标签归档：lumiprobe官方代理

Coumarin 343 azide荧光染料

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Coumarin 343 azide荧光染料

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。 产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。其中，氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。

 

上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe的完整产品线，所有规格产品保证货期5-7工作日到货，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要5-7工作日，不需要像其他家攒单导致货期3-4周。

Coumarin 343 azide荧光染料

This product will be discontinued soon in favor of Coumarin 343 X azide. We may still have some material in stock (please see availability above).

This greenish-blue emitting fluorophore can be used as a FRET donor for FAM. Azide derivative is suitable for Click Chemistry.Coumarin 343 azide structure

该蓝绿色光荧光染料可以用于FAM的FRET供体。叠氮类衍生物可以用于点击化学。

订购货号 产品名称及规格
A1430 Coumarin 343 azide, 1 mg
B1430 Coumarin 343 azide, 5 mg
C1430 Coumarin 343 azide, 10 mg
D1430 Coumarin 343 azide, 25 mg
E1430 Coumarin 343 azide, 50 mg

General properties

Appearance: yellow powder
Molecular weight: 441.48
Molecular formula: C22H27N5O5
Solubility: Good in organic solvents (DCM, chloroform, DMF, DMSO, MeCN, alcohols), poor in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 437
Extinction coefficient at excitation maximum, Lmol-1cm-1: 44000
Emission maximum, nm: 477
Fluorescence quantum yield: 0.63

Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS（水溶性）

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Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS

Sulfo-Cyanine5.5 NHS ester is an amine reactive activated ester of sulfonated far red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in purely aqueous environment, or without any significant additions of organic co-solvents.

The dye is well suitable for non-invasive in vivo NIR imaging, and for the applications requiring low fluorescent background.

水溶Cyanine 5琥珀酰亚胺酯（succinimidyl ester（SE）），类似于Cy5® NHS ester，不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。
Sulfo-Cyanine 5是Cy5®的衍生物，与很多仪器如读板机（plate readers），显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。

该染料是高度亲水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。

sulfo-Cy5.5 NHS ester structure
17320 Sulfo-Cyanine 5 .5NHS ester, 1 mg 2080.00
27320 Sulfo-Cyanine 5 .5NHS r, 5 mg 5330.00
47320 Sulfo-Cyanine 5 .5NHS , 25 mg 11050.00
57320 Sulfo-Cyanine 5 .5NHS , 50 mg 19500.00
67320 Sulfo-Cyanine 5 .5NHS , 100 mg 31850.00

General properties

Appearance: dark blue solid
Mass spec M+ increment: 1011.0
Molecular weight: 1128.41
Molecular formula: C45H44K3N3O16S4
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 675
Emission maximum, nm: 694

Product citations

  1. Zhou, Q.; Li, Z.; Zhou, J.; Joshi, B.P.; Li, G.; Duan, X.; Kuick, R.; Owens, S.R.; Wang, T.D. In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft. Photoacoustics, in press. doi: 10.1016/j.pacs.2016.04.001
  2. Abolmaali, S.; Tamaddon, A.; Kamali-Sarvestani, E.; Ashraf, M.; Dinarvand, R. Stealth Nanogels of Histinylated Poly Ethyleneimine for Sustained Delivery of Methotrexate in Collagen-Induced Arthritis Model. Pharmaceutical Research, 2015, 32(10), 3309–3323. doi:10.1007/s11095-015-1708-0

Lumiprobe FAM azide, 5-isomer

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Lumiprobe FAM azide, 5-isomer

FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.

Cat. # Quantity
14130 100 uL, 10 mM/DMSO
34130 500 uL, 10 mM/DMSO
44130 1 mL, 10 mM/DMSO
A4130 1 mg
B4130 5 mg
C4130 10 mg
D4130 25 mg
E4130 50 mg
F4130 100 mg

5-FAM azide structure

5-FAM azide

General properties

Appearance: yellowish crystals
Molecular weight: 458.42
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Su, H.; Liu, Z.; Liu, Y.; Ma, V.P.-Y.; Blanchard, A.; Zhao, J.; Galior, K.; Dyer, R.B.; Salaita, K. Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules. Nano Letters, 2018, 18(4), 2630–2636. doi:10.1021/acs.nanolett.8b00459
  2. Long, M.J.C.; Urul, D.A.; Chawla, S.; Lin, H.-Y.; Zhao, Y.; Haegele, J.A.; Wang, Y.; Aye, Y. Precision Electrophile Tagging inCaenorhabditis elegans. Biochemistry, 2018, 57(2), 216–220. doi: 10.1021/acs.biochem.7b00642
  3. Fatona, A.; Berry, R.M.; Brook, M.A.; Moran-Mirabal, J.M. Versatile Surface Modification of Cellulose Fibres and Cellulose Nanocrystals through Modular Triazinyl Chemistry. Chemistry of Materials, 2018, 30(7), 2424–2435. doi: 10.1021/acs.chemmater.8b00511
  4. Pink, M.; Verma, N.; Zerries, A.; Schmitz-Spanke, S. Dose-dependent response to 3-nitrobenzanthrone exposure in human urothelial cancer cells. Chemical Research in Toxicology, 2017, 30(10), 1855–1864. doi: 10.1021/acs.chemrestox.7b00174

上海金畔代理Lumiprobe品牌活性荧光染料产品

发表于由
上海金畔代理Lumiprobe品牌活性荧光染料产品
Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。 产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。其中，氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。
    上海金畔生物科技有限公司作为Lumiprobe中国正规签约授权代理商，Lumiprobe上海金畔生物官网：http://lumiprobe.jinpanbio.com/。可提供Lumiprobe产品，产品货期10个工作日左右，满足您的各种科研实验需求。Lumiprobe产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规，质量保证，价格优惠。
炔烃 Dye alkynes
  • 1-Ethynyl pyrene
  • 3-Ethynyl perylene
  • BDP FL alkyne
  • FAM alkyne, 5-isomer
  • FAM alkyne, 6-isomer
  • TAMRA alkyne, 5-isomer
  • BDP TMR alkyne
  • Cyanine3 alkyne
  • Cyanine5 alkyne
  • Cyanine5.5 alkyne
  • Cyanine7 alkyne
  • Cyanine7.5 alkyne
  • Sulfo-Cyanine3 alkyne
  • Sulfo-Cyanine5 alkyne
叠氮化物 Dye azides
  • Pyrene azide 1/ Pyrene azide 2
  • PEP azide
  • Perylene azide
  • BDP FL azide
  • Coumarin 343 azide
  • FAM azide, 5-isomer
  • FAM azide, 6-isomer
  • TAMRA azide, 5- isomer
  • BDP TMR azide
  • Cyanine3 azide
  • Cyanine3.5 azide
  • Cyanine5 azide
  • Cyanine5.5 azide
  • Cyanine7 azide
  • Cyanine7.5 azide
  • Sulfo-Cyanine3 azide
  • Sulfo-Cyanine5 azide
  • Sulfo-Cyanine7 azide
N-羟基琥珀酰亚胺脂Dye NHS esters
  • Pyrenebutyric acid NHS ester
  • ROX NHS ester, pure 6- isomer
  • BDP FL NHS ester
  • FAM NHS ester
  • Cyanine3 NHS ester
  • Cyanine3.5 NHS ester
  • Cyanine5 NHS ester
  • Cyanine5.5 NHS ester
  • Cyanine7 NHS ester
  • Cyanine7.5 NHS ester
  • Sulfo-Cyanine3 NHS ester
  • Sulfo-Cyanine5 NHS ester
  • sulfo-Cyanine5 bis-NHS ester
  • Sulfo-Cyanine7 NHS ester
马来酰亚胺 Dye maleimides
  • Pyrene maleimide
  • BDP FL maleimide
  • Cyanine3 maleimide
  • Cyanine5 maleimide
  • Cyanine5.5 maleimide
  • Cyanine7 maleimide
  • Cyanine7.5 maleimide
  • Sulfo-Cyanine3 maleimide
  • Sulfo-Cyanine5 maleimide
伯胺 Primary amine
  • BDP FL amine
  • Cyanine3 amine
  • Cyanine5 amine
  • Cyanine5.5 amine
  • Cyanine7 amine
  • Cyanine7.5 amine
  • Sulfo-Cyanine5 amine
  • Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
  • Pyrene hydrazide
  • BDP FL hydrazide
  • Cyanine3 hydrazide
  • Cyanine5 Boc-hydrazide
  • Cyanine5 hydrazide
  • Cyanine5.5 hydrazide
  • Cyanine7 hydrazide
  • Cyanine7.5 hydrazide
马来酰亚胺 Dye maleimides
  • BDP FL maleimide
  • Cyanine3 maleimide
  • Cyanine5 maleimide
  • Cyanine5.5 maleimide
  • Cyanine7 maleimide
  • Cyanine7.5 maleimide
  • Sulfo-Cyanine3 maleimide
  • Sulfo-Cyanine5 maleimide
羧酸 Carboxylic acids
  • Pyrenebutyric acid
  • BDP FL carboxylic acid
  • Cyanine3 carboxylic acid
  • Cyanine3.5 carboxylic acid
  • Cyanine5 carboxylic acid
  • Cyanine5.5 carboxylic acid
  • Cyanine7 carboxylic acid
  • Cyanine7.5 carboxylic acid
  • Sulfo-Cyanine3 carboxylic acid
  • Sulfo-Cyanine5 carboxylic acid
  • Sulfo-Cyanine7 carboxylic acid
点击化学试剂

非荧光炔烃 Non-fluorescent alkynes

  • Pentynoic acid STP ester
  • Biotin alkyne
非荧光叠氮化物 Non-fluorescent azides
  • Azidobutyric acid NHS ester
  • Biotin azide
寡核苷酸分析
亚磷酰胺 Phosphoramidites
  • FAM Phosphoramidite, 6-Isomer
  • Alkyne Amidite, hydroxyprolinol
  • Alkyne Phosphoramidite, 5′-terminal
  • DMS(O)MT aminolink C6
  • Long trebler phosphoramidite
  • Pyrene phosphoramidite dU
PCR/qPCR PCR/qPCR
  • ROX reference dye for qPCR
  • SYBR Green I Gel Staining Solution, 10000x
  • SYBR Green I for Real Time PCR, 100x
CPG固相修饰 CPG solid supports
  • Alkyne CPG modifier 500
  • TFA-Amino modifier CPG 500
三磷酸酯修饰 Modified triphosphates
  • Amino-11-ddUTP
  • Amino-11-dUTP
其它试剂
蛋白质组学试剂 Proteomic reagents
  • 3Dye™ 2D DIGE Kits
  • Cyanine2 NHS ester minimal dye
  • Cyanine3 NHS ester minimal dye
  • Cyanine5 NHS ester minimal dye
辅助试剂 Auxiliary reagents
  • Ascorbic acid
  • Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
  • DMF (dimethylformamide), labeling grade

Lumiprobe芘叠氮化物 1,Pyrene azide 1

发表于由

Lumiprobe芘叠氮化物 1,Pyrene azide 1

Pyrene属于多环芳烃，拥有较强短波荧光。不像其他的荧光染料，多环芳烃类属于对微环境有较强灵敏度的荧光探针。因此，它的荧光性在极性和非极性环境中是不同的。其他的影响也可以被观察到。 当两个pyrenes靠的很近时，他们便表现出激发态。激发态形式很容易被观察到，使用荧光波谱图可以被定量估测。

Pyrene属于多环芳烃，拥有较强短波荧光。不像其他的荧光染料，多环芳烃类属于对微环境有较强灵敏度的荧光探针。因此，它的荧光性在极性和非极性环境中是不同的。其他的影响也可以被观察到。
当两个pyrenes靠的很近时，他们便表现出激发态。激发态形式很容易被观察到，使用荧光波谱图可以被定量估测。
Pyrene azide可以比较容易的应用于pyrene点击化学，对难以进行炔烃标记的分子进行标记。
它也可以将分子引入难以标记pyrene的探针。
这种叠氮染料含有亲水三甘醇链以缓解其固有的疏水性并促进在水中对生物分子的标记。
另两种点击化学用pyrene azides（2和3）。

Pyrene is a polyaromatic hydrocarbon with strong short-wavelength fluorescence. Unlike other fluorescent dyes, polyaromatic hydrocarbons are fluorescent probes with strong sensitivity to microenvironment. Thus, its fluorescence is different in polar, and nonpolar environments. Other effects can also be observed.

When two pyrenes are in close proximity, they form excimers. Excimer formation can be easily observed, and quantitatively estimated using fluorescent spectra.

Pyrene azide is a reagent for easy pyrene Click Chemistry labeling of any alkyne-bearing molecule. It allows to turn any molecule into pyrene-bearing probe.

This azide contains hydrophilic triethyleneglycol linker to mitigate intrinsic pyrene hydrophobicity, and facilitate attachment to biomolecules in aqueous solutions.

Another pyrene azide for Click Chemistry labeling is also available: Pyrene azide 2.

订购货号 产品名称及规格
A1530 Pyrene azide 1, 1 mg
B1530 Pyrene azide 1, 5 mg
C1530 Pyrene azide 1, 10 mg
D1530 Pyrene azide 1, 25 mg
E1530 Pyrene azide 1, 50 mg

General properties

Appearance: yellowish solid
Molecular weight: 402.45
Molecular formula: C23H22N4O3
Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
Quality control: NMR聽1H (95%)
Storage conditions: Storage: 24 months after receival at -20掳C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 343; 326; 313; 276; 265; 242; 234
Emission maximum, nm: 377; 397

Lumiprobe Perylene azide荧光染料

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Lumiprobe Perylene azide荧光染料

Perylene是一种高亮度的且对公极其稳定的多环芳烃（PAH）类荧光染料，其量子产率接近理想化。
因其很短的荧光期，这种探针没有激发态。
因其较低的溶解度和很少量的活性衍生物，Perylene标记生物分子通常比较困难且不太容易实现。然而，通过点击化学使用这种perylene azide来进行标记就变得和其他染料一样非常容易。使用这种多环芳烃染料标记多肽、DNA、蛋白等从未如此轻松。
为了提高其亲水性和标记成功率，这种PAH azide包含亲水三甘醇链。

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with quantum yield approaching quantitative.

Due to low life time of fluorescence, this probe does not form excimers.

Perylene labeling of biomolecules is used to be difficult and tedious because of low solubility of perylene, and low availability of activated derivatives. However, labeling via Click Chemistry with this perylene azide is nearly as easy, as with other dyes. Labeling peptide, DNA, protein with this polyaromatic hydrocarbon has never been so simple yet.

To increase hydrophilicity, and facilitate labeling, this PAH azide contains hydrophilic triethyleneglycol linker.

订购货号 产品名称及规格
A5530 Perylene azide, 1 mg
B5530 Perylene azide, 5 mg
C5530 Perylene azide, 10 mg
D5530 Perylene azide, 25 mg
E5530 Perylene azide, 50 mg

General properties

Appearance: orange-yellow solid
Molecular weight: 452.50
Molecular formula: C27H24N4O3
Solubility: good in dichloromethane and chloroform, moderate in DMSO, DMF, and acetonitrile
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download