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Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated
Description
NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.
Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Specifications
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Mannose |
Documents
- Lectins in Histochemistry, ELISA, and Western Blot Applications
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
Related Products
α-methylmannoside Catalog #: S-9005
Pisum Sativum Agglutinin (PSA), Biotinylated Catalog #: B-1055-5
Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Unconjugated Catalog #: L-1370-5
Concanavalin A (Con A), Biotinylated Catalog #: B-1005-5
Lens Culinaris Agglutinin (LCA), Biotinylated Catalog #: B-1045-5
Technical Information
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting Sugar: 400 mM α-methylmannoside