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Lens Culinaris Agglutinin (LCA), DyLight® 649
Description
Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.
DyLight® 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
- Excitation maximum: 655 nm
- Emission maximum: 670 nm
- Color: Far red
Lens Culinaris Agglutinin (LCA), DyLight® 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 655 nm |
| Maximum Emission | 670 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2 |
| Concentration | 1 mg active conjugate/ml |
| Conjugate | DyLight 649 |
| Color of Fluorescence | Far Red |
| Sugar Specificity | Mannose, Glucose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
Lens Culinaris Agglutinin (LCA), Fluorescein Catalog #: FL-1041-5
α-methylmannoside Catalog #: S-9005
α-methylglucoside Catalog #: S-9006
Lens Culinaris Agglutinin (LCA), Biotinylated Catalog #: B-1045-5
Citations
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Lens culinaris agglutinin is composed of four subunits - two of about 17 kDa and two of 8 kDa.
LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside