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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
M.O.M.® (Mouse on Mouse) Immunodetection Kit, Basic
Description
The M.O.M. Basic Kit is useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. The M.O.M. Kit can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems.
Key advantages of the Vector M.O.M. Immunodetection Kits:
- Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
- Clear, crisp specific staining of antigens of interest
- Based on VECTASTAIN® ABC reagent protocols
- No tedious calculations or antibody prebinding steps required
- Contains enough stock reagents to produce about 25 ml of working solution, which is generally sufficient to stain approximately 250 tissue sections.
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence |
| Technology | ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems |
| Target Species | Mouse |
| Format | Concentrate |
| Blocking Action | Endogenous Mouse Ig |
Kit Components
Kit Contents:
- 6 ml of M.O.M. Protein Concentrate
- 1 ml M.O.M. Mouse Ig Blocking Reagent
- 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
Documents
- M.O.M. (Mouse on Mouse) Troubleshooting Guide
- User Guide
- Protocol for Multiple Immunofluorescent Staining Using Mouse Antibodies
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
For my IHC application, I intend to apply a mouse IgG primary antibody on rat tissue sections. Should I use the Vector Mouse on Mouse detection kit?
Related Products
Avidin/Biotin Blocking Kit Catalog #: SP-2001
M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent Catalog #: MKB-2225-.1
M.O.M.® (Mouse on Mouse) Blocking Reagent Catalog #: MKB-2213-1
VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
Streptavidin, DyLight® 594 Catalog #: SA-5594-1
Streptavidin, DyLight® 488 Catalog #: SA-5488-1
Citations
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Four M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.
The M.O.M. ImmPRESS® Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.
An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection (for example, VECTASTAIN ABC-AP Standard Kit followed by a Vector alkaline phosphatase substrate), or choose a fluorochrome-labeled avidin or streptavidin.
Product review on Biocompare.com.