标签归档：cell

Millipore PICM0RG50 Millicell 插入式细胞培养皿, 30 mm, 亲水性 PTFE, 0.4 μm

发表于由

属性

物料

PTFE membrane (hydrophilic)
flat wells
polystyrene

质量水平

400

无菌性

sterile

特点

lid: no

包装

pack of 48

manufacturer/tradename

Millicell

参数

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

高度

5mm

直径

3.15cm

过滤器直径

30mm

过滤面积

4.2cm2

板尺寸

1wells

基质

Biopore

孔径

0.4μm pore size

input

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)

表面积

4.2cm2

binding type

low binding surface

detection method

fluorometric

运输

ambient

说明

一般描述

设备配置：6孔板，0.4μm孔径

使用Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。此外，Millicell嵌套板可实现对细胞单层的两侧都进行研究。

MillicellOrgantypic嵌套板：
– 用于实现高细胞活力以及针对三维外植体结构的出色研究
– 更低的高度使其能够放进标准的培养皿中
– Biopore（PTFE）膜可带来高活力—最长40天—以及极佳的跨膜氧气运输
– 膜是光学透明的，并经过优化可长期用于维持器官外植体

膜类型：
Biopore膜（亲水PTFE）
– 用于低蛋白结合、活细胞观察和免疫荧光应用

应用
细胞粘附、3D细胞培养、细胞生长、细胞分化、免疫细胞化学

干细胞类型：
小鼠胚胎干细胞
人胚胎干细胞
间充质干细胞
神经干细胞
造血干细胞
上皮细胞
胰腺干细胞
心脏干细胞
诱导性多能干细胞

包装

50 pk in Individually blister packaged

气泡袋独立包装

应用

亲水性PTFE细胞培养嵌套板，孔径为0.4 μm，用于6孔培养板，适用于细胞附着、细胞培养、细胞分化、荧光 & 低蛋白结合。

研究类别
细胞培养

Millipore PIHP01250 Millicell细胞培养小室， 12mm，聚碳酸酯，0.4 μm

发表于由

属性

物料

flat wells
polycarbonate membrane
polystyrene

质量水平

400

无菌性

sterile

特点

lid: no

包装

pack of 48

manufacturer/tradename

Millicell

参数

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

高度

10.5mm

过滤器直径

12mm

过滤面积

0.6cm2

板尺寸

1wells

工作体积

400μL

基质

Isopore

孔径

0.4μm pore size

input

sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type cardiac stem cell(s)

表面积

0.6cm2

binding type

Tissue Culture (TC)-treated surface

运输

ambient

说明

一般描述

设备配置：24孔板，0.4μm孔径

使用Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell 嵌套板使研究细胞单层的两侧成为可能。

Millicell 立式嵌套板：
-特别有助于细胞成长，为细胞研究提供了绝佳机会

膜类型：
等孔膜（聚碳酸酯）
-用于不依赖附着的细胞（无基质）的生长

应用
细胞附着、细胞生长、细胞分化、免疫细胞化学

Filter Code: PCF

Device Configuration: 24-well plate

包装

50 pk in Individually blister packaged

独立包装

Millipore PTHT24H48 Millicell Hanging Cell Culture Insert, P

发表于由

属性

物料

PET membrane
flat wells
polystyrene housing

质量水平

200

无菌性

sterile; γ-irradiated

包装

pack of 48ea (individually blister packaged)

参数

max. temp. at 50 °C

过滤器直径

6.5mm

过滤面积

0.3cm2

孔最大体积

400μL

工作体积

200μL

孔径

0.4μm

表面积

0.3cm2

binding type

Tissue Culture (TC)-treated surface

说明

一般描述

Replacement for MCHT24H48

MillicellHanging Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an 0.4 μm membrane pore size and fits in most 24-well plastic tissue culture plates.

In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicelltissue culture inserts, cells can access media from both their apical and basolateral sides. As a result, cell growth, structure, and function more closely mimic what occursin vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

应用

Cell Proliferation

Cell Surface Receptors

Co-Culture

Epithelial Cell Growth

Feeder Layers

Fluorescent Detection /Immunohistochemistry

In VitroToxicology

Microbial Attachment

Polarized Protein Secretions

Polarized Uptake

Transport/Permeability

法律信息

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Millipore PHCC360500 Scepter 3.0 Cell Counter Sensors, 60um, 500pk

发表于由

属性

特点

50μL (sample volumn)

包装

pkg of 500ea

manufacturer/tradename

Scepter

参数

10000 particles/mL particle concentration (500000 particles/mL)

technique(s)

cell counting: suitable

粒径

6-36μm

相容性

Scepter for use with cells sized 8.0–25.0 μm

Not compatible with Scepter 2.0 Instruments

运输

ambient

说明

一般描述

The Scepter 3.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter 3.0 cell counter′s screen displays:

Cell concentration

Average cell size

Average cell volume

Histogram of size or volume distribution

This system is intended for research use only and has been tested with cell types representative of those in use today.

特点和优势

The Scepter 3.0 sensor is engineered with a micro-fabricated, cell-sensing zone that enables discrimination by cell size and cell volume – down to sub-micron and sub-picoliter resolution.

Coupled with precision liquid handling channels and electronics, the Scepter sensor accurately and reliably provides cell population statistics

其他说明

Not compatible with Scepter 2.0 products

Millipore PSHT010R5 Millipore,PSHT010R5,Millicell-24 细胞培养嵌套板， 聚碳酸酯, 0.4 μm

发表于由

属性

物料

flat wells
polycarbonate membrane
polystyrene

质量水平

400

无菌性

sterile

特点

lid

manufacturer/tradename

Millicell

参数

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

高度

26.2mm

长度

128mm

宽度

84.8mm

过滤器直径

9.5mm

过滤面积

0.7cm2

板尺寸

24wells

工作体积

400μL

基质

Isopore

孔径

0.4μm pore size

input

sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells

binding type

Tissue Culture (TC)-treated surface

运输

ambient

收起

说明

一般描述

Millicell-24聚碳酸酯细胞培养插入板是理想的细胞附着、细胞培养、细胞分化、药物转运渗透性。

Millicell装置可使用0.4 μm的聚碳酸酯薄膜(PCF)膜，为高完整性细胞单层膜的生长和可持续性提供最佳条件。

采用顶端辅助，便于移液和基底外侧进入。

包装

5 ea in Bulk packed

整批包装

组分

24孔细胞培养板，单孔饲养板，盖子

Millipore PIXP01250 Millicell细胞培养小室，12mm，聚碳酸酯，12 μm

发表于由

属性

物料

polycarbonate membrane
polystyrene housing

质量水平

400

无菌性

sterile; γ-irradiated

包装

pack of 50

manufacturer/tradename

Millicell

参数

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

高度

10.5mm

过滤器直径

12mm

过滤面积

0.6cm2

尺寸

24wells

表面积

0.6cm2
0.6cm2

工作体积

0.6mL

基质

Isopore

孔径

12.0μm pore size

吸附类型

Tissue Culture (TC)-treated surface

运输

ambient

收起

比较类似商品

查看完整比较结果

此商品

PITP01250

PIHP03050

PI8P01250

Millipore

PIXP01250

Millicell细胞培养小室，12mm，聚碳酸酯，12 μm

查看价格及是否有存货

Millipore

PITP01250

Millicell细胞培养小室，12mm，聚碳酸酯，3.0 μm

查看价格及是否有存货

Millipore

PIHP03050

Millicell细胞培养小室，30 mm，聚碳酸酯，0.4 μm

查看价格及是否有存货

Millipore

PI8P01250

Millicell细胞培养小室，12mm，聚碳酸酯，8.0 μm

查看价格及是否有存货

material

polycarbonate membrane, polystyrene housing

material

polycarbonate membrane, polystyrene housing

material

polycarbonate membrane, polystyrene housing

material

polycarbonate membrane, polystyrene housing

sterility

sterile; γ-irradiated

sterility

sterile; γ-irradiated

sterility

sterile

sterility

sterile; γ-irradiated

packaging

pack of 50

packaging

pack of 50

packaging

pack of 50

packaging

pack of 50

manufacturer/tradename

Millicell

manufacturer/tradename

Millicell

manufacturer/tradename

Millicell

manufacturer/tradename

Millicell

size

24wells

size

24wells

size

6wells

size

24wells

working volume

0.6mL

working volume

0.6mL

working volume

1.5mL

working volume

0.6mL

说明

一般描述

设备配置：24孔板，12μm孔径

使用Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可以研究细胞单层的两侧。

Millicell立式嵌套板：
-特别有助于细胞成长，为细胞研究提供了绝佳机会
-提供Biopore（PTFE）膜、MF-Millipore（混合纤维素酯）膜和聚碳酸酯膜

膜类型：
等孔膜（聚碳酸酯）
-用于不依赖附着的细胞（无基质）的生长

应用
细胞附着、细胞生长、细胞分化、免疫细胞化学

应用

研究类别
细胞培养

聚碳酸酯细胞培养嵌套板，孔径 12.0 μm，用于24孔板的细胞附着、细胞培养、细胞分化、药物转运 & 渗透性。

包装

气泡袋独立包装

法律信息

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Millipore PSHT004R5 聚碳酸酯，0.4um,Millicell-96 细胞培养嵌套板

发表于由

属性

物料

flat wells
polycarbonate membrane
polystyrene

质量水平

400

无菌性

sterile

特点

lid

manufacturer/tradename

Millicell

参数

75-100 μL sample volume

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

高度

26.2mm

长度

128mm

宽度

84.8mm

过滤面积

0.11cm2

板尺寸

96wells

工作体积

75μL

基质

Isopore

孔径

0.45μm pore size

input

sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type cardiac stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)

binding type

Tissue Culture (TC)-treated surface

运输

ambient

收起

说明

一般描述

Millicell 细胞培养嵌套板是一种专利装置，设计用于支持内皮和上皮细胞系（包括 CaCo-2）的细胞生长和分化。

Millicell 装置有 0.4 μm PCF 和多种 PET 薄膜孔径。培养板给用户提供了最大方便性，有许多特点，例如更加方便移液的上侧辅助孔以及下侧接触孔。为了降低污染细胞单层的风险，薄膜板有支脚，使板子在从饲养托盘拆下时高于工作表面。泪滴形状的容纳孔消除了在组装板子时产生的气泡。

Millicell 培养板经优化用于生长细胞和维持细胞单层的高度完整性。Millicell培养板薄膜上生长的细胞的生长情况比在塑料上好，因为细胞从上侧和下侧均获得营养。更加密切地模拟了体内细胞生长和功能。Millicell 培养板设计用于分析及细胞生长，亦可用作手动或自动细胞接种、饲养和清洗系统。

使用这一完善的系统，您可以在一个以薄膜做底的培养板上生长、饲养和分析细胞。培养板可以与单槽或多孔饲养托盘配用。在进行转运分析时，只需将培养板转换成分析用的多孔转运托盘。

这种流线型设计增强了下列功能：

-接种和饲养系统

-自动液体处理系统和跨距移液

-下侧和上侧均可接触到细胞

-经上皮电阻（TEER）测量系统

滤器编码：PCF

包装

1 ea in Individually blister packaged

5 ea in Bulk packed

大包装

组分

96孔细胞培养板（5），单槽饲养托盘（5），盖子（5）

外形

色码：透明

其他说明

自动化兼容性：无
5支装不包括单孔托盘产品PSSW010R5

Millipore PHCC340500 Scepter 3.0 Cell Counter Sensors, 40um, 500pk

发表于由

属性

特点

50μL (sample volumn)

包装

pkg of 500ea

manufacturer/tradename

Scepter

参数

50000 particles/mL particle concentration (1500000 particles/mL)

technique(s)

cell counting: suitable

粒径

4-18μm

相容性

Scepter for use with cells sized 5.0–15.0 μm

Not compatible with Scepter 2.0 Instruments

运输

ambient

说明

一般描述

The Scepter 3.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter 3.0 cell counter′s screen displays:

Cell concentration

Average cell size

Average cell volume

Histogram of size or volume distribution

This system is intended for research use only and has been tested with cell types representative of those in use today.

特点和优势

The Scepter 3.0 sensor is engineered with a micro-fabricated, cell-sensing zone that enables discrimination by cell size and cell volume – down to sub-micron and sub-picoliter resolution.

Coupled with precision liquid handling channels and electronics, the Scepter sensor accurately and reliably provides cell population statistics

其他说明

Not compatible with Scepter 2.0 products

Hyclone SH31027.03CN DPM CELL BOOST 7b 25L

发表于由

目前补料批培养已成为蛋白药物生产的最常用方式。在批培养过程中,适当添加补料可明显提高产量。HyClone Cell boost 系列补料平台包含 cell boost1-6 和 7a/7b。每种补料均为代谢途径设计开发,为不同的用途提供相应的营养需求。在实际应用中,可根据不同细胞的生理状况,进行同的搭配组合,以达到最佳的产量。补料培养基均为化学成分限定,并不含动物源成分的培养基(干粉)

Hyclone SH30584.05 Cell Boost 1 (R05.2) Supplement without L-Glutamine

发表于由

本产品为无血清培养基工程添加剂 Cell Boost 1 (R05.2) 。CellBoost添加剂是化学合成的添加剂，无动物源成分， 旨在为补料分批培养或培养基定制提供营养。

Cell Boost 1是一种提供诸如氨基酸、维生素和葡萄糖等营养物质的添加剂。其目的是通过多种维生素补给方式提高产量。

Cell Boost 1已经在多种细胞系中得到检验，包括CHO和HEK 293细胞。

产品名称

氨基酸

维生素

葡萄糖

微量元素

生长因子

次黄嘌呤/胸苷

脂类

胆固醇

CellBoost 1(R06.2)

X

X

X

CellBoost 2(R15.4)

X

X

X

CellBoost 3(JM3.5)

X

X

X

X

X

CellBoost 4(PS307)

X

X

X

X

X

X

X

CellBoost 5(CN-F)

X

X

X

X

X

X

X

X

CellBoost 6(CN-T)

X

X

X

X

X

X

X

X

LS1000

X

LS250

X

X