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Fluorescein Avidin DCS, Cell Sorting Grade
Description
Avidin DCS, Fluorescein labeled, is a cell sorter grade of Fluorescein Avidin D. Through the development of a new method of fluorescein labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Fluorescein Avidin DCS have an optimal F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For staining cells or tissues using fluorescent microscopy, the recommended concentration range is 5-30 µg/ml with incubation times of 15-30 minutes. |
| Solution | 10 mM HEPES, 0.5 M NaCl, pH 8.0, 0.08% sodium azide, 10 mg/ml beta-cyclodextrin |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 2.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
Documents
- Double Immunofluorescent Labeling Protocol
- Fluorescence Detection of Biotin-Labeled ISH Probes – Protocol
- Fluorescence Detection of Fluorescein-Labeled ISH Probes – Protocol
- In Situ Hybridization Detection Protocol
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
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Anti-Avidin Antibody, Biotinylated Catalog #: BA-0300-.5
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VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
Citations
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Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D.
- Excitation maximum: 495 nm
- Emission maximum: 515 nm
- Color: Green