Vector Labs代理，欢迎访问Vector Labs官网或者咨询我们获取相关Vector Labs产品信息以及报价。
Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Rabbit Anti-Rat IgG Antibody, mouse adsorbed (H+L), Biotinylated
Description
Biotinylated Rabbit Anti-Rat IgG, mouse adsorbed, secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.
Features:
- Recognizes both heavy and light chains (H+L)
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
- Can be used for tissue and cell staining, ELISAs, and blots
- Supplied in solution
- The mouse adsorbed anti-rat IgG (H+L) products are designed for use with mouse tissues that may contain endogenous mouse immunoglobulins.
Specifications
| Unit Size | 0.5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | 0.5 mg active conjugate/ml |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. |
| Recommended Usage | The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species | Rat |
| Conjugate | Biotinylated |
| Host Species | Rabbit |
| Format | Concentrate |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
Horse Anti-Mouse IgG Antibody, rat adsorbed (H+L), Biotinylated Catalog #: BA-2001
Goat Anti-Rat IgG Antibody, mouse adsorbed (H+L), Biotinylated Catalog #: BA-9401-.5
ImmPRESS® HRP Goat Anti-Rat IgG, Mouse adsorbed Polymer Detection Kit, Peroxidase Catalog #: MP-7444-15
VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000
VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Streptavidin, DyLight® 488 Catalog #: SA-5488-1
Streptavidin, DyLight® 549 Catalog #: SA-5549-1
Streptavidin, DyLight® 594 Catalog #: SA-5594-1
Streptavidin, DyLight® 649 Catalog #: SA-5649-1
Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1
Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1
Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100
ImmPRESS®-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase Catalog #: MP-5444-15
Citations
Powered by Bioz See more details on BiozTechnical Information
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. With some exceptions the recommended dilution for most applications is 1:100.
The mouse adsorbed, anti-rat IgG (H+L) products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins.