标签归档：antibody

SIGMA A2052-100UL 抗GABA抗体 兔抗

发表于由

属性
生物来源
rabbit

质量水平
200

偶联物
unconjugated

抗体形式
affinity isolated antibody

antibody product type
primary antibodies

克隆
polyclonal

形式
buffered aqueous solution

species reactivity
rat, Drosophila, wide range

包装
antibody small pack of 25 μL

technique(s)
dot blot: 1:10,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum

运输
dry ice

储存温度
20°C

Gene Information

rat … Gabra1(29705)

说明
一般描述
抗GABA抗体是使用GABA-BSA作为兔的免疫原进行生产的。通过免疫特异性纯化方法从抗血清中分离抗体。抗原特异性亲和分离可去除几乎所有兔血清蛋白，包括不与GABA特异性结合的免疫球蛋白。
GABA（γ-氨基丁酸）是存在于从原核生物到脊椎动物的多种生物体内的多功能分子。它存在于非神经结构中，如外周神经和内分泌系统。[1]谷氨酸脱羧酶（GAD）催化谷氨酸发生脱羧反应，形成GABA。
免疫原
γ-氨基丁酸(GABA)-BSA结合物。
应用
使用兔抗GABA抗体通过免疫荧光法检测从E19天大鼠胚胎大脑神经细胞中GABA的表达。用抗GABA抗体和两种抗GAD抗体进行三重IF染色。通过免疫组织化学检测方法，分析从各种动物（包括大鼠、小鼠、兔、豚鼠和蜥蜴）大脑皮层分离的细胞中GABA的表达。使用以1:1000比例溶于0.01M PBS（pH 7.4）+0.5％triton-x100溶液中的兔抗GABA抗体进行IHC分析。
兔抗GABA抗体可用于大鼠海马组织的电子显微镜分析，稀释度为1:4000。该抗体还被用于果蝇和美洲大蠊脑细胞的免疫细胞化学分析，稀释度范围为1:100-1:750。
生化/生理作用
GABA（γ-氨基丁酸）是中枢神经系统的主要抑制性神经递质，并且是一些外周组织的神经调节剂。它能够控制心率、血压和呼吸过程。GABA通过与GABA/苯二氮受体发生相互作用并打开氯离子通道而促进神经元抑制。在斑点杂交印迹试验中，兔抗GABA抗体表现出与GABA和GABA-钥孔虫戚血兰素（KLH）发生阳性结合，并与牛血清白蛋白（BSA）发生阴性结合。
外形
溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有1% BSA和15 mM 叠氮化钠。
储存及稳定性
若需连续使用，可在2-8°C下保存最长一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、 体外 诊断用途、 离体 或 体内 治疗用途或任何类型的消费或应用于人类或动物。

SIGMA MAB1217-I Anti-Fmc-7 (B-Cell Lymphocyte Marker), clone Fmc-7 Antibody

发表于由

属性
生物来源
mouse

质量水平
100

抗体形式
purified immunoglobulin

antibody product type
primary antibodies

克隆
Fmc-7, monoclonal

species reactivity
human

technique(s)
flow cytometry: suitable
western blot: suitable

同位素/亚型
IgMκ

运输
wet ice

Gene Information

human … MS4A1(931)

说明
一般描述
B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
免疫原
HRIK [human B-lymphoblastoid cell] corresponding to human Fmc-7 (B-Cell Lymphocyte Marker).
应用
Flow Cytometry Analysis: 4.0 μg of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in peripheral blood mononuclear cells.
This Anti-Fmc-7 (B-Cell Lymphocyte Marker) antibody is validated for use in WB, FC for the detection of Fmc-7 (B-Cell Lymphocyte Marker).
质量
Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 2.0 μg/mL of this antibody detected Fmc-7 (B-Cell Lymphocyte Marker) in 10 μg of THP-1 cell lysate.
目标描述
~105 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Ammonium Sulfate
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing 0.1M Tris, 10mM Glycine, 0.5M NaCl, and 0.1% Sodium Azide.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Anti-Swine IgG (H+L) Antibody（5210-0173）

发表于由

KPL Antibodies & Conjugates

Anti-Swine IgG (H+L) Antibody

Anti-Swine IgG (H+L) Antibody

货号：5210-0173

规格：1mg

品牌：seracare

Anti-Swine IgG (H+L) Antibody（5210-0173）


Material Number5210-0173
Size1 mg
Inventory StatusIn Stock
UnitEA


    DETAILS
Affinity-purified polyclonal antibody to swine IgG, both heavy and light chains (whole IgG), made in goat. Product is in lyophilized form. Each lot is tested to assure specificity and lot-to-lot consistency using an in-house ELISA assay.

Reference Number: 01-14-06

Mouse monoclonal eGFP antibody, clone OTI5A2小鼠单克隆 eGFP 抗体，克隆 OTI5A2

发表于由
Product Data
Product Name Mouse monoclonal eGFP antibody, clone OTI5A2
Clone Name OTI5A2 (formerly 5A2)
Applications WB
Target eGFP
Host Mouse
Clonality Monoclonal
Immunogen A full length eGFP tagged recombinant protein expressed in HEK293T cell
Isotype IgG1
Formulation PBS (pH 7.3) containing 1%BSA, 50% glycerol and 0.02% sodium azide
Concentration 0.7mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.
Predicted Protein Size 26.9 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice ,Caballero, B;Bourdenx, M;Luengo, E;Diaz, A;Sohn, PD;Chen, X;Wang, C;Juste, YR;Wegmann, S;Patel, B;Young, ZT;Kuo, SY;Rodriguez-Navarro, JA;Shao, H;Lopez, MG;Karch, CM;Goate, AM;Gestwicki, JE;Hyman, BT;Gan, L;Cuervo, AM;, Nature communications 2021 ,PubMed ID 33854069 [EGFP]
A Small Cysteine-Free Protein Acts as a Novel Regulator of Fungal Insect-Pathogenic Lifecycle and Genomic Expression ,Mou, YN;Fu, B;Ren, K;Ying, SH;Feng, MG;, mSystems 2021 ,PubMed ID 33758028 [EGFP]
Acetylation of ELF5 suppresses breast cancer progression by promoting its degradation and targeting CCND1 ,Li, X;Li, S;Li, B;Li, Y;Aman, S;Xia, K;Yang, Y;Ahmad, B;Wu, H;, NPJ precision oncology 2021 ,PubMed ID 33742100 [EGFP]
Strategies to prevent cleavage of the linker region between ligand-binding repeats 4 and 5 of the LDL receptor ,Strøm, TB;Bjune, K;da Costa, LT;Leren, TP;, Hum. Mol. Genet. 2019 ,PubMed ID 31332430 [EGFP]
MACROD2 Haploinsufficiency Impairs Catalytic Activity of PARP1 and Promotes Chromosome Instability and Growth of Intestinal Tumors ,Sakthianandeswaren, A;Parsons, M;Mouradov, D;MacKinnon, RN;Catimel, B;Liu, S;Palmieri, M;Love, CG;Jorissen, RN;Li, S;Whitehead, L;Putoczki, TL;Preaudet, A;Tsui, C;Nowell, CJ;Ward, RL;Hawkins, NJ;Desai, J;Gibbs, P;Ernst, M;Street, I;Buchert, M;Sieber, OM;, Cancer Discov 2018 ,PubMed ID 29880585 [GFP]
Antagonizing STAT5B dimerization with an osmium complex ,Liu, LJ;Wang, W;Kang, TS;Liang, JX;Liu, C;Kwong, DW;Wong, VK;Ma, DL;Leung, CH;, Sci Rep 2016 ,PubMed ID 27853239 [EGFP]
N-cadherin coordinates AMP kinase-mediated lung vascular repair ,Jian, MY;Liu, Y;Li, Q;Wolkowicz, P;Alexeyev, M;Zmijewski, J;Creighton, J;, Am. J. Physiol. Lung Cell Mol. Physiol 2016 ,PubMed ID 26545901 [EGFP]
HuR antagonizes the effect of an intronic pyrimidine-rich sequence in regulating WT1+/- KTS isoforms ,Li, H;Hou, S;Hao, T;Azam, S;Liu, C;Shi, L;Lei, H;, RNA biology Dec 2015 ,PubMed ID 26512748 [eGFP]
CD14 dependence of TLR4 endocytosis and TRIF signaling displays ligand specificity and is dissociable in endotoxin tolerance ,Rajaiah, R;Perkins, DJ;Ireland, DD;Vogel, SN;, Proc. Natl. Acad. Sci. U.S.A. Jul 2015 ,PubMed ID 26106158 [eGFP]
Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export ,Haixin Lei, Bo Zhai, Shanye Yin, Steve Gygi, and Robin Reed, Nucleic Acids Res., Dec 2012; 10.1093/nar/gks1314. ,PubMed ID 23275560 [eGFP]

HA mouse monoclonal antibody, clone CB051；HA小鼠单克隆抗体，克隆CB051

发表于由
 Product Data
Product Name HA mouse monoclonal antibody, clone CB051
Clone Name CB051
Applications WB
Target HA tag
Host Mouse
Clonality Monoclonal
Immunogen Monoclonal antibody is produced by immunizing mice with a synthetic peptide from influenza hemaglutinin coupled to KLH
Isotype IgG1
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
VRK2 is involved in the innate antiviral response by promoting mitostress-induced mtDNA release ,He, WR;Cao, LB;Yang, YL;Hua, D;Hu, MM;Shu, HB;, Cellular & molecular immunology 2021 ,PubMed ID 33785841 [HA]
WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation ,Xiong, Y;Yuan, L;Chen, S;Xu, H;Peng, T;Ju, L;Wang, G;Xiao, Y;Wang, X;, Cell Death Dis 2020 ,PubMed ID 32678075 [HA]
Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis ,Zhong, L;Hu, MM;Bian, LJ;Liu, Y;Chen, Q;Shu, HB;, Cell Discov 2020 ,PubMed ID 32351706 [HA]
Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners ,Gallardo-Vara, E;Ruiz-Llorente, L;Casado-Vela, J;Ruiz-Rodríguez, MJ;López-Andrés, N;Pattnaik, AK;Quintanilla, M;Bernabeu, C;, Cells 2019 ,PubMed ID 31540324 [HA]
Virus-induced accumulation of intracellular bile acids activates the TGR5-β-arrestin-SRC axis to enable innate antiviral immunity ,Hu, MM;He, WR;Gao, P;Yang, Q;He, K;Cao, LB;Li, S;Feng, YQ;Shu, HB;, Cell Res. 2019 ,PubMed ID 30651583 [HA]
The NLRP6 Inflammasome Recognizes Lipoteichoic Acid and Regulates Gram-Positive Pathogen Infection ,Hara, H;Seregin, SS;Yang, D;Fukase, K;Chamaillard, M;, Cell 2018 ,PubMed ID 30392956 [HA]
Human Cytomegalovirus Protein UL31 Inhibits DNA Sensing of cGAS to Mediate Immune Evasion ,Huang, ZF;Zou, HM;Liao, BW;Zhang, HY;Yang, Y;Fu, YZ;Wang, SY;Luo, MH;Wang, YY;, Cell Host Microbe 2018 ,PubMed ID 29937271 [HA]
CDK5 Inhibition Resolves PKA/cAMP-Independent Activation of CREB1 Signaling in Glioma Stem Cells ,Mukherjee, S;Tucker-Burden, C;Kaissi, E;Newsam, A;Duggireddy, H;Chau, M;Zhang, C;Diwedi, B;Rupji, M;Seby, S;Kowalski, J;Kong, J;Read, R;Brat, DJ;, Cell Rep 2018 ,PubMed ID 29742423 [HA]
Poly(ADP-ribose) Polymerase 1, PARP1, modifies EZH2 and inhibits EZH2 histone methyltransferase activity after DNA damage ,Caruso, LB;Martin, KA;Lauretti, E;Hulse, M;Siciliano, M;Lupey-Green, LN;Abraham, A;Skorski, T;Tempera, I;, Oncotarget 2018 ,PubMed ID 29535829 [HA]
Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition ,Collart, C;Smith, JC;Zegerman, P;, Dev. Cell 2017 ,PubMed ID 28697335 [HA]
Variants in TRIM22 That Affect NOD2 Signaling Are Associated With Very-Early-Onset Inflammatory Bowel Disease ,Li, Q;Lee, CH;Peters, LA;Mastropaolo, LA;Thoeni, C;Elkadri, A;Schwerd, T;Zhu, J;Zhang, B;Zhao, Y;Hao, K;Dinarzo, A;Hoffman, G;Kidd, BA;Murchie, R;Al Adham, Z;Guo, C;Kotlarz, D;Cutz, E;Walters, TD;Shouval, DS;Curran, M;Dobrin, R;Brodmerkel, C;Snapper, SB;Klein, C;Brumell, JH;Hu, M;Nanan, R;Snanter-Nanan, B;Wong, M;Le Deist, F;Haddad, E;Roifman, CM;Deslandres, C;Griffiths, AM;Gaskin, KJ;Uhlig, HH;Schadt, EE;Muise, AM;, Gastroenterology 2016 ,PubMed ID 26836588 [HA]
EZH1/SUZ12 complex positively regulates the transcription of NF-?B target genes via interaction with UXT ,Su, SK;Li, CY;Lei, PJ;Wang, X;Zhao, QY;Cai, Y;Wang, Z;Li, L;Wu, M;, J. Cell. Sci. Apr 2016 ,PubMed ID 27127229 [HA]
The Key Regulator for Language and Speech Development, FOXP2, is a Novel Substrate for SUMOylation ,Meredith, LJ;Wang, CM;Nascimento, L;Liu, R;Wang, L;Yang, WH;, J. Cell. Biochem. Jul 2015 ,PubMed ID 26212494 [HA]
Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice ,Zhang, J;Zhu, L;Lu, X;Feldman, ER;Keyes, LR;Wang, Y;Fan, H;Feng, H;Xia, Z;Sun, J;Jiang, T;Gao, SJ;Tibbetts, SA;Feng, P;, PLoS Pathog. Jun 2015 ,PubMed ID 26107716 [HA]
TRIM4 modulates type I interferon induction and cellular antiviral response by targeting RIG-I for K63-linked ubiquitination ,Yan, J;Li, Q;Mao, AP;Hu, MM;Shu, HB;, J Mol Cell Biol 154-63 6 2. April 2014 ,PubMed ID 24755855 [anti-HA]

DDK (FLAG) mouse monoclonal antibody, clone OTI11C3DDK（FLAG）小鼠单克隆抗体，克隆OTI11C3

发表于由
 Product Data
Product Name DDK (FLAG) mouse monoclonal antibody, clone OTI11C3
Clone Name OTI11C3
Applications ICC, IHC, WB
Target DDK (FLAG tag): DYKDDDDK
Host Mouse
Clonality Monoclonal
Immunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH
Isotype IgG1
Formulation PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 1 mg/ml
Conjugation Unconjugated
Storage Condition Store at -20°C.
Note This anti-DDK (FLAG) antibody recognizes both N-terminus and C-terminus DDK tags. It is recommended for Western blot only.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

The use of this Antibodies has been cited in the following citations:
Targeting codon 158 p53-mutant cancers via the induction of p53 acetylation ,Kong, LR;Ong, RW;Tan, TZ;Mohamed Salleh, NAB;Thangavelu, M;Chan, JV;Koh, LYJ;Periyasamy, G;Lau, JA;Le, TBU;Wang, L;Lee, M;Kannan, S;Verma, CS;Lim, CM;Chng, WJ;Lane, DP;Venkitaraman, A;Hung, HT;Cheok, CF;Goh, BC;, Nat Commun 2020 ,PubMed ID 32350249 [DDK]
Overexpression of Progerin Results in Impaired Proliferation and Invasion of Non-Small Cell Lung Cancer Cells ,Hu, XT;Song, HC;Yu, H;Wu, ZC;Liu, XG;Chen, WC;, Onco Targets Ther 2020 ,PubMed ID 32280239 [DDK]
Serine/threonine protein phosphatase 5 is a potential therapeutic target in cholangiocarcinoma ,Hu, MH;Huang, TT;Chao, TI;Chen, LJ;Chen, YL;Tsai, MH;Liu, CY;Kao, JH;Chen, KF;, Liver Int. 2018 ,PubMed ID 29797403 [DDK]
Unfractionated Heparin Alleviates Human Lung Endothelial Barrier Dysfunction Induced by High Mobility Group Box 1 Through Regulation of P38-GSK3β-Snail Signaling Pathway ,Luan, Z;Hu, B;Wu, L;Jin, S;Ma, X;Zhang, J;Wang, A;, Cell. Physiol. Biochem. 2018 ,PubMed ID 29719300 [DDK]
Inhibition of dihydrotestosterone synthesis in prostate cancer by combined frontdoor and backdoor pathway blockade ,Fiandalo, MV;Stocking, JJ;Pop, EA;Wilton, JH;Mantione, KM;Li, Y;Attwood, KM;Azabdaftari, G;Wu, Y;Watt, DS;Wilson, EM;Mohler, JL;, Oncotarget 2018 ,PubMed ID 29541409 [DDK]
TAOK1 negatively regulates IL-17-mediated signaling and inflammation ,Zhang, Z;Tang, Z;Ma, X;Sun, K;Fan, L;Fang, J;Pan, J;Wang, X;An, H;Zhou, J;, Cell. Mol. Immunol. 2018 ,PubMed ID 29400705 [DDK]
SMIT1 Modifies KCNQ Channel Function and Pharmacology by Physical Interaction with the Pore ,Manville, RW;Neverisky, DL;Abbott, GW;, Biophysical Journal 2017 ,PubMed ID 28793216 [DDK]
Exosomes expressing carbonic anhydrase 9 promote angiogenesis ,Horie, K;Kawakami, K;Fujita, Y;Sugaya, M;Kameyama, K;Mizutani, K;Deguchi, T;Ito, M;, Biochem. Biophys. Res. Commun. 2017 ,PubMed ID 28851650 [DDK]
Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA ,Martinez-Moreno, M;O'Shea, TM;Zepecki, JP;Olaru, A;Ness, JK;Langer, R;Tapinos, N;, Cell Rep 2017 ,PubMed ID 28834756 [DDK]
Involvement of p38-βTrCP-Tristetraprolin-TNFα axis in radiation pneumonitis ,Krishnamurthy, PM;Shukla, S;Ray, P;Mehra, R;Nyati, MK;Lawrence, TS;Ray, D;, Oncotarget 2017 ,PubMed ID 28548957 [DDK]
OCT4B1 regulates the cellular stress response of human dental pulp cells with ,Liu, L;Huang, R;Yang, R;Wei, X;, BioMed Research International 2017 ,PubMed ID 28473980 [DDK]
Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer ,Xiao, T;Zhu, JJ;Huang, S;Peng, C;He, S;Du, J;Hong, R;Chen, X;Bode, AM;Jiang, W;Dong, Z;Zheng, D;, Oncogene 2016 ,PubMed ID 27893713 [DDK]