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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 488 Anti-Rabbit (green), DyLight® 594 Anti-Mouse (red)
Description
VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.
Features:
- Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
- Affinity-purified, ultrapure, high affinity antibodies
- Secondary detection reagent optimally labeled with DyLight dyes to provide the brightest label for fluorescence microscopy
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunofluorescence |
| Target Species | Universal (Mouse/Rabbit) |
| Conjugate | DyLight 488/DyLight 594 |
| Color of Fluorescence | Kit (Green, Red) |
| Host Species | Horse |
| Format | Ready-to-Use |
Kit Components
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
- 15 ml VectaFluor Duet Reagent [DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS.
DyLight® 488:
- Excitation maximum: 493 nm
- Emission maximum: 518 nm
- Color: Green
DyLight®594:
- Excitation maximum: 592 nm
- Emission maximum: 617 nm
- Color: Red
Documents
- Safety Data Sheet
- User Guide
- Download CoA
- Datasheet
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Technical Information
Kit Contents:
- 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
- 15 ml VectaFluor Duet Reagent [DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse IgG cocktail (rabbit-green, mouse-red)] made in horse, ready-to-use in 5% NHS.
DyLight 488:
- Excitation maximum: 493 nm
- Emission maximum: 518 nm
- Color: Green
DyLight 594:
- Excitation maximum: 592 nm
- Emission maximum: 617 nm
- Color: Red
DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.
DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence. DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).