ABSCIEX 研究试剂套装 A10663

产品介绍

ABSCIEX 研究试剂套装 A10663

 

仅供研究使用。 不适用于诊断程序。

包括 IgG 控制标准 1 包和所有化学品。
IgG 纯度和异质性测定是专为工业生物技术领域的研究人员开发的,他们正在开发和制造用于研究诊断和治疗的 IgG 试剂。 该测定被指定用于评估 IgG 试剂的纯度和异质性,无论是在降低状态还是非还原状态下。 该方法涉及在 SDS 存在的情况下对特定浓度的 IgG(还原和非还原的)进行热变性,并使用高分辨率毛细管凝胶电泳技术按大小分离这些蛋白质。 该检测将检测低至 0.1% 的杂质,并包括具有指定数量的非糖化重链的 IgG 对照组,以便在未知的运行之前测试该测定的分辨率和定量适宜性。

包括:

-分离毛细管 57 厘米 x 50
石英(数量 2)-SDS 凝胶分离缓冲液(专有配方)(140 毫升)-SDS 样品缓冲液 100 毫米三氯化碳pH 9.0/1% SDS(50 毫升)
-IgG 控制标准1毫克/毫升 SDS 样品缓冲液(1 毫升)
-内部标准 10 千达蛋白5毫克/毫升(0.4 毫升)
-酸性洗涤液 0.1 N HCL 100 毫升)
-基本清洗液 0.1 N Na
OH(100 毫升)-IgG 纯度和异质性测定应用指南(数量 1)
-试剂盒插入

此为机器翻译文本。

 

For research use only. Not for use in diagnostic procedures.

Includes IgG Control Standard 1-pack and all chemistries.
The IgG Purity and Heterogeneity Assay has been developed for researchers employed in industrial biotechnology who are developing and manufacturing IgG reagents for research diagnostic and therapeutic use. This assay has been specified to assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% and includes an IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns.

Includes:

– Separation Capillary 57 cm x 50 ?æm ID bare fused-silica (qty 2)
– SDS Gel Separation Buffer (proprietary formulation) (140 mL)
– SDS Sample Buffer 100 mM Tris-HCl pH 9.0/1% SDS (50 mL)
– IgG Control Standard 1 mg/mL in SDS sample buffer (1 mL)
– Internal Standard 10 kDa protein 5 mg/mL (0.4 mL)
– Acidic Wash Solution 0.1 N HCl (100 mL)
– Basic Wash Solution 0.1 N NaOH (100 mL)
– IgG Purity and Heterogeneity Assay Application Guide (qty 1)
– Kit Insert