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Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U.
Description
This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Features:
- Affinity-purified, ultrapure, high affinity antibody
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
- Recognizes both heavy and light chains (H+L)
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
- Can be used for tissue and cell staining, ELISAs, and blots
- Included in the VECTASTAIN® ABC kits.
- Supplied in a convenient ready-to-use prediluted, stabilized solution
- This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit
Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.
Specifications
| Unit Size | 50 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | Ready-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl. |
| Target Species | Universal (Mouse/Rabbit) |
| Conjugate | Biotinylated |
| Host Species | Horse |
| Format | Ready-to-Use |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
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Citations
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This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.