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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
NEUROBIOTIN® 350 Tracer
Description
NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.
Features:
- Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
- Biotin label with a biotinidase-resistant linkage
- Fixable primary amine
- Used for visualizing neural architecture and for the identification of gap junction coupling
- Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
- Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
- Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:
- Better solubility
- More efficiently iontophoresed
- Remains in cell longer
- Non-toxic
- Can be fixed with formalin or glutaraldehyde
Specifications
| Unit Size | 2 mg |
|---|---|
| Maximum Emission | 452 nm |
| Recommended Storage | 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent. |
| Recommended Usage | NEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: •a biotin label with a biotinidase-resistant linker. •a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350. •a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde |
| Molecular Weight | 573 |
| Maximum Excitation | 346 nm |
| Neuronal Tracer – Direction of Transport | Anterograde/Retrograde |
| Detection Method | Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Related Products
NEUROBIOTIN® 488 Tracer Catalog #: SP-1125-2
NEUROBIOTIN®-PLUS Tracer Catalog #: SP-1150-5
Citations
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Similar in structure to NEUROBIOTIN 488, NEUROBIOTIN 350 is also an amine containing biotin derivative. However, it is conjugated with a bright blue fluorophore that would contrast with other green or red fluorescent markers or reagents present in the same preparation. For administration purposes NEUROBIOTIN 350 has a net positive charge and is highly soluble in a number of commonly used buffer and salt solutions.