VivaCell DMEM高糖(含谷氨酰胺,含丙酮酸钠)(C3113-0500)

【Product Description】

DMEM, High Glucose, with Sodium Pyruvate, with L-Glutamine is one of the most widely used modification of Eagle's medium.

DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs glucose per litre and was originally used to culture embryonic mouse cells.

DMEM high glucose is a further modification of original DMEM and contains 4500mgs glucose per litre. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines.

Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

【Composition】

Ingredients Mg/L
INORGANIC SALTS
Calcium chloride dihydrate 265.000
Ferric nitrate nonahydrate 0.100

Magnesium sulphate anhydrous

97.720

Potassium chloride 400.000
Sodium chloride 6400.000
Sodium dihydrogen phosphate anhydrous 109.000
AMINO ACIDS
Glycine 30.000

L-Arginine hydrochloride

84.000

L-Cystine dihydrochloride

62.570
L-Glutamine 584.000
L-Histidine hydrochloride monohydrate 42.000
L-Isoleucine 105.000
L-Leucine 105.000
L-Lysine hydrochloride 146.000
L-Methionine 30.000
L-Phenylalanine 66.000
L-Serine 42.000
L-Threonine 95.000
L-Tryptophan 16.000
L-Tyrosine Disodium Salt 103.790
L-Valine 94.000
VITAMINS
Choline chloride 4.000
D-Ca Pantothenate 4.000
Folic acid 4.000
Nicotinamide 4.000
Pyridoxal hydrochloride 4.000
Riboflavin 0.400
Thiamine hydrochloride 4.000
i-Inositol 7.200
OTHERS

D-Glucose

4500.000
Phenol red Sodium Salt 15.900
Sodium pyruvate 110.000
Sodium bicarbonate 3700.000

【Storage and Stability】

DMEM , High Glucose, with Sodium Pyruvate, with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.

As with any other liquid media formulations, deterioration of  liquid media may be recognized by any of the following characteristics, among others including:

(a). Color Change;

(b). Presence of clumping/flocculent debris/ granulation/ particulates precipitates or sediments;

(c). Insolubility;

(d). And/or decrease in expected performance parameters.

Any material described above should not be used and therefore discarded.

 

【Procedure】

1.Take a bottle from the defined storage conditions at 2-8°C and read the label.

2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.

3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.

4.Antibiotics may be added if desired.

 

【Quality Control】

DMEM , High Glucose, with Sodium Pyruvate, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.

 

【Precaution and Disclaimer】

For research use only, not for clinical diagnosis and treatment.