Lucigen | LGC代理,FailSafe Enzyme Mix,部分现货


FailSafe Enzyme Mix

PCR Enzyme Mix, individual component of the FailSafe PreMix Selection Kit for PCR optimization, and the bundled post – optimization FailSafe PCR System.

Product Details

The PCR Enzyme Mix is a blend of thermostable DNA polymerases containing a 3′→5′ proofreading enzyme for high fidelity. Supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 0.5% Tween®20, 0.5% NP40, and 1 mM dithiothreitol.

Choose from 2 different sizes of FailSafe PCR Enzyme Mix, 100 Units (40 uL) and 1000 Units (400 uL).

Before purchasing we strongly recommend first optimising your PCR conditions using the FailSafe PCR PreMix Selection Kit. If optimization is complete, you can proceed with the bundled and affordable post optimisation FailSafe PCR System.

The “failsafe” nature is guaranteed when using all of the FailSafe components.

Key features

  • Unique blend of thermostable enzymes
  • 3′→5′ proofreading enzyme
  • High fidelity at least 3 times higher than Taq DNA polymerase

Select a Size

Please select a Size

Lucigen | LGC, Biosearch Technologies主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、聚合酶及E.cloni®感受态细胞及蛋白表达系统等。

Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。此外,Lucigen还是全球科学家青睐的Epicentre品牌产品的供应商。在2018年,Lucigen被LGC收购,从而加强了其在基因组市场的试剂供应。Lucigen的产品和服务已经覆盖了临床诊断、药物开发、合成生物学和基因编辑等多个领域。更多产品信息欢迎访问Lucigen | LGC, Biosearch Technologies官网。

Lucigen | LGC代理,LavaLAMP DNA Master Mix,部分现货


LavaLAMP DNA Master Mix

Sensitive and Fast DNA LAMP in an easy-to-use Master Mix format.

Product Details

Both LavaLAMP DNA Master Mix kits contain: LavaLAMP DNA Master Mix, DNA Positive Control LAMP Primer Mix, and DNA Positive Control. The LavaLAMP DNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA.

Key features

  • Loop-mediated isothermal amplification (LAMP): Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory.
  • Master Mix Format: Simplifies reaction setup and minimises optimisation required to produce the best assay results.
  • High Thermal Stability: Highly thermostable enzyme (up to 90 °C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results.
  • Elevated LAMP Reaction Temperature (68-74 °C):Improves primer specificity and reduces background amplification depending on the DNA target.
  • Freeze-dry Compatible: Enables generation of room temperature stable test kits through lyophilisation.

Select a Format

Please select a Format

Lucigen | LGC, Biosearch Technologies主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、聚合酶及E.cloni®感受态细胞及蛋白表达系统等。

Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。此外,Lucigen还是全球科学家青睐的Epicentre品牌产品的供应商。在2018年,Lucigen被LGC收购,从而加强了其在基因组市场的试剂供应。Lucigen的产品和服务已经覆盖了临床诊断、药物开发、合成生物学和基因编辑等多个领域。更多产品信息欢迎访问Lucigen | LGC, Biosearch Technologies官网。

Lucigen | LGC代理,LavaLAMP RNA Master Mix,部分现货


LavaLAMP RNA Master Mix

Sensitive RNA detection with this easy-to-use RNA LAMP (RT-LAMP) Master Mix.

Product Details

Both LavaLAMP RNA Master Mix kits contain: LavaLAMP RNA Master Mix, RNA Positive Control LAMP Primer Mix, and RNA Positive Control. The LavaLAMP RNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

Key features

  • Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation
  • Master Mix Format: Streamlines reaction setup while reducing potential handling errors
  • Minimal Optimisation: Focuses optimisation on the two critical reaction parameters – primer design and reaction temperature

  • Lyophilisation-ready: Avoids redesign of assays to remove components known to inhibit lyophilisation all components are lyophilisation-compatible

Select a Format

Please select a Format

Lucigen | LGC, Biosearch Technologies主要开发各类用于基因克隆的试剂盒及相关产品,包括:CloneSmart®平端克隆试剂盒、BigEasy®线性克隆系统、pEZSeq™平端克隆试剂盒、聚合酶及E.cloni®感受态细胞及蛋白表达系统等。

Lucigen公司凭借其独到的产品技术,过硬的产品质量,良好的产品服务赢得了全球广大用户的信赖。此外,Lucigen还是全球科学家青睐的Epicentre品牌产品的供应商。在2018年,Lucigen被LGC收购,从而加强了其在基因组市场的试剂供应。Lucigen的产品和服务已经覆盖了临床诊断、药物开发、合成生物学和基因编辑等多个领域。更多产品信息欢迎访问Lucigen | LGC, Biosearch Technologies官网。

RT-PCR Mix for qPCR(预混型逆转录试剂盒)

RT-PCR Mix for qPCR(预混型逆转录试剂盒)

货号:R1031   规格:100次(20 µl反应体系)

 

产品说明

RT-PCR Mix for qPCR是预混型逆转录反应试剂盒,操作简便,可快速高效完成逆转录反应。其中Enzyme Mix含有高效逆转录酶和热敏型DNase,一步完成基因组DNA的清除以及RNA的逆转录反应,RT Buffer包含逆转录反应所需的其他组分,无需分步多次加样,降低了样品污染的风险。同时,本试剂盒配备了Control Mix,用于阴性对照反应,检验RNA模板中是否有基因组DNA残留。本试剂盒的逆转录产物对染料法和探针法荧光定量PCR反应均有很好的兼容性,可进行精准的基因表达分析。

 

产品组分

组分

R1031100次)

Enzyme   Mix

100   μl

5X   RT Buffer

400 μl

Control   Mix

20   μl

RNase-free   ddH2O

1   ml × 2

 

保存条件

各组分均-20℃保存,并避免反复冻融。

 

适用范围

第一链cDNA 合成

 

注意事项

本品含有热敏型DNase,请于冰上使用本品各组分。

为保证成分均匀,请将Enzyme Mix5X RT BufferControl Mix分别瞬时离心、吹打混匀后再吸取使用。

在操作过程中,注意防止引入RNase导致RNA降解。RNA最好保存于-70℃,避免反复冻融。

l cDNA产物应置于-20℃或以下保存,避免反复冻融。

 

操作步骤

1. 配制反应体系

按以下体系,在冰浴的无菌无酶离心管中配制反应混合液:

组分

用量

5X   RT Buffer

4 μl

Enzyme   Mix

1 μl

RNA

1pg-1µg

RNase-free   ddH2O

补足至20 μl 

 

阴性对照反应(可选)

即不加逆转录酶的反应,产物用于在qPCR中作为阴性对照,检验模板RNA中是否有基因组DNA残留

按以下体系,在冰浴的无菌无酶离心管中配制反应混合液:

组分

用量

5X   RT Buffer  

4 μl

Control   Mix

1 μl

RNA

1pg-1µg

RNase-free   ddH2O

补足至20 μl 

 

2. 进行逆转录反应

将上述混合液吹打混匀,瞬时离心收集至管底。按以下程序,在PCR仪中进行反应:

温度

时间

50

15   min

85

5   sec

 

3. qPCR反应及cDNA保存

cDNA产物可稀释后立即进行染料法或探针法qPCR反应。若使用未稀释的cDNA原液,则体积不能超过反应体系的1/10,以避免抑制反应效率。长期保存cDNA产物应于-20℃或以下环境中,并避免反复冻融。