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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Vector® VIP Substrate Kit, Peroxidase (HRP)
Description
The Vector VIP HRP substrate produces an intense, violet (purple) reaction product, and can be used as an alternative to DAB or as a second color for multiple antigen labeling.
Features:
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Permanent mounting
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 300 ml of working solution
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Purple |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Multiple Labeling |
| Mounting | Non-Aqueous (Permanent) |
Kit Components
Kit Contents:
- 7 ml Vector VIP Reagent 1
- 5 ml Vector VIP Reagent 2
- 5 ml Vector VIP Reagent 3
- 7.2 ml Vector VIP Reagent 4
Documents
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase Catalog #: SP-6000-100
Methyl Green Counterstain Catalog #: H-3402-500
Hematoxylin Counterstain Catalog #: H-3401-500
Hematoxylin QS Counterstain Catalog #: H-3404-100
VectaMount® Permanent Mounting Medium Catalog #: H-5000-60
Citations
Powered by Bioz See more details on BiozTechnical Information
Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
Vector VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate. This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4° C, this kit is stable for one year.
Product review on Biocompare.com.
1. Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122
