VECTASHIELD Vibrance Antifade Mounting Media are completely new formulations of curing (hard setting) antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD Vibrance Antifade Mounting Media are new tools to help investigators see more and do more with each experiment.

VECTASHIELD Vibrance offers you:

• Superior antifade/anti-photobleaching properties across the spectrum
• Compatibility with commonly used fluorophores
• View sections one hour after mounting
• No tone or autofluorescent background, even after curing
• Room temperature storage of slides with extended archiving time
• Minimal bubble formation, even after several weeks storage
• Curing formulations with choice of counterstain (DAPI) or no counterstain
• 12 month product expiration date

Plus the same features that have made the VECTASHIELD brand successful:

• Ideal refractive index
• Easy to use
• Ready to use, requires no warming
• No sealing of coverslips required (curing formulations)

VECTASHIELD Vibrance - A Brilliant Solution for Fluorescent Signal Retention!

Publications:

• Jarrin, S. et al. 2020. Differential Role of Anterior Cingulate Cortical Glutamatergic Neurons in Pain-Related Aversion Learning and Nociceptive Behaviors in Male and Female Rats. Frontiers in Behavioral Neuroscience. doi: 10.3389/fnbeh.2020.00139
• Ixmatlahua, D. et al. 2020. Neuronal Glutamatergic Network Electrically Wired with Silent but Activatable Gap Junctions. The Journal of Neuroscience. doi: 10.1523/JNEUROSCI.2590-19.2020
• Ghinassi, B. et al. 2020. Immunohistochemical Results of Soft Tissues around a New Implant Healing-Abutment Surface: A Human Study. Journal of Clinical Medicine. doi: 10.3390/jcm9041009
• Mendoza-Torreblanca, J.G. et al. 2019. Analysis of Differential Expression of Synaptic Vesicle Protein 2A in the Adult Rat Brain. Neuroscience. doi: 10.1016/j.neuroscience.2019.09.004.
• Leskelä, S. et al. 2019. C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner. Cells. doi: 10.3390/cells8101233
• Castillo-Martin, M. et al. 2019. Transformed Bone Marrow Cells Generate Neoplasms of Distinct Histogenesis. a Murine Model of Cancer Transplantation. Stem Cell Research. doi: /10.1016/j.scr.2019.101637
• Wong, W. et al. 2019. The exercise cytokine interleukin 15 rescues slow wound healing in aged mice. Journal of Biological Chemistry. doi: 10.1074/jbc.RA119.010740
• Sharma, N. et al. 2019. Pharmacological inhibition of Notch signaling regresses pre-established abdominal aortic aneurysm. Scientific Reports. doi: 10.1038/s41598-019-49682-0

VECTASHIELD Vibrance Antifade Properties

VECTASHIELD Vibrance has outstanding antifade properties, acoss the spectrum - even under far-red wavelengths - and they are compatible with most commercially available fluorophores.

Viewable in as Little as One Hour After Mounting - No Need to Wait 24 Hours

Competitor workflows for immunofluorescence recommend that slides be viewed 24 hours after mounting. VECTASHIELD Vibrance Mounting Media, however, allow same-day viewing. Accelerate discovery without sacrificing signal intensity or retention.

Improved retention of fluorescence with VECTASHIELD Vibrance Mounting Medium. Serial sections of human colon tissue (FFPE) stained for cytokeratin (mouse primary antibody AE1/AE3) and detected with fluorescein conjugated horse anti-mouse IgG secondary antibody (FI-2000). One hour after mounting with either VECTASHIELD Vibrance (top row) or a competitor mounting medium (bottom row), the sections were imaged at the intervals indicated. Note retention of fluorescent signal for section mounted with VECTASHIELD Vibrance Mounting Medium.

Extended Archiving of Mounted Sections at Room Temperature

Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD Vibrance Medium, however, can be stored for weeks at ambient temperature, in standard slide boxes, with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD Vibrance Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.

Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD Vibrance Medium, however, can be stored for weeks at ambient temperature, in standard slide boxes, with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD Vibrance Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.

With No Specimen Obscuring Retraction or Bubble Formation

Another problem commonly observed in immunoflourescence staining is the formation of bubbles over time, under the coverslip, or the shrinking (retraction) of the media. This phenomenon detracts from the overall quality of the sections and can obscure specific staining. The unique formulation of VECTASHIELD Vibrance Mounting Medium provides a uniform sheet of medium under the coverslip, with essentially no bubble formation or retraction—even after many weeks of storage at room temperature.

Serial sections of human colon tissue (FFPE) stained for cytokeratin (AE1/AE3, mouse primary) followed by Alexa Fluor™ 488 anti-mouse IgG secondary antibody. Sections were mounted with either VECTASHIELD Vibrance Mounting Medium (H-1700, left image) or competitor mounting medium (right image) stored at room temperature for two weeks, and then imaged. Note the absence of bubbles and background in green spectrum in left image (VECTASHIELD Vibrance Mounting Medium) compared with right image, after two weeks at room temperature.

Summary of VECTASHIELD Vibrance Advantages Over Competitor Mounting Media