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Peanut Agglutinin (PNA), Biotinylated
Description
Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding.
Biotinylated peanut agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | DO NOT VORTEX. For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. Use of buffers containing 0.1 mMCaCl2 and 0.01 mM MnCl2 is recommended. Inhibiting/Eluting Sugar: 200 mM galactose (S-9003). |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: 200 mM galactose (S-9003). |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2 |
| Concentration | 5 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
Galactose Catalog #: S-9003
Peanut Agglutinin (PNA), Rhodamine Catalog #: RL-1072-5
Peanut Agglutinin (PNA), CY®3 Catalog #: CL-1073-1
Peanut Agglutinin (PNA), CY®5 Catalog #: CL-1075-1
Citations
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A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: 200 mM galactose