Replacement Information
Replacement Information
INCP 000 10 (Protein Applications)
重要规格表
主要应用
西方蛋白印迹
描述
产品目录编号
IPVH00010
Replaces
INCP 000 10（蛋白质应用）
商名
Immobilon
描述
Immobilon-P 卷膜，PVDF，0.45μm，26.5cm x3.75m
背景信息
Overview
– Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
– 45 μm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
– Millipore’s Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
– the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
– 45 μm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
– When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
– Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Immobilon-PSQ Membrane:
– 0.2 μm pore size and a large internal structure
– Higher protein adsorption and sequencing yields than other membranes
– Recommended for blotting proteins less than 20 kDa
– Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane:
– The first transfer membrane optimized for fluorescence applications
– Extremely low background improves sensitivity of all fluorescence detection protocols
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
– Ideal for multiplexing and chemifluorescence applications
Immobilon PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits:
– Won’t crack, curl or fracture when cut
– Low background
– Superior staining capabilities
– Can be reprobed multiple times
Applications:
– Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
– Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
– Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
产品信息
检测方法
呈色 放射
相容的染色剂
考马斯亮蓝
酰胺黑
印度墨水
丽春红
胶体金
CPTS
甲苯胺蓝
透射显影
Sypro 宝石红
过滤器代码
IPVH
过滤器颜色
白色
Quality Level
MQ400
应用
应用
Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析
主要应用
西方蛋白印迹
生物信息
主结合机制
静电，疏水
BSA吸收性
215 μg/cm2
山羊IgG吸收性
294 μg/cm2
胰岛素吸收性
160 μg/cm2
可湿润性
疏水
理化信息
孔径
0.45 μm
尺寸
过滤器表面
平纹
过滤器尺寸
26.5 cm x 3.75 m
材料信息
化学
疏水性聚偏氟乙烯（PVDF）
包装信息
数量
1