SARS-CoV-2 Omicron(XBB) Spike Gene ORF cDNA clone expression plasmid
基因
种属
SARS-CoV-2
基因长度
3810 bp
序列特征
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system.The translated amino acid sequence is identical with YP_009724390.1(T19I, del24-26, A27S, V83A, G142D, Y144del, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K).
产品特征
Full length Clone DNA of SARS-CoV-2 Omicron(XBB) Spike.
质粒
启动子
Enhanced CMV mammalian cell promoter
载体
pCMV3-untagged
限制性酶切位点
KpnI + XbaI(6.1kb+3.81kb)
测序引物
T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.