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Vector Labs 公司是世界著名的生物学检测试剂公司，七十年代其在全球首推的生物素-亲和素系统引领了生物学检测方法的重大革命，该系统被普遍视为目前最灵敏、最可靠与最有效的染色系统，并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。该系统仍在不断地发展，以满足广大研究人员的各种不同要求。Vector 公司还是世界上凝集素产品的主要供应商，并提供大量的新产品，如酶底物、神经元示踪剂及蛋白质、糖类与核酸的标记、分离与检测试剂。
Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U.
Description
Features:
- Affinity-purified, ultrapure, high affinity antibody
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
- Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
- Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
- Can be used for tissue and cell staining, ELISAs, and blots
- Supplied in a convenient ready-to-use prediluted, stabilized solution
Specifications
| Unit Size | 50 ml |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
| Concentration | Ready-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative. |
| Recommended Storage | 2-8 °C |
| Recommended Usage | Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl. |
| Target Species | Mouse |
| Conjugate | Biotinylated |
| Host Species | Goat |
| Format | Ready-to-Use |
Documents
- Safety Data Sheet
- Download CoA
- Datasheet
Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
Related Products
VECTASTAIN® Elite ABC-HRP Reagent, Peroxidase, R.T.U. Catalog #: PK-7100
Normal Goat Serum Blocking Solution, 2.5% Catalog #: S-1012-50
VECTASTAIN® ABC-AP Kit, Alkaline Phosphatase (Standard) Catalog #: AK-5000
VECTASTAIN® Elite ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-6100
Fluorescein Avidin DCS, Cell Sorting Grade Catalog #: A-2011-1
Texas Red® Avidin DCS, Cell Sorting Grade Catalog #: A-2016-1
VECTASTAIN® ABC-HRP Kit, Peroxidase (Standard) Catalog #: PK-4000
Normal Goat Serum Blocking Solution Catalog #: S-1000-20
Streptavidin, DyLight® 488 Catalog #: SA-5488-1
Streptavidin, DyLight® 549 Catalog #: SA-5549-1
Streptavidin, DyLight® 594 Catalog #: SA-5594-1
Streptavidin, DyLight® 649 Catalog #: SA-5649-1
Streptavidin, Peroxidase, R.T.U. Catalog #: SA-5704-100
Citations
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Biotinylated Goat Anti-Mouse IgG (H+L) is supplied in solution. With some exceptions the recommended dilution for most applications is 1:200.
(H+L) indicates the antibody recognizes both heavy and light chains.