品的用量。测得的结果中,荧光光谱上会出现更为明显的荧光吸收和发射的变化,这样更精确地测得了样品的含量。

2:在整个BODIPY分子中,并不存在负电荷。因而在溶液中,溶液中的离子无法与缺少电荷的BODIPY分子发生静电作用,这样非常有效的避免了测定时静电作用对测量结果的干扰,使测定过程更加稳定,测得结果更加精准。

3:BODIPY类荧光染料的光稳定性能非常优越,当它被激发到达激发态时,不会因为很长时间的激发而在分析测定过程中发生激发态的分解或其他变化,这样测得的荧光光谱更加准确可信。

4:BODIPY染料,其荧光量子产率甚至接近1.0。一般普通的染料在水溶液中其荧光量子产率会变低,有的甚至被猝灭。对于BODIPY分子来说,在水溶液中其荧光量子产率虽然有所下降,但它仍然能保持较高的荧光量子产率。

5:由BODIPY的母体结构可知,他的母体结构上的取代基一般不会出现氨基或羧基这类对酸碱敏感的活性基团。因此外界的酸碱度对其影响会大幅度减小,因此BODIPY染料不会轻易变质。

6:BODIPY类荧光染料对光的感应度很高,并且它还有高的摩尔消光系数,有些经过修饰后的BODIPY染料在水溶液中的溶解度也较高,再加上它在水中不易被猝灭这一优异的性能,使得BODIPY在光学、生物学、分析学领域有着极其广泛的应用。

西安瑞禧生物科技有限公司可以提供激发和发射波长从500nm-800nm的活性染料(包括800nm左右的近红外)并且可以修饰NHS、MAL、NH2、COOH、N3、SH等不同活性基团。

常规的Bodipy荧光染料都是激发发射波长都是在500-650nm之间的,包括国外的商家也只能提供500-650nm波长的荧光染料,瑞禧生物不仅可以提供波长在500-600nm之间的波长 还可以提供650-800nm之间的近红外波段的BDP

500-800之间的波长可以选择:
Bodipy carboxylic acid(羧基COOH)
Bodipy NHS ester(活化脂NHS)
Bodipy amine (NH2氨基)
Bodipy maleimide (MAL马来酰亚胺)
Bodipy azide (N3叠氮)
Bodipy alkyne (ALK炔烃)
Bodipy hydrazide (HZ酰基)
Bodipy Thiol (HS巯基)

产品列表:
BODIPY™ FL NHS Ester (Succinimidyl Ester)
BODIPY™ FL Sulfonated Succinimidyl Ester
BODIPY™ FL-X NHS Ester (Succinimidyl Ester)
BODIPY™ TMR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 530/550 NHS Ester (Succinimidyl Ester)
BODIPY™ R6G NHS Ester (Succinimidyl Ester)
BODIPY™ 581/591 NHS Ester (Succinimidyl Ester)
BODIPY™ 576/589 NHS Ester (Succinimidyl Ester)
BODIPY™ 650/665-X NHS Ester (Succinimidyl Ester)
BODIPY™ 493/503 NHS Ester (Succinimidyl Ester)
BODIPY™ 558/568 NHS Ester (Succinimidyl Ester)
BODIPY™ TR-X NHS Ester (Succinimidyl Ester)
BODIPY™ 630/650-X NHS Ester (Succinimidyl Ester)
BODIPY™ FL maleimide
BODIPY™ FL azide
BODIPY™ FL alkyne
BODIPY™ FL hydrazide
BODIPY™ FL amine
BODIPY™ FL Carboxylic acid
BODIPY™ TR ceramide
BODIPY™ FL STP ester
BODIPY™ FL C12-sphingomyelin
BODIPY™ R6G NHS Ester
BODIPY™ 558/568 phalloidin
BODIPY™ FL hydrazide
BODIPY™ TMR-X NHS Ester
BODIPY™ FL L-cystine
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid
BODIPY™ FL Carboxylic acid

BODIPY®493/503 NHS ester
BODIPY®493/503 Carboxylic acid
BODIPY®493/503 amine
BODIPY®493/503 maleimide
BODIPY®493/503 azide
BODIPY®493/503 alkyne

BODIPY®558/568 NHS ester
BODIPY®558/568 Carboxylic acid
BODIPY®558/568 amine
BODIPY®558/568 maleimide
BODIPY®558/568 azide
BODIPY®558/568 alkyne

BODIPY®650/665 NHS ester
BODIPY®650/665 Carboxylic acid
BODIPY®650/665 amine
BODIPY®650/665 maleimide
BODIPY®650/665 azide
BODIPY®650/665 alkyne

BODIPY FL NHS Ester
BODIPY TMR NHS Ester
BODIPY TR NHS Ester
BODIPY FL amine
BODIPY TMR amine
BODIPY TR amine

 
以下染料的激发波长是685nm 发射波长是720nm
Azo-Bodipy-685  (ABDP685)
Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 carboxylic acid
Azo-Bodipy 685 NHS ester
Sulfo-Azo-Bodipy 685 NHS ester
Azo-Bodipy 685 amine
Sulfo-Azo-Bodipy 685 amine
PEG-Azo-Bodipy 685 NHS ester
PEG- Azo-Bodipy 685 amine
Azo-Bodipy 685 alkyne
Sulfo-Azo-Bodipy 685 alkyne
Azo-Bodipy 685 azide
Sulfo-Azo-Bodipy 685 azide
Azo-Bodipy 685 maleimide
Sulfo-Azo-Bodipy 685 maleimide
Azo-Bodipy 685 pH6.5

我们还接受激发波长和发射波长在500nm-800nm之间的BDP活性荧光染料的定制产品。
上面所述染料可以连接:COOH,  NH2,  NHS,  MAL,  N3,  ALK,  hydrazide,  SH

其他波长的BDP活性染料的产品列表:
产品列表:
BDP 558/568 NHS ester
BDP 581/591 NHS ester
BDP 630/650 X NHS ester
BDP FL NHS ester
BDP R6G NHS ester
BDP TMR NHS ester
BDP TR NHS ester

BDP 581/591 azide
BDP 630/650 azide
BDP FL azide
BDP R6G azide
BDP TMR azide
BDP TR azide

BDP 558/568 alkyne
BDP 581/591 alkyne
BDP 630/650 alkyne
BDP FL alkyne
BDP R6G alkyne
BDP TMR alkyne
BDP TR alkyne

BDP 581/591 maleimide
BDP 630/650 maleimide
BDP FL maleimide
BDP R6G maleimide
BDP TMR maleimide
BDP TR maleimide

BDP 581/591 hydrazide
BDP 630/650 hydrazide
BDP FL hydrazide
BDP TR hydrazide

BDP 558/568 carboxylic acid
BDP 581/591 carboxylic acid
BDP 630/650 carboxylic acid
BDP FL carboxylic acid
BDP R6G carboxylic acid
BDP TMR carboxylic acid
BDP TR carboxylic acid

BDP 581/591 amine
BDP 630/650 amine
BDP FL amine
BDP R6G amine
BDP TMR amine
BDP TR amine

BDP 581/591 tetrazine
BDP 630/650 tetrazine
BDP FL tetrazine
BDP TR tetrazine

BODIPY母体结构最初是由德国化学家Treibs, A.和kreuzer, F. H.在1968年合成的,他们合成的BODIPY 类荧光染料 [40] 。另外,他们还合成了BODIPY染料的母体结构。在BODIPY母体结构中,Treibs, A.和kreuzer, F. H.在R1-R7中用不同基团取代得到不同的衍生产物。人们根据Treibs, A.和kreuzer, F. H.的前期探索,对BODIPY类染料做出了多种结构修饰,这大大丰富了BODIPY的种类,使其迅猛发展。