Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein
Description
The Vector M.O.M. Fluorescein Kit is specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. It contains a proprietary Mouse IgG Blocking Reagent, a M.O.M. Biotinylated Anti-Mouse Ig Reagent, and Fluorescein Avidin DCS and is based on a simple and easy to follow avidin/biotin protocol.
Key advantages of the Vector M.O.M. Immunodetection Kits:
- Clear, crisp specific staining of antigens of interest
- Based on VECTASTAIN® ABC reagent protocols
- Procedures are simple and easy to follow
- No tedious calculations or antibody prebinding steps required
Kit Contents:
- 6 ml of M.O.M. Protein Concentrate
- 1 ml M.O.M. Mouse Ig Blocking Reagent
- 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
- 0.4 ml Fluorescein Avidin DCS
The Vector® M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunofluorescence |
| Technology | ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Target Species | Mouse |
| Format | Concentrate |
| Blocking Action | Endogenous Mouse Ig |
Documents
- M.O.M. (Mouse on Mouse) Troubleshooting Guide
- User Guide
- Protocol for Multiple Immunofluorescent Staining Using Mouse Antibodies
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
For my IHC application, I intend to apply a mouse IgG primary antibody on rat tissue sections. Should I use the Vector Mouse on Mouse detection kit?
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
M.O.M.® (Mouse on Mouse) Blocking Reagent Catalog #: MKB-2213-1
M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent Catalog #: MKB-2225-.1
Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1
VECTASHIELD® Antifade Mounting Medium Catalog #: H-1000-10
VECTASHIELD® Antifade Mounting Medium with DAPI Catalog #: H-1200-10
VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Catalog #: H-1600-10
VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI) Catalog #: H-1300-10
Citations
Powered by Bioz See more details on BiozTechnical Information
The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector M.O.M. Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.
The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.
The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, Discovery Through Color: A Guide to Multiple Antigen Labeling , available on our website.
Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The Vector M.O.M. ImmPRESS® Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS™ Peroxidase Polymer reagent.