Wako 和光纯药 高气密封储瓶 High-sealed Storage Bottle

Wako 和光纯药 高气密封储瓶 High-sealed Storage Bottle

英文名称:High-sealed Storage Bottle O-ring
中文名称:高度密封保存瓶O环
品牌:Wako
品牌中文简称:和光纯药
储存条件:室温

This product is for research use only. Do not administer it to human.

This is a High-sealed Storage Bottle (Brown) replacement o-ring for between the mouth and the cap.
Material : Perfluoro

该产品仅供研究使用。 不要将它管理给人类。

这是一个高密封存储瓶(棕色)替换O形圈,用于嘴和帽之间。
材质:全氟

品牌 产品编号 产品名称 规格
Wako 296-35691 High-sealed Storage Bottle, Brown, 1mL  高密封存储瓶,棕色,1mL 1个
Wako 296-34731 High-sealed Storage Bottle, Brown, 1ml 1 EA
Wako 297-34761 High-sealed Storage Bottle, Brown, 5ml 高度密封瓶(褐色)5ml 1 EA
Wako 291-34781 High-sealed Storage Bottle O-ring  高度密封保存瓶O环 2 pcs
Wako 293-35721 High-sealed Storage Bottle, Brown, 5mL  高密封存储瓶,棕色,5mL 1 个
Wako 294-34771 High-sealed Storage Bottle, Brown, 10ml  高度密封瓶(褐色)10ml 1 EA
Wako 296-35711 High-sealed Storage Bottle, Brown, 2mL  高密封存储瓶,棕色,2mL 1个

Wako和光纯药琼脂粉Agar

Wako和光纯药琼脂粉Agar

品牌 产品编号 产品名称 等级 规格 CAS No.
Wako 016-08722 Agar, Powder 琼脂粉 for Bacterial Culture Medium 25 g 9002-18-0
Wako 016-15812 Agar 琼脂 JIS Special Grade 25 g 9002-18-0
Wako 018-08721 Agar, Powder 琼脂粉 for Bacterial Culture Medium 100 g 9002-18-0
Wako 012-11872 Agar (Powder) 琼脂粉末 for Plant Culture Medium 25 g 9002-18-0
Wako 016-11875 Agar (Powder)  琼脂粉末 for Plant Culture Medium 500 g 9002-18-0
Wako 016-15817 Agar 琼脂 JIS Special Grade 10 kg 9002-18-0
Wako 010-08725 Agar, Powder 琼脂粉 for Bacterial Culture Medium 500 g 9002-18-0
Wako 010-15815 Agar 琼脂 JIS Special Grade 500 g 9002-18-0
Wako 018-15811 Agar 琼脂 JIS Special Grade 100 g 9002-18-0

Wako137-16-6 月桂酰肌氨酸钠

Wako137-16-6 月桂酰肌氨酸钠

英文名称:Sodium N-Dodecanoylsarcosinate
中文名称:月桂酰肌氨酸钠
品牌:Wako
品牌中文简称:和光纯药
CAS No.:137-16-6
储存条件:室温
纯度:95.0+% (Titration)

品牌 产品编号 产品名称 等级 规格 CAS No.
Wako 190-10383 Sodium N-Dodecanoylsarcosinate 月桂酰肌氨酸钠 Wako 1st Grade 50 g 137-16-6
Wako 192-10382 Sodium N-Dodecanoylsarcosinate 月桂酰肌氨酸钠 Wako 1st Grade 25 g 137-16-6
Wako 194-10381 Sodium N-Dodecanoylsarcosinate 月桂酰肌氨酸钠 Wako 1st Grade 100 g 137-16-6
Wako 198-14745 Sodium N-Dodecanoylsarcosinate 月桂酰肌氨酸钠 for Molecular Biology 500 g 137-16-6
Wako 196-10385 Sodium N-Dodecanoylsarcosinate 月桂酰肌氨酸钠 Wako 1st Grade 500 g 137-16-6

Wako 294-67001 TRAP/ALP stain kit TRAP/ALP双重染色试剂盒

Wako 294-67001 TRAP/ALP stain kit TRAP/ALP双重染色试剂盒

英文名称:TRAP/ALP stain kit
TRAP/ALP双重染色试剂盒
品牌:Wako
品牌中文简称:和光纯药
储存条件:-20℃
等级:for Pathology Research

品牌 产品编号 产品名称 等级 规格
Wako 294-67001 TRAP/ALP stain kit  TRAP/ALP双重染色试剂盒 for Pathology Research 60次

This product is for research use only. Do not administer it to human.
For enzyme activity staining of tartrate-resistant acid phosphatase and alkaline phosphatase

Normal bone metabolism is based on the balance between bone formation by osteoblasts and bone resorption by osteoclasts. When the balance is disturbed and bone resorption by osteoclasts is abnormally increased, bone mass is reduced leading to osteoporosis. Therefore, various researches have been carried out to understand the mechanism of osteoclast and osteoblast metabolism, and to utilize this knowledge for the treatment of the diseases and for the development of effective drugs.
Today, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) are known as marker enzymes for osteoblasts and osteoclasts, respectively, and these enzymes are used as one of the markers showing the presence of osteoblasts and osteoclasts in tissue sections or cultured cells.
This kit enables you to examine the state of differentiation of bone cells and the cell distribution in bone tissues by observation of the stained images of osteoblasts and osteoclasts in the tissues and cultured cells using ALP/TRAP enzyme activities in the tissue sections and cultured cells.

[Features]

In use, just mix two solutions for the preparation of the colorimetric substrate solution required for staining of acid phosphatase enzyme activity. (three solutions in case of the evaluation of tartrate-resistant acid phosphatase)

Simple steps for staining of alkaline phosphatase enzyme activity by using ALP substrate soln. (premixed).

Double staining can be performed, reddish-purple for active region of acid phosphatase and bluish brown for alkaline phosphatase.

Usable in cultured cells and bone tissue sections (GMA resin embedded section).

[Kit Contents]

Sodium tartrate soln. (x10) … 3 mL x 1 bottle

TRAP substrate soln. A … 30 mL x 1 bottle

TRAP substrate soln. N … 0.3 mL x 1 bottle

Nuclear stain soln. … 10 mL x 1 bottle

ALP substrate soln. (premixed) … 30 mL x 1 bottle

Wako 045-30741 核酸清除剂 DNA Cleaner

Wako 045-30741 核酸清除剂 DNA Cleaner

英文名称:DNA Cleaner
品牌:Wako
品牌中文简称:和光纯药
储存条件:2-10℃

核酸清除剂, 本品使用不需要分离管, 即可去除低分子DNA片断(≤50bp)
DNA Cleaner 本品不需要使用有机溶剂或离心管,35分钟内即可高效清除50bp 以下DNA 片断。另外,本品还可用于dsRNA 的纯化。最适合用于PCR 后的引物、引物对、dNTP、DNA 聚合酶的清除和限制性内切酶处理后的酶消除。
同时,利用乙醇沉淀法将ABI 公司BigDye Cycle Sequencing kit 扩增序列的DNA 样品纯化时,使用本品能得到清晰的序列结果。
特点
· 不需要使用分离管柱,也可纯化PCR扩增产物
· 高效浓缩500 ~ 2,500ng 的DNA
· 最快35分钟即可完成PCR后的核酸纯化

DNA Cleaner 使用用途

去除底物
去除底物二聚体
去除dNTP
DNA 纯化
测序用
标记反应后纯化
酶去除
操作时间
处理容量

100~10,000 bp
最短35分钟
与最初样品相同容量
与柱色谱法相比
DNA Cleaner
Q公司柱色谱法
Step 1
添加与DNA溶液等量的DNA Cleaner。
向PCR样品中添加5倍容量的结合液。此时,若混合物的颜色不为黄色,请添加3M醋酸钠。
Step 2
在室温下静置10分钟。
向离心管中添加Step 1.的样品。
Step 3
在室温下离心分离10分钟。
在室温下离心分离1分钟。
Step 4
除去上清液。添加70%乙醇。
除去滤液。添加洗涤液。
Step 5
在室温下离心分离1分钟。
在室温下离心分离1分钟。
Step 6
上清除去。干燥5分钟。
除去滤液。
Step 7
TE Buffer、灭菌水溶解
在室温下离心分离10分钟。
Step 8
FINISH
添加50μL析出液。静置1分钟。
Step 9
直接向分离管膜中添加析出液。
Step 10
在室温下离心分离10分钟。
Step 11
FINISH
所需时间
约35分钟
约10分钟
离心分离次数
2次
4次
DNA Cleaner 使用例
1)低分子DNA(50bp 以下)的除去
核酸清除剂 DNA Cleaner
条带1.:DNA Step Ladder (25-500bp)
条带2.:DNA Step Ladder (25-500bp)(DNA Cleaner 处理)
条带3.:DNA Step Ladder (25-500bp)(T 公司Kit)
条带4.:DNA Step Ladder (25-500bp)(Q 公司Kit)
图1 利用DNA Cleaner 去除50bp 以下的DNA 片断
使用本品可去除50bp及25bp的DNA 片断,可回收75bp 以上的DNA片断。与Q 公司Kit一样,可高效去除50bp 以下的DNA片断。
2)高分子DNA(200bp 以上)的回收
条带1.:DNA Ladder
条带2.:DNA Ladder (DNA Cleaner 处理)
条带3.:DNA Ladder (T 公司Kit)
条带4.:DNA Ladder (Q 公司Kit)
图2 利用DNA Cleaner 纯化高分子量DNA 片断
使用本品可高效回收200bp 以上的DNA片断。
3)少量DNA 的回收率比较
[使用DNA]3kb 质粒
[DNA 量检测法]检测OD260/OD280吸光度,估算已回收DNA的量。
(图3)利用DNA Cleaner 进行质粒DNA 添加回收实验
使用本品进行100 ~ 2,500ng 的DNA 回收实验,其结果比Q 公司Kit 更高效去除DNA,对500ng 以上的DNA 回収率达90% 以上。.
产品编号
品 名
包装
045-30741
DNA Cleaner
5mL
044-30711
25bp DNA Step Ladder (25-500bp)
500μL(100色带)
——除以上产品外,接受特殊规格定制规格,欢迎咨询!

Wako 291-59701 膳食纤维试剂盒,Dietary Fiber Assay Kit

Wako 291-59701 膳食纤维试剂盒,Dietary Fiber Assay Kit

英文名称:Dietary Fiber Assay Kit

中文名称:膳食纤维试剂盒

品牌:wako;

货号:291-59701
包装:100 tests

类别:糖苷

我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

本产品仅供研究用。不要把它给人类使用。

普洛斯基方法的一个简单的修正版本。与传统的Prosky方法相比,该方法具有较高的测量精度。此外,缩短了酶处理时间。

试剂盒组成:

    1. 耐热α-淀粉酶溶液1小瓶x 20 mL
    1. 蛋白酶溶液1小瓶x20 mL
    1. 酰胺葡萄糖苷酶溶液1小瓶x20 mL
  1. 硅藻土1小瓶x100克

测量轮廓

    1. 在MES/TRIS缓冲液中溶解的样品
    1. 耐热α-淀粉酶消化
    1. 蛋白酶和淀粉糖苷酶的消化
    1. 在pH6.3和60℃下持续30分钟
    1. 乙醇沉淀
    1. 休假1小时
    1. 过滤
    1. 乙醇和丙酮洗涤
    1. 一夜干
    1. Kjeldahl蛋白测定及灰分测定
  1. 总膳食纤维测定

原理

    1. 膳食纤维总量的测定
    1. 样品中的可消化组分,如淀粉和蛋白质,会被水解。
    1. 用α-淀粉酶、蛋白酶和淀粉糖苷酶,通过添加四倍体积的95%乙醇沉淀未分解的残余大分子,然后用含硅藻土的玻璃过滤坩埚捕获。
    1. 总膳食纤维含量是通过从玻璃过滤坩埚上捕获的残渣的干重中扣除蛋白质和灰分的含量来确定的。
    1. 可溶性膳食纤维和不溶性膳食纤维含量的分数分析
    1. 样品中的可消化组分,如淀粉和蛋白质,用α-淀粉酶、蛋白酶和淀粉糖苷酶水解,然后用含硅藻土的玻璃过滤坩埚过滤。
    1. 不溶性膳食纤维含量是通过在玻璃过滤坩埚上从不溶性残渣的干重中扣除蛋白质和灰分的含量来确定的。
    1. 同时,在滤液中加入四倍体积的95%乙醇,沉淀未分解的可溶性大分子,用含硅藻土的玻璃过滤坩埚捕集。可溶性膳食纤维含量是通过从玻璃过滤坩埚上沉淀残渣的干重中扣除蛋白质和灰分的含量来确定的。
    1. 总膳食纤维含量定义为不溶性膳食纤维含量和可溶性膳食纤维含量之和。
    1. 低分子可溶性膳食纤维的测定
      1. 用高效液相色谱法测定了特定的低分子可溶性膳食纤维,即使加入四倍体积的95%乙醇也不会沉淀。
      1. 用α-淀粉酶、蛋白酶和淀粉葡萄糖苷酶水解样品中的淀粉和蛋白质等可消化组分,再加入四倍体积的95%乙醇沉淀未分解的残余大分子。然后用含硅藻土的玻璃过滤坩埚过滤。
      1. 利用离子交换树脂去除滤液中的蛋白质、有机酸和无机盐,并将其应用于高效液相色谱。低分子可溶性膳食纤维含量是由样品的峰面积与葡萄糖的峰面积(内标)在色谱图上的比值来确定的。
      1. 同时,通过在玻璃过滤坩埚上从沉淀残渣的干重中扣除蛋白质和灰分的含量,确定了不溶性和可溶性大分子膳食纤维的含量。

总膳食纤维含量定义为低分子可溶性膳食纤维含量和不溶性和可溶性大分子膳食纤维含量之和。

This product is for research use only. Do not administer it to human.

A simple and modified version of Prosky’s method. Compared to conventional Prosky’s method, it assures high measurement accuracy. Furthermore, enzyme treatment time is shortened.

Kit Contents

    1. Thermostable alpha-Amylase Solution 1 vial x 20 mL
    1. Protease Solution 1 vial x 20 mL
    1. Amyloglucosidase Solution 1 vial x 20 mL
  1. Diatomaceous Earth 1 vial x 100 g

Measurement Outline

    1. Sample dissolved in MES/Tris buffer
    1. Digestion with thermostable alpha-amylase
    1. Digestion with protease and amyloglucosidase
    1. for 30 minutes at pH 6.3 and 60 degrees C
    1. Ethanol Precipitation
    1. Leave 1 hour
    1. Filtration
    1. Wash with ethanol and acetone
    1. Dry overnight
    1. Kjeldahl Protein Determination and Ash Determination
  1. Total Dietary Fiber Determination

Principle

      1. Determination of total Dietary Fiber Contents
      1. Digestible components in samples, such as starch and protein, are hydrolyzed
      1. with alpha-amylase, protease, and amyloglucosidase, precipitate undecomposed residual macromolecule by adding quadruple volume 95% Ethanol, and then trapped with a glass filter crucible containing diatomaceous earth.
      1. Total Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of trapped residue on a glass filter crucible.
      1. Fractional Analysis of Soluble Dietary Fiber Contents and Insoluble Dietary Fiber Contents
      1. Digestible components in samples, such as starch and protein, are hydrolyzed with alpha-amylase, protease, and amyloglucosidase, then filtrated with a glass filter crucible containing diatomaceous earth.
      1. Insoluble Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of trapped insoluble residue on a glass filter crucible.
      1. Meanwhile, add a quadruple volume 95% Ethanol into the filtrate, precipitate undecomposed residual soluble macromolecule, and trap with a glass filter crucible containing diatomaceous earth. Soluble Dietary Fiber Contents are determined by deducting weight of protein and ash content from the dry weight of the trapped precipitated residue on a glass filter crucible.
      1. Total Dietary Fiber Contents are defined as the sum of Insoluble Dietary Fiber Contents and Soluble Dietary Fiber Contents.
      1. Determination of Low-molecular Soluble Dietary Fiber
      1. Specific Low-molecular Soluble Dietary Fibers, which are not precipitated even by addition of a quadruple volume of 95% Ethanol, are determined by high-performance liquid chromatography.
      1. After digestible components such as starch and protein in samples are hydrolyzed with alpha-amylase, protease, and amyloglucosidase, the undecomposed residual macromolecule are precipitated by adding a quadruple volume of 95% Ethanol. Then filter it with a glass filter crucible containing diatomaceous earth.
      1. Proteins, organic acids and inorganic salts in the filtrate are removed with ion-exchange resin, and then the solution is applied to high-performance liquid chromatography. Low-molecular Soluble Dietary Fiber Content is determined by the ratio of the peak area of the sample with that of glucose (internal standard) on the chromatogram.
      1. Meanwhile, Insoluble and Soluble Macromolecule Dietary Fiber Content is determined by deducting weight of protein and ash content from the dry weight of the trapped precipitated residue on a glass filter crucible.

Total Dietary Fiber Contents is defined as sum of Low-molecular Soluble Dietary Fiber Contents and Insoluble and Soluble Macromolecular Dietary Fiber Contents.

Wako和光纯药特种化学-新型高纯度清洗剂NCW系列

Wako和光纯药特种化学-新型高纯度清洗剂NCW系列

上海金畔生物代理Wako和光纯药全线产品,欢迎新老客户访问Wako和光纯药官网或者咨询我们获取更多产品信息。

日本和光Wako特种化学-新型高纯度清洗剂NCW系列
NCW-1001、NCW-1002、NCW-1003和NCW-1004均是高纯度的清洗溶液,主要成分为非离子型表面活性剂,可以10 至100 倍稀释后使用,用于清洗硅晶片和液晶玻璃基板。

日本和光Wako特种化学-新型高纯度清洗剂NCW系列

NCW-1001和NCW-1002均是高纯度的清洗溶液,主要成分为非离子型表面活性剂,可以10 至100 倍稀释后使用,用于清洗硅晶片和液晶玻璃基板。

应用行业:半导体、LCD、LED、太阳能电池等。

日本和光Wako特种化学-新型高纯度清洗剂NCW系列

◆特点

1.金属杂质和颗粒杂质少,是高纯度的表面活性剂;

2.对脂肪和油具有更好的清洗效果;

3.良好的消泡性;

4.NCW-1001临界胶束浓度低,即使在低温条件下,用少量产品即可达到良好的清洗效果;

5.NCW-1002粘度低,操作方便,室温下与碱混合或者加热后使用;

6.对化学试剂具有良好的抗药性,产品稳定,对各种材料不会造成任何影响;

7.NCW-1001和NCW-1002不是有毒、有害或者危险物质;

◆应用法规,操作和保存的注意事项

法规:有毒有害物质控制法:没有限制;

消防法:没有限制

注意事项:如果眼睛或皮肤接触了试剂,请立即用大量清水清洗;若疼痛仍没减轻,请咨询医生;如吸入,请将吸入者移至室外,呼吸新鲜空气。

◆产品列表

产品编号 产品名称 产品规格
846-11311 NCW-1001清洗剂 1L
842-11416 NCW-1002清洗剂 1L

NCW-1001

Description

NCW-1001 is a high performance non-ionic surfactant which is used to manage the impurity of metal and particles. It has a high cleaning effect on fat and oil components which can even be enhanced by mixing it with alkaline.

NCW-1001 is environmentally friendly as natural alcohol is used as raw material, but nevertheless has a high and stable chemical resistance.

Chemical Name

Not relevant (mixture)

CAS No.: n/a
Molecular weight: n/a

Physical characteristics

Features Low temperature type

Main Applications

  • Additiv